Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,440,3630AC53.8% 7.9 / 27.6 39A24A (GCA→GCC) CJ019_03266hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (12/6);  new base C (21/0);  total (33/6)
Fisher's exact test for biased strand distribution p-value = 5.69e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.89e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGAT  >  ACB122/3440236‑3440495
                                                                                                                               |                                                                                                                                    
aGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCTGGgtg                                                                                                                             >  3:219482/1‑137 (MQ=255)
aGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCCGCCGGGgtg                                                                                                                             >  2:63449/1‑137 (MQ=255)
 gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGgtgt                                                                                                                            >  5:357844/1‑137 (MQ=255)
 gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCCCAGGCGGCGCCCGGGCCCCCGGGgtgt                                                                                                                            >  5:19085/1‑137 (MQ=255)
        tCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTCCTGCTGGCCCCCAGCGCGGGCGGGGCCCCGGCACCCCCGGTGTgggcggc                                                                                                                     >  3:238650/1‑132 (MQ=255)
               gaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCCGCCAGGGGGGGGCAGGcccccccc                                                                                                              >  1:265997/1‑131 (MQ=255)
                aTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGCCAACGcca                                                                                                             >  5:21393/1‑136 (MQ=255)
                    cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCGGGGGGTGGCCGGCCACCGCCTATaa                                                                                                         >  5:348494/1‑137 (MQ=255)
                    cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCCCCCTGGCGGCGCCCGGGCAGCCAGGGTGTGGGGGGCCACCCCCTATaa                                                                                                         >  7:145154/1‑137 (MQ=255)
                     aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCTGGGTGTGGCAGGCCAACCCCTATAAg                                                                                                        >  7:223403/1‑137 (MQ=255)
                     aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCGGGGTGTGGCAGGCCCACGCCTATAAg                                                                                                        >  5:98744/1‑137 (MQ=255)
                     aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCGCGCGGGCGGCGGCCGGGCAGCGAGGGTGTGTGAGGCCAAAGCCTATaaa                                                                                                        >  1:234339/1‑136 (MQ=255)
                       ggCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCGGGCCGCCTGGGTGTGGCACGCCCACCCCGATAATgc                                                                                                      >  2:39233/1‑137 (MQ=255)
                            tctACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCCCGCGTGCGGCGGCCCGGCCCCCTGGGTGGGGCCGGCCACACCCCAAAAGGCGCAGc                                                                                                 >  2:367134/1‑137 (MQ=255)
                              tACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCCGCCTGGGTGTGGCCGGCCCACGCCGATAAGGCGCAGCTc                                                                                               >  5:327693/1‑137 (MQ=255)
                               aCAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGGGGCAGGCCAACCCCTATAATGCGCAGCTCg                                                                                              >  5:6727/1‑137 (MQ=255)
                                      agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCCGGCCGCCTGGGTGTGGCAGGCCAACGCCGATAAGGAGCAGCTCGCGGATCa                                                                                       >  3:149506/1‑137 (MQ=255)
                                      agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGCGGCGCCCGGGCACCCAGGGTGTGGCAGGCCACCCCCGCTAAGGAGCACCGCGCTGATCa                                                                                       >  6:108749/1‑137 (MQ=255)
                                         gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCCGGCCCCCGGGGGGTGGGAGGCCCACGCCGATAAGGCGCCGCGCGCGGATAAGGc                                                                                    >  4:119541/1‑137 (MQ=255)
                                         gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCAGGCGGCGCCCGGGCCGCCGGGGGGGGGCCGGGCCACGCCTCAAAGGCGCAGCGCCCGGATCATGc                                                                                    >  3:102465/1‑137 (MQ=255)
                                         gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCACCGCTGGCGGCGCCCCGGCACCCGGGGTGTGGCAGGCCCCCGCCGATAAAGCCCACCTCGCGGCTCCGGc                                                                                    >  8:319813/1‑137 (MQ=255)
                                         gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCACCGCTCGCGGCGGCCGGGCCGCCGGGGGGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCACGc                                                                                    >  3:180849/1‑137 (MQ=255)
                                           tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCCGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCGGATCAGGCCg                                                                                  >  6:363657/1‑137 (MQ=255)
                                                              gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCCCCCGGGCGGCGCCCGGGCCGCCGGGGGGTGGGCGGCCCACCCCGATAAGGCGCCGCTCGCGGAGAACGCCGCTGGCTTAGGCAACCAGtt                                                               >  8:136026/1‑137 (MQ=255)
                                                              gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGGCGCCGGGGGGTGGCCGGCCAACGCCTATAATGCGCAGCTCGCTGATCAGGCCGATGACTTAGGGCAGCAGtt                                                               >  2:311756/1‑137 (MQ=255)
                                                              gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGGAGCCGGGGGGTGGGAGGCCCACCCCGATAAGGCGCCGCTCGCGGATGACGCCGAGGACTTAGGCAAGCAggt                                                               >  2:116799/1‑135 (MQ=255)
                                                              gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCGGGGGGTGGCCGGCCCACGCCGATTAGGGGCGGCTGGCTGATAAGGCCGCTGACGTCGGCAAGCAgat                                                               >  7:245966/1‑135 (MQ=255)
                                                              gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCGCGCGGGCGGCGCCCGGGCCGCCAGGGTGTGGGCGGGCCACGCCTATAATGCGCAGCTCGCGGGTCAGGCCGCTGTCTTCGGCAAGAAGtt                                                               >  2:168009/1‑137 (MQ=255)
                                                                                          ggctggtgcgGGCCCCCCGGGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTg                                   <  5:105466/136‑1 (MQ=255)
                                                                                          cgcgGCTGGTGGCCGCCAGCGGGGCCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTg                                   <  5:344951/137‑1 (MQ=255)
                                                                                          cgcTGCTGCTGGGCGCCCCCGCGGGCGGCGCCCCGGCCGCCGGGGTGTGGGCGGCCCACCCCGATAAGGCGGCGCGCGCGGTTCAGGCCGATGACGTCGGGCAGCAGTTGGCGGAGAAGGATCGCGCGCCCGGGCTg                                   >  4:26214/1‑137 (MQ=255)
                                                                                                     gccgccGGCGGGGGGGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGaa                        <  4:238650/137‑1 (MQ=255)
                                                                                                     gccgccGGCGGCGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGaa                        <  7:136026/137‑1 (MQ=255)
                                                                                                            gcgcTGGCGGCGCCCGGGCCGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGCTCAGGCCGATGACGTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCCCGGTCTGGAGCGGGAGAAGGCTgcc                 >  8:64733/1‑136 (MQ=255)
                                                                                                            gcgcTGGCGGCGCCCGGGCCGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCACGCTGATCAGGCCGATGAATTCGGCAAGCAGTTTGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTgca                 >  8:294793/1‑137 (MQ=255)
                                                                                                                 ggcggcGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTgcagcagc            <  8:335228/137‑1 (MQ=255)
                                                                                                                       gcCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCt      >  8:31144/1‑137 (MQ=255)
                                                                                                                        cccGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTc     <  6:21393/137‑1 (MQ=255)
                                                                                                                           gggCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGAt  >  3:91611/1‑137 (MQ=255)
                                                                                                                               |                                                                                                                                    
AGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGAT  >  ACB122/3440236‑3440495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑