Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1222,386,9650GT24.3% 64.4 / 11.0 37intergenic (+34/+72)CJ019_02252/dacC_1hypothetical protein/D‑alanyl‑D‑alanine carboxypeptidase DacC
Reads supporting (aligned to +/- strand):  ref base G (7/21);  new base T (9/0);  total (16/21)
Fisher's exact test for biased strand distribution p-value = 9.20e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGGTCGGGCGAAGGCGCCGATGCCTTGCTGTTGCGCGCCGACGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCTTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCAACACCTGATC  >  ACB122/2386830‑2387099
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cGGTCGGGCGAAGGCGCCGATGCCTTGCTGTTGCGCGCCGACGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCTTTgg                                                                                                                                       >  8:141657/1‑136 (MQ=255)
       gCGAAGGCGCCTATGCCTTGCGGTTGCGCGCCGACGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCTTTGCGGCCCtt                                                                                                                                <  1:244371/137‑1 (MQ=255)
       gCGAAGGCGCCGATGCCTTGCTGTTGCGCGCCGACGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCTTTGCGGCCCtt                                                                                                                                <  3:297613/137‑1 (MQ=255)
                ccGATGCCTTGCTGTTGCGCGCCGACGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCTTTGCGGGCCTTTGGGGACgg                                                                                                                       >  8:72985/1‑136 (MQ=255)
                      ccTTGCTGTTGCGCGCCGACGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCTTTGCGGCCCTTTCGGGACGCAAAGcc                                                                                                                 <  6:308696/137‑1 (MQ=255)
                            tGTTGCGCGCCGACGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCTTTGCGGCCCTTTAGGGACGCAAAGCCGCTccg                                                                                                           >  1:290021/1‑136 (MQ=255)
                                gcgcgcCGACGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGGGGGGGCCGCTTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCAt                                                                                                       <  8:353207/137‑1 (MQ=255)
                                        aCGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCTTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACcgcg                                                                                               <  8:94848/137‑1 (MQ=255)
                                          gCCGGCTTATATGCGGCCAAACCGGCCGGGCGGGACCGGGGGGAGGGGGAGCCGGTATAAACCCGTGCGCGCGGTCGGCGTGGGGGCCGCTTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCa                                                                                             <  8:47761/137‑1 (MQ=255)
                                          gCCGGCGTATATGCGGCCAAACCGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCTTTGCGGCCCGTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCa                                                                                             <  7:196192/137‑1 (MQ=255)
                                               ggTATATGCGGCCAAACCGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCTTTGCGGCCCGTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCgg                                                                                        <  2:122764/136‑1 (MQ=255)
                                                ttattTGCGGCCAAACAGGCGGGGCGGGACCGGGTGGTGGGGGTGCCGTTATAAACCCGTGCGGGGGGTCGGCGTGGGGGCCGCTTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCggg                                                                                       <  4:310476/136‑1 (MQ=38)
                                                ttataTGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCTTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCggg                                                                                       <  5:279122/136‑1 (MQ=255)
                                                      gctgCCAACAAGGCGGGGCGGGACCGGGTGTTGGGGGAGCCTGTATAATACCGTGCGGGCGGTCGGCGTGGGGGCCGCTTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCggg                                                                                 <  3:50194/134‑1 (MQ=37)
                                                            aaaCAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCTTTTCGGCCCGTTGGGGGCCCCAACCCGCGCAGGGAGAGCACGCGCCACCGGGCCCCGGggtgtg                                                                           >  3:130496/1‑137 (MQ=255)
                                                            aaaCAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCTTTTCGGCCCGTTCGGGGCGCAAAGCCGGGCCGGCATCGACCGCGCAAGCGGGCCCGGGtgtgtg                                                                           >  7:203623/1‑137 (MQ=255)
                                                                aGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCTTTTCGGCCCGTTCGGGACGCAAAGCCGCTCCGGGATCGACCGCGCCAGCCGGCCCGGGTGTGTGGccg                                                                       >  4:343633/1‑135 (MQ=255)
                                                                aGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCTTTGCGGCCCGTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAg                                                                       >  4:104178/1‑137 (MQ=255)
                                                                         gggACCGGGTGGAGGGGGAGCCGGTATAAAACCGGGCGCGCGGTCGGCGTGGGGGCCGCTTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCa                                                              <  2:234221/137‑1 (MQ=255)
                                                                               gggTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCTTTGCGGCCCTTTCGGGCCGCAAAGCCGCTCCTGCATCGACCCCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGc                                                        >  4:296579/1‑137 (MQ=255)
                                                                                        gggAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCTTTTCGGCCCTTTAGGGACGCAAACCCCCACCAGCAACCACCCCGCCACCGCGCCCGGGGGGGTGGCAGGTGGCGTGAGTTTGCCGCTGTACt                                               >  5:274521/1‑137 (MQ=255)
                                                                                           aGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCTTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTgcc                                            <  3:104178/137‑1 (MQ=255)
                                                                                                  ataAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCTTTTCGGCCCGTTGGGGCCGGAAAGCCGGTCCGGCATCGACCCCGCCAGCCGGCCCGGGTGGGTGGCAGGTGGCCTGACTTGGGCGCTGTACTgccagccacc                                     >  5:106577/1‑135 (MQ=255)
                                                                                                   aaaaaCCGTGCGCGCGGTCGGCGGGGGGGCCGCTTTGCGGCCCTGTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCt                                    <  1:225792/136‑1 (MQ=255)
                                                                                                        ccGTGCGCGCGGGCGTCGGGGGGGCCGCTTTGCGGCCCTTTCGGTCAGCAAAGCCGCTCCTGCATCGACGGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGc                               <  8:105321/137‑1 (MQ=255)
                                                                                                             ggggcgGTCGGCGTGGGGGCCGCTTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCg                          <  7:141657/134‑1 (MQ=255)
                                                                                                             ggcgcgGTCGGCTTGGGGGCCGCTTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCg                          <  6:31177/136‑1 (MQ=255)
                                                                                                             cgcgggGTCGGCGTGGGGGCCGCTTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCg                          <  6:26385/137‑1 (MQ=255)
                                                                                                              gcgcgGTCGGCGTGGGGGCCGCTTTTCGGCCCGTTCGGGCCGCCAAGCCGCTCCTGCATCGACCGCGCCAGCGGGCCCGGGTGGGTGGCAGGTGGGGTGAGTTTGCCGCTGTACTGCCAGCCAGGTTCGGCTGGCgg                         >  8:245011/1‑136 (MQ=255)
                                                                                                              gcgcgGTCGGCGTGGGGGCCGCTTTTCGGCCCGTTAGGGCCGCAAAGCCGCTCCGGCATAGCCCCCGCAAGCGGGCCCGGGTGGGTGGGAGGGGGCGTCAGGTGGCCGCTGTACTGCCCGCCAGCTTCGGCTggggc                         >  5:136871/1‑134 (MQ=255)
                                                                                                              gcgcgGTCGGCGTGGGGGCCGCTTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCgg                         >  7:376619/1‑136 (MQ=255)
                                                                                                              gcgcgGTCGGCGTGGGGGCCGCTTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGa                         >  8:324961/1‑137 (MQ=255)
                                                                                                              gcgcgGTCGGCGTGGGGGCCGCGTTTGGGGCCGTTGGGGGCGCCAAGCCGCGCCGGGCTCGCACGCCCAACCGGGCCCCGGGGTGTGGGCGGTGGGGGGCGTTGGCCCCGGTTCTGCCCCCCCGCGTGggcgggcgg                         >  6:2725/1‑136 (MQ=255)
                                                                                                              acgcgGTCGGCGTGGGGGCCGCGTTTCGGCCCATTGGGGGCGCCAAGCCGCGCCGGCATCGACCGCGCCAGCGGGCCCGGGGGTGTGGGAGGTGGCGTCCGGTGGGCCCTGTACAGCCAGCCCGCGTCGGCTGGCgc                         >  2:303508/2‑136 (MQ=255)
                                                                                                                        ggTGGGGGCCGCTTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCaa               <  4:36813/136‑1 (MQ=255)
                                                                                                                             gggCCGCTTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGGGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCAac          <  6:342472/137‑1 (MQ=255)
                                                                                                                                  gCTTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCAACACCTg     <  7:202447/137‑1 (MQ=255)
                                                                                                                                     ttGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATAGACCGCGCAAGCGGGCCCGGGGGTGTGGCAGGTGGCGTCAGTTGGCCGCTTTACTGCCAGCCAGCTTCGGCTGGCGCTACAGGGGCAGCAACCCCTgcta  >  6:307141/1‑134 (MQ=255)
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CGGTCGGGCGAAGGCGCCGATGCCTTGCTGTTGCGCGCCGACGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCTTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCGCAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCAACACCTGATC  >  ACB122/2386830‑2387099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑