Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,688,6370AC43.8% 24.4 / 12.3 50S236R (AGC→CGC) pyrC'Dihydroorotase‑like protein
Reads supporting (aligned to +/- strand):  ref base A (8/19);  new base C (6/15);  total (14/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTG‑CTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGC  >  ACB122/5688513‑5688769
                                                                                                                             |                                                                                                                                     
cggTGCCATGGCCAGCTTCCTCGGCCTGCGGGGCATCCCGGAGAGCGCCGAAACCGGGGCCCGGGCGCGCAACCTGCGG‑CTGGGGTAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCCCCAGCGCCCGTGgcg                                                                                                                           <  8:236703/136‑1 (MQ=255)
aGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTG‑CTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGgcg                                                                                                                           >  5:287333/1‑137 (MQ=255)
     ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTG‑CTGGTGGAACAGAGCGGCGGGCGGGCGCCCTTACGCCAGCTGACCCCCGCCCGAGgcg‑ccccg                                                                                                                     >  3:17209/1‑133 (MQ=255)
           ccAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTG‑CTGGTGGAACAGAGCGGCGTGGGTGCGCACTTCAGCCAGCTCACCCGCGCCCGTGGCG‑CGCGGCTGATc                                                                                                               >  4:273417/1‑137 (MQ=255)
           ccAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTG‑CTGGTGGAACAGAGCGGCGTGCGTGCGCACGTCAGCCCCCTCACCCGCGCCCGTGGCG‑CGCGGCGGAta                                                                                                               >  1:41949/1‑136 (MQ=255)
           ccAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTG‑CTGGTGGAACAGAGCGGCGGGGGGGGGCCCTTCAGCCCGCACACCAGCGCCCCTGGCG‑CGCGGCGGAta                                                                                                               >  3:342426/1‑136 (MQ=255)
           ccAGCGTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCTAAACCGTGGCCCTGGCGCGCAACCTGCGG‑CTGGTGTACCAGAGCGGCGTGCGTGCGCACTTCAGCCAGAACCCCAGCGCCCGGGCCG‑CGCGGCTGATc                                                                                                               <  2:281977/137‑1 (MQ=255)
            cAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTG‑CTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCG‑CGCGGCTGATCg                                                                                                              <  6:331363/137‑1 (MQ=255)
                  ccTCGGACTGCCGTGCATCCCGGAGAGGGCAGAAACGGTTGCCCGGGGGCTCAACTTGCTGAGTGGT‑GATCATAGCGCCGGGCGTGGGCAGTTCAGCGGGTGCACCCGCGCCCGTGGGG‑CGCGGCTGATCgcccagg                                                                                                        <  8:352906/137‑1 (MQ=255)
                            ccGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTG‑CTGGTGGAACAGAGCGGCGTGCGGGCGCGCTTGAGCCCCCTCACCCGCGCCCGTGGCG‑CGCGGGGGGTCGCCccggccccggccgg                                                                                              >  3:61194/1‑135 (MQ=255)
                                     ccGGCGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTG‑CTGGTGGAACAGAGCGGCGGGCGTGCGCACTTCACCCAGATCACCAGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGcc                                                                                     <  8:253947/137‑1 (MQ=255)
                                      cGGAGCGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTG‑CTGGTGCAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCg                                                                                    <  2:69346/137‑1 (MQ=255)
                                         agagCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTG‑CTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCG‑CGCGGCTGATCGCCCCGGCCCAGGCCTTGGGCCTGCCGGTa                                                                                 >  6:99744/1‑137 (MQ=255)
                                                cGAAACCGTGGCCCTGGCGCGCAACCTGCTG‑CTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                          <  2:88906/137‑1 (MQ=255)
                                                 gAAACCGTGGCCCTGGCGCGCAACCTGCTG‑CTGGTGGAACAGAGCGGCGTGCGGGCGCACTTCAGCCCGATCACCCGCGCCCCTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCGTGGGCCTGCCGGTAAccgccgc                                                                         >  5:321681/1‑136 (MQ=255)
                                                     gcggggCCGTGGCGGGCACCATGGTG‑GGGGTGCAACAGTGGGGCGTGCGTGCGCAGTTCCGCCAGATCACCAGCGCCGGTGGGG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                     <  4:17209/133‑1 (MQ=255)
                                                     ccGTGGCCCTGGCGCGCACCCTGCTG‑CTGGTGCAACAGAGCGGCGTGCGTGCGCACTTCAGCCATATCACCAGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                     <  8:283093/137‑1 (MQ=255)
                                                     ccGTGGCCCTGGCGCGCAACCTGCTG‑CTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                     <  4:268183/137‑1 (MQ=255)
                                                     ccGTGGCCCTGGCGCGCAACCTGCTG‑CTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                     <  5:340479/137‑1 (MQ=255)
                                                     ccGTGGCCCTGGCGCGCAACCTGCTG‑CGGGTGGAACAGAGCGGGGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                     <  3:180781/137‑1 (MQ=255)
                                                     ccGTGGCCCGGGCGCGCAACCGGCGG‑CTGGTGGAACAGAGCGGCGTGCGTGCGAACTTCAGCCAGATCACCCGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                     <  2:229509/137‑1 (MQ=255)
                                                     ccGGGGCCCTGGCGCGAAACCTGCTG‑CTGGTGGACCAGAGCGGCGGGCGTGCGAACTTCAGCCAGATCCCCCGCGCCCGGGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                     <  7:232917/137‑1 (MQ=255)
                                                      gggggCCCTGGCGCGCAACCTGCTG‑CTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAg                                                                    <  7:294569/134‑1 (MQ=255)
                                                          ccccTGGCGCGCAACCTGCTG‑CTGGTGGAACAGAGCGGCGGGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCt                                                                <  6:134632/136‑1 (MQ=255)
                                                           cccGGGCGCGCAACCTGCTG‑CTGGGGGAACAGAGCGGCGTGCGTGCGCAGTTCAGCCAGATCCCCAGCGCCGGGGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTg                                                               <  4:230016/137‑1 (MQ=255)
                                                                 cgcgcAACCTGCTG‑CTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAg                                                         <  8:85968/137‑1 (MQ=255)
                                                                 cgcgcAACCTGCTG‑CTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAg                                                         <  2:32246/137‑1 (MQ=255)
                                                                 cgcgcAACCTGCTG‑CTGGTGGAAAAGAGCGGCGTGCGTGCGCACTTCAGCCAGAGCACCAGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAg                                                         <  2:312494/137‑1 (MQ=255)
                                                                 cgcgcAACCTGCGG‑GTGGTGCAACCGCGCGGCGTGCGTGCGCAGTTCAGCCAGATCACCCCCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAg                                                         <  4:18175/137‑1 (MQ=255)
                                                                   cgcAACCTGCTG‑CTGGTGGAACAGAGCGGCGGGCGGGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCt                                                       <  6:296531/137‑1 (MQ=255)
                                                                        ccTGCTG‑CTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCCTGGCG‑CGCGGCGGATCCCCCCCGCCCCGGCCCTGGGCCTGCCGGTAACCCCCCAGGTACCGCTGTACCCGCTGATcc                                                  >  8:31920/1‑137 (MQ=255)
                                                                        ccTGCTG‑CTGGTGGAACAGAGCGGCGTGAGTGCGCAATTAAGCCAGCTAACCAGCGCCCGTTGCG‑CGCGGCTTAGAGCCCCGGCCCACGGCATGGGCCTGCCCGTTACCGCACATTTTGCGCGTGACCAACTGATcg                                                  >  8:372316/1‑136 (MQ=255)
                                                                         cggctg‑cggGTGGAACAGCGCGGCGGGCGTGCGCACTCCAGCCAGACCACCCGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCt                                                 <  2:334339/135‑1 (MQ=255)
                                                                                gtggtggAACAGCGGGGGGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGa                                           <  2:41949/136‑1 (MQ=255)
                                                                                            ggcggcgGGCGGGCGCACTCCCGCCATACCCCCCGCGCCCG‑GGCGCCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                               <  6:255053/136‑1 (MQ=255)
                                                                                            cgcgccgTGCGTGCGCACTTCACCCATAACACCCGCGCCCGTGCCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                               <  6:299556/136‑1 (MQ=255)
                                                                                             gcggggTGCGTGCGCACTTCAGCCAGATCACCTGCGCCGGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcgc                              <  6:337672/137‑1 (MQ=255)
                                                                                             gcggcgTGCGTGCGAACTTCAGCCAGATCACCCGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCGGTACCAGCTGATCCTCACGGACGAGTCGCTgcgc                              <  8:296921/137‑1 (MQ=255)
                                                                                                 ggtgcgtgcGCACTTCAGCCAGATCACCAGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                          <  3:186692/136‑1 (MQ=255)
                                                                                                 ggtgcgtgcGCACTTCACCCATAGCACCCGCGCCGGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                          <  1:99484/136‑1 (MQ=255)
                                                                                                 ggtgcgtgcCCACTTCCGCAAGATCACCAGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                          <  7:293840/136‑1 (MQ=255)
                                                                                                 ggtgcgggcGCACTTCCGCCAGATCACCAGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                          <  6:321681/136‑1 (MQ=255)
                                                                                                 ggggcgggcgCACTTCCGCCAGATCACCCGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                          <  1:97700/134‑1 (MQ=255)
                                                                                                  gggcgtgcgCAGTGCAGCACTATCACCTGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTTACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGtt                         <  1:329828/135‑1 (MQ=255)
                                                                                                   tgcgtgcgCACTTCAGCCAGATCACCAGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTtc                        <  6:287333/137‑1 (MQ=255)
                                                                                                        gcgcACTTCAGCCAGATCACCAGCGCCCGTGGCG‑CGCGGCTGATAGCCCACGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTATCGCTGTACCAGCTTATACTCACGGACGAGTCGCTGCGCGAGTTATCCac                   >  5:242383/1‑136 (MQ=255)
                                                                                                            aCTTCAGCCAGATCACCAGCGCCCGTAGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTg               >  1:297169/1‑137 (MQ=255)
                                                                                                                      gATCACCAGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                          <  6:170605/68‑1 (MQ=255)
                                                                                                                      gATCACCAGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                          >  5:170605/1‑68 (MQ=255)
                                                                                                                         cACCAGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAgc  >  1:54698/1‑137 (MQ=255)
                                                                                                                             |                                                                                                                                     
AGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTG‑CTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCG‑CGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGC  >  ACB122/5688513‑5688769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑