| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB122 | 4,612,646 | (A)10→11 | intergenic (+1098/‑681) | CJ019_04358 → / → rhsC | hypothetical protein/Putative deoxyribonuclease RhsC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 4,612,636 | 1 | . | A | 80.0% | 109.7 / 24.7 | 40 | intergenic (+1088/‑691) | CJ019_04358/rhsC | hypothetical protein/Putative deoxyribonuclease RhsC |
| Reads supporting (aligned to +/- strand): ref base . (3/5); new base A (14/18); total (17/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCTTCCAAGAAGCGAAGTTAGAGCGAATTATTTAAAATAAGCAAACAATAAAGCCCAAGCAAAACTCACACAAACTCCCGTAAAACTCAGAAGTGGAACATGCAAATGAACAAAGCTAACATCTGGAGATG‑GTC‑C‑‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCACCCAGGAAGACGGAACATATTATTTTGGGCAAATAATATCAAAAATAATCACTGGCCATGTAGCTGAAAT > ACB122/4612501‑4612764 | aCCTTCCAAGAAGCGAAGTTAGAGCGAATTATTTAAAATAAGCAAACAATAAAGCCCAAGCAAAACTCACACAAACTCCCGTAAAACTCAGAAGTGGAACATGCAAATGAACAAAGCTAACATCTGGAGATG‑GTC‑C‑‑a > 7:172474/1‑137 (MQ=255)aCCTTCCAAGAAGCGAAGTTAGAGCGAATTATTTAAAATAAGCAAACAATAAAGCCCAAGCAAAACTCACACAAACTCCCGTAAAACTCAGAAGTGGAACATGCAAATGAACAAAGCTAACATCTGGAGATG‑GTC‑C‑‑a > 1:46458/1‑137 (MQ=255) ttCCAAGAAGCGAAGTTAGAGCGAATTATTTAAAATAAGCAAACAATAAAGCCCAAGCAAAACTCACACAAACTCCCGTAAAACTCAGAAGTGGAACATGCAAATGAACAAAGCTAACATCTGGAGATG‑GTC‑C‑‑aaaa < 2:120395/137‑1 (MQ=255) ttCCAAGAAGCGAAGTTAGAGCGAATTATTTAAAATAAGCAAACAATAAAGCCCAAGCAAAACTCACACAAACTCCCGTAAAACTCAGAAGTGGAACATGCAAATGAACAAAGCTAACATCTGGAGATG‑GTC‑C‑‑aaaa > 5:45930/1‑137 (MQ=255) ttCCAAGAAGCGAAGTTAGAGCGAATTATTTAAAATAAGCAAACAATAAAGCCCAAGCAAAACTCACACAAACTCCCGTAAAACTCAGAAGTGGAACATGCAAATGAACAAAGCTAACATCTGGAGATG‑GTC‑C‑‑aaaa > 5:41138/1‑137 (MQ=255) agaagCGAAGTTAGAGCGAATTATTTAAAATAAGCAAACAATAAAGCCCAAGCAAAACTCACACAAACTCCCGTAAAACTCAGAAGTGGAACATGCAAATGAACAAAGCTAACATCTGGAGATG‑GTC‑C‑‑aaaaaaaaa > 6:157315/1‑137 (MQ=255) agCGAAGTTAGAGCGAATTATTTAAAATAAGCAAAAAATAAAGCCCAAGCAAAACTCACACAAACTCCCGTAAAACTCAGAATTGGAACATGCAAATTAACAAAGCTAACATCTGGAGATT‑GGC‑C‑‑aaaaaaaaaaaa < 6:166949/137‑3 (MQ=255) cGAAGTTATAGCGAATTATTTAAAATAAGCAAACAATAAAGCCCAAGCAAAACTAACACAAACTCCCGAAAAACACACAAGGGGAACATGCAAATGAACAAAGCTAACATCTGGAGATG‑GTC‑C‑‑aaaaaaaaaaacaa < 1:198167/137‑5 (MQ=255) aGTTAGAGCGAATTATTTAAAATAAGCAAACAATAAAGCCCAAGCAAAACTCACACAAACTCCCGTAAAACTCAGAAGTGGAACATGCAAATGAACAAAGCTAACATCTGGAGATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGa > 1:162495/1‑137 (MQ=255) aGTTAGAGCGAATTATTTAAAATAAGCAAACAATAAAGCCCAAGCAAAACTCACACAAACTCCCGTAAAACTCAGAAGTGGAACATGCAAATGAACAAAGCTAACATCTGGAGATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGa > 8:332688/1‑137 (MQ=255) ttAGATCGAATTATTTAAAATAAGAAAACAATAAAGCCCAAGCAAAACTCACACAAACTCCCGTAAAACTCAGAAGTGGAACATGCAAATGAACAAACCTAACATCTGGAGATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGACt < 6:164071/137‑1 (MQ=255) ttAGAGCGAATTATTTAAAATAAGCAAACAATAAAGCCCAAGCAAAACTCACACAAACTCCCGTAAAACTCAGAAGTGGAACATGCAAATGAACAAAGCTAACATCTGGAGATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGAca > 3:254894/1‑136 (MQ=255) acctaaTTATTTAAAAAAACCAAAAAATAAACCCCAACAAAAACTCACACAAACTCCCGTAAAACTCAGAAGTGGAAAATGCAAATGAACAAACCAAACATCTGGAGATG‑GTC‑CAAAAAAAAAAAAC‑AAAGACTACa < 2:186977/133‑1 (MQ=255) aaaTTATTTAAAATAAGCAAACAATAAAGCACAAGCAAAACTCACACAAACTCCCGTAAAACTCAGAAGTGGAACATGAAAATGAAAAAAGCTAACATGTGGAGATG‑GTC‑CAAAAAAAAAAAAC‑AAAGACTACATTa < 5:280581/136‑1 (MQ=255) aaaTAAGCAAACAATAAAGCCCAAGCAAAAATCACACAAACTCCCTTAAAACTCCTAAGTGGAACATGCAAATGAAAAAATCTAACATCTGGAGATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATaaa < 7:181962/137‑1 (MQ=255) aTAAGCAAACAATAAAGCCCAAGCAAAACTCACACAAACTCCCGTAAAACTCAGAAGTGGAACATGCAAATGAACAAAGCTAACATCTGGAGATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAg < 5:233587/137‑1 (MQ=255) aTAAGCAAACAATAAAGCCCAACCAAAACTCACACAAACTCCCGTAAAACTCAGAATTGGAACATGCAAATGAAAAAAGCTAACATCTGGAGATG‑GTCAA‑‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAg < 7:351590/137‑1 (MQ=255) aaGCAAACAATAAATCCCAAGCAAAACTCACACAAACTCCCGTAAAACTCAGAAGTGGACCATGCAAATGAACAAAGATAAAATCTTGAGATG‑GTC‑C‑‑AAAAAAAAAACAAAACACTACAATAAAGAACAAAAAAGCa > 6:208953/1‑137 (MQ=255) aaaCAATAAAGCCCAAGCAAAACTCACACAAACTCCCGTAAAACTCAGAAGTGGAACATGCAAATGAACAAAGCTAACATCTGGAGATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGgat > 5:170189/1‑136 (MQ=255) aGCCCAAGCAAAACTCAAAAAAACTCCCGTAAAACTCACAATTGTAACATGCAAATGAACAAAGCTAACATCTGGAGATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCt < 6:96041/137‑1 (MQ=255) ccAAGCAAAACTCACACAAACTCCCGTAAAACTCAGAAGTGGAACATGCAAATGAACAAAGCTAACATCTGGAGATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCt > 7:203721/1‑137 (MQ=255) ccAAGCAAAACTCACACAAACTCCCGTAAAACTCAGAAGTGGAACATGCAAATGAACAAAGCTAACATCTGGAGATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCt < 2:22597/137‑1 (MQ=255) cAAAACTCACACAAACTCCCGTAAAACTCAGAAGTGGAACATGCAAATGAACAAAGCTAACATCTGGAGATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCAcc > 5:226895/1‑137 (MQ=255) cAAAACTCACACAAACTCCCGTAAAACTCAGAAGTGGAACATGCAAATGAACAAAGCTAACATCTGGAGATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGAAAATTTCTACATCAcc > 1:105153/1‑137 (MQ=255) acacacaAACTCCCGTAAAACTCATAAGTGGAACATGCAAATTAAAAAAGCTAACATCTGGAGATG‑GTC‑CAAAAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCACCCAGGa < 8:172474/136‑1 (MQ=255) cacacaAACTCCCGTAAAACTCAGAAGTGGAACATGCAAATGAACAAAGCTAACATCTGGAGATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCACCCAGGAAg > 7:375761/1‑137 (MQ=255) acaaaaaaCTCACTTAAAACTAATCATTGTAACATTCAAATTAACAAAGTTAAAATGTTGAGTTGTGCA‑A‑‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCACCCAGGAAg < 5:208953/132‑1 (MQ=255) cacaAACTCCCGTAAAACTCAGAAGTGGAACATGCAAATGAACAAAGCTAACATCTGGAGATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCACCCAGGAAGAc < 7:285439/137‑1 (MQ=255) aaaaCACCCGTAAAACTCACAAGTGGAACATGCAAATGAACAAAGCTAACATCTGGACATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCACCCAGGAAGACgg < 6:124514/136‑1 (MQ=255) cGTAAAACTCAGAAGTGGAACATGCAAATGAACAAAGCTAACATCTGGAGATG‑GTCAA‑‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCACCCAGGAAGACGGAACAtatt < 3:164413/137‑1 (MQ=255) aGAAGTGGAACATGCAAATGAACAAAGCTAACATCTGGAGATG‑GTC‑C‑‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCACCCAGGAAGACGGAACATATTATTTTGGGCaa > 5:303628/1‑137 (MQ=255) aTGCAAATGAACAAAGCTAACATCTGGAGATG‑GTC‑C‑‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCACCCAGGAAGACGGAACATATTATTTTGGGCAAATAATATCaaa < 7:64279/137‑1 (MQ=255) aTGCAAAAGAACAAAGCTAACAGATGTACATG‑GAC‑CA‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCACCCAGGAAGACGGAACATATTATTTTGGGCAAATAATATCaa < 8:340827/137‑1 (MQ=255) cAAATGAACAAAGCTAACATCTGGAGATG‑GTC‑C‑‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCACCCAGGAAGACGGAACATATTATTTTGGGCAAATAATATCAAAaat < 6:139868/137‑1 (MQ=255) aaaTGAACAAAGCTAACATCTGGAGATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTATGCTTCACCCAGGAAGACGGAACATATTATTTTGGGAAAATAATATAAAAaat > 4:206820/1‑137 (MQ=255) tGAACAAAGCTAACATCTGGAGATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCACTCAGGAAGACGGAACATATTATTTTGGGCAAATAATATCAAAaataat < 8:186137/137‑1 (MQ=255) aCAAAGCTAACATCTGGAGATG‑GTC‑CAAAAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCACCCAGGAAGACGGAACATATTATTTTGGGCAAATAATATAAAAAATAATCa > 6:260917/1‑137 (MQ=255) aGCTAACATCTGGAGATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCACCCAGGAAGACGGAACATATTATTTTGGGCAaataa > 3:104379/1‑117 (MQ=255) aGCTAACATCTGGAGATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCACCCAGGAAGACGGAACATATTATTTTGGGCAaataa < 4:104379/117‑1 (MQ=255) aGCTAACATCTGGAGATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCACCCAGGAAGACGGAACATATTATTTTGGGCAAATAATATCAAAAATAATCACTGGc > 5:354985/1‑137 (MQ=255) tAACATCTGGAGATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGTGATATTTTCTGCTTCACCCAGGAAGACGGAACATATTAGTTTGGGCAAATAATATAAAAAATAATCACTGGCCAt > 1:327065/1‑137 (MQ=255) tAACATCTGGAGATG‑GTC‑C‑‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCACCCAGGAAGACGGAACATATTATTTTGGGCAAATAATATCAAAAATAATCACTGGCCATg > 5:373597/1‑137 (MQ=255) aaCATCTGGAGATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCACCCAGGAAGACGGAACATATTATTTTGGGCAAATAATATCAAAAATAATCACTGGCCATg < 5:157315/137‑1 (MQ=255) aTCTGGAGATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCACCCAGGAAGACGGAACATATTATTTTGGGCAAATAATATCAAAAATAATCACTGGCCATGTAg < 8:96393/137‑1 (MQ=255) cTGGAGATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCACCCAGGAAGACGGAACATATTATTTTGGGCAAATAATATCAAAAATAATCACTGGCCATGTAGCt < 6:6209/137‑1 (MQ=255) cTGGAGATG‑GTC‑CA‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCACCCAGGAAGACGGAACATATTATTTTGGGCAAATAATATCAAAAATAATCACTGGCCATGTAGCt < 8:375761/137‑1 (MQ=255) agaTG‑GTC‑CA‑AAAAAAAAAAC‑AAAGCCGACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCACCCAGGAAGACGGAACATATTATTTTGGGCAAATAATATCAAAAATAATCAATGGCAATGTAGCTGaaa > 2:30642/1‑137 (MQ=255) taTG‑GTC‑CA‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCACCCAGGAAGACGGAACATATTATTTTGGGCAAATAATATCAAAAATAATCACTGGCCATGTAGCTGAAat < 1:59293/136‑1 (MQ=255) | ACCTTCCAAGAAGCGAAGTTAGAGCGAATTATTTAAAATAAGCAAACAATAAAGCCCAAGCAAAACTCACACAAACTCCCGTAAAACTCAGAAGTGGAACATGCAAATGAACAAAGCTAACATCTGGAGATG‑GTC‑C‑‑AAAAAAAAAAC‑AAAGACTACATTAAAGAACATAAAAGCAGGAGATATTTTCTGCTTCACCCAGGAAGACGGAACATATTATTTTGGGCAAATAATATCAAAAATAATCACTGGCCATGTAGCTGAAAT > ACB122/4612501‑4612764 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |