| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,626,106 | 0 | T | G | 52.9% | 3.3 / 12.8 | 17 | V174G (GTG→GGG) | hcf136_3 | Ycf48‑like protein |
| Reads supporting (aligned to +/- strand): ref base T (7/1); new base G (1/8); total (8/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.37e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.66e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCAT‑GCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCG > ACB122/3625975‑3626228 | agCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCCCCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGc > 2:66818/1‑135 (MQ=255) aGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCAT‑gc > 8:96882/1‑135 (MQ=255) aGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGCATTTCACCGATGCCCGCCACGGCCTGGTCGTCGGGGGCGACGGGGGGGGCCT‑GCGCCccgccgc > 2:46406/1‑134 (MQ=255) aGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCAT‑GCGCCCcgacgc > 2:20773/1‑134 (MQ=255) aGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCAT‑GCGCACcgccga > 3:160073/1‑135 (MQ=255) cgcAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCAT‑GCGCCCCGCCGCCGGCGGGCGCAGCGGGCAGTc > 3:195304/1‑133 (MQ=255) aCAAGCCGTTGGGGGCCCTCAATATCACCGATCCCCGCACCGGCGTGGTCGTCGGGGCCTACGGGGGGGCCAT‑GCGCACCTACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTg < 4:160073/135‑1 (MQ=255) cGTTGTGGGCCCTTAATTTCCCCGAGCCCCCCACCGGCGGGGGCGGGGGGGCCTCGGGGGGGGCCTG‑GCGCCCCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTg < 8:91939/134‑1 (MQ=255) cTGAATTTCACCGCTTCCCGCAACGGCCTGGTCGTCGGTGCCAAAGGGGTGGCCAT‑GCGCACCGCCGCCGGCGGGCGCCGCTGGCAGTCGGAGATCGGCCGACAGCCGCACGGGCGTGGACTGCACCTGTATGc > 8:168285/1‑134 (MQ=255) cgccgCCCGCACGGGGCGGGGCGGCGGGGCCT‑CGGGGGGGCCCTGGCCCACGGCCGACGGCGGGCGCAGCTGGCAGTCGGAGAGCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCa < 5:141583/131‑1 (MQ=255) ttGCCCGCACCGGGCGGGGCGGGGGGGCCTCGGGGGGGCCCAG‑GCCCCCCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAg < 1:20773/134‑1 (MQ=255) tGCCCGCACCGGCCTGGGCGTCGGTGCCTACGGGGGGGCCAT‑GCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCATGGCGCGCCAAgg < 4:57593/135‑1 (MQ=255) cccGCCACGGCCTGGTGGTGGGTGCCTCCGGGGTGCACTC‑GCGCCCCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCa < 2:166006/135‑1 (MQ=255) cccGCACCGGCGTGGGCGCGGGGGCCTACGGGGGGGCCAG‑GCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCa < 4:85106/135‑1 (MQ=255) cGCACCGGCCGGGGCGGCGGGGCCCACGGGGGGGCCAG‑CCGCCCCGCCGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAAc < 7:63636/135‑1 (MQ=255) tcgGTGCCTACGGGGTGGCCAA‑TCGCACCCACGCCCGCGGGCGCCGCTGGCACTCGGAGATAGGCCCACAGCCGAACCGGCGGGGACTGCACCAGTATTCCCCGGCCCGCCCACGCCACCCCGTGTACAACGccc > 8:83026/1‑134 (MQ=255) gggCCTCCGGGGGGCCCAG‑GCCCCCCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCg < 1:66818/133‑1 (MQ=255) | AGCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCAT‑GCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCG > ACB122/3625975‑3626228 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |