Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 4,097,408 C→A 7.3% S101Y (TCC→TAC)  cntO_6 → Metal‑pseudopaline receptor CntO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1224,097,4080CA 7.3% 176.5 / 4.4 55S101Y (TCC→TAC) cntO_6Metal‑pseudopaline receptor CntO
Reads supporting (aligned to +/- strand):  ref base C (29/22);  new base A (2/2);  total (31/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01

GACGCGCCGCTGCGCGATATCCCGCAAACCGTCAACGTCGTTCCCGAAAGCGTGATCAAGGACCAAGGCGCACAGTCCATGGAAGACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTCGCGATCTGTCCAACATCGA  >  ACB122/4097290‑4097525
                                                                                                                      |                                                                                                                     
gACGCGCCGCTGCGCGATATCCCGCAAACCGTCAACGTCGTTCCCGAAAGCGTGATCAAGGACCAAGGCGCACAGTCCATGGAAGACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGCCGGCCaa                                                                                                       >  2:152580/1‑135 (MQ=255)
  cgcgCCGCTGCGCGATATCCCGCAAACCGTCAACGTCGTTCCCGAAAGCGTGATCAAGGACCAAGGCGCACAGTCCATGGAAGCCGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAAcg                                                                                                     >  7:61223/1‑135 (MQ=255)
  cgcgCCGCTGCGCGATATCCCGCAAACCGTCAACGTCGTTCCCGAAAGCGTGATCAAGGACCAAGGCGCACAGTCCATGGAAGACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAAcg                                                                                                     >  1:184532/1‑135 (MQ=255)
          tGCGCGATATCCCGCAAACCGTCAACGTCGTTCCCGAAAGCGTGATCAAGGACCAAGGCGCACAGTCCATGGAAGACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAgg                                                                                             >  5:224763/1‑135 (MQ=255)
                         aaaCCGTCAACGTCGTTCCCGAAAGCGTGATCAAGGACCAAGGCGCACAGTCCATGGAAGACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGt                                                                                            <  5:148517/121‑1 (MQ=255)
                         aaaCCGTCAACGTCGTTCCCGAAAGCGTGATCAAGGACCAAGGCGCACAGTCCATGGAAGACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGt                                                                                            >  6:148517/1‑121 (MQ=255)
                                    gtcgtTCCCGAAAGCGTGATCAAGGACCAAGGCGCACAGTCCATGGAAGACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCAtt                                                                   <  1:13292/135‑1 (MQ=255)
                                        ttCCCGAAAGCGTGATCAAGGACCAAGGCGCACAGTCCATGGAAGACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCg                                                               <  5:74630/135‑1 (MQ=255)
                                                   gTGATCAAGGACCAAGGCGCACAGTCCATGGAAGACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTAc                                                       >  8:248338/1‑132 (MQ=255)
                                                                aatgcgcACAGTCCATGGATGACGTGCTGAAGAACGTGCCCGGCATAGGCATGTCCAACGGCTACGGCCAACTAGATCAGGTGACCATCCGTGGCTTCTGCGCCATTGGAGACATGTCCATAGACGGcgtgcgtg                                       <  5:283962/132‑1 (MQ=255)
                                                                aaGGCGCACAGTCCATGGAAGACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGcgtgcgtg                                       <  2:13214/135‑1 (MQ=255)
                                                                          gTCCATGGAAGACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCt                             <  7:112318/135‑1 (MQ=255)
                                                                             cATGGAAGACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTa                          >  2:151447/1‑135 (MQ=255)
                                                                              aTGGAAGACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACTCCCTGTAc                         >  6:283962/1‑135 (MQ=255)
                                                                              aTGGAAGACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTAc                         >  5:137316/1‑135 (MQ=255)
                                                                              aTGGAAGACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTACAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGcgtgcgtg                                       <  7:178392/121‑1 (MQ=255)
                                                                              aTGGAAGACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTACAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGcgtgcgtg                                       <  7:173876/121‑1 (MQ=255)
                                                                              aTGGAAGACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTACAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGcgtgcgtg                                       >  8:173876/1‑121 (MQ=255)
                                                                              aTGGAAGACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTACAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGcgtgcgtg                                       >  8:178392/1‑121 (MQ=255)
                                                                               tGGAAGACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACCTCGACGGCGTGCGTGACGACGCCCTGTACt                        <  2:2890/135‑1 (MQ=255)
                                                                                ggAAGACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCAtt                                                                   <  5:120298/91‑1 (MQ=255)
                                                                                ggAAGACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCAtt                                                                   >  6:120298/1‑91 (MQ=255)
                                                                                ggAAGACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACtt                       >  4:104151/1‑135 (MQ=255)
                                                                                  aaGACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTc                     >  4:45670/1‑135 (MQ=255)
                                                                                   aGACGTGCTGATGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTcg                    >  6:3682/1‑135 (MQ=255)
                                                                                    gccGTGGTGACGAACGGGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTcgc                   <  1:152580/133‑1 (MQ=255)
                                                                                    gACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTcgc                   <  3:104151/135‑1 (MQ=255)
                                                                                    gACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTcgc                   <  6:137316/135‑1 (MQ=255)
                                                                                    gACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTcgc                   <  2:184532/135‑1 (MQ=255)
                                                                                    gACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTcgc                   <  5:42825/135‑1 (MQ=255)
                                                                                    gACGTGCGGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTcgc                   <  1:291132/135‑1 (MQ=255)
                                                                                        tGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAgcg                                                                        <  2:47271/78‑1 (MQ=255)
                                                                                        tGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAgcg                                                                        >  1:47271/1‑78 (MQ=255)
                                                                                        tGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCg                                                               <  1:259881/87‑1 (MQ=255)
                                                                                        tGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCg                                                               >  2:259881/1‑87 (MQ=255)
                                                                                        tGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGcgtgcgtg                                       >  7:13441/1‑111 (MQ=255)
                                                                                        tGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGcgtgcgtg                                       <  8:13441/111‑1 (MQ=255)
                                                                                        tGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTcgc                   <  1:168756/131‑1 (MQ=255)
                                                                                        tGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTcgc                   >  2:119606/1‑131 (MQ=255)
                                                                                        tGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTcgc                   <  1:47010/131‑1 (MQ=255)
                                                                                        tGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTcgc                   >  2:168756/1‑131 (MQ=255)
                                                                                        tGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTcgc                   <  1:119606/131‑1 (MQ=255)
                                                                                        tGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTcgc                   >  2:47010/1‑131 (MQ=255)
                                                                                        tGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTCGCGATc               >  7:178251/1‑135 (MQ=255)
                                                                                        tGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTCGCGATc               >  4:50441/1‑135 (MQ=255)
                                                                                        tGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTCGCGATc               >  6:170095/1‑135 (MQ=255)
                                                                                        tGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTCGCGATc               >  4:250727/1‑135 (MQ=255)
                                                                                        tGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTCGCGATc               >  3:139540/1‑135 (MQ=255)
                                                                                        tGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTCGCGATc               >  2:281431/1‑135 (MQ=255)
                                                                                        tGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTCGCGATc               >  2:21105/1‑135 (MQ=255)
                                                                                        tGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTCTACTTTCGCGATc               >  5:18649/1‑135 (MQ=255)
                                                                                                  cGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTCGCGATCTGTCCAACAt     <  1:151447/135‑1 (MQ=255)
                                                                                                     gCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTCGCGATCTGTCCAACATCGa  >  7:302615/1‑135 (MQ=255)
                                                                                                          gCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTCGCGATCTGTCCaa        >  7:52566/1‑124 (MQ=255)
                                                                                                          gCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTCGCGATCTGTCCaa        <  8:52566/124‑1 (MQ=255)
                                                                                                                      |                                                                                                                     
GACGCGCCGCTGCGCGATATCCCGCAAACCGTCAACGTCGTTCCCGAAAGCGTGATCAAGGACCAAGGCGCACAGTCCATGGAAGACGTGCTGAAGAACGTGCCCGGCATCGGCCTGTCCAACGGCGACGGCCAACGCGATCAGGTGACCATCCGTGGCTTCAGCGCCATTGGCGACATGTACATAGACGGCGTGCGTGACGACGCCCTGTACTTTCGCGATCTGTCCAACATCGA  >  ACB122/4097290‑4097525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑