Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,468,407 G→A 6.3% G221R (GGA→AGA)  CJ019_05146 → Putative 3‑methyladenine DNA glycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,468,4070GA 6.3% 183.8 / 2.6 63G221R (GGA→AGA) CJ019_05146Putative 3‑methyladenine DNA glycosylase
Reads supporting (aligned to +/- strand):  ref base G (25/34);  new base A (2/2);  total (27/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

GCCTGGGCATCCCGCACGGGCGCGACGAGCACCTGCCGTACCGCTTCGTCGATGCCGAATACGCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCTGCATCGAATGCCTGGCCATCGCCGGCATTATCGTGCCGGG  >  ACB122/5468273‑5468539
                                                                                                                                      |                                                                                                                                    
gCCTGGGCATCCCGCACGGGCGCGACGAGCACCTGCCGTACCGCTTCGTCGATTCCGAATACGCCCGTTTCTGCACACGGAACCCGTTAAGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAg                                                                                                                                      >  4:158162/1‑135 (MQ=255)
 ccTGGGCATCCCGCACGGGCGCGACGAGCACCTGCCGTACCGCTTCGTCGATGCCGAATACGCCCGTTTCTGCACCCGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAgg                                                                                                                                     >  8:158748/1‑135 (MQ=255)
 ccTGGGCATCCCGCACGGGCGCGACGAGCACCTGCCGTACCGCTTCGTCGATGCCGAATACGCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAACGCCGTGATTTCTTCATTCTCGAACAAg                                                                                                                                      >  4:279869/1‑134 (MQ=255)
             gCACGGGCGCGACGAGCACCTGCCGTACCGCTTCGTCGATGCCGAATACGCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATg                                                                                                                         >  7:294482/1‑135 (MQ=255)
             gCACGGGCGCGACGAGCACCTGCCGTACCGCTTCGTCGATGCCGAATACGCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCCAAATCGAAGGCCCTGATTTCTTCCTTCTCGACCAAGCAAGCTGAACATg                                                                                                                         >  7:2147/1‑135 (MQ=255)
              cACGGGCGCGACGAGCACCTGCCGTACCGCTTCGTCGATGCCGAATACGCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATgg                                                                                                                        <  2:191216/135‑1 (MQ=255)
               aCGGGCGCGACGAGCACCTGCCGTACCGCTTCGTCGATGCCGAATACGCCCGTTTCTGCACACGGACCCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATggg                                                                                                                       >  3:180654/1‑135 (MQ=255)
               aCGGGCGCGACGAGCACCTGCCGTACCGCTTCGTCGATGCCGAATACGCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATggg                                                                                                                       >  1:73568/1‑135 (MQ=255)
                  ggCGCGACGAGCACCTGCCGTACCGCTTCGTCGATGCCGAATACGCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCa                                                                                                                    >  7:62897/1‑135 (MQ=255)
                       gacgaGCACCTGCCGTACCGCTTCGTCGATGCCGAATACGCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGc                                                                                                               >  8:195057/1‑135 (MQ=255)
                         cgaGCACCTGCCGTACCGCTTCGTCGATGCCGAATACGCCCGTTTCTGCACACGGCACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTc                                                                                                             >  7:268049/1‑135 (MQ=255)
                                cTGCCGTACCGCTTCGTCGATGCCGAATACGCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCGAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGcc                                                                                                      <  7:70497/135‑1 (MQ=255)
                                cTGCCGTACCGCTTCGTCGATGCCGAATACGCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGcc                                                                                                      <  8:288518/135‑1 (MQ=255)
                                     gTACCGCTTCGTCGATGCCGAATACGCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGAcc                                                                                                 <  4:40978/135‑1 (MQ=255)
                                     gTACCGCTTCGTCGATGCCGAATACGCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGAcc                                                                                                 <  8:268049/135‑1 (MQ=255)
                                              cgtcgATGCCGAATACGCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGCACATGGGCCAATGGCTCGACAGCCTGACCggcaggc                                                                                          >  8:103737/1‑133 (MQ=255)
                                                tcgATGCCGAATACGCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAg                                                                                      <  4:175964/135‑1 (MQ=255)
                                                tcgATGCCGAATACGCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAg                                                                                      >  5:123333/1‑135 (MQ=255)
                                                 cgATGCCGAATACGCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTGATTCTCGCACAAGGCAGCTGAACATGGGCCAATGGCTCGACCGCCTGCCCGGCTGGCTCCgc                                                                                     >  5:189042/1‑135 (MQ=255)
                                                   aTGCCGAATACGCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAgcgc                                                                                   >  6:87999/1‑135 (MQ=255)
                                                    tGCCGAATACGCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGcc                                                                                  <  6:302066/135‑1 (MQ=255)
                                                        gAATACGCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAAcc                                                                              >  4:160289/1‑135 (MQ=255)
                                                         aaTACGCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAAccc                                                                             <  8:245613/135‑1 (MQ=255)
                                                         aaTACGCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAAccc                                                                             <  1:97967/135‑1 (MQ=255)
                                                         aaTACGCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAAccc                                                                             <  8:294482/135‑1 (MQ=255)
                                                              gCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGt                                                                        >  4:67377/1‑135 (MQ=255)
                                                              gCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGt                                                                        <  7:92683/135‑1 (MQ=255)
                                                                      cTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTgg                                                                 <  5:171494/134‑1 (MQ=255)
                                                                      cTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGc                                                                >  6:83121/1‑135 (MQ=255)
                                                                          acacGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTg                                                             <  2:90054/134‑1 (MQ=255)
                                                                                 aCCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAAGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGAtttt                                                     >  7:183474/1‑135 (MQ=255)
                                                                                 aCCCGTTACGTCGCGGCCAAGTCGAAGGCCGTCATTTCTTCATTCTCGAACAAAGAAGCTGAACATGCGCCAATCGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCCCAGTGGCTTGGCCTGGCGAgttt                                                     >  7:178869/1‑135 (MQ=255)
                                                                                  cccGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTc                                                    <  8:170430/135‑1 (MQ=255)
                                                                                  cccGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTc                                                    <  4:26448/135‑1 (MQ=255)
                                                                                   ccGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATGTTcc                                                   >  7:209883/1‑135 (MQ=255)
                                                                                       tACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGACGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCtggt                                               <  8:261420/135‑1 (MQ=255)
                                                                                             gcgGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCt                                          >  1:34063/1‑134 (MQ=255)
                                                                                                gCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGCACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCTGCAt                                      <  5:158774/135‑1 (MQ=255)
                                                                                                 ccAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCTGCAt                                      >  4:268734/1‑134 (MQ=255)
                                                                                                 ccAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCTGCAt                                      >  3:73288/1‑134 (MQ=255)
                                                                                                 ccAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCAGGCGATTTTCCTGGTGGCCTGCAt                                      >  2:201126/1‑134 (MQ=255)
                                                                                                         aaGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCG‑CAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCTGCATCGAATGCCTg                            <  8:286656/135‑1 (MQ=255)
                                                                                                            gCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCTGCATCGAATGCCTgg                           >  7:248268/1‑134 (MQ=255)
                                                                                                               gTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCTGCATCGAATGCCTGGCCAt                       <  8:91397/135‑1 (MQ=255)
                                                                                                               gTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCTGCATCGAATGCCTGGCCAt                       <  1:59039/135‑1 (MQ=255)
                                                                                                               gTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCTGCATCGAATGCCTGGCCAt                       <  8:62897/135‑1 (MQ=255)
                                                                                                               gTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCTGCATCGAATGCCTGGCCAt                       <  3:96624/135‑1 (MQ=255)
                                                                                                                   tttCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCTGCATCGAATGCCTGGCCATCGcc                   >  7:295493/1‑135 (MQ=255)
                                                                                                                   tttCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCTGCATCGAATGCCTGGCCATCGc                    <  3:8542/134‑1 (MQ=255)
                                                                                                                   tttCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTACTGGTGGCCTGCATCGAATTCATGGCCATCGcc                   >  2:46458/1‑135 (MQ=255)
                                                                                                                   tttCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCAGGCGATTTTCCTGGTTGCCTGCATCGAAATCCTGGCCATCGcc                   >  6:233856/1‑135 (MQ=255)
                                                                                                                   tttCTTCATTATCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCCACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCTTCATCGAAAGCCAGGCCATCGcc                   >  3:275501/1‑135 (MQ=255)
                                                                                                                          aTTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCTGCATCGAATGCCTGGCCATCGCCGGCAtt             <  8:248268/134‑1 (MQ=255)
                                                                                                                                 taCAAAGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCTGCATCGAATGCCTGGCCATCGCCGGCATTATCGTGcc     <  8:178869/134‑1 (MQ=255)
                                                                                                                                 aaCAAGGAAGCTGAACCTGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCTGCATCGAATGCCTGGCCATCGCCGGCATTATCGTGcc     <  8:118004/135‑1 (MQ=255)
                                                                                                                                 aaCAAGGAAGCTGAACATGGGCCTATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCGTGGTGGCCTTCATCGAATGCCTGGCCATCGCCGGCATTATCTTGcc     <  1:201126/135‑1 (MQ=255)
                                                                                                                                 aaCAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCTGCATCGAATGCCTGGCCATCGCCGGCATTATCGTGcc     <  7:195057/135‑1 (MQ=255)
                                                                                                                                 aaCAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCTGCATCGAATGCCTGGCCATCGCCGGCATTATCGTGcc     <  3:48803/135‑1 (MQ=255)
                                                                                                                                 aaCAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCTGCATCGAATGCCTGGCCATCGCCGGCATTATCGTGcc     <  3:222046/135‑1 (MQ=255)
                                                                                                                                 aaCAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGGGGCCTGCATCGAATGCCTGGCCATCGCCGGCATTATCGt        <  3:279869/132‑1 (MQ=255)
                                                                                                                                 aaCAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCGTGGCCTGGCGATTTTCCTGGTGGCCTGCATCGAATGCCTGGCCATCGCCGGCATTATCGTGcc     <  1:106243/135‑1 (MQ=255)
                                                                                                                                 aaCAAAGAAGCTGAACATGGGCCAATTGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCTGCATCGAATGCCTGTCCATCGCCGGCATTATCGTGcc     <  8:183474/135‑1 (MQ=255)
                                                                                                                                   cAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCTGCATCGAATGCCTGGCCATCGCCGGCATTATCGTGCCgg   <  6:265382/135‑1 (MQ=255)
                                                                                                                                   cAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCTGCATCGAATGCCTGGCCATCGCCGGCATTATCGTGCCgg   <  3:194602/135‑1 (MQ=255)
                                                                                                                                   cAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCTGCATCGAATGCCTGGCCATCGCCGGCATTATCGTGCCgg   <  8:295493/135‑1 (MQ=255)
                                                                                                                                    aaGGAAGCTGAACATCGGCCAATGGCTCGACAGCCTGACCGGCTCCCTCAGCGCCAACCCGCAGTGGCATGGCCTGGCGATTTTCCTGCAGGCCAGCATCGAAAGCCAGGCCAACGCCCGCATTATCGTGCCcgg  >  3:156625/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                    
GCCTGGGCATCCCGCACGGGCGCGACGAGCACCTGCCGTACCGCTTCGTCGATGCCGAATACGCCCGTTTCTGCACACGGAACCCGTTACGTCGCGGCCAAGTCGAAGGCCGTGATTTCTTCATTCTCGAACAAGGAAGCTGAACATGGGCCAATGGCTCGACAGCCTGACCGGCTGGCTCAGCGCCAACCCGCAGTGGCTTGGCCTGGCGATTTTCCTGGTGGCCTGCATCGAATGCCTGGCCATCGCCGGCATTATCGTGCCGGG  >  ACB122/5468273‑5468539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑