| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB122 | 3,065,508 | G→A | V53M (GTG→ATG) | rcsC_6 → | Sensor histidine kinase RcsC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 3,065,508 | 0 | G | A | 95.0% | 57.1 / NA | 20 | V53M (GTG→ATG) | rcsC_6 | Sensor histidine kinase RcsC |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (9/10); minor base C (1/0); total (10/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGCGGGCGTTGGCCGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCGTGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGG > ACB122/3065376‑3065625 | ccgcGGGCGTTGGCCGGGCTGCTGGGGCGGGGTGCCCACTCGGCGCGGAAAAGCCAGTACCCGGCGCTGTCCGCCCGCCTGGCCGAACTGGCAGCCGAACGCCACCGTGACAAATGGCTGGTCGCAAACGCCCTg > 6:198845/3‑135 (MQ=255) gggCGGTGGCGGGGCGGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTCCCCGGAGCTGGCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACg < 5:198845/135‑1 (MQ=255) cGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCa < 5:184957/135‑1 (MQ=255) cGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCa < 8:40766/135‑1 (MQ=255) ggCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAgg < 2:95777/135‑1 (MQ=255) ctggggctgggTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGc < 5:50118/135‑1 (MQ=255) ggctgggTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGCCGAACTGGCAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCCCGGTATTTTCCAGGCCCGCCt > 6:124086/1‑133 (MQ=255) ggTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTGACAAATGGCTGTTCGAAAACGCCATGCAAGGTATTTTCCAGGCCAGCCTTCAGGAc > 2:111707/1‑135 (MQ=255) cTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACTCAATGCACGCTATTTTCCAGGCCAGCCTTCAGGACGGCATGCg > 7:175158/1‑135 (MQ=255) cgcgTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTgccg > 8:180277/1‑135 (MQ=255) aaaaGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAgg < 5:140834/103‑1 (MQ=255) aaaaGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAgg > 6:140834/1‑103 (MQ=255) ttACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCt > 1:64372/1‑135 (MQ=255) gAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGgcgcgc < 4:203762/135‑1 (MQ=255) gCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCAt < 4:179622/135‑1 (MQ=255) gcccgccTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCgg > 3:3925/1‑134 (MQ=255) gcccgccTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCgg > 1:129396/1‑134 (MQ=255) aaCTGGAACCCGAACGCAACCGTTACAAATGGCTGTTCGAAACCGCCATGCACGGTATTTTCCAGGCCAGCCTTCTGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCGCGGCTATGACGACCCGc < 1:205010/135‑1 (MQ=255) aaaTGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCctgactga < 7:182747/134‑1 (MQ=255) ggCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTgg > 4:156442/1‑135 (MQ=255) | CAGCGGGCGTTGGCCGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCGTGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGG > ACB122/3065376‑3065625 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |