| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 434,376 | 0 | G | . | 22.7% | 48.5 / 13.2 | 22 | coding (207/1173 nt) | bioF | 8‑amino‑7‑oxononanoate synthase |
| Reads supporting (aligned to +/- strand): ref base G (8/9); new base . (3/2); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.58e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGCCATCA > ACB122/434245‑434512 | aGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCCGCGGTTGGTGGGCTTGTGCAGCAATGACTACCTGGGCCTGGCCCACCACCCCCAGGTGATCGCCGCCTGGCAGGCCGGCGCCGCGCGCGGGGGTGTgggggg > 2:1628/1‑137 (MQ=255) gAAGTGGTGGTCGACGGCCAGCGGTTGGTGGGCGTCTGCCGCAATGACTACCTGGGCCTGGCCAACCCCCCCGAGGTGATAGCCGCCTGGCCGGCCGGCGCCGCGCGCGGGGGTGTGGGGGGGGGGGCCGCGccccc > 2:10563/1‑136 (MQ=255) aGTGGTGGTCGACGGCCCGCGGTTGCTGGCCGTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCCCGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGt > 6:58121/1‑137 (MQ=255) ggTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCGTGGCAGGCGGGCGCCGAGCGCTGGGGTGT‑‑GGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCTCa < 6:172463/137‑1 (MQ=255) ggCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACGTGGTAGTTGGCCACCGCACCCCGCACCATc > 1:121336/1‑137 (MQ=255) tCTGCCCCAATGCCTCCCTGGGCCGGGCCAACCCCCCCGCGGTGCTCGCCGCCGGGCCGGCCGGGGCCGCGCGGGGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGtt < 5:329744/137‑1 (MQ=255) tGCACCAATGACAACCTGGGCCGGCCCAACCCCCCCGCGGGTAGCGCCGCCTGGCCGGCCGGCGCCGGGGGGGGGGGGGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGa < 3:152965/137‑1 (MQ=255) agcaATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGTCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGAAGTTGGCCACCGCACCCCGCACCATCAGGTTTAaga > 4:285122/1‑137 (MQ=255) ccaATGCCAACCGGGGCCGGGCCAACCCCCCCCGGGTGATGGCCGCCGGGCCGGCGGGGGCCGGGGGGGGGGGTGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAagag < 6:382977/136‑1 (MQ=255) aaCCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACCGCCCCCCGCCCCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGgccg > 3:296735/1‑137 (MQ=255) aaCCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCGGGTTGAAGAGGCACGGGCTGAACTCACCGgccg > 3:96509/1‑137 (MQ=255) cccGCGGTGATCGCCGCCGGGCAGGCCGGCGCCGCGCGCTGGGGGGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCcgcgc < 5:203408/137‑1 (MQ=255) ggTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCGGGCCCCTGGTATTTGGCCACCGCCCCCCGCACCTTCAGGTTGAAGTGGCAATGGCTCAACTCCCCgcccccccgccccccc > 3:222315/1‑137 (MQ=255) gccgccTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCCCCCCGCCCCATCAGGTTGAAGCGGCACTGGCTGACCTCACCGGCCGCCCGCGCGCCCTgttgtt > 2:13209/1‑137 (MQ=255) gccgccTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTgttgttg > 3:166788/1‑136 (MQ=255) gccgccTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTtc > 6:13908/1‑137 (MQ=255) tGGCCGGCCGGCGCCGTGGGGGGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCAc < 4:153675/137‑1 (MQ=255) gccggCCGGCGCCGAGCGGGGGGGTG‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCgg < 5:124248/134‑1 (MQ=255) ccggCGGGGGCCGAGCGCGGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCgg < 3:368886/135‑1 (MQ=255) cAGGCCGGCGCCGCGCGCGGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCgg < 1:10563/137‑1 (MQ=255) cggCCGGCGCCGAGGGGGGGGGGGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGc < 6:331925/136‑1 (MQ=255) ggCGCCGAGCGCTGGGGTGTGGGGGGGGGGGACTCGCACCTGGTAGTTGGCCACAGCCCCCCGCACCAGCAGGTTGAAGAGGCACTGGCTGAAATCACCGGCCGCCCTCGCGCCCTGTGGTTAACAACCGCCtaaaa > 6:48866/1‑134 (MQ=255) cgcgGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCt < 1:232429/137‑1 (MQ=255) ggggtggggggCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGCCATCa < 1:261800/137‑1 (MQ=255) | AGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGCCATCA > ACB122/434245‑434512 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |