Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,942,694 A→G 8.5% L162P (CTG→CCG)  surA_2 ← Chaperone SurA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,942,6940AG 8.5% 135.8 / 4.3 47L162P (CTG→CCG) surA_2Chaperone SurA
Reads supporting (aligned to +/- strand):  ref base A (17/26);  new base G (2/2);  total (19/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01

CGCACGGATTGGCGCGCCTGCGCCTCCCGCTGCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGTCGACTGCGCTGAGCAGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCT  >  ACB122/3942580‑3942821
                                                                                                                  |                                                                                                                                
cgcACGGATTGGCGCGCCTGCGCCTCCCGCTGCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCGGTCTGTCCTGGTTGG‑CATc                                                                                                              >  6:65167/1‑134 (MQ=255)
cgcACGGATTGGCGCGCCTGCGCCTCCCGCTGCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCGGGCTGTCCTGGTTGG‑CATc                                                                                                              >  6:65169/1‑134 (MQ=255)
         tGGCGCGCCTGCGCCTCCCGCTGCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGcc                                                                                                    >  5:293991/1‑135 (MQ=255)
         tGGCGCGCCTGCGCCTCCCGCTGCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGcc                                                                                                    >  3:93278/1‑135 (MQ=255)
             gcgcctgcgcctCCCGCTGCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCa                                                                                                <  1:171399/135‑1 (MQ=255)
                 ctgcgcctCCCGCTGCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTc                                                                                            <  7:66011/135‑1 (MQ=255)
                 ctgcgcctCCCGCTGCCCGATGAGCGGTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTc                                                                                            <  8:58413/135‑1 (MQ=255)
                   gcgcctCCCGCTGCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGc                                                                                          >  5:233611/1‑135 (MQ=255)
                        tCCCGCTGCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCctcg                                                                                     <  3:23970/135‑1 (MQ=255)
                        tCCCGCTGCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTGCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCctcg                                                                                     <  5:127171/135‑1 (MQ=255)
                             cTGCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCgg                                                                                <  3:30617/135‑1 (MQ=255)
                             cTGCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCgg                                                                                <  5:240292/135‑1 (MQ=255)
                                  cGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCGGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGt                                                                           <  5:65167/135‑1 (MQ=255)
                                  cGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGt                                                                           <  6:293991/135‑1 (MQ=255)
                                  cGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGt                                                                           <  4:93278/135‑1 (MQ=255)
                                  cGAGGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCGGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGt                                                                           <  5:65169/135‑1 (MQ=255)
                                   gATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGTc                                                                          >  5:67278/1‑135 (MQ=255)
                                   gATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGTc                                                                          >  5:60522/1‑135 (MQ=255)
                                    aTGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTGCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCGCGGCGCGTCg                                                                         <  6:128665/135‑1 (MQ=255)
                                       agggTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGTCCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CTTCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGTCGACt                                                                      <  1:285777/132‑1 (MQ=255)
                                        gCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGTCGACt                                                                      <  3:288828/134‑1 (MQ=255)
                                        gCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGTCGACt                                                                      <  4:250004/134‑1 (MQ=255)
                                         cGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGTCGACTgc                                                                    <  8:20227/135‑1 (MQ=255)
                                              cccGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGTCGACTGCGCTg                                                                <  7:67265/134‑1 (MQ=255)
                                              cccGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGTCGACTGCGCTg                                                                <  6:233611/134‑1 (MQ=255)
                                                     cAAGGCCAGGCCGTGTTCGCGTATGACCAATGTATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTGGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCGCGGCGCGTCGACTGCGCTGAGCAGGGc                                                        <  1:13792/135‑1 (MQ=255)
                                                      aaGGCCAGGCCGTGTTCGCGTATGTCCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGTCGACTGCGCTGAGCAGGGc                                                        >  4:49965/1‑134 (MQ=255)
                                                           caggccGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGTCGACTGCGCTGAGCAGGGCGTacac                                                  >  4:65655/1‑135 (MQ=255)
                                                                cGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGTCGACTGCGCTGCGCAGGGCGTACACTTGCt                                             >  4:219977/1‑135 (MQ=255)
                                                                cGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGTCGACTGCGCTGAGCAGGGCGTACACTTGCt                                             >  8:229857/1‑135 (MQ=255)
                                                                cGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGTCGACTGCGCTGAGCAGGGCGTACACTTGCt                                             >  7:65665/1‑135 (MQ=255)
                                                                     tCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGTCGACTGCGCTGAGCAGGGCGTACACTTGCTGGgcc                                        >  8:283806/1‑135 (MQ=255)
                                                                     tCGCGTATGACCAATGGATTAGCCCTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CAGCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGGCGACTGCGCTGAGCAGGGCGTACACTTGCTGGgc                                         <  4:37248/134‑1 (MQ=255)
                                                                               ccATTGGATTATCACTTTGTTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑AATAAAAAAATGCCTCCACTTCCCCCTCGCTCGGCGCGTCGACTGCGCTGAGCTGGGCGTACACTTGCTGGGCCGCCATCTCa                               <  2:83073/134‑1 (MQ=255)
                                                                               ccACTGGATTATCCCTTTGTTGCTGCGCTCCTTGCAGTGGGT‑CGGGTGGGCCAAAAAAAAATCCCGCCCCTTCCCCCTGGCGCGGCGCGGCGCCGGGGCTGCGCCGGGCGTACACTTGCTGGGCCGCCATCTCa                               <  6:164734/134‑1 (MQ=255)
                                                                               ccAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGTCGACTGCGCTGAGCAGGGCGTACACTTGCTGGGCCGCCATCTCAt                              >  8:132608/1‑135 (MQ=255)
                                                                                 aaTGTATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTGGG‑AATCAAAAAATGCCTCCACTCCGCCCGCGCGCGGCGCGTCGACTGCGCTGAGCAGGGCGTACACTTGCTGGGCCGCCATCTCATGc                            <  3:49965/135‑1 (MQ=255)
                                                                                         aTCAATTTGGTTCTGCGGGCGTTGAAGTCTGGCGTGGGTGG‑CATCAAAAACTGCCTCCAGTTCGCCCTCGCGGGGCGGGGCTACTGCGCTGAGCAGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGgtgt                     <  6:60522/134‑1 (MQ=255)
                                                                                          tCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGTCGACTGCGCTGAGCAGGGCGTACACTTGCTGGGCCGCCATCTCa                               >  3:212359/1‑123 (MQ=255)
                                                                                          tCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑AATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGTCGACTGCGCTGAGCAGGGCGTACACTTGCTGGGCCGCCATCTCa                               <  4:212359/123‑1 (MQ=255)
                                                                                           cACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTGGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGGCGACTGCGCTGAGCAGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTAt                   <  5:144560/134‑1 (MQ=255)
                                                                                                tGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCctcg                                                                                     >  7:301348/1‑63 (MQ=255)
                                                                                                tGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCctcg                                                                                     <  8:301348/63‑1 (MQ=255)
                                                                                                   ttCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGTCGACTGCGCTGAGCAGGGCGTACACTTGCTGGGCCGCCATCTCa                               <  3:242482/114‑1 (MQ=255)
                                                                                                   ttCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGTCGACTGCGCTGAGCAGGGCGTACACTTGCTGGGCCGCCATCTCa                               >  4:242482/1‑114 (MQ=255)
                                                                                                   ttCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGTCGACTGCGCTGAGCAGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTAc          >  3:124279/1‑135 (MQ=255)
                                                                                                           tCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGTCGACTGCGCTGAGCAGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCt  >  4:123204/1‑135 (MQ=255)
                                                                                                                  |                                                                                                                                
CGCACGGATTGGCGCGCCTGCGCCTCCCGCTGCCCGATGAGCGTTGCCCGAGCCAAGGCCAGGCCGTGTTCGCGTATGACCAATGGATTATCACTTTGGTTCTGCGCTCCTTGCAGTCTGTCCTGGTTGG‑CATCAAAAAATGCCTCCACTTCGCCCTCGCTCGGCGCGTCGACTGCGCTGAGCAGGGCGTACACTTGCTGGGCCGCCATCTCATGCCGGGTGTATTCAGTGTACTGCGCCCT  >  ACB122/3942580‑3942821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑