| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 5,881,004 | G→A | 9.6% | M1I (ATG→ATA) † | quiP → | Acyl‑homoserine lactone acylase QuiP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 5,881,004 | 0 | G | A | 9.6% | 121.1 / 4.1 | 42 | M1I (ATG→ATA) † | quiP | Acyl‑homoserine lactone acylase QuiP |
| Reads supporting (aligned to +/- strand): ref base G (22/15); new base A (2/2); total (25/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01 | |||||||||||
TAGAGCTGGCCGGTGAGCATTTTGTGTTTTTCGCTGAGGGACATGGAAACTCCTTCCTGGGGCTATGCTCTGTTCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGGTACTGGTACGTTCAGGGCAAGCTGCCGCAGCGCGAGGGCGAGGTCGCCGTTGCCGGCCTGCAAGCGCCGGTCAGCGT > ACB122/5880872‑5881138 | tAGAGCTTGCCTGTGAGCATTTTGTGTTTTTCGCTGAGGTACATGGAAACTACTTCCTGGGGCTATGCTCTGTTCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCtgaatgaa < 6:54244/135‑1 (MQ=255)tAGAGCTGGCCGGTGAGCATTTTGTGTTTTTCGCTGAGGGACATGGAAACTCCTTCCTGGGGCTATGCTCTGTTCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCtgaatgaa < 6:54248/135‑1 (MQ=255) tGGCCGGTGAGCATTTTGTGTTTTTCGCTGAGGGACATGGAAACTCCTTCCTGGGGCTATGCTCTGTTCGCGAATCCGCCGATGATCGGACCCCATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAg > 7:290549/1‑135 (MQ=255) ccGGTGAGCATTTTGTGTTGTTCGCTGAGGGACATGGAAACTCCTTCCTGGGGCTATGCTCTGTTCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGcc < 5:267615/134‑1 (MQ=255) ggTGAGCATTTTGTGTTTTTCGCTGAGGGACATGGAAACTCCTTCCTGGGGCTATGCTCTGTTCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGTATGAAGCGCAGCCTGa > 4:118823/1‑135 (MQ=255) ggTGAGCATTTTGTGTTTTTCGCTGAGGGACATGGAAACTCCTTCCTGGGGCTATGCTCTGTTCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGa > 5:140442/1‑135 (MQ=255) ggTGAGCATTTAGTGTTTTTCGCTGAGGGACATGGAAACTCCTTCCTGGGGCTATGCTCTGTTCGCGAATCCGCCGATGATCGGACCACATTATTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGa > 7:219918/1‑135 (MQ=255) gCATTTTGTGTTTTTCGCTGAGGGACATGGAAACTCCTTCCTGGGGCTATGCTCTGTTCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCct < 1:273479/134‑1 (MQ=255) tggggttttCGCTGAGGGACATGGAAACTCCTTCCTGGGGCTATGCTCTGTTCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGc < 8:286883/129‑1 (MQ=255) tCGCTGAGGGACATGGAAACTCCTTCCTGGGGCTATGCTCTGTTCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATTAAGCGCAGCCTGACACTGCTGGCGGTGGTAg > 4:236264/1‑134 (MQ=255) aTGGAAACTCCTTCCTGGGGCTATGCTCTGTTCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGc < 1:293509/135‑1 (MQ=255) ggAAACTCCTTCCTGGGGCTATGCTCTGGTCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTg < 2:252447/135‑1 (MQ=255) cttcctGGGGCTATGCTCTGTTCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGc > 7:163307/1‑125 (MQ=255) cttcctGGGGCTATGCTCTGTTCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGc < 8:163307/125‑1 (MQ=255) cctGGGGCTATGCTCTGTTCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCgg > 5:86601/1‑134 (MQ=255) ggggTATGGTCTGTTCGAGATTCCGCGGATGATCGGACCTGATTTTTCGTTTGAATGCACTAGGAGTGTGTATGAAGCGCAGCCTGACCCTGCTTGCGGTGGTAGTCAGCGTGGCGGCTTGCGCCTCCGGGTACt < 8:219918/135‑1 (MQ=255) gggCTATGCTCTGTTCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGGTACt < 2:25251/135‑1 (MQ=255) ggCTATGCTCTGTTCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATTCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGGTACTg < 5:34481/135‑1 (MQ=255) ggCTATGCTCTGTTCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGGTACt < 1:161456/134‑1 (MQ=255) gCTATGCTCTGTTCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGGAGTCACCGTGGCCGCTGGCGCCTCCGGGTACTgg > 3:244856/1‑135 (MQ=255) tATGCTCTGTTCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGGTACTGGTa > 7:111500/1‑135 (MQ=255) aTGCTCTGTTCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGGTACTGGTAc > 2:251916/1‑135 (MQ=255) ctctGTTCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGGTACTGGTACGt > 4:203088/1‑134 (MQ=255) tctGTTCGCGAATCCGCCGATGATCGGACCACATATTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTCTCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGGTACTGCTACGt > 6:192361/1‑133 (MQ=255) gTTCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGGTACTGGTACGTTCa < 5:95772/133‑1 (MQ=255) gTTCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGGTACTGGTACGTTCa < 8:303573/133‑1 (MQ=255) tCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGATGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGGTACTGGTACGTTCAggg > 1:200424/1‑134 (MQ=255) tCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCggtg < 7:34264/87‑1 (MQ=255) tCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCggtg > 8:34264/1‑87 (MQ=255) tCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGGTACTGGTACGTTCAggg > 6:13463/1‑134 (MQ=255) tCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATCCTCAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGGTACTGGTACGTTCAgg < 8:6986/133‑1 (MQ=255) cgcgAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGGTACTGGGACGTTCAggg > 5:165349/1‑133 (MQ=255) cgcgAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGCTACTGGGACGTTCAggg > 5:168860/1‑133 (MQ=255) gcgAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCCGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGGTACTGGTACGTTCAGGGCaa > 5:221742/1‑135 (MQ=255) gcgAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGCCACCGTGGCCGCTGGCGCCTCCGGGTACTGGTACGTTCAGGGCaa > 2:239249/1‑135 (MQ=255) gcgAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATAAAGCGCAGCCTGACCctgc > 8:95651/1‑77 (MQ=255) gcgAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATAAAGCGCAGCCTGACCctgc > 8:100120/1‑77 (MQ=255) gcgAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATAAAGCGCAGCCTGACCctgc < 7:95651/77‑1 (MQ=255) gcgAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATAAAGCGCAGCCTGACCctgc < 7:100120/77‑1 (MQ=255) aaTCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGGTACTGGTACGTTCAGGGCAAGCt > 6:181532/1‑135 (MQ=255) cgATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGGTACTGGTACGTTCAGGGCAAGCTGCCGCAg > 7:232779/1‑135 (MQ=255) gACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGGTACTGGTACGTTCAGGGCAAGCTGCCGCAGCgcgagggc > 1:112437/1‑135 (MQ=255) aTGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGGTACTGGTACGTTCAGGGCAAGCTGCCGCAGCGCGAGGGCGAGGGCGCCGTTGCCGGcc < 1:268436/135‑1 (MQ=255) gCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGGTACTGGTACGTTCAGGGCAAGCTGCCGCAGCGCGAGGGCGAGGTCGCCGTTGCCGGCCt > 7:141942/1‑134 (MQ=255) tgactgaaGCGCAGCCGGACCCTGCTGGCGGTGGTAGGCACCGTGGCCGCTGGCGCCGCCGGGTACTGGTACGTTCAGGGCAAGCTGCCGCAGCGCGAGGGCGAGGTCGCCGTTGCCGGCCTGCAAGCGCCGGTc < 7:260978/135‑1 (MQ=255) atgaaGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGGTACTGGTACGTTCAGGGCAAGCTGCCGCAGCGCGAGGGCGAGGTCGCCGTTGCCGGCCTGCAAGCGCCGGTCAGc > 7:223595/1‑135 (MQ=255) gaaGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGGTACTGGTACGTTCAGGGCAAGCTGCCGCAGCGCGAGGGCGAGGTCGCCGTTGCCGGCCTGCAAGCGCCGGTCAGCGt > 7:126226/1‑135 (MQ=255) gaaGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGGTACTGGTACGTTCAGGGCAAGCTGCCGCAGCGCGAGGGCGAGGTCGCCGTTGCCGGCCTGCAAGCGCCGGTCAGCGt > 3:52530/1‑135 (MQ=255) | TAGAGCTGGCCGGTGAGCATTTTGTGTTTTTCGCTGAGGGACATGGAAACTCCTTCCTGGGGCTATGCTCTGTTCGCGAATCCGCCGATGATCGGACCACATTTTTCGTTTGAATGCACAAGGAGTCTGAATGAAGCGCAGCCTGACCCTGCTGGCGGTGGTAGTCACCGTGGCCGCTGGCGCCTCCGGGTACTGGTACGTTCAGGGCAAGCTGCCGCAGCGCGAGGGCGAGGTCGCCGTTGCCGGCCTGCAAGCGCCGGTCAGCGT > ACB122/5880872‑5881138 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |