| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 2,834,922 | 0 | G | T | 46.2% | 1.1 / 17.5 | 26 | intergenic (‑44/+208) | yqhD/CJ019_02688 | Alcohol dehydrogenase YqhD/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (6/8); new base T (12/0); total (18/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.24e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGCTTCAGTCGGAATCAGCTCATCCAGCCGGGCAATGGTTTGGTTACCGAAGATAATTCGCGTGGGGTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGA > ACB122/2834788‑2835046 | gggCTTCAGTCGGAATCAGCTCATCCAGCCGGGCAATGGTTTGGTTACCGAAGATAATTCGCGTGGGGTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTg > 2:9298/1‑135 (MQ=255) gTCGGAATCAGCTCATCCAGCCGGGCAATGGTTTGGTTACCGAAGATAATTCGCGTGGGGTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTGGATTTTTTTGTGaaaat > 5:176454/1‑133 (MQ=255) cGGAATCAGCTCATCCAGCCGGGCAATGGTTTGGTTACCGAAGATAATTCGCGTGGGGTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTGTATTTTTTGGTGAAAtttt > 5:55105/1‑135 (MQ=255) aTCAGCTCATCCAGCCGGGCAATGGTTTGGTTACCGAAGATAATTCGCGTGGGGTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTGATTTTTTTTGTGGAATTTTTAtt > 4:197238/1‑135 (MQ=255) gggCAATGGTTTGGTTACCGAAGATAATTCGCGTGGGGTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTTTATTTTTTTTTGTAATTTTTTTTTCAGAAGCTCGTAggg > 1:164509/1‑134 (MQ=255) gggCAATGGTTTGGTTACCGAAGATAATTCGCGTGGGGTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGgc > 8:161885/1‑135 (MQ=255) aTGGTTTGGTTACCGAAGATAATTCGCGTGGGGTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTTTATTTTTTTGTGGAATTTTTTTTTCTTGAGCTCGTGGgcgcgcc > 2:133775/1‑134 (MQ=255) aTGGTTTGGTTACCGAAGATAATTCGCGTGGGGTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTGTTTTTTTTTGTGTAATTTTTATTTCAGAAGCTAGTCGgcgcgcc > 3:60745/1‑134 (MQ=255) gtttggttACCGAAGATAATTCGCGTGGGGTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCAtt < 2:54102/134‑1 (MQ=255) gttACCGAAGATAATTCGCGTGGGGTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCa < 7:71750/134‑1 (MQ=255) aaTTCGCGTGGGGTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGTGCACCAAATAGCTGaa < 7:161885/135‑1 (MQ=255) aaTTCGCGTGGGGTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGa < 7:23590/134‑1 (MQ=255) cgcgTGGGGTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTGTATTTTTTGTTTTAATTTTTTTTGGATAAGATCGTCGGCGCGCGGTGGGCACCCAATAGCTgtatctt > 5:3461/1‑129 (MQ=255) gcgTGGGGTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTTTATTTTTTTGTGTAATTTTTATTTGAGAAGCTCGTCGGCGCGCATTGCGCACCAAAAAGCAGAAGATTg > 5:63546/1‑135 (MQ=255) ggggTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTTGTGTAATTTTTTTTTCAGAAGCTCGTCGGCGCGCCTTTCGCACCCAATAGCTGAAGATTTGtt > 1:38392/1‑134 (MQ=255) ggtgtgGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGaa < 2:30280/133‑1 (MQ=255) aaGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTTTATTTTTTTGTGTAAATTTTTTTTCAGAAGCTAGTCGGCGCCCATTGAGCACCAAAAAGCAGAATATTTGTTATAAgcttgc > 7:118881/1‑134 (MQ=255) tCAAAATTGAGCATAGATTTCTCTCCTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCt < 3:7768/135‑1 (MQ=255) ttGAGCATAGATTTCTATACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGc < 1:183795/135‑1 (MQ=255) aTAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTTGTGTAATTTTTTTTTGAGAAGCTCGTCGGCGCGCATTTCGCCCCAAAAAGCTGAATATTTGTTTTAATCTTTGTTATAAAAGCaaa > 5:110853/1‑131 (MQ=255) aTAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTTCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCATGCTTCTAAAGGAAACCaa > 4:151319/1‑135 (MQ=255) aTTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTg < 2:146367/135‑1 (MQ=255) ttAAAAGTAGACCAGTCGTATATTTTTTTGTGAAATTTTTATTTGAGAAGCGAGTCGGCGCGCATTGCGCCCCAAATAGCTGAAGATTGGTTTGATGCTTGCTTGTAAAGGCAACCAAGTTGGTGTGGCATTCGt > 2:112199/1‑135 (MQ=255) ttAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTTTTGGAGAAGCTCGTAGGCGCGCATTGCGCACCAAATATCTGAAGATTGGTTTGTAGCTTGCTTGTAAAGGCAACCAACTTGGTGTGGCCTTCGt > 4:41218/1‑135 (MQ=255) cAGTCGTATATTTTTTTGTGTAATTTTTATTGCAGAAGCTCGTCGGCGCGCCTTGCGCCCCAAAAACCTGAAGATTGGTTAGAAGCTTTCGTCTAAGGGCAACCAACGTGGGTTGGCATTCGTCTGCAATCCTgg > 6:97626/1‑135 (MQ=255) gtttatTTTTTGGTGAAATTTTTATTGGAGAAGCTCGTCGGCGCCCATTGCGCACCAAATAGCTGAAGATTTGTTTTTAGCTTGCTTCTAAAGGCCACCCAGTTGGGGTGGGCTTCGGCTGGATTCCGGGTACCg > 4:204168/4‑135 (MQ=255) ttttttttttTGTGTAATTTTTTTTTGAGAAGCTCGTCGGCGCGCCTTTCGCCCCAAATAGATGTAGATTTGTTTGAATCTTTGTTATAAATGCAACCAAGTTGGTGTGGGATTGGTCTTGCTTCCGGGTCCCgc > 2:133860/5‑134 (MQ=255) | GGGCTTCAGTCGGAATCAGCTCATCCAGCCGGGCAATGGTTTGGTTACCGAAGATAATTCGCGTGGGGTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGA > ACB122/2834788‑2835046 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |