Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,138,3670TG32.1% 72.1 / 17.7 56F240V (TTT→GTT) rbsC_2Ribose import permease protein RbsC
Reads supporting (aligned to +/- strand):  ref base T (16/22);  new base G (17/1);  total (33/23)
Fisher's exact test for biased strand distribution p-value = 1.45e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGTACACGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTGG  >  ACB122/3138233‑3138499
                                                                                                                                      |                                                                                                                                    
aGTACACGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGttt                                                                                                                                    >  7:251412/1‑137 (MQ=255)
    cacGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACTTGTTCGCGAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGtt                                                                                                                                >  3:11816/1‑137 (MQ=255)
     acGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGttt                                                                                                                               >  1:119028/1‑136 (MQ=255)
         cATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTcggcg                                                                                                                           <  6:252595/137‑1 (MQ=255)
         cATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTcggcg                                                                                                                           <  6:93351/137‑1 (MQ=255)
          aTTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTcggcgg                                                                                                                          >  8:342439/1‑137 (MQ=255)
          aTTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTgcgcgg                                                                                                                          >  8:24910/1‑137 (MQ=255)
          aTTCGGACTGCGTCTGTAGGCCATCGGAGAATACCCGTAGGCGGCGAATGCCGCTGGCCTGTCGGTGCGGGGCTACGTTTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTcggcgg                                                                                                                          <  6:237190/137‑1 (MQ=255)
               gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTgg                                                                                                                     >  7:268935/1‑137 (MQ=255)
               gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTgg                                                                                                                     >  3:331631/1‑137 (MQ=255)
               gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGGGGCCAgg                                                                                                                     >  2:209206/1‑137 (MQ=255)
                  tGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTc                                                                                                                  <  2:11777/137‑1 (MQ=255)
                    cGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAg                                                                                                                <  4:231922/137‑1 (MQ=255)
                      tCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCg                                                                                                              >  8:20629/1‑137 (MQ=255)
                      tCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGCGGCCGGGTTCAGCg                                                                                                              >  2:94295/1‑137 (MQ=255)
                      tCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGCGGCGTGGGTCAGCg                                                                                                              >  1:102745/1‑137 (MQ=255)
                      tCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTAGGCGGCCGGGTTCAGCg                                                                                                              >  4:96910/1‑137 (MQ=255)
                             gCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGcac                                                                                                       <  2:313815/137‑1 (MQ=255)
                                  cGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCg                                                                                                  >  8:381664/1‑137 (MQ=255)
                                  cGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCg                                                                                                  <  4:331631/137‑1 (MQ=255)
                                  cGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTTTTGGGCGGCCTGGTTCAGCCGCCGCcccaccc                                                                                                  >  3:31801/1‑136 (MQ=255)
                                    gagaATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTTTTCGGCGGGCTGGTTGAGCGGCAGCACCCCCggg                                                                                                >  7:360169/1‑136 (MQ=255)
                                    gagaATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCCGCCGCACCACCGGt                                                                                                >  7:248079/1‑137 (MQ=255)
                                    gagaATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTAGGCGGCCTGGTTCAGCGGCCGCACCCCCGGt                                                                                                >  4:153921/1‑137 (MQ=255)
                                    gagaATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTGTTGGTGGGCGGGCTGGTTCAGCGGCAGCaccccccgg                                                                                                >  4:193923/1‑134 (MQ=255)
                                    gagaATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGCGGCCTGGTTCAGCGGCAGCaccacccgg                                                                                                >  2:80981/1‑134 (MQ=255)
                                            ccGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGGGGGCAGGGTCAGCGGCAGCCCCACCCGTTCCGGCgc                                                                                        >  6:213900/1‑136 (MQ=255)
                                            ccGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCga                                                                                        >  6:24707/1‑137 (MQ=255)
                                            ccGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCGGATCCGCGGGCTGTGCGGCAGCCTGGCCGCGGGTTTTTAGTCCGCCGCGGTCAGCGGCCGCCCCCCCCGGGCCGGCgg                                                                                        >  6:213898/1‑136 (MQ=255)
                                                aGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGAtg                                                                                    <  7:313147/137‑1 (MQ=255)
                                                aGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGAtg                                                                                    <  7:24910/137‑1 (MQ=255)
                                                aGGCGGCTAATGCCGCTGGCCTGTCGGTGCGGGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCATCCTGGCCGCGTTTTGTTCTGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGAtg                                                                                    <  2:25396/137‑1 (MQ=255)
                                                  gcggcTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGACTGGCCGCGGTTTGTTGGGCGGCCGGGTTCAACGGCCGCACCACCGGGTCCGGCGAGAtgct                                                                                  >  4:105197/1‑137 (MQ=255)
                                                      cTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTTTTAGGCGGCCGGGGTCAACGGCCGCACCCCCCGTTCCCGCGAGATGcagctg                                                                              >  6:123082/1‑137 (MQ=255)
                                                       tAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGGTGGGGGGCCGGGTTGAGCGGCCGCCCCCCCCGGTGCGGCGAGATGctgctgg                                                                             >  1:58753/1‑136 (MQ=255)
                                                                  gcatGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTGTGTTCGGCGGGCTGGGTGAGCGGCAGCACCCCCGGGTCCGGCGAGATGCTGCTGTACGTGGTGGcc                                                                  >  8:379565/4‑137 (MQ=255)
                                                                  gCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGCGGGCTGGTTGAGCGGCAGCACCACCCGTTGAGGCGAGATGGTGGTGTGCGtggtgggg                                                                  >  2:55789/1‑135 (MQ=255)
                                                                  gCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGCGGCCGGGTTGAGCGGCAGCACCCCCGGGTGCGGCGCGATGGTGGGGTGCGgggtgggg                                                                  >  7:144727/1‑135 (MQ=255)
                                                                  gCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGGTTTTTTGGGGGCCGGGGTGAGCGGCCGCCCCCCCCGGGGCGGCGCGCTGGTGGGGTGCGtggtgggg                                                                  >  1:358690/1‑135 (MQ=255)
                                                                    cTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCAt                                                                <  6:312405/137‑1 (MQ=255)
                                                                    cTGTCGGTGCGGGGCTTCGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCAt                                                                <  8:312893/137‑1 (MQ=255)
                                                                              gTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCtt                                                      <  1:35768/137‑1 (MQ=255)
                                                                                ggCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTgg                                                    <  5:24707/137‑1 (MQ=255)
                                                                                    aCGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTg                                                <  7:342439/137‑1 (MQ=255)
                                                                                         ttCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGAtctt                                           <  8:117808/137‑1 (MQ=255)
                                                                                            gCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTGGGCGGGCGGGTTCAGCGGCAGCACCCCCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCCTCGCTTTTGGTGGGGTGAtcttata                                        >  8:159754/1‑134 (MQ=255)
                                                                                            gCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTtctc                                        <  7:288559/137‑1 (MQ=255)
                                                                                            gCCAGCTACCTGATCAGCGGGCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTtctc                                        <  5:217801/137‑1 (MQ=255)
                                                                                               aGCTACCTGTTCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGCGGCCGGGTTAAGCGGCAGCCCCCCCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCGTTTATTGGGGTGATGTTCTCCCa                                     >  4:180980/1‑137 (MQ=255)
                                                                                                    ccTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTTTTGGGCGGGCGGGTTGAGCGGCCGCCCCCCCCGGTGCGGCGAGATGCTGCTGTGCGGGGTGGGGCTCGCGTTTGTTGGGGTGGTCTTATACCAGCGtg                                >  3:70542/1‑136 (MQ=255)
                                                                                                           aGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTAGGCGGCCTGGTTAAGCGGCAGCACCACCGGGTACGGCGAGATGCTTCTTTCCGTGGTGGCCATCGCTTTTCTTTGGGTGATCTTCTACCACCCTCTGCAGcc                         >  5:127046/1‑137 (MQ=255)
                                                                                                              ggTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAg                      <  2:358690/137‑1 (MQ=255)
                                                                                                                tCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTACGGCGCGATGCTGCTGTCCGTGGTGGCCATCGCGTTTGTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGACCa                    >  7:129438/1‑137 (MQ=255)
                                                                                                                    tgCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATcgg                <  7:381664/137‑1 (MQ=255)
                                                                                                                                  cgcgTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTgg  <  5:213900/137‑1 (MQ=255)
                                                                                                                                  cgcgTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTgg  <  3:96910/137‑1 (MQ=255)
                                                                                                                                  cgcgGTGTGGTCGGCGGCCGGGTTCAGCGGCAGCACCCCCGGGTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGCTCTTCTCCCCGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTgg  <  5:213898/137‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                    
AGTACACGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTGG  >  ACB122/3138233‑3138499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑