Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 6,182,018 C→T 7.7% S222N (AGC→AAC)  yidC ← Membrane protein insertase YidC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1226,182,0180CT 7.7% 165.7 / 2.6 52S222N (AGC→AAC) yidCMembrane protein insertase YidC
Reads supporting (aligned to +/- strand):  ref base C (22/26);  new base T (2/2);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACACATTTTCTTTCAAACTACCTTTGTCGATGTCCTTCATCGAGACCTTTTTGTAAGGCTCAGAAGCTGTCCACAGGGCAGCACCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACATTCAGGTCGTACTCACCGCGCTTGAAGCTGAAACGCTTGATGTAGTTGACAC  >  ACB122/6181887‑6182147
                                                                                                                                   |                                                                                                                                 
acacATTTTCTTTCAAACTACCTTTGTCGATGTCCTTCATCGAGACCTTTTTGTAAGGCTCAGAAGCTGTCCACAGGGCAGCACCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTgg                                                                                                                                <  6:145458/135‑1 (MQ=255)
acacATTTTCTTTCAAACTACCTTTGTCGATGTCCTTCATCGAGACCTTTTTGTAAGGCTCAGAAGCTGTCCACAGGGCAGCACCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTgg                                                                                                                                <  4:275336/135‑1 (MQ=255)
acacATTTTCTTTCAAACTACCTTTGTCGATGTCCTTCATCGAGACCTTTTTGTAAGGCTCAGAAGCTGTCCACAGGGCAGCACCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTgg                                                                                                                                <  4:126965/135‑1 (MQ=255)
acacATTTTCTTTCAAACTACCTTTGTCGATGTCCTTCATCGAGACCTTTTTGTAAGGCTCAGAAGCTGTCCACAGGGCAGCACCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTgg                                                                                                                                <  3:32261/135‑1 (MQ=255)
acacATTTTCTTTCAAACTACCTTTGTCGATGTCCTTCATCGAGACCTTTTTGTAAGGCTCAGAAGCTGTCCACAGGGCAGCACCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTgg                                                                                                                                <  2:194924/135‑1 (MQ=255)
acacATTTTCTATCAAACTACCTTTGTCGATGTCCTTCATCGAGACCTTTTTGTAAGGCTCAGAAGCTGTCCACAGGGCAGCACCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTgg                                                                                                                                <  2:267392/135‑1 (MQ=255)
             cAAACTACCTTTGTCGATGTCCTTCATCGAGACCTTTTTGTAAGGCTCAGAAGCTGTCCACAGGGCAGCACCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCtt                                                                                                                      <  5:299198/132‑1 (MQ=255)
             cAAACTACCTTTGTCGATGTCCTTCATCGAGACCTTTTTGTAAGGCTCAGAAGCTGTCCACAGGGCAGCACCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCtt                                                                                                                      <  2:111105/132‑1 (MQ=255)
                           cGATGTCCTTCATCGAGACCTTTTTGTAAGGCTCAGAAGCTGTCCACAGGGCAGCACCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATg                                                                                                      <  5:66697/134‑1 (MQ=255)
                           cGATGTCCTTCATCGAGACCTTTTTGTAAGGCTCAGAAGCTGTCCACAGGGCAGCACCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGt                                                                                                     >  8:152774/1‑135 (MQ=255)
                                             ccTTTTTGTAAGGCTCAGAAGCTGTCCACAGGGCAGCACCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGATGCCGTTCCAGGCCTGGc                                                                                   >  6:204107/1‑135 (MQ=255)
                                                          cTCAGAAGCTGTCCACAGGGCAGCACCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGt                                                                       >  6:73998/1‑134 (MQ=255)
                                                           tCAGAAGCTGTCCACAGGGCAGCACCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCg                                                                     >  2:124068/1‑135 (MQ=255)
                                                           tCAGAAGCTGTCCACAGGGCAGCACCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCg                                                                     >  2:226586/1‑135 (MQ=255)
                                                               aaGCTGTCCACAGGGCAGCACCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCa                                                                 <  2:228498/135‑1 (MQ=255)
                                                                      ccACAGGGCAGCACCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGc                                                           <  8:94424/134‑1 (MQ=255)
                                                                       cacaGGGCAGCACCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTg                                                         >  1:262536/1‑135 (MQ=255)
                                                                       cacaGGGCAGCACCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTg                                                         >  4:12414/1‑135 (MQ=255)
                                                                          aGGGCAGCACGCAGGTAGTTCGCGGTGCCGGTGGCAGTACTGGAGGACGTATCGCCGCTGGCATCACGCTTGAGCTTGGAAAACATGTTGCCGTTCCAGGCCTTGCCGCGCTGGTTGTCGTTCAGGTAGCTGACa                                                      <  7:212080/135‑1 (MQ=255)
                                                                          aGGGCAGCACCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACa                                                      <  1:189126/135‑1 (MQ=255)
                                                                          aGGGCAGCACCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACa                                                      <  2:258573/135‑1 (MQ=255)
                                                                          aGGGCAGCACCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACa                                                      <  8:43406/135‑1 (MQ=255)
                                                                          aGGGCAGCACCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGAGCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACa                                                      <  3:237507/135‑1 (MQ=255)
                                                                          aGGGCAGCACCCAGGGAGGGCGCGGTGCCGGTGGCAGTACTGGAGGACGGAGCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACa                                                      <  2:84816/135‑1 (MQ=255)
                                                                               agcaCGCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACa                                                      <  2:131362/130‑1 (MQ=255)
                                                                               agcaCCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACa                                                      >  1:131362/1‑130 (MQ=255)
                                                                                  aCCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACATTCAGGTc                                              >  1:280751/1‑135 (MQ=255)
                                                                                  aCCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACATTCAGGTc                                              >  5:168293/1‑135 (MQ=255)
                                                                                    ccAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACATTCAGGTCGt                                            >  8:163928/1‑135 (MQ=255)
                                                                                       ggtaggtCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACATTCAGGTCGTACt                                         <  6:168293/135‑1 (MQ=255)
                                                                                         taggtCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCCTCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGGTGTCGATCAGGTAGCTGACATTCAGGTCGTACTCa                                       >  3:279386/1‑135 (MQ=255)
                                                                                             tCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACATTCAGGTCGTACTCACcgc                                   <  8:291669/135‑1 (MQ=255)
                                                                                              cgcgGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGCCATTCAGGTCGTACTCACcgcg                                  >  5:81244/1‑135 (MQ=255)
                                                                                              cgcgGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACATTCAGGTCGTACTCACcgcg                                  >  4:229268/1‑135 (MQ=255)
                                                                                              cgcgGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACATTCAGGTCGTACTCACcgcg                                  >  3:240698/1‑135 (MQ=255)
                                                                                                 ggTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACATTCa                                                  <  3:213171/116‑1 (MQ=255)
                                                                                                 ggTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACATTCa                                                  >  4:213171/1‑116 (MQ=255)
                                                                                                 ggTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACATTCAGGTCGTACTCACCGCGCt                                >  7:163016/1‑134 (MQ=255)
                                                                                                   tGCCGGTGGCAGTACTGGAGGACGGATCGCCGTTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGCCCTTCAGGTCGGACTCACCGCGCGTg                              >  2:293416/1‑134 (MQ=255)
                                                                                                   tGCCGGTGGCAGTACTGGAGGACGGATCGCCGTTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCGCTGGGTGTCGATCAGGTAGCTGGCATTCCGGTCGGACTCCCCccgcgg                               >  2:293415/1‑131 (MQ=255)
                                                                                                   tGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTgg                                                                                                                                >  7:287598/1‑36 (MQ=255)
                                                                                                   tGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTgg                                                                                                                                <  8:287598/36‑1 (MQ=255)
                                                                                                   tGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGCCAGTCAGGTCGTACTCCCCGCGCtt                               >  3:253674/1‑133 (MQ=255)
                                                                                                            cAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACATTCAGGTCGTACTCACCGCGCTTGAAGCTGAAAc                    <  7:163928/135‑1 (MQ=255)
                                                                                                                  tGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACATTCAGGTCGTACTCACCGCGCTTGAAGCTGAAACGCTTGa              <  8:163016/135‑1 (MQ=255)
                                                                                                                       gaCGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACATTCAGGTCGTACTCCACGCGCGTGAAGCTGAAAAGCTTGATGTAg         >  1:53102/1‑135 (MQ=255)
                                                                                                                          ggATCGCCGTTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACATTCAGGTCGTACTCACCGCGCTTGAAGCTGAAACGCTTGATGTAGTTg      <  1:293415/135‑1 (MQ=255)
                                                                                                                          ggATCGCCGTTGGCATCACGCTGGAGCTGGGCAAACATGTTGCCGTGCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACATTCAGGTCGTACTCACCGCGCTTGAAGCTGAAACGCTTGATGTAGTTg      <  1:293416/135‑1 (MQ=255)
                                                                                                                           gATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACATTCAGGTCGTACTCACCGCGCTTGAAGCTGAAACGCTTGATGTAGTTGa     <  6:68481/135‑1 (MQ=255)
                                                                                                                            aTCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTa                                                             <  1:163112/78‑1 (MQ=255)
                                                                                                                            aTCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTa                                                             >  2:163112/1‑78 (MQ=255)
                                                                                                                            aTCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACATTCAGGTCGTACTCACCGCGCTTGAAGCTGAAACGCTTGATGTAGTTGac    >  7:205118/1‑135 (MQ=255)
                                                                                                                              cgccgcTGGCATCACGCTTGAGCTGTGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACATTCAGGTCGTACTCACCGCGCTTGAAGCTGAAACGCTTGATGTAGTTGacac  <  2:106429/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                 
ACACATTTTCTTTCAAACTACCTTTGTCGATGTCCTTCATCGAGACCTTTTTGTAAGGCTCAGAAGCTGTCCACAGGGCAGCACCCAGGTAGGTCGCGGTGCCGGTGGCAGTACTGGAGGACGGATCGCCGCTGGCATCACGCTTGAGCTGGGCAAACATGTTGCCGTTCCAGGCCTGGCCGCTCTGGTTGTCGATCAGGTAGCTGACATTCAGGTCGTACTCACCGCGCTTGAAGCTGAAACGCTTGATGTAGTTGACAC  >  ACB122/6181887‑6182147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑