Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB122	179,484	T→C	C316R (TGC→CGC)	CJ019_00182 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	179,484	0	T	C	100.0%	103.2 / NA	29	C316R (TGC→CGC)	CJ019_00182	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/19);  total (10/19)

TCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCTGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGTG  >  ACB122/179360‑179615                                                                                                                             |                                                                                                                                    tCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCgcgg                                                                                                                           >  1:71458/1‑135 (MQ=255) tCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCgcgg                                                                                                                           >  3:78984/1‑135 (MQ=255)          ggcgggcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGtt                                                                                                                  <  7:155758/135‑1 (MQ=255)            ctggcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGtt                                                                                                                  <  2:188802/133‑1 (MQ=255)            cgggcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGtt                                                                                                                  <  2:93095/131‑1 (MQ=255)             tggcCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGttt                                                                                                                 >  8:202598/1‑133 (MQ=255)                ccggAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGt                                                                                                            <  3:78193/132‑1 (MQ=255)                     aCGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCt                                                                                                         <  4:84796/133‑1 (MQ=255)                           gccTTGGCGGCCAGCGTACCGACCAGGTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCCGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCgt                                                                                                 <  7:98866/135‑2 (MQ=255)                            ccTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCggg                                                                                                <  7:69071/135‑1 (MQ=255)                                  cggcCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGt                                                                                          >  3:67037/1‑135 (MQ=255)                                       aGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATg                                                                                     <  4:72582/135‑1 (MQ=255)                                        gCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATga                                                                                    <  6:113794/135‑1 (MQ=255)                                              cGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGc                                                                              <  7:32699/135‑1 (MQ=255)                                                                  tAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGctc                                                          >  6:113745/1‑135 (MQ=255)                                                                        aaCAGCGGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAgg                                                    <  8:181574/135‑1 (MQ=255)                                                                             cTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCGGGAGGCACCg                                               >  5:172430/1‑135 (MQ=255)                                                                                 gtcgtACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGtt                                             <  6:167418/133‑1 (MQ=255)                                                                                        ttGTGCCAGCTACCCGGATACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCt                                    <  2:63709/135‑1 (MQ=255)                                                                                        ttGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCt                                    <  4:78984/135‑1 (MQ=255)                                                                                        ttGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCt                                    <  2:71458/135‑1 (MQ=255)                                                                                                gCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGCgg                                                    >  4:34705/1‑111 (MQ=255)                                                                                                gCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAgg                                                    <  3:34705/111‑1 (MQ=255)                                                                                                gCTACCCGGAAACCAACGACCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGaaa                            >  5:165161/1‑135 (MQ=255)                                                                                                      cGGAAACCACCGACCTCATTTCCCGCAGCGGCGGCGGCGAGTTTGCCGTGCCTGCACCGGGCATGGTGCATGAGGAGTCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACggggg                      <  6:14357/135‑1 (MQ=255)                                                                                                                  aCCTCATTTCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGAAGTACACCTTGCCAAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACCGGGGCCAGTGATATCg          >  8:77624/1‑135 (MQ=255)                                                                                                                         ttCCCGCCGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt   <  6:197464/135‑1 (MQ=255)                                                                                                                         ttCCCGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGt   <  4:66885/135‑1 (MQ=255)                                                                                                                          tCCCGCAGCCGCGGCGCCGAGTTTGCAGTGCTGGCACCGGCCAAGGAGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGACACGGGGGCCAGTGATATCGCCCCGGTg  >  2:80718/1‑135 (MQ=255)                                                                                                                             |                                                                                                                                    TCAGGGCCTGGCTGGCCTGAACGCCCGCCTTGGCGGCCAGCGTACCGACCAGTTGTTGCAAGCGGTAGGTGAACAGCTGCGTCGTACTTGTGCCAGCTACCCGGAAACCAACGACCTCATTTCCTGCAGCCGCGGCGGCGAGTTTGCAGTGCTGGCACCGGGCATGGTGCATGAGGAGGCAGTACACCTTGCCCAGGCTCTGGAGGCAACGTTGCAGAGCCTGCACGAAACGGGGGCCAGTGATATCGACCCGGTG  >  ACB122/179360‑179615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑