Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,688,645 T→G 46.6% R238R (CGT→CGG)  pyrC' → Dihydroorotase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,688,6450TG46.6% 27.4 / 9.4 45R238R (CGT→CGG) pyrC'Dihydroorotase‑like protein
Reads supporting (aligned to +/- strand):  ref base T (11/11);  new base G (3/19);  total (15/30)
Fisher's exact test for biased strand distribution p-value = 2.17e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCT  >  ACB122/5688513‑5688776
                                                                                                                                    |                                                                                                                                   
aGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCCCCCCCGCCCGTgg                                                                                                                                   >  2:56449/1‑135 (MQ=255)
        tGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGGGCGGCCGTCCGCCACATCACCCGCCCCCCAGGCGCCCGGc                                                                                                                           >  1:88799/1‑135 (MQ=255)
                  ccTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGGGCGGGGGCCCGTCAGCCCCCTCACCCCCGCCCCGGGCGCGCGGGGGCTCgcccc                                                                                                                 >  7:34190/1‑134 (MQ=255)
                   cgcggccggccggcCAGCCCGCCGCCCGCCTAAACGGGGGCCCGGGCGCGCAACCTGCGGGGGGTGCAACAGCGCGGCGGGCGGGCGCACTTCAGCATGACCCCCCGCGCCCGGGGCGCGCGGCTGATCgcccag                                                                                                                <  4:87631/133‑1 (MQ=255)
                   cgcggccggcGGGCCAGCCGGTAGAGCGCCGAAACCGGGGCCGGGGGGCGCACCCTGGTGGGGGGGCACCAGCGCGGCGTGGGTGCGCACTCCCGCCTCAACCCCCGCGCCCGGGGCGCGCGGCTGATCgcccag                                                                                                                <  4:109173/133‑1 (MQ=255)
                       gCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTGAGCCCGATCACCCGCGCCCGGGGCGCGCGGCGGGTCgccccggccc                                                                                                            >  8:191776/1‑135 (MQ=255)
                               ggccgcccGGCGAGCGCCGAACCGGGGCCCCGGGCGCGCACCGTGGTGCGGGGGTACCCGCGGGGCGGGCGGGCGCAGTCCCGCCAGAGCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTg                                                                                                    <  2:103506/130‑1 (MQ=255)
                                          gagCGCCGAAACCGTGGCCCGGGCGCGCAACCGGGTGCGGGGGGAACAGAGCGGGGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGt                                                                                         <  8:205090/135‑1 (MQ=255)
                                             cgcgGAACCCGGGGCCGTGGCGCGCAACCTGCTGGGGGTGTAACTGAGCGGGGTGCGGGCGCAGTGCAGCCAGTTCACCAGGGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                      <  6:140821/135‑1 (MQ=255)
                                             cgcCTAAACCGTGGCCGTGGCGCGCAACCTGGTGCTGGTGGAACATAGGGGCGTGCGTGCGCACTTCAGCATGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGt                                                                                         <  8:205089/132‑1 (MQ=255)
                                             ccccGAACCCGGGGCCGGGGGGCGCAACGTGCTGCTGGGGCAACTGCGGGGGGGGCGGGGGCACTGCCCCCACAGCCCCGGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                      <  2:78232/133‑1 (MQ=255)
                                               ccGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGGGGACCAGAGCGGCGTGCGTGCGCACTTCCGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccg                                                                                    <  4:143303/135‑1 (MQ=255)
                                                cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGGTGGGACTTGAGCCCGCGACCCCACGCCCCTGGCGCGCGGCGGGTCGCCCCGGCCCCGGGCGTGGGGCGGGCGGtttacgc                                                                                   >  2:231691/1‑129 (MQ=255)
                                                cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGGGGGCCCATACGCCCCATCACCCCCCCCCCGGGGGCGCCGCTGCTAGCCCACGCCCCCGCCCTGGGCCGGCCCGTGAccgc                                                                                   >  8:41911/1‑135 (MQ=255)
                                                cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCACTTGAGCCCGATAACCCGCGCCCCTGGCGCGCCGCGGATCGCCCCCGCCCCCGCCGTGGGCCTGCCGGGAAccgc                                                                                   >  2:198935/1‑135 (MQ=255)
                                                cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCACTTCCGCCAGATCACCCGCGCCCGTGGGGCGCGGCGGCTCGCCCCGGCCCCGGCCGGGGGCCTGGCGGTAAccgc                                                                                   >  6:148736/1‑135 (MQ=255)
                                                     cgggggCCCGGGCGCGCAACCTGCTGCTGGTGCAACAGAGCGGCGTGCGGGCGCACTTCAGCCAGACCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATg                                                                              <  7:199312/131‑1 (MQ=255)
                                                       ggggCCGTGGCGCTCACCCTGCGGGTGGGGCAACAGGGGGGCGGGCGGGCGCACTACCCCCAGAACCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                            <  8:117575/133‑1 (MQ=255)
                                                       ggggCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGGGGCGGGCGTGCGCACTTCAGCCAGATCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                            <  7:191776/133‑1 (MQ=255)
                                                       ggggCCCGGGCGCGAACCCTGCGGGGGGGGCAACAGAGCGGCGGGCGGGCGCACTACCGCCAGAGCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                            <  8:91759/133‑1 (MQ=255)
                                                       gcggCCCGGGCCGGCAACCTCTTGGTGGTGAAACCTTGCGGGGTGCTTCCGCACTACAGCCTGATCCACCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                            <  7:238635/133‑1 (MQ=255)
                                                       gTGGCCCTGGGGCGCAACCTGCTGGTGTTGCCACTGAGCGGCGTGCGGGCGAAATTCTGCAAGATCACCAGCGCGCGTGGCGCGCGGCTGATCGCCCAGGCCCAGTCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                            <  8:87698/135‑1 (MQ=255)
                                                       gTGGCCCGGGCGCGCAACCTGTTGCTGGTGGAACAGAGCGGGTGGCGTGGTCAGTTCAGCCAGATAACGAGCGCCCGTGGAGCGCGGCTGATCGCCGAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                            <  2:85170/135‑1 (MQ=255)
                                                           cccGGGCGCTCACCGGGCTGCGGGGGCAACAGCGCGGGGGGGGGGCGCACTCCACCCATACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAgcgc                                                                        <  3:253510/135‑1 (MQ=255)
                                                                  gcgcAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCCGCTGATCGCCCACGCCCCGGCCTTGGGCCTGCCGGGAACCGCCGATGTAGCGCGGTACCa                                                                 >  7:75481/1‑135 (MQ=255)
                                                                                           cgcggggGGCGTGCGCACTGCGGCCCGACCCCCCGGGCGGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTg                                        <  7:152514/134‑1 (MQ=255)
                                                                                             ggcggggcgtgcgAAATCCAGCCCTAACCCCTGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                                      <  6:4533/129‑1 (MQ=255)
                                                                                             cggcggGCGGGCGCACTCCAGCCAGAGCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                                      <  8:172778/135‑1 (MQ=255)
                                                                                               gcgggcggGCGCACTCCCCCCAGACCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGcgcg                                    <  8:17566/135‑1 (MQ=255)
                                                                                                  ggcgtgcgCACTTCTGCCACAGCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                 <  2:88799/134‑1 (MQ=255)
                                                                                                  ggcgtgcgCACTTCAGCCAGAACCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                 <  3:60583/134‑1 (MQ=255)
                                                                                                   gcgtgcgCACTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGtt                                <  2:15784/135‑1 (MQ=255)
                                                                                                       gcgcACTGCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc                            <  4:73248/135‑1 (MQ=255)
                                                                                                             ttCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGCGTGCTCCAGCCt                       >  6:206565/1‑134 (MQ=255)
                                                                                                                aGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTAc                   >  6:244996/1‑135 (MQ=255)
                                                                                                                     gATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGc                                          >  8:209728/1‑107 (MQ=255)
                                                                                                                     gATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGc                                          <  7:209728/107‑1 (MQ=255)
                                                                                                                         ccccgcgcCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAg          <  5:268556/131‑1 (MQ=255)
                                                                                                                         aCCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTATCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAg          >  8:76203/1‑135 (MQ=255)
                                                                                                                          ccAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAgc         >  6:142660/1‑135 (MQ=255)
                                                                                                                               gcCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGccgccg    <  1:198935/135‑1 (MQ=255)
                                                                                                                                 ccGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCCCGTGCAGCCGCCGCt  >  4:239946/1‑135 (MQ=255)
                                                                                                                                 ccGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCt  <  5:244996/135‑1 (MQ=255)
                                                                                                                                 ccGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGAGGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCGGCGCGAGTTCTCCAGCCTGTCCCACGGGCAGCCGCCGCt  <  3:108293/135‑1 (MQ=255)
                                                                                                                                 ccGGGGCCCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCt  <  4:289981/135‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                   
AGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCT  >  ACB122/5688513‑5688776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑