Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	763,776	T→G	28.2%	V137G (GTC→GGC)	thrC_1 →	Threonine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	763,776	0	T	G	28.2%	51.9 / 5.0	39	V137G (GTC→GGC)	thrC_1	Threonine synthase
Reads supporting (aligned to +/- strand):  ref base T (11/16);  new base G (2/10);  total (13/26)
Fisher's exact test for biased strand distribution p-value = 2.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTGGCCATAACGAATGGATCCTGCAGCTTTTCCATGGGCCTACCCGGTCCTCCAAGGACTTTGCCGCGCAGTTGCAGGCGCGCTTTGTCCGGCACTTTCTGGGGGCAGAGTGCGAGCGGGTCATGCTGGTCGGTGCCAGCAACGGCGATACCGCAGTAGCGGCTATCGAAGCGTTGCGGCATTGCCCTGCAGCAGGCTTGCTGGCGCTATACCCCAGCGCAGGCACGCCCGCCGACCGCGCGGCAGTGATGCGCAGTG  >  ACB122/763645‑763904                                                                                                                                    |                                                                                                                                 gcTTGGCCATAACGAATGGATCCTGCAGCTTTTCCATGGGCCTACCCGGTCCTCCAAGGACTTTGCCGCGCAGTTGCAGGCGCGCTTTGTCCGGCACTTTCTGGGGGCAGAGTGCGAGCGGGTCATGCTGGTCgg                                                                                                                               <  4:234811/135‑1 (MQ=255)      gCCATAACGAATGGATCCTGCAGCTTTTCCATGGGCCTACCCGGTCCTCCAAGGACTTTGCCGCGCAGTTGCAGGCGCGCTTTGTCCGGCACTTTCTGGGGGCAGAGTGCGAGCGGGTCATGCTGGTCGGTGcc                                                                                                                           <  5:136154/134‑1 (MQ=255)           aaCGAATGGATCCTGCAGCTTTTCCATGGGCCTACCCGGTCCTCCAAGGACTTTGCCGCGCAGTTGCAGGCGCGCTTTGTCCGGCACTTTCTGGGGGCAGAGTGCGAGCGGGTCATGCTGGTCGGGGCCAGCAAc                                                                                                                     <  1:213591/135‑1 (MQ=255)              gAATGGATCCTGCAGCTTTTCCATGGGCCTACCCGGTCCTCCAAGGACTTTGCCGCGCAGTTGCAGGCGCGCTTTGTCCGGCACTTTCTGGGGGCAGAGTGCGAGCGGGTCATGCTGGTCGGTGCCAGCAACgg                                                                                                                   >  1:181916/1‑134 (MQ=255)              gAATGGATCCTGCAGCTTTTCCATGGGCCTACCCGGTCCTCCAAGGACTTTGCCGCGCAGTTGCAGGCGCGCGTTGTCCGGCACTTTCTGGGGGCAGAGTGCGCGCGGGTCATGCTGGTCGGTGCCCCCAACGGc                                                                                                                  >  7:111816/1‑135 (MQ=255)                         gCAGCTTTTCCATGGGCCTACCCGGTCCTCCAAGGACTTTGCCGCGCAGTTGCAGGCGCGCGTTGTCCGGCACGTTATTGGGGCAGAGTGCGAGCGGGGCCAGCGGTGGGGTGCCCGCCACCGCCATACCGCa                                                                                                         >  5:183781/1‑133 (MQ=255)                           aGCTTTTCCATGGGCCTACCCGGTCCTCCAAGGACTTTGCCGCGCAGTTGCAGGCGCGCTTTGTCCGGCACTTTCTGGGGGCATAGTGCGAGCGGGTCATGCTGGTCGGTGCCAGCAACGGCGATACCGCAGt                                                                                                       <  8:92918/133‑1 (MQ=255)                           aGCTTTTCCATGGGCCTACCCGGTCCTCCAAGGACTTTGCCGCGCAGTTGCAGGCGCGCTTTGTCCGGCACTTTCTGGGGGCAGAGTGCGAGCGGGTCATGCTGGTCGGTGCCAGAAACGGCGATACCGCAGTAg                                                                                                     <  2:181916/135‑1 (MQ=255)                            gCTTTTCCATGGGCCTACCCGGTCCTCCAAGGACTTTGCCGCGCAGTTGCAGGCGCGCTTTGTCCGGCACTTTCTGGGGGCAGAGTGCGAGCGGGTCATGCTGGTCGGTGCCAGCAACGGCGATACCGCAGTAGc                                                                                                    >  3:18094/1‑135 (MQ=255)                                         ccTACCCGTTCCTCCAAGGACTTTGCCGCGCAGTTGCAGGCGCGCTTTGTCCGGCACTTTCTGGGGGCAGAGTGCGAGCGGGTCTTGATGGTCGGTGCCTGCTACGTCGATACCGCAGTAGCGGCTATCTAAGCg                                                                                       <  6:255751/135‑1 (MQ=255)                                         ccTACCCGGTCCTCCAAGGACTTGGCCGCGCTGTTGCTGGCGCGCTTTGTCCGGCACTTTCTGGGGGCAGTGTGCGTGGGGGTCTTGCTGGTCGGTGCAACAAACGGCGATACCGCAGTAGCGGCTATCGAAGCg                                                                                       <  5:164459/135‑1 (MQ=255)                                              ccGGTCCTCCAAGGACTTTGCCGCGCAGTTGCAGGCGCGCTTTGTCCGGCACTTTCTGGGGGCAGAGTGCGAGCGGGTCATGCTGGTCGGTGCCAGCAACGGCGATACCGCAGTAGCGGCTATCGAAGCGTTg                                                                                    <  7:56853/133‑1 (MQ=255)                                              ccGGACCCCCAAGCACTTTGCCCCGCAGTTGCAGGCGCGCTTTGTCCGGCACTTTCTGGGGGCAGAGTGCGAGCGGGTCATGCTGGTCGGGGCCAGCAACGGCGATACCGCAGTAGCGGCTAGCGAAGCGTTGCg                                                                                  <  6:53030/135‑1 (MQ=255)                                                     tccAAGGACTTTGCCGCGCAGTTGCAGGCGCGCTTTGTCCGGCACTTTCTGGGGGCAGAGTGCGAGCGGGTCATGCTGGTCGGTGCCAGc                                                                                                                        >  8:218655/1‑90 (MQ=255)                                                     tccAAGGACTTTGCCGCGCAGTTGCAGGCGCGCTTTGTCCGGCACTTTCTGGGGGCAGAGTGCGAGCGGGTCATGCTGGTCGGTGCCAGc                                                                                                                        <  7:218655/90‑1 (MQ=255)                                                     tccAAGGACTTTGCCGCGCAGTTGCAGGAGCGCTTTGTCCGGCACTTTCTGGGGCAAGAGTCCGCCCGGGTCATCCTGGTCGGTCCCAGCAACGGCGATACCCCATTAGCGGCTATCGAAGCGTTCCGCCATTGc                                                                           >  8:236419/1‑135 (MQ=255)                                                             cTTTGCCGCGCAGTTGCAGGCGCGCTTTGTCCGGCACTTTCTGGGGGCAGAGTGCGAGCGGGTCATGCGGGGCGGTGCCAGCAACGGCGATACCGCAGTAGCGGCTATCGAAGCGTTGCGGCATTGCCCTgcagc                                                                   <  7:148025/135‑1 (MQ=255)                                                                  ccGCGCAGTTGCAGGCGCGCTTTGGCCGGCACTTTCTGGGGGCAGAGTGCGAGCGGGTCATGCTGGGCGGTGCCAGCAACGGCGATACCGCAGTAGCGGCTATCGAAGCGTTGCGGCATTGCCCTGCAGCAGGCt                                                              <  6:183781/135‑1 (MQ=255)                                                                   cgcgCAGTTGCAGGCGCGGTTTGTCCGGCACTTTCTGGGGGCAGAGTGCGAGCGGGTCATGCTGGTCGGTGCCAGCAACGGCGATACCGCAGTAGCGGATATCGAAGCGTTGCGGCATTGCCCTGCAGCAGGCtt                                                             >  7:173152/1‑135 (MQ=255)                                                                   cgcgCAGTTGCAGGCGCGCTTTGTCCGGCACTTTCTGGGGGCAGAGTGCGAGCGGGTCATGCTGGGCGGTGCCAGCAACGGCGATACCGCAGTAGCGGCTATCGAAGCGTTGCGGCATTGCCCTGCAGCAGGCtt                                                             >  3:78969/1‑135 (MQ=255)                                                                     cgcAGTTGCAGGCGCGCTTTGTCCGGCACTTTCTGGGGGCAGAGTGCGAGCGGGTCATGCTGGTCGGTGCCAGCAACGGCGATACCGCAGTAGCGGCTATCGAAGCGTTGCGGCATTGCCCTGCAGCAGGCTTGc                                                           <  3:90465/135‑1 (MQ=255)                                                                       cAGTTGCAGGCGCGCTTTGTCCGGCACTTTCTGGGGGCAGAGTGCGAGCGGGTCATGCTGGGCGGTGCCAGCAACGGCGATACCGCAGTAGCGGCTATCGAAGCGTTGCGGCATTGCCCTGCAGCAGGCTTGCTg                                                         <  7:154476/135‑1 (MQ=255)                                                                          tggCAGGCGCGCTTGGTCCGGCACTTTCTGGGGGCAGAGTGCGCGCGGGTCATGCGGGGCGGTGCCAGCAACGGCGATACCGCAGTAGCGGCTATCGAAGCGTTGCGGCATTGCCCTGCAGCAGGCTTGCTGgcg                                                      <  7:118441/133‑1 (MQ=255)                                                                               ggCGCGCTTTGTCCGGCACTTTCTGGGGGCAGAGTGCGAGCGGGTCATGCTGGGCGGTGCCAGCAACGGCGATACCGCAGTAGCGGCTATCGAAGCGTTGCGGCATTGCCCTGCAGCAGGCTTGCTGGCGCtata                                                 <  6:96982/135‑1 (MQ=255)                                                                                       ttGTCCGGCACTTTCTGGGGGCAGAGTGCGAGCGGGTCATGCTGGTCGGTGCCAGCAACGGCGATACCGCAGTAGCGGCTATCGAAGCGTTGCGGCATTGCCCTGCAGCAGGCTTGCTGGCGCTATACCCCAgcg                                         >  1:56065/1‑135 (MQ=255)                                                                                          gccGGCACTTTCTGGGGGCCGAGGCCGCGCGGGTCAGGCTGGGCGGGGCCAGCAACGGCGATACCGCAGTAGCGGCTACCGAAGCGTTGCGGCATTGCCCTGCAGCAGGCTTGCTGGCGCTATACCCCAGCGCAg                                      <  5:192909/134‑1 (MQ=255)                                                                                                  ttccTGGGGGCAGAGGGCGAGCGGGTCCTGCTGGGCGGGGCCAGCAACGGCGATACCGCAGTAGCGGCTATCGAAGCGTTGCGGCATTGCCCTGCAGCAGGCTTGCTGGCGCTATACCCCAGCGCAGGCAcgccc                              <  5:4092/132‑1 (MQ=255)                                                                                                       gggggCAGAGTGCTCGCGGGGCATGCTGGGCGGTGCCAGCAACGGCGATACCGCAGTAGCGGCTATCGAAGCGTTGCGGCATTGCCCTGCAGCAGGCTTGCTGGCGCTATACCCCAGCGCAGGCACGCCCGCCGa                         <  1:104495/135‑1 (MQ=255)                                                                                                         gggCAGAGTGCGAGCGGGTCATGCGGGGCGGGGCCAGCAACGGCGATACCGCAGTAGCGGCTATCGAAGCGTTGCGGCATTGCCCTGCAGCAGGCTTGCTGGCGCTATACCCCAGCGCAGGCACGCCCGCCGa                         <  5:87900/133‑1 (MQ=255)                                                                                                            cAGAGTGCGCGCGGGTCATGCTGGTCGGGCCCAGCAACGGCGATACCGCAGTAGCGGCTATCGAAGCGTTGCGGCATTGCCCTGCAGCAGGCTTGCTGGCGCTATACCCCAGCGCAGGCACGCCCGCCGACcgcg                    <  8:115578/135‑1 (MQ=255)                                                                                                            cAGAGTGCGAGCGGGTCATGCTGGGCGGTGCCAGCAACGGCGATACCGCAGTAGCGGCTATCGAAGCGTTGCGGCATTGCCCTGCAGCAGGCTTGCTGGCGCTATACCCCAGCGCAGGCACGCCCGCCGACcgcg                    <  4:78969/135‑1 (MQ=255)                                                                                                              gagGGCGAGCGGGTCATGGTGGTCGGGGCCAGCAACGGCGATACCGCAGTAGCGGCTATCGAAGCGTTGCGGCATTGCCCTGCAGCAGGCTTGCTGGCGCTATACCCCAGCGCAGGCACGCCCGCCGACcgcgcg                  <  8:111816/135‑1 (MQ=255)                                                                                                                 tGCGAGCGGGTCATGCTGGTCGGTGCCAGCAACGGCGATACCGCAGTAGCGGCTATCGAAGCGTTGCGGCATTGCCCTGCAGCAGGCTTGCTGGCGCTATACCCCAGCGCAGGCACGCCCGCCGACCGCGCGGCa               <  4:18094/135‑1 (MQ=255)                                                                                                                      gCGGGTCATGCTGGTCGGTGCCAGCAACGGCGATACCGCAGTAGCGGCTATCGAAGCGTTGCGGCATTGCCCTGCAGCAGGCTTGCTGGCGCTATACCCCAGCGCAGGCACGCCCGCCGACCGCGCGGCAGTGAt          <  7:236419/135‑1 (MQ=255)                                                                                                                           tCATGCTGGTCGGTGCCAGCAACGGCGATACCGCAGTAGCGGCTATCGAAGCGTTGCGGCATTGCCCTGCAGCAGGCTTGCTGGCGCTATACCCCAGCGCAGGCACGCCCGCCGACCGCGCGGCAGTGATGCGCa     <  2:177105/135‑1 (MQ=255)                                                                                                                            cATGCTGGTCGGTGCCAGCAACGGCGATACCGCAGTAGCGGCTATCGAAGCGTTGCGGCATTGCCCTGCAGCAGGCTTGCTGGCGCTATACCCCAGCGCAGGCACGCCCGCCGACCGCGCGGCAGTGATGCGCAg    >  3:104990/1‑135 (MQ=255)                                                                                                                              tGCTGGTCGGTGCCAGCAACGGCGATACCGCAGTAGCGGCTATCGAAGCGTTGCGGCATTGCCCTGCAGCAGGCTTGCTGGCGCTATACCCCAGCGCAGGCACGCCCGCCGACCGCGCGGCAGTGATGCGCAGTg  >  5:129996/1‑135 (MQ=255)                                                                                                                              tGCTGGTCGGTGCCAGCAACGGCGATACCGCAGTAGCGGCTATCGAAGCGTTGCGGCATTGCCCTGCAGCAGGCTTGCTGGCGCTATACCCCAGCGCAGGCACGCCCGCCGACCGCGCGGCAGTGATGCGCAGTg  >  6:249217/1‑135 (MQ=255)                                                                                                                              tGCTGGTCGGTGCCAGCAACGGCGATACCGCAGTAGCGGCTATCGAAGCGTTGCGGCATTGCCCTGCAGCAGGCTTGCTGGCGCTATACCCCAGCGCAGGCACGCCCGCCGACCGCGCGGCAGTGATGCGCAGTg  >  5:12075/1‑135 (MQ=255)                                                                                                                                    |                                                                                                                                 GCTTGGCCATAACGAATGGATCCTGCAGCTTTTCCATGGGCCTACCCGGTCCTCCAAGGACTTTGCCGCGCAGTTGCAGGCGCGCTTTGTCCGGCACTTTCTGGGGGCAGAGTGCGAGCGGGTCATGCTGGTCGGTGCCAGCAACGGCGATACCGCAGTAGCGGCTATCGAAGCGTTGCGGCATTGCCCTGCAGCAGGCTTGCTGGCGCTATACCCCAGCGCAGGCACGCCCGCCGACCGCGCGGCAGTGATGCGCAGTG  >  ACB122/763645‑763904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑