| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 740,674 | T→G | 50.5% | L38R (CTG→CGG) | CJ019_00704 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 740,674 | 0 | T | G | 50.5% | 1.5 / 10.3 | 21 | L38R (CTG→CGG) | CJ019_00704 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (7/2); new base G (8/3); total (16/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.33e-01 | |||||||||||
GGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCTGTTTGTTTCCAGCTATGAGGTGGTCAGCTTCTATTGCGCGATCCGT > ACB122/740552‑740802 | ggaTCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGGTGTGGGTGGTGGGGGGGGGGCGGGGGGTTGTggg > 7:149481/1‑134 (MQ=255) gagaACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGGTGTGGGTGGTGGGGGGGGCGCGGTTGGGTGTGGGTGAAAGGGTGGa > 6:294339/1‑135 (MQ=255) ggTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTTGTGGGGGGGGCGCTGTGGGGTCTGGGGGAATGGCGGGAGCGGAg > 1:43962/1‑135 (MQ=255) tgttgtGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCAGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGAGCTGTGGGGTGTTGATGAATGGCGGGAGCGGAGGGGgc > 4:84116/1‑135 (MQ=255) ggttgtGGCTGGTGCTTGCACTGGCTGGGTGGTGTGTGGGCTCTCATGGGCCCACACCACTCTGGGGCTTCGGGGTGGTGTGGGTGGGGGGGGGGGCGCGGTGGGTGCTGGCTGAATGGCTGGAGCGGAggggg < 6:237774/133‑1 (MQ=255) tGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGGTGTGGGTGGTGGGGGGGGGGGGGTGGGTGGTTGCGGATTGGGGGGTTGGGCGGGGGCGCTATCCatg > 1:186746/1‑135 (MQ=255) tGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGGTGTGGGGGGTGGGGGGGGGGCGGGGGGTTGGGGGGGGATGGGGGGGGCGGCGGGGGGGccatcaatt > 5:45815/1‑134 (MQ=255) tGGCCTTGCAAGTGCACGCTTCTGTCTCGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGAGGGAGGTGGGGGGGGCGCGGTGGGTTGATGCTGAATGAGTGGACCGGATGGGGCGGAATCAatg > 4:11556/1‑135 (MQ=255) tCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCAGTGGGTTCGGGCTGAATGGCGGGGGCGGAGGGGGCGCAATCAATGATGCGCGCCCATCTAGACGa > 3:129242/1‑135 (MQ=255) tctcACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTGGGt < 6:150875/65‑1 (MQ=255) tctcACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCGGTGGGt > 5:150875/1‑65 (MQ=255) tGGCCGACACCACTCTGGGTCATCGTCGTGGTGTGGGTGGTGGGGGGGGCGCGGTGGGTTGTGGGGGATTGGGGGGCGCCGGGGGGGCGGCATCCATGATGGGCGCCCATAGAGACGAGCTGgtggtggtggggt > 1:230372/1‑131 (MQ=255) gACACCACTCTGGGTCATCGTCGTGGTGGGGGTGGTGGGGGGGGGGCGGGGGGGTGTGGCTGGATTGGTGGTGGGGGGGGGGGGGCATCACTGATGCGCGCCCATCTTGACGCGCTGGTGAGCGTGAGCggtgtg > 6:5842/1‑135 (MQ=255) tGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTTGGTTATGGCTGCATGGCTGGAGCCGCCGGGGCCCAATCAATTATGCGCGCCCAAAAATAAGAGCTGGTGTTATTTAGCTGTTTTTTTCCAGCTa > 2:58434/1‑135 (MQ=255) tcgtGTTGTGGGTGGTGGGGGGGGCGCTGTGGGGTCTGGCTGAATGGCTGGCGCGGAGGGGGCGCAATCAATGATTCGCGCCAATCTAGACGAGCGGGTGATCGTGAGCTGTTTGTTTACAGCTATGAggtgg > 6:59086/1‑133 (MQ=255) cgtGTTGTGGGTGGTGGGGGGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGCGGGGGCGCAAACAATTATGCGCGCCAATATAGAAGAGCTGGTGTTCGTGAGCTGTTTTTTTCCAGCTATGAGGTGGTCa > 8:11738/1‑135 (MQ=255) ggtggGGGGGGCGCGGGGGGGGCTGGCTGAAGGGCTGGAGCGGCGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCTGTTTGTTTCCAGCTATGAGGTGGTCAGCTTCTATTgcg < 8:149481/135‑1 (MQ=255) ggggggggCGCTGTGGGTTCTGGCTGAATGGCTTGAGAGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCTGTTTGTTTCCAGCTATGAGGTGGTCAGCTTCTATTg > 8:86704/1‑130 (MQ=255) ggggggggCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCTGTTTGTTTCCAGCTATGAGGTGGTCAGCTTCTATTGCGCGa > 2:288649/1‑135 (MQ=255) gggCGGTGTGGGTGCGGGCTCAACGGCTGGAGCGCAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCTGTTTGTTTCCAGCTATGAGGTGGTCAGCTTCTATTGCGCGATCCGt < 3:11556/135‑1 (MQ=255) cggCGCGGGGGGTGCTGGCTCACTGGCGGGAGCGGCGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCTGTTTGTTTCCAGCTATGAGGTGGTCAGCTTCTATTGCGCGATCCGt < 7:11738/134‑1 (MQ=255) | GGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCTCTCACTGGCCGACACCACTCTGGGTCATCGTCGTGTTGTGGGTGGTGGGGGGGGCGCTGTGGGTTCTGGCTGAATGGCTGGAGCGGAGGGGGCGCAATCAATGATGCGCGCCAATCTAGACGAGCTGGTGATCGTGAGCTGTTTGTTTCCAGCTATGAGGTGGTCAGCTTCTATTGCGCGATCCGT > ACB122/740552‑740802 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |