Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1221,348,4270CG30.4% 72.7 / 12.3 49M1I (ATG→ATC) †CJ019_01261hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (6/26);  new base G (14/0);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.62e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGTAAGGGACGACAGTGCCGTTACGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCT‑CCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCA‑CCGTTCCGTCCACTTGCTCGATAACCTCGCGGGGATGCGCAATGCATGCGCATCCGCCCGCGCGGGCGTACCGACTTCG  >  ACB122/1348297‑1348560
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tgtAAGGGACGACAGTGCCGTTACGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCAtgggt                                                                                                                                   <  1:136507/137‑1 (MQ=255)
tgtAAGGGACGACAGTGCCGTTACGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGGGGGGTTGCAtgggg                                                                                                                                   >  8:25412/1‑136 (MQ=255)
 gtAAGGGACGACAGTGCCGTTACGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCAtgggtg                                                                                                                                  <  7:291526/137‑1 (MQ=255)
         cgacAGTGCCGTTACGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGtct‑c                                                                                                                         <  7:187165/137‑1 (MQ=255)
         cgacAGTGCCGTTACGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGtct‑c                                                                                                                         <  3:227657/137‑1 (MQ=255)
         cgacAGTGCCGTTACGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGGTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGtct‑c                                                                                                                         <  5:143549/137‑1 (MQ=255)
             aGTGCCGTTACGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGGGGGTGTGGGTGGGGGGGTGtcggcaaa                                                                                                                      >  1:205779/1‑131 (MQ=255)
             aGTGCCGTTACGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGGGGGGTTGGATGGGGGGGTGTGT‑Cccca                                                                                                                     >  3:249739/1‑137 (MQ=255)
              gTGCCGTTACGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCT‑CCACAg                                                                                                                    <  5:131071/137‑1 (MQ=255)
              gTGCCGTTACGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCT‑CCACAg                                                                                                                    <  4:181202/137‑1 (MQ=255)
                 ccGTTACGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGGTTGAATAGGTGGGTGTGTGCCACAGtt                                                                                                                  >  2:314530/1‑137 (MQ=255)
                    ttACGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGGATGGGTTGGTGTAT‑CAACAGGTTGta                                                                                                              >  4:289241/1‑137 (MQ=255)
                    ttACGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCT‑CCCCAGtttatt                                                                                                              >  2:313573/1‑134 (MQ=255)
                         cTTGATCACGCGCAGTTGGACGGGTGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGGCGGGTTGCATTGGTGGGTGTCT‑CCCCAGTTTAGATAttt                                                                                                         >  6:342396/1‑136 (MQ=255)
                            taTCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCT‑CCACAGTTTCTATATTGTTc                                                                                                      <  3:289241/136‑1 (MQ=255)
                            gATGACGCGCAGTGGGCCGGGGGCGGTGGCTGCCAGATCCTGGTTGGAATGGGCGGCCTGCGGCACCTGGGCCTGCGTGTTCTCGCGAGTCGTGTCGGATTGCATGGGTGGGTGTCT‑CCACAGTTTCTATATTGTTc                                                                                                      <  8:70622/137‑1 (MQ=255)
                            gATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCT‑CCACAGTTTCTATATTGTTc                                                                                                      <  3:365792/137‑1 (MQ=255)
                            gATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCT‑CCACAGTTTCTATATTGTTc                                                                                                      <  1:240548/137‑1 (MQ=255)
                            gATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCT‑CCACAGTTTCTATATTGTTc                                                                                                      <  7:347681/137‑1 (MQ=255)
                            gATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGAGGGTTCTCGCGAGTAGTGTGTGTTTTCATTGGTTTGTGTGT‑CCACAGTTTATTTATttttt                                                                                                      >  5:273013/1‑136 (MQ=255)
                              tCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGGGGGGTTGGATGGGTGGGTGTGT‑GGCCAGTTTGGATATTTTTTAg                                                                                                    >  2:183490/1‑137 (MQ=255)
                               cACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGTTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCT‑CCACAGTTTCTATATTGTTAAgg                                                                                                   >  8:384772/1‑137 (MQ=255)
                               cACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTGGGTTTGTGTGGGTGGGGGGCT‑GGACACTTTGTATAATTTTTAgg                                                                                                   >  3:12521/1‑137 (MQ=255)
                               cACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGGGGGGTTTGATGGGGGGGGGTGT‑CCACACTTTGTATATTGTTAAgg                                                                                                   >  7:174307/1‑137 (MQ=255)
                                    gcAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGGGGGTTTGGATGGGTGGGTGTGT‑GCACAGATTTGTTATTTTTTATGCGccg                                                                                              >  3:331550/1‑136 (MQ=255)
                                     cAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGGTTGCATGGGTGGGTGTCT‑CCACAGTTTCGATATTGTTAAGGCGCCtt                                                                                             >  6:189360/1‑137 (MQ=255)
                                      aGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGGGGGGTTGGATGGGTGGGGGTGT‑GGCCAGTTTTTATTTTGTTAAGgcggcgtt                                                                                            >  1:316565/1‑132 (MQ=255)
                                           gCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGGCGGTTTGGATGGGTGGGTGTCT‑CCACAGTTTATATATTGTTAAGGCGCCTTATgggg                                                                                       >  6:207287/1‑136 (MQ=255)
                                                   cGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGGCGGGTTGGATGGGTGGGTGGGT‑CCACACGTTCTATATTTTTCATGGGCCGTTTTGGCGGCcc‑ccc                                                                              >  4:78856/1‑136 (MQ=255)
                                                   cGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGGCGGGTTGGATGGGGGGGTGGGT‑GCACAGGTTGTATATTGTTGAGGGGGCGTTTTGGCGCCCA‑ccc                                                                              >  4:231536/1‑136 (MQ=255)
                                                     gTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGGGGGTTTGGATGGGTGGGGGTGT‑GCACAGTTTGTATTTTTTTCAGGCGCCTTATTGGCGGCCC‑CCCtt                                                                            >  6:273420/1‑137 (MQ=255)
                                                         cAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGGGGGTTTGAATGGGTGGGTGTGT‑CCACAGTTTGTATATTTTTCAGGCGGCGTATTGGCGCCCC‑CCCTGCCgg                                                                        >  6:124426/1‑136 (MQ=255)
                                                           gCCAGATCCTGGTTGGAATCGGCGTCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCT‑CCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCA‑CCGTTCCGTcc                                                                      <  7:295494/137‑1 (MQ=255)
                                                                  ccTGGTTGTAATCGGCGGCGGGGGGCCCGTGGGCGTGGTGGTTCTCGCTAGTGGTGTGGGTTGGCATGGGTGGGTGTCT‑CCACAGTTTCTATATGTTTCAGGCGCCTTATGGGCGCCCA‑CCGTTCCGTCCACTTGCt                                                               <  8:255352/137‑1 (MQ=255)
                                                                   cTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTGGGTTTTGATGGGTGGGGGTGT‑CCACAGTTTCTATTTTGTTTAGGGGGCGTATGGGCGCCCC‑CCGGTGCGGGCACTTGcgg                                                              >  3:87057/1‑135 (MQ=255)
                                                                   cTGGTGGGACTCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCT‑CCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCA‑CCGTTCCGTCCACTTGCTc                                                              <  6:104248/137‑1 (MQ=255)
                                                                     ggTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCT‑CCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCA‑CCGTTCCGTCCACTTGCTCGa                                                            <  5:180584/137‑1 (MQ=255)
                                                                          taaTCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGGGTCT‑CCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCA‑CCGTTCCGTCCACTTGCTCGATAAcc                                                       <  3:67821/136‑1 (MQ=255)
                                                                            aTCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCT‑CCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCA‑CCGTTCCGTCCACTTGCTCGATAACCTc                                                     <  5:280641/137‑1 (MQ=255)
                                                                            aTCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCT‑CCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCA‑CCGTTCCGTCCACTTGCTCGATAACCTc                                                     <  7:59759/137‑1 (MQ=255)
                                                                              cggcggCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGGGGGGTTGGATGGGGGGGGGTGT‑CCACCCTTTATATATTTTTAAGGCGCCTTTTGGGCGCCCACCCGTTCGGACC‑CTTGCTCGATAAccaacc                                                   >  4:143585/1‑133 (MQ=255)
                                                                                    cgtGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCT‑CCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCA‑CCGTTCCGTCCACTTGCTCGATAACCTCGCGGGGAt                                             <  4:58396/135‑1 (MQ=255)
                                                                                    ccggCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGTTTTGCATGGGTGGGTGTCT‑CCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCA‑CCGTTCCGTCCACTTGCTCGATAACCTCGCGGGGAt                                             <  8:244079/134‑1 (MQ=255)
                                                                                         ggCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTGGGTTTGGTTGGGGGGGTGTGT‑CCCCAGTGTGTATTTTGTTGAGGCGCGTGATGGGGGGCCC‑CCGGTCCGGCCCCTTTCGCGATAACCTCGCGGGGGTGGGCa                                        >  4:232479/1‑137 (MQ=255)
                                                                                                           ttCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCT‑CCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCA‑CCGTTCCGTCCACTTGCTCGATAACCTCGCGGGGATGCGCAATGCATGCGCATccgccc                      <  5:189360/137‑1 (MQ=255)
                                                                                                           ttCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCT‑CCACAGTTTCTATATTGTGCAGGCGCCTTATGGGCGCCCA‑CCGTTCCGTCCACTTGCTCGATAACCTCGCGGGGATGCGCAATGCATGCGCATccgccc                      <  7:25412/137‑1 (MQ=255)
                                                                                                                   aGTCGTGTCGGTTTGCATGGGTGGGTGTCT‑CCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCA‑CCGTTCCGTCCACTTGCTCGATAACCTCGCGGGGATGCGCAATGCATGCGCATCCGCCCGCGCGGGc              <  8:174307/137‑1 (MQ=255)
                                                                                                                          gcGGTTTGCATGGGTGGGTGGCT‑CCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCA‑CCGTTCCGTCCACTTGCTCGATAACCTCGCGGGGATGCGCAATGCATGCGCATCCGCCCGCGCGGGCGTACTGa       <  1:313573/136‑1 (MQ=255)
                                                                                                                               ttGCATGGGTGGGGGTCT‑CCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCA‑CCGTTCCGTCCACTTGCTCGATAACCTCGCGGGGATGCGCAATGCATGCGCATCCGCCCGCGCGGGCGTACCGACTTCg  <  3:231536/137‑1 (MQ=255)
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TGTAAGGGACGACAGTGCCGTTACGCTTGATCACGCGCAGTTGGCCGGGGGCGGTGGCAGCCAGATCCTGGTTGGAATCGGCGGCCTGCGGCACCTGGGCCTGCGGGTTCTCGCGAGTCGTGTCGGTTTGCATGGGTGGGTGTCT‑CCACAGTTTCTATATTGTTCAGGCGCCTTATGGGCGCCCA‑CCGTTCCGTCCACTTGCTCGATAACCTCGCGGGGATGCGCAATGCATGCGCATCCGCCCGCGCGGGCGTACCGACTTCG  >  ACB122/1348297‑1348560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑