| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 1,063,304 | 0 | A | C | 54.2% | ‑3.1 / 12.6 | 27 | intergenic (+54/+90) | aroP_1/CJ019_01001 | Aromatic amino acid transport protein AroP/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (1/10); new base C (1/12); total (2/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.12e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCG‑TTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAG > ACB122/1063169‑1063432 | ctcGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCG‑TTAGGTTTTCGGCGCCTGTGAGATCGAg < 6:369428/137‑1 (MQ=255) gTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCG‑TTAGGTTTTCGGCGCCTGTGAGATCGAGcgcc < 3:343834/137‑1 (MQ=255) tGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTAGGCCG‑TTAGGTTTTCGGCGCCTGTGAGATCGAGCGCcgcgc < 5:128680/137‑1 (MQ=255) ccGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCG‑TTAGGTTTTCGGCGCCTGTGAGATAGAGCCCCGCccgggggggg > 6:371174/1‑135 (MQ=255) ggCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCG‑TTAGGTTTTCGGCGCCAGTTTGATAGCGCGCCCCCCGGGCGgggcg > 2:53140/1‑136 (MQ=255) ggggggggggggCTTGCACGCTGAACACGGC‑CGGCAGGCCACGGGCTTATCGGGTTTGTCCGTACGGGCTATGCCG‑TTAGGTTTCCGGCGCCTGGCCCACCCCGCGCCGCGCGGGCGGCGCTCTATCTCACAGGCGa < 4:82576/131‑1 (MQ=255) gtggCTTTCACGCTGACCACGGCGCGCCCGGCCAAGGGCGGAGCGGGTTTGTTCGTAAGGGGTATGCCG‑TTAGGTTTTCGGCGCCTGTGAGAGCGCGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGc < 8:278937/137‑1 (MQ=37) aagacgGCGCGCCAGGCCAAGGGCGGAGCGGGTTTGTTCGTACGGGGTAGGCCT‑TTAGGTTTTCGGCGCCTGTGAGAGCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTc < 2:8409/137‑1 (MQ=25) cccggCCAAGGGGGGAGCGGGTTTGTGCGAACGGGGAAGGCCT‑TTAGGTTTGCGGCGCCTGTCAGAGCGCGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTg < 4:157102/134‑1 (MQ=25) ggCTGATCGGGTTTGTTCTAACGGGGTAGCCCG‑TTCGGTTTTCGGCGCCTTGCAGACCGCGCGCCGGGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTccc < 8:208326/137‑1 (MQ=37) tGATCGGGTTTGTGCGTACGGGGTATGCCG‑TTAGGTTTTCGGCGCCTGTGAGATCGCGCCCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCtt < 4:88468/137‑1 (MQ=255) ttggggTTTGTTCGTAGGGGGTA‑GCCGTTTAGGTTTGCGGCGCGTGGGAGAGCCCGGCCGGCGGGGGGGGCGCGCCATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTcc < 3:164874/134‑1 (MQ=255) tcggggTTTGTGCGTACGGGGTATGCGG‑TTTGGTTTGCGGCGCCTGTCCGCTCCGGCGCGGCGCGGGCGGCGGTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTcc < 1:231490/134‑1 (MQ=255) cgcGGGTTTGTTCGTCAGGGGCAGCTCG‑TTTGGTTTGCGGCGCCTGTGCGAGCGCCCGCCGCGCGGGCGGCGCGCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTcc < 4:35913/135‑1 (MQ=255) agcGGGTTTGTTCGTACGGGGTATGCCG‑TAAGGTTTGCGGCGCCTGGGAGAGCCCGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTcc < 1:53140/135‑1 (MQ=38) ccG‑TTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCa < 6:146745/137‑1 (MQ=255) tttggTTTTCGGCCCCGGTGAGAGGGTGCGCCGCGGGGGCGGGGGTCTATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgc < 5:85887/134‑1 (MQ=255) tttggTTTGCGCCGCCTGTGAGATCGCGCCCCGCGCGGGCGGCGCCCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATACCCCTCAATGCTGCCTCCGCCAAgc < 7:15369/134‑1 (MQ=255) tgtttttCGGCGCCTGTCTCAGCGCGCGCCGCGCGGGCGGCGAGCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcac < 7:60610/134‑1 (MQ=255) gTTTTGGGCGCCTGGGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcacgc < 1:215503/137‑1 (MQ=255) ttttCGGCGCCTGGGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcacgca < 1:87303/137‑1 (MQ=255) ttttCCGCGCCTTTGAGAGCGGGCGCGGCGCGGGCGGCGGTCTATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCATTGCTGCCTCCTCCAAgcacgca < 5:80866/137‑1 (MQ=255) ggCGCCTGGGAGCGCGACCGCCGCGCGGGCGGCGCGCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtg < 4:133783/137‑1 (MQ=255) ggCGCCTGGGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtg < 5:371174/137‑1 (MQ=255) cgcCGGTGACATCGAGCGCCGGGCGGGCGGCGCGCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtggt < 1:98987/137‑1 (MQ=255) cggtgACATCCCGCGCCGCGCGGGCGGCGCGCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTa < 3:170133/135‑1 (MQ=255) tgAGATCGCGCGCGGCGCGGGCGGCGCGCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAg < 4:318942/137‑1 (MQ=255) | CTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCG‑TTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAG > ACB122/1063169‑1063432 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |