| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,210,881 | 0 | T | G | 52.9% | 11.6 / 19.0 | 17 | V204G (GTC→GGC) | oprJ_2 | Outer membrane protein OprJ |
| Reads supporting (aligned to +/- strand): ref base T (7/1); new base G (0/9); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.53e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGTGCCGCACGCATCGCCCTGATCGCCGAGGTGAGCCAGGCCTACCTGGCCTTGGACGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGACCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGCGCCGCCGG‑CACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAGCGCCAGGCCTACAACGCGCTGG > ACB122/3210756‑3211009 | gCGGGCCGCACGCACCGCCGGGACCGCCCTGGGTACCCAGGCCTACCTGGCCTTGGCCGGCGCCGAGCGGCGCCAGGCGCTGACCCGGCAGACCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAgcg < 8:155722/135‑1 (MQ=255) cGAGGGGCGCCCGGCCTCCCTGGCCGTGGCCGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGACCCGGGCCAGCCGCTCGGAGGCCCTGGCGGGGGGCGGCCAGCGGCGCGCCGCCGG‑CACGGCCACGGCGCt < 8:121379/135‑1 (MQ=255) gAGGTGAGCCAGGCCTACCTGGCCTTGGACGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGACCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGCGCCGCCCG‑cccgcccccggcccgg > 6:167400/1‑131 (MQ=255) tGGCCTTGGCGGGCCCCGCGGGGCGCCTGGGGCTGACCCGTCCCACCCGGGCCAGCCGCGAGGATGCCCTGGCGTGGGGCGGCCAGCGGGGCGCCGCCGG‑CACGGCCACGGCGCTGGACCACCAAGAGGCGCTgg < 7:132978/135‑1 (MQ=255) tGGACGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGACCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGCGCCGCCCG‑CCCGGCCCCGGCGCAGGCCCCCCCAGAGGCGCGgggggggg > 3:59638/1‑131 (MQ=255) tGGACGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGACCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGCGCCCCCCG‑CCCCGCCCCCGCGCCGGGCCCCCCAGACGCGCGGGGGCggg > 3:58058/1‑135 (MQ=255) gccGGCGCCGAGCGGCGCCAGGCGCTGCCCCGTCACACCCGGGCCAGCCGCGAGGATTCCCTGGCGTTGGGCGGCCAGCGGCGCGCCGCCGG‑CACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTc < 6:83880/133‑1 (MQ=255) aCGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGACCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGCGCCGCCGG‑CCCGGCCCCGGCGCTGGCCCCCCAAGAGGCGCTGGGGCTGGt > 2:17764/1‑133 (MQ=255) cGGCGCCGCGCGGCGCCCGGCGCGCCCCCGTCCGACCCGGGCCAGCCGCGAGGATGCCGGGGCGGTGGGCGCCCCGGGGCGCGCCGCCGG‑CACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGa < 6:113312/135‑1 (MQ=255) cAGGCGCTGACCCGTCAGACCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGCGCCGCCGG‑CCCGCCCCCGGCGCTGGGCCCCCAAGAGGCGCTGGGGCGGGTAGCGCAGggccgggccgcg > 7:166424/1‑133 (MQ=255) gACCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGCGCCGCCGG‑CACGGCCCCCGCGCTGGCCCCCCCACAGGCGCGGGGGGTGGGGGGGCAGTCCCCGGGCGCGCGCGCAGTGACCGGgcg > 1:122731/1‑135 (MQ=255) ccccTGGCCAGCCGCGAGGATGCCCTGGCGTGGGGCGCCCCGCGGCGCGCCGCCGG‑CACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTgcgc < 8:16358/134‑1 (MQ=255) ccccGGGCCAGCCGCGAGGATGCCCTGGCGTGGGGCGGCCCGCGGCGCGCCGCCGG‑CACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTgcgc < 1:17764/134‑1 (MQ=255) gCCAGCCGGGCGGATTCGCTGGCGTGGGGCGGCCCGGGGCGCGCGGCCGG‑CAGGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAg < 3:147192/135‑1 (MQ=255) ccAGCCGCGAGGATTCCCTGGGGTTGGGCGGCCAGCGGCGCGCCGCCGG‑CACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAg < 3:97266/134‑1 (MQ=255) cTGGCGTTGGTCGGCCAGCGGCGCGCCGCCCG‑CACGGCCACGGCCCTGGGCCCCCAAGAGGCGCGGGGGCTGGTCGACCAGTCCCCGGCCGAGCGAGAAGTCACCCTGCCCCAACAGCGCCACGCCTACAAcgcg > 6:89762/1‑135 (MQ=255) ggtGGGCGGCACGCGGGGCGCCGGCGGCCCCGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAGCGCCAGGCCTACAACGCGCTgg < 1:175683/133‑1 (MQ=255) | GCGTGCCGCACGCATCGCCCTGATCGCCGAGGTGAGCCAGGCCTACCTGGCCTTGGACGGCGCCGAGCGGCGCCAGGCGCTGACCCGTCAGACCCTGGCCAGCCGCGAGGATTCCCTGGCGTTGGTCGGCCAGCGGCGCGCCGCCGG‑CACGGCCACGGCGCTGGACCACCAAGAGGCGCTGGGGCTGGTCGAGCAGTCCCGTGCCGAGCTCGAAGTCACCGTGCGCCAACAGCGCCAGGCCTACAACGCGCTGG > ACB122/3210756‑3211009 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |