Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	5,806,024	G→T	13.8%	A97S (GCC→TCC)	argS →	Arginine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	5,806,024	0	G	T	13.8%	78.3 / 4.1	29	A97S (GCC→TCC)	argS	Arginine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (14/11);  new base T (2/2);  total (16/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGGCCAACCGCCTGGATGCCGCCCTGGCCGACGCCCACCTGGGTGCGCGCAAAGCCGGCCCGGCGCAAAAGGTGGTGATCGACATGTCGGCACCCAACCTGGCCAAGGAAATGCACGTCGGCCACCTGCG  >  ACB122/5805892‑5806151                                                                                                                                     |                                                                                                                                aaGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGGc                                                                                                                                >  2:299834/1‑134 (MQ=255)      ggccggcATGAAGCCGCGCGCCCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGCCATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGGCCACCCg                                                                                                                          >  2:33188/1‑135 (MQ=255)      ggccggcATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGCCATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCCGACCACCGACGCCCTGGCCACCCg                                                                                                                          >  1:30576/1‑135 (MQ=255)           gcATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCACGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCGTCCAGAACACCGCCGCCCTGGCCAACCGCCTgg                                                                                                                     >  6:62326/1‑135 (MQ=255)           gcATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGGCCAACCGCCTgg                                                                                                                     <  1:171775/135‑1 (MQ=255)                         gACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGCCGCCCTGTCCAACCGCCTGGATGCCGCCCTGGcc                                                                                                       >  1:282654/1‑135 (MQ=255)                         gACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGTCCACCCGCCTGGATGCCGCCCTGGcc                                                                                                       >  1:286282/1‑135 (MQ=255)                         gACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCCTCCAGAACACCGACGCCCTGGCCAACCGCCTGGATGCCGCCCTGGcc                                                                                                       <  6:241892/135‑1 (MQ=255)                         gACCTCGCCGAAAAACTGAGCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGGCCAACCGCCTGGATGCCGCCCTGGcc                                                                                                       <  7:29790/135‑1 (MQ=255)                                             cAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGCACACCGACGCCCTGGCCACCCGCCTGGATGCCGCCCTGGCCGACGCCCACCTGGGTgcgcg                                                                                   >  5:44661/1‑135 (MQ=255)                                             cAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGGCCAACCGCCTGGATGCCGCCCTGGCCGACGCCCACCTGGGTgcgc                                                                                    >  6:108699/1‑134 (MQ=255)                                              aaCGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGGCCAACCGCCTGGATGCCGCCCTGGCCGACGCCCACCTg                                                                                           >  6:224655/1‑126 (MQ=255)                                              aaCGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGGCCAACCGCCTGGATGCCGCCCTGGCCGACGCCCACCTg                                                                                           <  5:224655/126‑1 (MQ=255)                                              aaCGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGGCCAACCGCCTGGATGCCGCCCTGGCCGACGCCCACCTGGGTgcgcg                                                                                   >  4:263222/1‑134 (MQ=255)                                                        cGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGGCCAACCGCCTGGATGCCGCCCTGGCCGACGCCCACCTGGGTGCGCGCAAAGccgg                                                                          <  1:58476/133‑1 (MQ=255)                                                              gcgcCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGGCCAACCGCCTGGATGCCGCCCTGGCCGACGCCCACCTGGGTGCGCGCAAAGCCGGCCCGgcgc                                                                  <  4:81188/135‑1 (MQ=255)                                                                  cGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGGCCAACCGCCTGGATGCCGCCCTGGCCGACGCCCACCTGGGTGCGCGCAAAGCCGGCCCGGCGCaaaa                                                              >  1:64618/1‑135 (MQ=255)                                                                  cGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGGCCAACCGCCTGGATGCCGCCCTGGCCGACGCCCACCTGGGTGCGCGCAAAGCCGGCCCGGCGCaaaa                                                              >  4:62672/1‑135 (MQ=255)                                                                                  gagaTTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGGCCAACCGCCTGGATGCCGCCCTGGCCGACGCCCACCTGGGTGCGCGCAAAGCCGGCCCGGCGCAAAAGGTGGTGATCGACATg                                              >  6:42661/1‑135 (MQ=255)                                                                                           ggCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGTCCAACCGCCTGGATGCCGCCCTGGCCGACGCCCACCTGGGTGCGCGCAAAGCCGGCCCGGCGCAAAAGGTGGTGATCGACATGTCGGCAccc                                     <  2:282654/135‑1 (MQ=255)                                                                                           ggCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGTCCAACCGCCTGGATGCCGCCCTGGCCGACGCCCACCTGGGTGCGCGCAAAGCCGGCCCGGCGCAAAAGGTGGTGATCGACATGTCGGCAccc                                     <  2:286282/135‑1 (MQ=255)                                                                                           ggCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGGCCAACCGCCTGGATGCCGCCCTGGCCGACGCCCACCTGGGTGCGCGCAAAGCCGGCCCGGCGCAAAAGGTGGTGATCGACATGTCGGCAccc                                     <  5:108699/135‑1 (MQ=255)                                                                                           ggCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGGCCAACCGCCTGGATGCCGCCCTGGCCGACGCCCACCTGGGTGCGCGCAAAGCCGGCCCGGCGCAAAAGGTGGTGATCGACATGTCGGCAccc                                     <  3:88490/135‑1 (MQ=255)                                                                                            gCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGGCCAACCGCCTGGATGCCGCCCTGGCCGACGCCCACCTGGGTGCGCGCAAAGCCGGCCCGGCGCAAAAGGTGGTGATCGACATGTCGGCACCCa                                    >  1:89403/1‑135 (MQ=255)                                                                                             ccccGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGGCCAACCGCCTGGATGCCGCCCTGGCCGACGCCCACCTGGGTGCGCGCAAAGCCGGCCCGGCGCAAAAGGTGGTGATCGACATGTCGGCACCCaa                                   >  3:297103/1‑135 (MQ=255)                                                                                                 ggCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGGCCAACCGCCTGGATGCCGCCCTGGCCGACGCCCACCTGGGTGCGCGCAAAGCCGGCCCGGCGCAAAAGGTGGTGATCGACATGTCGGCACCCAACCTg                               <  7:12642/135‑1 (MQ=255)                                                                                                  gCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGGCCAACCGCCTGGATGCCGCCCTGGCCGACGCCCACCTGGGTGCGCGCAAAGCCGGCCCGGCGCAAAAGGTGGTGATCGACATGTCGGCACCCAACCTgg                              <  4:297103/135‑1 (MQ=255)                                                                                                                             aCGCCCTGGCCAACCGCCTGGATGCCGCCCTGGCCGACGCCCACCTGGGTGCGCGCAAAGCCGGCCCGGCGCAAAAGGTGGTGATCGACATGTCGGCACCCAACCTGGCCAAGGAAATGCACGTCGGCCACCTgc   <  1:299834/135‑1 (MQ=255)                                                                                                                              cGCCCTGGCCAACCGCCTGGATGCCGCCCTGGCCGACGCCCACCTGGGTGCGCGCAAAGCCGGCCCGGCGCAAAAGGTGGTGATCGACATGTCGGCACCCAACCTGGCCAAGGAAATGCACGTCGGCCACCTgcg  >  6:78077/1‑135 (MQ=255)                                                                                                                                     |                                                                                                                                AAGCCGGCCGGCATGAAGCCGCGCGACCTCGCCGAAAAACTGATCAACGCCCTGCCGGCCAGCGCCGACATCAGCAAGGTCGAGATTGCAGGCCCCGGCTTCCTCAACTTCTTCCAGAACACCGACGCCCTGGCCAACCGCCTGGATGCCGCCCTGGCCGACGCCCACCTGGGTGCGCGCAAAGCCGGCCCGGCGCAAAAGGTGGTGATCGACATGTCGGCACCCAACCTGGCCAAGGAAATGCACGTCGGCCACCTGCG  >  ACB122/5805892‑5806151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑