Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | ACB122 | 4,874,709 | A→C | 41.5% | intergenic (+13/‑174) | CJ019_04568 → / → uacT_1 | hypothetical protein/Uric acid transporter UacT |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | ACB122 | 4,874,709 | 0 | A | C | 41.5% | 19.6 / 2.3 | 25 | intergenic (+13/‑174) | CJ019_04568/uacT_1 | hypothetical protein/Uric acid transporter UacT |
Reads supporting (aligned to +/- strand): ref base A (5/9); new base C (6/5); total (11/14) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.35e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCA > ACB122/4874578‑4874832 | gcATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAACGCGCCCAGGTCAACTGACAGCACCGCGCCCgg > 7:17678/1‑134 (MQ=255) ttCAGGCGCATGGGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGACGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAAc < 4:202448/135‑1 (MQ=255) aGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACAGAAGAGCGCAAGCTCGTCGGCGACTGGCTCGCGACAGGCGCCCCGGGCAAATGAAACCACCTCGCCACGCCACCCg > 2:113332/1‑135 (MQ=255) ggTTGCCAGCCAGATAATGCCGCTGGGAAACATCACCAATATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAgg < 2:246599/135‑1 (MQ=255) gccatATCAGGCCGCGGGGCAACACCACCCACAGCACCCCCTACGCGCGCACGCTCGGCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCAcc < 1:237687/135‑1 (MQ=255) gccagAGCATGCCGGGGGCAAACAACACCCATATCCCCACCGAACCGCGCATGGGGGGCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCAcc < 1:1693/135‑1 (MQ=255) cATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGCCAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTACCCCt > 6:296530/1‑135 (MQ=255) cggCTGGCCAACATCACCCAGCTGACCCCCCAAGCGCGAAAGCGGGGCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCa < 2:182852/132‑1 (MQ=255) gCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCCACTGCCAGCACCTCGCCCGGCAACCCGCCCCCCAAGGGAATCCCCTTCCCCTGCAGGa > 4:132715/1‑135 (MQ=255) gCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATAGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCCCCGTCCCCGGCAGGAGCggg > 8:232886/1‑135 (MQ=255) ccAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGCCAGCACCACGCCCGGCAACCCCCCCCCCCAGGGAATCACCGTCCCCGGCAGGAGCGGGCGTGCCcgcg > 3:171783/1‑135 (MQ=255) aGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACGGGATCGGCAAAGGCGCCCAGGTGAACTGACCGCACCTAGCCCGGCAACCCGCCCCCCCAGGGCATCACCTTCCCCTGCCGGAGCGGGCTTGccccc > 2:10978/1‑130 (MQ=255) accaccGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCCGGCAACCCGCCCCCCCAGGGAATCACCGTCCCCGGCAGGCGCGGGCTTGCCCGCGAAGAGGc > 6:117285/1‑135 (MQ=255) accaccGAAGAGCGCAAGCTCGTCGGCGACTGGAACGCCAAAGGCGCCCAGGTCAACAGACAGCACCACCCCCCGCAACCCCCCCCCCCAGGGAATAACCTACCCCTCCAGGAGCCGGCTTGCCCCCGAACAggg > 2:274229/1‑134 (MQ=255) cacggtcggcgGCGACTGGAGCGCCAACGGCGCCCAGGTAAACTGACAGCACCGCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGCGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGcc < 4:117298/130‑1 (MQ=255) cgtcgGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGc < 8:30339/135‑1 (MQ=255) aCTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCAtgtg < 4:276721/135‑1 (MQ=255) agccccAACGGCGCCCAGGACAACTCCCCGCCCCGCGCCCGGCACCCCGCCCCCCACGGCAACCCCCTCCCCCTGCCGGCGGGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACg < 5:117285/131‑1 (MQ=255) ccaagcgcgcCCCGGCCAACTGACCCCCCCCCCCCCGCCACCCCCCCCCCCAGGGGACTCACCTCCCCCTGCCGGCGGGGGGTTGCCCGCGAACAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGcaac < 6:181340/129‑1 (MQ=255) gcCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACaagcaagc > 3:100197/1‑135 (MQ=255) tCAACTGACAGCCCCGCGCCACGCAACCCGCCCCCCACGGGAATCACCTCCCCCTGCCGGCGCGGGCTTGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc < 2:168505/135‑1 (MQ=255) acaccTGCCAGCACCCCGCCCGGCACCCCCCCCCCCCTGGTAATCACCTCCCCCTGCTGGAGCGGGGTTGCCCGCTAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc < 1:274229/131‑1 (MQ=255) acAACTGACCGCACCGCGCCCGCCACCCCCCCCCCCACGGTAAGCACCTTCCCCTGCTGGAGCGGGGTTGCCCGCTACGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgc < 1:10978/134‑1 (MQ=255) aaCTGACAGCACCTCGCCCGGCAACCCGCCCCCCACGGGAACCACCTTCCCCTGCAGGCGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAgcgc < 1:55142/135‑1 (MQ=255) gCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCa > 5:95801/1‑134 (MQ=255) | GCATTCAGGCGCAAGCGGTTGCCAGCCAGATCATGCCGCTGGGCAACATCACCCAGATGACCACCGAAGAGCGCAAGCTCGTCGGCGACTGGATCGCCAAAGGCGCCCAGGTCAACTGACAGCACCTCGCCAGGCAACCCGCCCCCCAAGGGAATCACCTTCCCCTGCAGGAGCGGGCTTGCCCGCGAAGAGGCCGATAGCCCCAGCCTCAAGCTCCATGTGTTACGCAACAAGCAAGCATCGAGCGCGCAGGCA > ACB122/4874578‑4874832 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |