| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 4,760,742 | A→C | 32.8% | H41P (CAC→CCC) | vanB → | vanillate O‑demethylase oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 4,760,742 | 0 | A | C | 32.8% | 36.9 / 5.5 | 37 | H41P (CAC→CCC) | vanB | vanillate O‑demethylase oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (7/17); new base C (10/3); total (17/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.54e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGC > ACB122/4760616‑4760874 | ccGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCCGCGCCGGCGCCCATATCGACGTGCACCAGcccc > 4:6763/1‑134 (MQ=255) cGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCCTATCGACGTGCCCCAGcccca > 6:11790/1‑133 (MQ=255) aCATGATCGAGGCCGTAGGGGTATCCCGTAACGATGAAGCGCAGGGTAGCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACg < 1:226279/135‑1 (MQ=255) cGATGCCGTCGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCTGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGt < 7:229440/135‑1 (MQ=255) gATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGGCCCTGCCCCACCGGCggggg > 8:250312/1‑133 (MQ=255) tGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCCCCAGCCCGCCCGGCGGGTgcg > 1:159281/1‑135 (MQ=255) tGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACCGGCGGGTgcg > 7:169134/1‑135 (MQ=255) gTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGGCCCGGCCCCACCGGCCGGTgggcgc > 3:279900/1‑132 (MQ=255) tAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGt < 6:29967/135‑1 (MQ=255) tGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTAt < 6:284910/134‑1 (MQ=255) cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCCCCAGCCCGCCCGGCTGGGGGGCCAGGATTAGCggtg > 1:190734/1‑134 (MQ=255) cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCagtg > 4:105504/1‑134 (MQ=255) cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCGGGTGCGCCAGTATTCGCtgtg > 4:211386/1‑134 (MQ=255) cGTAACGAGGAACCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGGTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGc < 2:295352/135‑1 (MQ=255) tAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGCGCTGGTGCGCCCCTAATCGCTGTGcca > 7:218971/1‑133 (MQ=255) cGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCCCCTGCCCGCCGGGCTGGTGCGCCAGTATTAGCTGTGccacca > 8:106824/1‑135 (MQ=255) cGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGAGCTGGTGCGCCAGTATTAGCTGGGCAacca > 5:113453/1‑135 (MQ=255) cgcAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCCACCGGCCGACCGGCTGGTGGCCCCGTTTTAGGTTTGCAACCACCCCgaag > 4:296987/1‑135 (MQ=255) cAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGCCGTGGCCCGGGCCGCCCGGCGGGGGGGGCCGGATTGGCTGTGGGACCCCCCCCCAGAACGcccacgcg > 5:64769/1‑128 (MQ=255) gCTTGGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATc < 1:69106/135‑1 (MQ=255) gCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATc < 4:297354/135‑1 (MQ=255) gAGCGGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTGCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGa < 1:294820/133‑1 (MQ=255) aGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTg < 6:108417/135‑1 (MQ=255) gCTGTCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGAGCCCATATCGACGTGCCCCGGCCCCACGGGCGGGTGCGCCAGAATTCTCTGTGCTAACACACCCAAGAAATCCATCGCGATGTGagttt > 8:300567/1‑131 (MQ=255) tGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCCCCAGGCCGCCGGGCTGGTGCGCCAGTATTCGCGGTGCCACCCCCCCGAAGAAAGCCCTCGCGATAGGATTGGCg > 7:151835/1‑135 (MQ=255) aGCCTGCTGCCGGGGTTCAGCGGCGGCGCCCATATCGCCCGGCCCCTGCCCGTCGGGGCGGGGGGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCt < 7:250312/135‑1 (MQ=255) gcCGGCGTTCTGCGCCGGGGCCCATATCGCGGTGCACCTGCCCGCCGGGAGGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgggg < 4:164307/135‑1 (MQ=255) gcCGGCGTTCCGCGCGGGCGCCCATCTCGACGTGCCCCTGCCCGCCGGGCTGGTGCGCCAGTATTCGCTGTGCAACCCCCCCGAAGAACGCCATCGCTATATGATTGGCGTACTCCACGACCCGGCTTCGCgggg < 3:6763/135‑1 (MQ=255) gcCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCCCCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgggg < 8:17531/135‑1 (MQ=255) cGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgggg < 3:290859/133‑1 (MQ=255) cGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgggg < 7:159406/133‑1 (MQ=255) cGGCGTTCAGCGCCGGCGCCCATATCGACGGGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgggg < 2:171747/133‑1 (MQ=255) cGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCGGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGtt < 6:36762/135‑1 (MQ=255) tCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTtctc < 1:249558/135‑1 (MQ=255) gcCCATAGCGACGTGCACCGGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCAc < 3:46522/135‑1 (MQ=255) cgggCACCTGCCCTCCGGGCGGGTGCGCCAGTATTCGCTGTGCAACCACCCCGCAGAACGCCATCGCTATCTGCTTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCa < 3:37747/132‑1 (MQ=255) gCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTTCTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGc > 5:47743/1‑135 (MQ=255) | CCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGC > ACB122/4760616‑4760874 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |