Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,384,4080TG35.5% 35.0 / 11.1 31intergenic (+529/‑329)CJ019_05064/CJ019_05067putative protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (2/18);  new base G (11/0);  total (13/18)
Fisher's exact test for biased strand distribution p-value = 9.21e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGG  >  ACB122/5384280‑5384544
                                                                                                                                |                                                                                                                                         
tCGGGCGGCTGCGGATTAACTCAGTAGACACGGCTAAGCATGTAGACCCGACAGCGTAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTtgt                                                                                                                                   <  2:277057/137‑1 (MQ=255)
gcGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTtgt                                                                                                                                   <  7:365694/136‑1 (MQ=255)
    gcggcTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCGCGGGCGTGTTTTTTtgtgggg                                                                                                                               >  8:49050/1‑134 (MQ=255)
          gCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCGTTTTTTGTGGCG‑TGAAtt                                                                                                                        >  6:104845/1‑137 (MQ=255)
                           acacGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCGTTTTTTTTGGCT‑GGAATTTTGGCGCTGCGGGGCgt                                                                                                       >  5:356626/1‑136 (MQ=255)
                               cgCTAAGCATGTAGTACCGACAGCGTAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGaaaaa                                                                                                   <  3:50543/136‑1 (MQ=255)
                                  tAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGGGGGGTTTTGTGTCG‑GGTATTTGGGGGCTGGGGGGCGAAAAATCg                                                                                                >  7:139819/1‑137 (MQ=255)
                                           agtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAcc                                                                                       <  1:116976/137‑1 (MQ=255)
                                           agtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAcc                                                                                       <  6:336846/137‑1 (MQ=255)
                                           agtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAcc                                                                                       <  4:164521/137‑1 (MQ=255)
                                             taCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGGGGGGTTTTTTGCATGGGAATTTGGGC‑CGGCCGGGGGAAAAATACGATTTAAcccc                                                                                     >  5:115372/1‑137 (MQ=255)
                                              aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGTGGCT‑GGTATTTGGGGGGGGCGGGGGGAAAAATAGGATTTAAaccca                                                                                    >  5:393165/1‑136 (MQ=255)
                                              aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCGTTTTTTGTTTCA‑GGAATTTTGTGGGTGCGGGGCGAAAAAATGGTTTTAAccaca                                                                                    >  1:39173/1‑134 (MQ=255)
                                                   cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTGGCT‑GGAATTTGGGCGGGGCGGGTCGAAAAATAGGATTTTACCCCTTTGtt                                                                               >  6:391031/1‑137 (MQ=255)
                                                   cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTGGCG‑GGGATTTGGGGGCTGCGGGGGGAAAAATAGGATTTTAACCCtttggt                                                                               >  6:226058/1‑134 (MQ=255)
                                                     gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGTGTTTTTTTGGCT‑GGAATTTGGGGGGTGCGGGTCGTAAAATCGGCTTTTACCCCTTTCTTGt                                                                             >  2:275487/1‑137 (MQ=255)
                                                     gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGGTTTTTTTGGCG‑GGGATTTTGGGGGGGGGGGTGGAAAAATCGGTTTTTAACCCttttatgt                                                                             >  3:257435/1‑132 (MQ=255)
                                                         aGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAggg                                                                         <  1:302511/137‑1 (MQ=255)
                                                                     cGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAg                                                             <  2:279511/137‑1 (MQ=255)
                                                                        gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGGTTTTTGTTGGT‑GGGTTTTTGGGGCTGGGGGGGGGAAAAAAGGATTTTAACCCGTTGTTGTTTGGGGTTTGGGAAAGATa                                                          >  5:382820/1‑137 (MQ=255)
                                                                                                   aTCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGt                               <  4:196650/137‑1 (MQ=255)
                                                                                                      cAAAGAAGAAGACGCCCTCGGGCGTCGTTGTTTGTTGCT‑GGGATTTTGGCGCAGGGGGTCGTAAAAAAGGATTTTACACAAATATTGTTGGGCGGGTCCCAAAGATAATGGCAACGCCTGGTTTCAGTGGGGGTGAt                            >  2:109037/1‑137 (MQ=255)
                                                                                                          gaagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                        <  4:203599/137‑1 (MQ=255)
                                                                                                          gaagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                        <  3:123045/137‑1 (MQ=255)
                                                                                                          caagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                        <  3:370017/136‑1 (MQ=255)
                                                                                                               agaCGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGGGTTTCCCAAAGATACTGCCTGCGCCTGATTTCCGTTGCGGTCATTTTTTCAgg                   <  5:170545/137‑1 (MQ=255)
                                                                                                                 aCGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGACTTTGGGGGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGt                 <  8:178997/137‑1 (MQ=255)
                                                                                                                  cGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGtt                <  4:252708/137‑1 (MQ=255)
                                                                                                                      cTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAg            <  7:75363/137‑1 (MQ=255)
                                                                                                                         gggCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtct         <  6:71377/137‑1 (MQ=255)
                                                                                                                              tCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGAGTTTCCCATAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTg    <  8:275124/137‑1 (MQ=255)
                                                                                                                                tttttttGTGCCT‑GGACTTTGGTCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTggg  <  6:384958/137‑1 (MQ=255)
                                                                                                                                |                                                                                                                                         
TCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCT‑GGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGG  >  ACB122/5384280‑5384544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑