| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 868,330 | T→G | 43.9% | intergenic (+19/+27) | loiP → / ← mneA | Metalloprotease LoiP/Putative manganese exporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 868,330 | 0 | T | G | 43.9% | ‑2.5 / 3.9 | 20 | intergenic (+19/+27) | loiP/mneA | Metalloprotease LoiP/Putative manganese exporter |
| Reads supporting (aligned to +/- strand): ref base T (8/2); new base G (7/2); total (16/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01 | |||||||||||
CACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCC‑GGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTC‑ATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCA > ACB122/868202‑868445 | cACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATgg > 8:296149/1‑135 (MQ=255) aCCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCtggctggc > 2:196614/1‑135 (MQ=255) aCCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGACAAGCCGTAAATGCCTGAATCCTGGGGCTGCagtgc > 7:176780/1‑131 (MQ=255) ttCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCCAGGCAGcccccccc > 4:130743/1‑134 (MQ=255) ttCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGAGTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAACGGCCTGAATCCTGGGGCGGCCTGGCCGcccccccc > 3:79613/1‑134 (MQ=255) tCATGAGTACTCCCCCGGCGGCCGCGAGCCGCATTGCTGCGCTGCAGGCGGCGATTCCGACGGTGATGCCGCGGTATCCGGCGGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCC‑gg < 7:294916/135‑1 (MQ=255) gAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCGGCCTGGGCGccccccccgggtt > 3:297403/1‑129 (MQ=255) gTACTCACCCGGCGCCCGCGAGCCGCATGGCTTCGCGGCCGGGGGCTATTCCCAAGGTTAGGCCGCTGTATCAGGCGGCCGCCAAGCCGTAAATGCCTTAATCGTGGGGCTGCATGGCAGCCCCCCC‑GGTTTTc < 6:119185/134‑1 (MQ=255) aCCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCCTGGGAGCCCCCCC‑GGTGTTGCCTTTAc > 8:209475/1‑135 (MQ=255) aCCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCGGGCTGGCCGCCCCCCCGGGTTTTCCGtttg > 6:281871/1‑134 (MQ=255) ccgggcggcCGCGTGCCGAATTGCTGCGCTGCTGGCGGGGATTCCGAAGGTGATGCCGCTGTATCTGGCTGCCGCCAAGCGTTAAATGCCTGAATCCTGGGGCGGAATGGCAGCCCCCCC‑GGTTTTCCCTTTa < 7:296149/130‑1 (MQ=255) tCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCGGCATGGGCCCCCCCCC‑GGTGTTACACTTACCCaaaacc > 3:148833/1‑131 (MQ=255) gAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCGGGCTGGGAGCCCCCCCGGGTTTGCCGTTTGCCCAAGccgcc > 1:219801/1‑130 (MQ=255) tGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCC‑GGGTTTGGCGTTACCCCAACCCGCCCCTGGCCGTG‑ATTGccca > 7:93556/1‑132 (MQ=255) cAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCGGCCTTGGAGCCCCACC‑GGGTTTAGCATTTCCCCATCCCGACACAGGGCGAG‑ATTGCCGaaa > 7:209853/1‑133 (MQ=255) tCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCCTGGCCGCCCCCCC‑GGTTTTCCCTTTTCCCCATCCACCCCCTGGCCGTA‑ATTGGCGAATAGACGGcccccc > 2:222676/1‑131 (MQ=255) gCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCGGGATGGCCGCCCCCCC‑GGGTTTTCCGTTACCCCATCCAGCCCCTGGCCGTC‑ATTGCCGCATAGACGGCCACCCTTGCCAggaaga > 7:85928/1‑130 (MQ=255) ccGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGGATGGCCGCCCCCCC‑GGGTTTGCCGTTTACCAATACCCCCACGGGGCGTCAATTGCC‑AATAGACGGCCACGCTTGACAGttaga > 8:225339/1‑130 (MQ=255) aagccggcggcCGCCACGCCTAAAATCCCTTAAACCTGGGGGGGCATGGCCGCCCCCCC‑GGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTC‑ATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGc < 3:48867/130‑1 (MQ=255) cGTAAATGCCTGAATCCTGGGGGAGAAAGGCACCCCCCCC‑GGTTTTCGCGTTTCCCAATCCATCCACTGGCCTTG‑AATTCGGGAGAATCCGCCCCGAATTCCACTACGAAGCACGCCGATGCAGCCCggggggg > 8:226921/1‑132 (MQ=255) aaTGCCTGAATCCTGGGGCTGCCTGGCAGCCCCCCC‑GGGTTTTCCGTTTCCCAATCCAGCCACTTGCCGTC‑AATTGCGAATATACGGCCCCCATTGCCCGGACGAAGAACCCCCTTGCCCCCCCGCGGCGCATCa > 8:4417/1‑135 (MQ=255) | CACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCCC‑GGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTC‑ATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCA > ACB122/868202‑868445 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |