Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB122	3,957,240	C→T	G137R (GGG→AGG)	dmlR_13 ←	HTH‑type transcriptional regulator DmlR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	3,957,240	0	C	T	100.0%	153.2 / NA	45	G137R (GGG→AGG)	dmlR_13	HTH‑type transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/22);  total (23/22)

CTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGC  >  ACB122/3957112‑3957367                                                                                                                                 |                                                                                                                                cTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGAGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGc                                                                                                                           <  3:154777/135‑1 (MQ=255)      gTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCg                                                                                                                      <  2:129432/135‑1 (MQ=255)            cGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCCCCCCGTCATGCCTGTCGGCCAGCGGGTCgg                                                                                                                >  1:97462/1‑135 (MQ=255)            cGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCgg                                                                                                                <  5:57923/135‑1 (MQ=255)               gggTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGCTCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGt                                                                                                             >  3:137705/1‑135 (MQ=255)                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGCTCAGTTGCGACTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                           >  5:10297/1‑135 (MQ=255)                 gtgtCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTg                                                                                                           >  8:36868/1‑135 (MQ=255)                 gtgtCCAGGTAGCCGGGGGCCGCGCACGGGATGCTCAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATCCCTTTCGCCCAGCGGGTCGGGGATg                                                                                                           >  2:19705/1‑135 (MQ=255)                         gTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGa                                                                                                   >  5:57001/1‑135 (MQ=255)                                    ccGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCt                                                                                        >  2:55615/1‑135 (MQ=255)                                        gcAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTGCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGAt                                                                                    <  7:34865/135‑1 (MQ=255)                                             ggAGGCTGAAAATATTGCCCAGCGGCACAGCGAGCTGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGGCGGGGTTGCGCTGCGACAGCGTCAGCTCGATcacca                                                                               <  6:6599/135‑1 (MQ=255)                                               aTGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGACTATCTGCCAGCCGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCCCTcaccccg                                                                             >  8:163775/1‑132 (MQ=255)                                                   tGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCg                                                                         <  6:52212/135‑1 (MQ=255)                                                                      cacaGCGATCAGTTGCGACTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGcc                                                      <  7:38817/135‑1 (MQ=255)                                                                                aGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGc                                            >  6:672/1‑135 (MQ=255)                                                                                aGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGc                                            >  7:140399/1‑135 (MQ=255)                                                                                aGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGc                                            >  1:29041/1‑135 (MQ=255)                                                                                  ttGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCgg                                          <  5:672/135‑1 (MQ=255)                                                                                   tGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGc                                         <  4:77504/135‑1 (MQ=255)                                                                                   tGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGc                                         >  3:6585/1‑135 (MQ=255)                                                                                   tGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGc                                         <  5:111154/135‑1 (MQ=255)                                                                                   tGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGc                                         <  6:149697/135‑1 (MQ=255)                                                                                        aTCCGGCAGCGCCCCGTCCACGGGGATCACGACGTCAGGCCTTTCGGCCAGGGGGGCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                    <  2:190080/135‑1 (MQ=255)                                                                                        aTCCGGCAGCGCACCGTCCTCGGTGATCACCACGTCATGCCTTTCGGCCAGAGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTGGTAGCCGGCCACCAGCGGCATCAg                                    <  7:163775/135‑1 (MQ=255)                                                                                        aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCAGCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                    <  1:19705/135‑1 (MQ=255)                                                                                        aTCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGGCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                    <  6:147606/135‑1 (MQ=255)                                                                                        aTCCGGCAGCGCACCGTCCACGGTGATCACCACCTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                    <  6:12245/135‑1 (MQ=255)                                                                                        aTCCGGCAGCGCACCGTCCAAGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCTTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAg                                    <  4:28562/135‑1 (MQ=255)                                                                                          ccGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGCTCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCa                                  >  6:43716/1‑135 (MQ=255)                                                                                          ccGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCa                                  >  3:60459/1‑135 (MQ=255)                                                                                          ccGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCa                                  >  7:29853/1‑135 (MQ=255)                                                                                               aGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGAt                                                                                    <  3:20967/80‑1 (MQ=255)                                                                                               aGCGCACCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGAt                                                                                    >  4:20967/1‑80 (MQ=255)                                                                                                    aCCGTCCACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCAt                        >  3:36358/1‑135 (MQ=255)                                                                                                         ccACGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGt                   >  8:87109/1‑135 (MQ=255)                                                                                                            cGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg                <  7:137285/135‑1 (MQ=255)                                                                                                            cGGTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGGCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCg                <  1:118074/135‑1 (MQ=255)                                                                                                              gTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCgg               >  7:16761/1‑134 (MQ=255)                                                                                                              gTGATCACCACGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCgg               >  7:13606/1‑134 (MQ=255)                                                                                                                     ccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTc                                                                                            >  2:196017/1‑50 (MQ=255)                                                                                                                     ccacGTCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTc                                                                                            <  1:196017/50‑1 (MQ=255)                                                                                                                          tCATGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGc  <  6:57001/135‑1 (MQ=255)                                                                                                                            aTGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAAc    >  2:66863/1‑131 (MQ=255)                                                                                                                            aTGCCTTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAAc    >  8:2143/1‑131 (MQ=255)                                                                                                                                 |                                                                                                                                CTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGCGATCAGTTGCGAATCCGGCAGCGCACCGTCCACGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGC  >  ACB122/3957112‑3957367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑