Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB122	1,527,131	G→A	A696T (GCC→ACC)	secA →	Protein translocase subunit SecA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	1,527,131	0	G	A	100.0%	112.9 / NA	34	A696T (GCC→ACC)	secA	Protein translocase subunit SecA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/16);  total (18/16)

CGAGAAAGCCCAGCGCAAGGTCGAAGGCCGCAACTTCGACATTCGTAAGCAATTGCTCGAATATGACGACGTCGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGGCCGCCGAGAACATTGGCGACACCATCGTCGAATTCCGCAAGGAAGTACTGGACGCTACCATCAGCCAGCACATTCCGCCGCAGTCGCTGCCAGAGCAGTGGGACGTAGCCGGCCTGGAGGCTTCGCTGGC  >  ACB122/1527010‑1527261                                                                                                                          |                                                                                                                                   cGAGAAAGCCCAGCGCAAGGTCGAAGGCCGCAACTTCGACATTCGTAAGCAATTGCTCGAATATGACGACGTCGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACAt                                                                                                                       >  2:47560/1‑135 (MQ=255)        gCCCAGCGCAAGGTCGAAGGCCGCAACTTCGACATTCGTAAGCAATTGCTCGAATATGACGACGTCGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGac                                                                                                                >  6:203568/1‑135 (MQ=255)                cAAGGTCGAAGGCCGCAACTTCGACATTCGTAAGCAATTGCTCGAATATGACGACGTCGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCAtcgt                                                                                                        >  7:200519/1‑135 (MQ=255)                 aaGGTCGAAGGCCGCAACTTCGACATTCGTAAGCAATTGCTCGAATATGACGACGTCGCCAACGAGCAGCTCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCAtcgtc                                                                                                       <  5:89199/135‑1 (MQ=255)                 aaGGTCGAAGGCCGCAACTTCGACATTCGTAAGCAATTGCTCGAATATGACGACGTCGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCAtcgt                                                                                                        >  8:103906/1‑134 (MQ=255)                      cGAAGGCCGCAACTTCGACATTCGTAAGCAATTGCTCGAATATGACGACGTCGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCATCGTCGAAtt                                                                                                  >  7:54779/1‑135 (MQ=255)                        aaGGCCGCAACTTCGACATTCGTAAGCAATTGCTCGAATATGACGACGTCGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGTGAACATTGGCGACACCATCGTCGAATTcc                                                                                                <  1:115036/135‑1 (MQ=255)                        aaGGCCGCAACTTCGACATTCGTAAGCAATTGCTCGAATATGACGACGTCGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCATCGTCGAATTcc                                                                                                <  3:12993/135‑1 (MQ=255)                           gCCGCAACTTCGACATTCGTAAGCAATTGCTCGAATATGACGACGTCGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCATCGTCGAATTCCGCa                                                                                             <  3:199122/135‑1 (MQ=255)                           gCCGCAACTTCGACATTCGTAAGCAATTGCTCGAATATGACGACGGCGCCAACGAGCAGCTCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCATCGTCGAATTCCGCa                                                                                             <  5:39098/135‑1 (MQ=255)                                  cTTCGACATTCGTAAGCAATTGCTCGAATATGACGACGTCGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGaca                                                                                                               >  3:85643/1‑110 (MQ=255)                                  cTTCGACATTCGTAAGCAATTGCTCGAATATGACGACGTCGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGaca                                                                                                               <  4:85643/110‑1 (MQ=255)                                  cTTCGACATTCGTAAGCAATTGCTCGAATATGACGACGTCGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCATCGTCGAATTCCGCAAGGAAGt                                                                                      >  6:187245/1‑135 (MQ=255)                                   ttCGACATTCGTAAGCAATTGCTCGAATATGACGACGTCGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCATCGTCGAATTCCGCAAGGAAGTa                                                                                     <  1:47560/135‑1 (MQ=255)                                      gACATTCGTAAGCAATTGCTCGAATATGACGACGTCGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCATCGTCGAATTCCGCAAGGAAGTAc                                                                                    <  4:61015/133‑1 (MQ=255)                                               aaGCAATTGCTCGAATATGACGACGTCGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCATCGTCGAATTCCGCAAGGAAGTACTGGACGCTAcc                                                                         >  7:125895/1‑135 (MQ=255)                                                aGCAATTGCTCGAATATGACGACGTCGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCATCGTCGAATTCCGCAAGGAAGt                                                                                      >  1:13447/1‑121 (MQ=255)                                                aGCAATTGCTCGAATATGACGACGTCGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCATCGTCGAATTCCGCAAGGAAGt                                                                                      <  2:13447/121‑1 (MQ=255)                                                  cAATTGCTCGAATATGACGACGTCGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCATCGTCGAATTCCGCAAGGAAGTACTGGACGCTACCATc                                                                      <  6:191837/135‑1 (MQ=255)                                                        cTCGAATATGACGACGTCGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCATCGTCGAATTCCGCAAGGaa                                                                                        >  8:190815/1‑111 (MQ=255)                                                        cTCGAATATGACGACGTCGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCATCGTCGAATTCCGCAAGGaa                                                                                        <  7:190815/111‑1 (MQ=255)                                                          cGAATATGACGACGTCGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCATCGTCGAATTCCGCAAGGAAGTACTGGACGCTACCATcagccagc                                                               >  8:35326/1‑134 (MQ=255)                                                                         cGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCATCGTCGAATTCCGCAAGGAAGTACTGGACGCTACCATCAGCCAGCACATTCCGCCGCAGTc                                               >  4:165723/1‑135 (MQ=255)                                                                           ccAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCATCGTCGAATTCCGCAAGGAAGTACTGGACGCTACCATCAGCCAGCACATTCCGCCGCAGTCGc                                             <  7:35326/135‑1 (MQ=255)                                                                            cAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCATCGTCGAATTCCGCAAGGAAGTACTGGACGCTACCATCAGCCAGCACATTCCGCCGCAGTCGCt                                            >  5:73188/1‑135 (MQ=255)                                                                                    agcGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCATCGTCGAATTCCGCAAGGAAGTACTGGACGCTACCATCAGCCAGCACATTCCGCCGCAGTCGCTGCCAGAGc                                    <  1:190170/135‑1 (MQ=255)                                                                                        cAAGGTGAGCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCCTCGTCGAATTCCGCAAGGAAGTACTGGACGCTACCATCAGCCAGCACATTCCGCCGCAGTCGCTGCCAGAGCAGTg                                <  3:153589/135‑1 (MQ=255)                                                                                             tGATCTACCACATGCGCAACAGCCTGCGGACCGCCGAGAACATTGGCGACACCATCGTCGAATTCCGCAAGGAAGTACTGGACGCTACCATCAGCCAGCACATTCCGCCGCAGTCGCTGCCAGAGCAGTGGGACg                           <  2:185292/135‑1 (MQ=255)                                                                                              gATCTACCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCATCGTCGAATTCCGCAAGGAAGTACTGGACGCTACCATCAGCCAGCACATTCCGCCGCAGTCGCTGCCAGAGCAGTGGGACGt                          <  5:187245/135‑1 (MQ=255)                                                                                                   aCCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCATCGTCGAATTCCGCAAGGAAGTACTGGACGCTACCATCAGCCAGCACATTCCGCCGCAGTCGCTGCCAGAGCAGTGGGACGTAGCCg                     >  3:150373/1‑135 (MQ=255)                                                                                                   aCCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCATCGTCGAATTCCGCAAGGAAGTACTGGACGCTACCATCAGCCAGCACATTCCGCCGCAGTCGCTGCCAGAGCAGTGGGACGTAGCCg                     >  6:45024/1‑135 (MQ=255)                                                                                                   aCCACATGCGCAACAGCCTGCTGACCGCCGAGAACATTGGCGACACCATCGTCGAATTCCGCAAGGAAGTACTGGACGCTACCATCAGCCAGCACATTCCGCCGCAGTCGCTGCCAGAGCAGTGGGACGTAGCCg                     >  4:47257/1‑135 (MQ=255)                                                                                                            gcAACAGCCTGCTGACCGACGAGAACATTGGCGACACCATCGTCGAATTCCGCAAGGAAGTACTGGACGCTACCATCAGCCAGCACATTCCGCCGCAGTCCCTGCCAGAGCAGTGGGACGTAGCCGGCCTGGAgg            >  6:64931/1‑135 (MQ=255)                                                                                                                      gctgACCGCCGAGAACATTGGCGACACCATCGTCGAATTCCGCAAGGAAGTACTGGACGCTACCATCAGCCAGCACATTCCGCCGCAGTCGCTGCCAGAGCAGTGGGACGTAGCCGGCCTGGAGGCTTCGCTGGc  >  7:128888/1‑135 (MQ=255)                                                                                                                          |                                                                                                                                   CGAGAAAGCCCAGCGCAAGGTCGAAGGCCGCAACTTCGACATTCGTAAGCAATTGCTCGAATATGACGACGTCGCCAACGAGCAGCGCAAGGTGATCTACCACATGCGCAACAGCCTGCTGGCCGCCGAGAACATTGGCGACACCATCGTCGAATTCCGCAAGGAAGTACTGGACGCTACCATCAGCCAGCACATTCCGCCGCAGTCGCTGCCAGAGCAGTGGGACGTAGCCGGCCTGGAGGCTTCGCTGGC  >  ACB122/1527010‑1527261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑