Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 6,119,958 G→C 18.8% L195V (CTT→GTT)  gcvA_13 ← Glycine cleavage system transcriptionalactivator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1226,119,9580GC18.8% 69.6 / 2.5 44L195V (CTT→GTT) gcvA_13Glycine cleavage system transcriptionalactivator
Reads supporting (aligned to +/- strand):  ref base G (20/15);  new base C (4/5);  total (24/20)
Fisher's exact test for biased strand distribution p-value = 7.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAAGCCAGAGCCAGGTACACGACGACAACCCTTCGATGCTGCTCAGTTGCGGATGCCTGGCCAGATAGGCCGGGCTGCACACCAGGAAGATTTCTTCCGAGAACAGCGGCGTGACAGCCATGTCCTTGGGCGGTGGG  >  ACB122/6119828‑6120091
                                                                                                                                  |                                                                                                                                     
ccACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCGGGGGGGGTTCAagcccc                                                                                                                                   >  5:246002/1‑133 (MQ=255)
 cACTTATCGCCTACTGGATCTGCATACGTTAGCTGTTTTTTTTGGCGCGATGGTGGGGGGTCATGGGCTGGTATCCCAGGCGGTTGAACCATTCGGGCCAACCTACCCTGTCGCGGTGGGAGTCTTCAagccaga                                                                                                                                  <  2:130351/135‑1 (MQ=255)
         gCCTACTGGATAAGCATACCGTAGGTGTTTATTTTGACGCGAGGGTGGGGGGTCTGGGGCTCGTATCCCAGGCGCTTCAACAATTCGGGCCAACCAACCCAGTCGCGCTGGGAGTCTTCAAGCCAGAGCCTGGtc                                                                                                                          <  6:246002/135‑2 (MQ=255)
                 gATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAAGCCAGAGCCACGTACacgacc                                                                                                                   >  2:207559/1‑133 (MQ=255)
                         tACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGGCTTCGAGCCAGGCGCAGGTTAACGCCGAGCACCCtt                                                                                                          >  1:155916/1‑135 (MQ=255)
                            cGTAGGTGTTGCTTTTCACGCGATGCTGGGGGGTCCTGGGCTCGTAGCCCAGGCGCTTGAACCATTCGGCCCAACCTCCCCAGTCGCGCTGGTAGTCTTCAAGCCAGAGCCAGGTACACGACGACAACCCTTCg                                                                                                        <  4:71805/134‑1 (MQ=255)
                              tAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGCGAGTCTTCAAGCCAGAGCCAGGTACACGACGACCACCCTTCGCtg                                                                                                     >  7:41460/1‑135 (MQ=255)
                               aGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAACCCAGAGCCAGGTACACGACGACAACCCGTCGAtgc                                                                                                    >  6:217561/1‑135 (MQ=255)
                                 gtGTTGATATGGACGCGTGGCTTGGGGGTAATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCGGGGAGTCTTCAAGCCAGAGCCAGGTACACGACGACAACCCTTCGAtgct                                                                                                   <  7:273516/133‑1 (MQ=255)
                                   gTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCGGGGAGTCGTAAAGCCACAGCCAGGTAAACGACCACCACCCAACGAttcggcg                                                                                                >  2:124495/1‑129 (MQ=255)
                                    ttGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAAGCCAGCGCCAGGTACACGCCGCCAACCCTTCGAtgctgc                                                                                                 >  5:134123/1‑133 (MQ=255)
                                    ttGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAAGCCAGAGCCAGGTACACGACGACAACCCTTCGATGCTGCTc                                                                                               >  4:209349/1‑135 (MQ=255)
                                        tatTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAAGCCAGAGCCAGGTACACGACGCCAACCCTTCGCTGCTGCTCAGtt                                                                                           >  3:87185/1‑135 (MQ=255)
                                         atTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAAGCCAGAGCCAGGTACACGACGCCAACCCTTCGATGCTGCTCAGTTg                                                                                          >  8:9289/1‑135 (MQ=255)
                                              cgggATGCTTGGGGGTCATGGGCGCGTATCCCAGGCGAGTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAACCCAGAGCCAGGTACACGACGACAACCCTTCGATGCTGCTCAGTTGCGGa                                                                                      <  7:73796/131‑1 (MQ=255)
                                               gcgATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCGTGAAGCCAGAGCCAGGTACACGCCGACAACCCTTCGATGCTGCTCAGTTGCGGATg                                                                                    >  2:253758/1‑135 (MQ=255)
                                                cgcggCTTGGGGGGCCGGGGCCCGTACCCCAGGCGCTTGAACCATTCGGGCCAACCACCCCAGCCGCGCTGGGAGTCTACCCCCCAGAGCCAGGCCCACGACGACAACCCTTCGATGCTGCTCAGTTGCGGATGc                                                                                   <  5:20427/131‑1 (MQ=255)
                                                     cTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGTGTCTTCCAGCCAGCGCCCGGTACACGCCGCCAACCCTTCGATGCTGCTCAGTTGCGGATGCCGGGc                                                                              >  3:61259/1‑135 (MQ=255)
                                                      gtGGGGGGCTTGGGCTCTTACCCCCGGCGCTTCAACCTTTCGGGCCAACCACCCCAGGCGGGCGGGGAGGCTTCCCGCCAGCGCCAGGTACACGACGACAACCCTTCGATGCTGCTCAGTTGCGGATGCCTGGcc                                                                             <  5:217561/134‑1 (MQ=255)
                                                          gggTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAAGCCAGAGCCAGGTACACGACGACAACCCTTCGATGCTGCTCAGTTGCGGATGCCTGGCCAg                                                                           >  4:128395/1‑133 (MQ=255)
                                                               aTGGGCTCGTATCCCAGGCGCTTGAACCCTTCGGGCCAACCTCCCCAGCCGCGCGGGGAGGCTTCAAGCCAGAGCCAGGTACACGACGACAACCCTTCGATGCTGCTCAGTTGCGGATGCCTGGCCAGATAGGcc                                                                    <  3:269058/135‑1 (MQ=255)
                                                                tGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGGTGGGGGTCTTCAACCCCCCGCCAGGTACCCCACGACAACCCTTCGAGTCTGCTCAGTTGCGGATGGCTGGCCAGCTATGCCg                                                                   >  5:252028/1‑135 (MQ=255)
                                                                tGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAAGCCAGAGCCAGGTACACGACGACAACCCTTCGATGCTGCTCAGTTGCGGATGCCTGGCCAGATAGGCCg                                                                   >  7:164813/1‑135 (MQ=255)
                                                                  ggCGCGTAGCCCCGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAAGCCAGAGCCAGGTACACGACGACAACCCTTCGATGCTGCTCAGTTGCGGATGCCTGGCCAGATAGGCCggg                                                                 <  5:206336/135‑1 (MQ=255)
                                                                   gcccgaaTCCCAGGCGCTTGACCCATGCGGGCCACCCTACCCCGCCGCGCTGGGAGTCTTCAACCCAGACCCCGGAACACGACGACAACCCTTCGATGCTGCTCAGTTGCGGATGCCTGGCCAGATAGGCCGGGc                                                                <  3:65819/129‑1 (MQ=255)
                                                                    cTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAAGCCAGAGCCAGGTACACGACGACAACCCTTCGATGCTGCTCAGTTGCGGATGCCTGGCCAGATAGGCCGGGCt                                                               <  3:209349/135‑1 (MQ=255)
                                                                       gTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGGGGGGTGTGTTCAAGCCCGCGCCAGGTGCACGCCGCCCCCCCGTCGCTGGTGGGCAGGTGCGGCTGCCGGGCCCGATAGGCCGggcggcg                                                            >  5:257105/1‑134 (MQ=255)
                                                                                gcccTTGACCCATTCGGCCCAACCTACCCCGGCGCGCTGGGAGTCTTCAACCCACAGCCAGGACCACGACGACAACCCTTCGATGCTGCTCAGTTGCGGATGCCTGGCCAGATAGGCCGGGCTGCACACCAGGaa                                                   <  3:200014/132‑1 (MQ=255)
                                                                                  gcTTGAACCATTCGGGCCAACCAACCCAGGCGCGCTGGGAGTCTTCACGCCAGAGCCAGGTCCACGACGACAACCCTTCGATGCTGCTCAGTTGCGGATGCCTGGCCAGATAGGCCGGGCTGCACACCAGGAAGa                                                 <  7:301642/135‑1 (MQ=255)
                                                                                      gAACCATTCGGGCCAACCTACCCAGTCGCGCGGGGAGTCTTAAACCCACTGGCCGGGACACCACGACAACCCATCGATGGTGCTAAGTTTAGGATTCCATGGCACTTATGCCCGGCAGCACAACAAGAAGAttta                                             >  2:79535/1‑134 (MQ=255)
                                                                                               tggCCAACCTACCCAGTCGCGCTGGGAGTATTAAACCAAGATCCAGGTACACGAACAAAACACTTCGATTCTGATAAGTTGAGGAAGACACGCCATATAGGCACGGCTGAAAAACATGAAGAATTATAACGAGaa                                    >  8:259006/2‑135 (MQ=255)
                                                                                               gggCCACCCTACCCAGTCGCGCTGGGAGTCTTCAAGCAAGAGCCAGGTAAACGCCGACAACACTTCGATGCTGCTCAGTTGCGGAAGCCTGGACAGATAGGCAGGGCTGAACACCAGGAAGAATTCTTCCgaga                                     >  8:183078/1‑134 (MQ=255)
                                                                                                    aaCCTACCCAGTCGCGCTGGGAGTCTTCAAGCCCGAGCCAGGTACACGACGACAACCCGTCGATGCTGCGCAGTTGCGGATGCCTGGCCAGATAGGCCGGGCTGCACACCACGAAGATTTATTCCGAGAACAgcg                               >  1:82568/1‑135 (MQ=255)
                                                                                                            ccggcgcgGTGGGAGTGTTCACGCCAGAGCCAGGTCCACGACGACAACCCTTCGATGCTGCTCAGTTGCGGATGCCTGGCCAGATAGGCCGGGCTGCACACCAGGAAGATTTCTTCCGAGAACAGCGGCGTGACa                       <  7:142736/131‑1 (MQ=255)
                                                                                                             cggcgcgCTGGGAGTCTTCAAGCCATAGCCAGGAACACGACGACAACCCTTCGATGCTGCTCAGTTGCGGATGCCTGGCCAGATAGGCCGGGCTGCACACCAGGAAGATTTCTTCCGAGAACAGCGGCGTGACAg                      <  8:166731/132‑1 (MQ=255)
                                                                                                             cgTCGCGGTGGGAGTCTTCACGCCAGAGCCAGGAACACGACGACAACCCTTCGATGCTGCTCAGTTGCGGATGCCTGGCCAGATAGGCCGGGCTGCACACCAGGAAGATTTCTTCCGAGAACAGCGGCGTGACAg                      <  1:207559/134‑1 (MQ=255)
                                                                                                                  cgcgGGGAGTCTTCAAGCCAGAGCCAGGTACACCACGCCAACCCGTCGATGGTGCTCAGTTTCGGATGCCTGGCCAGATAGGCCCGGCTGCACACCAGGAAGATTTCTCCCGAGAACAGCGGCGTGGCCGCCCTg                 >  7:138297/1‑135 (MQ=255)
                                                                                                                  cgcTGGGAGTCTTCAAGCCAGAGCCAGGTACACGACGACAACCCTTCGATGCTGCTCAGTTGCGGATGCCTGGCCAGATAGGCCGGGCTGCACACCCGGAAGATTTCTACCGAGAACAGCGGCGTGCCAGCCATg                 >  5:294837/1‑135 (MQ=255)
                                                                                                                  cgcTGGGAGTCTTCAAGCCAGAGCCAGGTACACGACGACAACCCTTCGATGCTGCTCAGTTGCGGATGCCTGGCCAGATAGGCCGGGCTGCACACCAGGAAGATTTCTTCCGAGAACAGCGGCGTGACAGCCATg                 >  5:155267/1‑135 (MQ=255)
                                                                                                                   gcTGGGATCCTTCAAGCCAGCGCCAGGTACACGACGACAACCCTTCGATGCTGCTCAGTTGCGGATGCCTGGCCAGATAGGCCGGGCTGCACACCAGGAAGATTTCTTCCGAGAACAGCGGCGTGACAGCCATGt                <  3:40135/135‑1 (MQ=255)
                                                                                                                   gcTGGGAGTCTTCAACCCAGAGCCAGGTACACGACGACAACCCTTCGATGCTGCTCAGTTGCGGATGCCTGGCCAGATAGGCCGGGCTGCACACCAGGAAGATTTCTTCCGAGAACAGCGGCGTGACAGCCATGt                <  7:189406/135‑1 (MQ=255)
                                                                                                                         aGTCTTCAAGCCAGAGCCAGGTACACGACGACAACCCTTCGATGCTGCTCAGGTGCGGATGCCTGGCCAGATAGGCCGGGCTGCACACCAGGAAGATTTCTTCCGAGAACAGCGGCGTGACAGCCATGTCCTTgg          <  4:61259/135‑1 (MQ=255)
                                                                                                                                aaGCCAGAGCCAGGTCCACGACGACAACCCTTCGATGCTGCTCAGTTGCGGATGCCTGGCCAGATAGGCCGGGCTGCACACCAGGAAGATTTCTTCCGAGAACAGCGGCGTGACAGCCATGTCCTTGGGCGGTg    <  6:246007/134‑1 (MQ=255)
                                                                                                                                aaGCCAGAGCCAGGTACACGACGACAACCCTTCGATGCTGCTCAGTTGCGGATGCCTGGCCAGATAGGCCGGGCTGCACACCAGGAAGATTTCTTCCGAGAACAGCGGCGTGACAGCCATGTCCTTGGGCGGTgg   >  2:31559/1‑135 (MQ=255)
                                                                                                                                aaGCCAGAGCCAGGTACACGACGACAACCCTTCGATGCTGCTCAGTTGCGGATGCCTGGCCAGATAGGCCGGGCTGCACACCAGGAAGATTTCTTCCGAGAACAGCGGCGTGACAGCCATGTCCTTGGGCGGTgg   >  8:175005/1‑135 (MQ=255)
                                                                                                                                aaGCCAGAGCCAGGTACACGACGACAACCCTTCGATGCTGCTCAGTTGCGGATGCCTGGCCAGATAGGCCGGGCTGCACACCAGGAAGATTTCTTCCGAGAACAGCGGCGTGACAGCCATGTCCTTGGGCGGTg    <  6:246009/134‑1 (MQ=255)
                                                                                                                                 agccagagccagGTACACGACGACAACCCTTCGATGCTGCTCAGTTGCGGATGCCTGGCCAGATAGGCCGGGCTGCACACCAGGAAGATTTCTTCCGAGAACAGCGGCGTGACAGCCATGTCCTTGGGCGGTggg  <  6:134123/135‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                     
CCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAAGCCAGAGCCAGGTACACGACGACAACCCTTCGATGCTGCTCAGTTGCGGATGCCTGGCCAGATAGGCCGGGCTGCACACCAGGAAGATTTCTTCCGAGAACAGCGGCGTGACAGCCATGTCCTTGGGCGGTGGG  >  ACB122/6119828‑6120091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑