Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	5,160,922	C→T	6.4%	R309H (CGT→CAT)	CJ019_04854 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	5,160,922	0	C	T	6.4%	183.6 / 2.6	62	R309H (CGT→CAT)	CJ019_04854	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (26/32);  new base T (2/2);  total (28/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCACCACGCGGCGCCCGGTGTGCGGTTGGAAGATACGCTGCTGCTCGGCCGGTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGGGTGGCAAGGATGGCCTGATCGACGGTGAGGTCGTCCTCGATCTGGT  >  ACB122/5160796‑5161054                                                                                                                               |                                                                                                                                     aGCACCACGCGGCGCCCGGTGTGCGGTTGGAAGATACGCTGCTGCTCGGCCGGTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTcc                                                                                                                              <  6:263760/135‑1 (MQ=255)   cacctcGCGGCGCCCGGTGTGCGGTTGGAAGATACGCTGCTGCTCGGCCGGTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCg                                                                                                                            <  5:15098/135‑1 (MQ=255)   caccacGCGGCGCCCGGTGTGCGGTTGGAAGATACGCTGCTGCTCGGCCGGTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCg                                                                                                                            >  8:278265/1‑135 (MQ=255)   caccacGCGGCGCCCGGTGTGCGGTTGGAAGATACGCTGCTGCTCGGCCGGTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCg                                                                                                                            >  8:278259/1‑135 (MQ=255)      cacGCGGCGCCCGGTGTGCGGTTGGAAGATACGCTGCTGCTCGGCCGGTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCACGCTc                                                                                                                         >  8:179478/1‑135 (MQ=255)        cgcgGCGCCCGGTGTGCGGTTGGAAGATACGCTGCTGCTCGGCCGGTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGc                                                                                                                       >  5:87882/1‑135 (MQ=255)           ggcgCCCGGTGTGCGGTTGGAAGATACGCTGCTGCTCGGCCGGTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCg                                                                                                                    >  8:295242/1‑135 (MQ=255)           ggcgCCCGGTGTGCGGTTGGAAGATACGCTGCTGCTCGGCCGGTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCg                                                                                                                    >  2:175272/1‑135 (MQ=255)           ggcgCCCGGTGTGCGGTTGGAAGATACGCTGCTGCTCGGCCGGTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCg                                                                                                                    >  5:93944/1‑135 (MQ=255)           ggcgCCCGGTGTGCGGTTGGAAGATACGCTGCTGCTCGGCCGGTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCg                                                                                                                    >  5:85804/1‑135 (MQ=255)             cgcCCGGTGTGCGGTTGGAAGATACGCTGCTGCTCGGCCGGTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGc                                                                                                                  <  8:225577/135‑1 (MQ=255)             cgcCCGGTGTGCGGTTGGAAGATACGCTGCTGCTCGGCCGGTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGc                                                                                                                  <  3:291561/135‑1 (MQ=255)                         ggTTGGAAGATACGCTGCTGCTCGGCCGGTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGc                                                                                                      <  7:114179/135‑1 (MQ=255)                               aaGATACGCTGCTGCTCGGCCGGTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCg                                                                                                <  8:41246/135‑1 (MQ=255)                               aaGATACGCTGCTGCTCGGCCGGTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCg                                                                                                <  8:145426/135‑1 (MQ=255)                                aGATACGCTGCTGCTCGGCCGGTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGc                                                                                               <  2:44319/135‑1 (MQ=255)                                aGATACGCTGCTGCTCGGCCGGTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATATCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCga                                                                                               >  7:275918/1‑134 (MQ=255)                                      gctgctgctCGGCCGGTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGc                                                                                         <  8:135489/135‑1 (MQ=255)                                       ctgctgctCGGCCGGTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGcc                                                                                        >  6:63858/1‑135 (MQ=255)                                        tgctgctCGGCCGGTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATAACTGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGccc                                                                                       >  1:224257/1‑135 (MQ=255)                                          ctgctCGGCCGGTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCtt                                                                                     <  4:74823/135‑1 (MQ=255)                                            gctCGGCCGGTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGc                                                                                   >  1:122824/1‑135 (MQ=255)                                              tCGTCCGGTGACAGGCTCGCGTACAGCGGCAAAATCTCGGTATGTCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACTGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCCCCCGGGCAATTGccc                                                                                 <  8:275918/135‑1 (MQ=255)                                                     gTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGc                                                                          >  3:187401/1‑135 (MQ=255)                                                         cAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTc                                                                      <  5:101752/135‑1 (MQ=255)                                                               cgcgTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCATGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGtgctg                                                                >  6:162475/1‑135 (MQ=255)                                                               cgcgTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCATGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGGTCGtgcgg                                                                >  6:162477/1‑133 (MQ=255)                                                                  gTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTgcgc                                                             <  2:301710/135‑1 (MQ=255)                                                                   tACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTgcg                                                              >  5:21493/1‑133 (MQ=255)                                                                           cAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCtcgtc                                                    <  5:98959/135‑1 (MQ=255)                                                                            aaaaTCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCtcgtcg                                                   <  1:175272/135‑1 (MQ=255)                                                                            aaaaTCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCtcgtcg                                                   <  1:29662/135‑1 (MQ=255)                                                                             aaaTCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGa                                                  >  2:203851/1‑135 (MQ=255)                                                                                tctcGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCCCGTCGAggg                                               <  4:29071/135‑1 (MQ=255)                                                                                     gTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCGTTGCCCTCGCTGCGGTCGTGCTGCGGCAGCTCGTGGCGGGAgg                                            >  1:117434/1‑133 (MQ=255)                                                                                     gTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGGGt                                              <  3:124484/131‑1 (MQ=255)                                                                                           cgcAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGGGTGGCAAGGAt                                     <  8:260749/134‑1 (MQ=255)                                                                                           cgcAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGGGTGGCAAGGAt                                     <  2:224347/134‑1 (MQ=255)                                                                                            gcAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGGGTGGCAAGGATgg                                   >  5:117327/1‑135 (MQ=255)                                                                                               gTTGCGCCTTGCGCAGGATCTCGGCGGCATCATGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGGGTGGCAAGGATGGCCt                                <  5:162475/135‑1 (MQ=255)                                                                                               gTTGCGCCTTGCGCAGGATCTCGGCGGCATCATGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTGCGTGCTGCGCCAGCTCGTCGAGGGTGGCAAGGATGGCCt                                <  5:162477/135‑1 (MQ=255)                                                                                               gTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGGGTGGCAAGGATGGCCt                                <  3:231553/135‑1 (MQ=255)                                                                                                ttGCGCCTTGCGCAGGTTGTCGGCGGCTTCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCTCATCGCCCGGGCCCTTGCCCTCGCTGCGGTGGTGCTGCGCCAGGTCGTCGAGGGTGGCAAGGATGGCCt                                <  6:210936/134‑1 (MQ=255)                                                                                                ttGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGGGTGGCAAGGATGGCCTg                               <  6:87882/135‑1 (MQ=255)                                                                                                 tGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGTAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGGGTGGCAAGGATGGCCTg                               <  4:187401/134‑1 (MQ=255)                                                                                                  gcgcCTTGCGCAGGATCTCGGCGGCATCACGGAGTTCCCGCTCGCCCGGCAGGAAGATCATCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGGGTGGCAAGGATGGCCTGAt                             <  6:219464/135‑1 (MQ=255)                                                                                                       ttGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGTGTGGCAAGGATGGCCTGATCGACg                        <  7:295242/135‑1 (MQ=255)                                                                                                        tGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGGGTGGCAAGGATGGCCTGATCGACgg                       >  7:271466/1‑135 (MQ=255)                                                                                                        tGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGGGTGGCAAGGATGGCCTGATCGACgg                       >  4:90415/1‑135 (MQ=255)                                                                                                           gcAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGGGTGGCAAGGATGGCCTGATCGACGGTGa                    >  4:290665/1‑135 (MQ=255)                                                                                                             aGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGACCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGGGTGGAAAGGATGGCCTGATCGACGGTGAgg                  >  7:106652/1‑135 (MQ=255)                                                                                                             aGGATCTCGGCGGCATCACGGATATCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGATCGTGCTGCGCCAGCTCGTCGAGGGTGGCAAGGATCGCCTGATCGACGGTGacg                  >  5:210936/1‑133 (MQ=255)                                                                                                               gATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGGGTGGCAAGGATGGCCTGATCGACGGTGAGgtc                <  7:59703/135‑1 (MQ=255)                                                                                                                aTCTCGGCGTCTTCACTGATTTCCCGGGCGGCCGGCAGGAAGATCAGCACATCGCCCGGGCGCTTGCCCTCGCTGCGTGGGTGCTGCGCCAGCTCGTCGTGGGTGGCAAGGATGGCCTGATCGACGGTGAGgtc                <  1:302347/134‑1 (MQ=255)                                                                                                                       cggcATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGGGTGGCAAGGATGGCCTGATCGACGGTGAGGTCGTCCTCg         <  8:271509/134‑1 (MQ=255)                                                                                                                       cggcATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGGGTGGCAAGGATGGCCTGATCGACGGTGAGGTCGTCCTCg         >  7:271509/1‑134 (MQ=255)                                                                                                                       cggcATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGGGTGGCAAGGATGGCCTGATCGACGGTGAGGTCGTCCTCGa        >  6:1343/1‑135 (MQ=255)                                                                                                                            tCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGGGTGGCAAGGATGGCCTGATCGACGGTGAGGTCGTCCTCGATCTgg   >  1:216433/1‑135 (MQ=255)                                                                                                                            tCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGGGTGGCAAGGATGGCCTGATCGACGGTGAGGTCGTCCGCGATCTgg   >  2:155860/1‑135 (MQ=255)                                                                                                                             cACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGGGTGGCAAGGATGGCCTGATCGACGGTGAGGTCGTCCTCGATCTGGt  <  2:216433/135‑1 (MQ=255)                                                                                                                             cACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGGGTGGCAAGGATGGCCTGATCGACGGTGAGGTCGTCCTCGATCTGGt  <  6:85804/135‑1 (MQ=255)                                                                                                                             cACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGGGTGGCAAGGATGGCCTGATCGACGGTGAGGTCGTCCTCGATCTGGt  <  3:290665/135‑1 (MQ=255)                                                                                                                               |                                                                                                                                     AGCACCACGCGGCGCCCGGTGTGCGGTTGGAAGATACGCTGCTGCTCGGCCGGTGACAGGCGCGCGTACAGCGGCAAAATCTCGGTATGGCGCAGTTGCGCCTTGCGCAGGATCTCGGCGGCATCACGGATTTCCCGCTCGCCCGGCAGGAAGATCAGCACATCGCCCGGGCCCTTGCCCTCGCTGCGTTCGTGCTGCGCCAGCTCGTCGAGGGTGGCAAGGATGGCCTGATCGACGGTGAGGTCGTCCTCGATCTGGT  >  ACB122/5160796‑5161054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑