| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 6,038,658 | 0 | C | A | 21.5% | 146.0 / 27.2 | 93 | A37S (GCC→TCC) | puuD_3 | Gamma‑glutamyl‑gamma‑aminobutyrate hydrolasePuuD |
| Reads supporting (aligned to +/- strand): ref base C (29/44); new base A (0/20); total (29/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.45e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGGGCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCA > ACB122/6038527‑6038791 | gggggCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑gc > 1:367429/1‑137 (MQ=255) gcgcCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGGCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑gcc‑ggc < 7:247802/137‑1 (MQ=255) cccATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑gcg‑ggccg < 1:307752/136‑1 (MQ=255) cccATTGTAATGATGCGGCGCGAAAGTTGCAGCCGAGCCGGAAAAAACAATGCCGGGCCGGCGAGCCAGCAGCGCTTGCGGGTCGCGGCCGCCATCACGGGCGGGCAAGACCTGTGCCAAGCCGCAAAA‑gcc‑ggccg < 4:324904/136‑1 (MQ=255) cccATGGAAATGATGCGGCGCGATATTTGACGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑gcc‑ggccg < 8:118716/136‑1 (MQ=255) aTTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCt > 1:29999/1‑137 (MQ=255) aaTGATGCGGCTCTATATTTGACGGCGAGCCGTAAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGGGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACa < 5:140295/137‑1 (MQ=255) aaTGATGCGGCTCGATATTTGACGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACa < 7:115159/137‑1 (MQ=255) cGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGt > 4:345468/1‑137 (MQ=255) cGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGt > 5:208191/1‑137 (MQ=255) cGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGt > 3:116319/1‑137 (MQ=255) cGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGt > 4:124528/1‑137 (MQ=255) cGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTAGACATACTTGt > 4:138924/1‑137 (MQ=255) ggCTCTATATTTTAAGGCGAGCCGGTAAAAACATTGCGGTGCTGGCGAGCCAGCAGCGCTTGCGGGGCGCTGCCGCCATCAGGGGCGGGCAATATCAGGGGCACGCCGGAAAA‑GCC‑GGCCGCCTCGACATACTTGTc < 8:168128/137‑1 (MQ=255) gCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCg > 7:238845/1‑137 (MQ=255) cTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGc > 3:169141/1‑137 (MQ=255) cTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGc > 3:97712/1‑137 (MQ=255) cTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGc > 7:178605/1‑137 (MQ=255) cTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGc > 7:67569/1‑137 (MQ=255) cTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGc > 1:24220/1‑137 (MQ=255) cTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGGCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCACGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGc < 7:280642/137‑1 (MQ=255) tCGATATTTGACGGGGTGCCTTTAAAAAAAAGGCCGTCCAGGCTTGCCTGCAGCTCTTTCTTGGCGGGGCGGCAATCAGGGGCCGTAAAGAGATGTGCCAAG‑CGGCAAATGCG‑TGCCGCCTCGACATACTTGTCGcc < 4:77207/137‑1 (MQ=255) tCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGcc < 8:301365/137‑1 (MQ=255) cGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGccc > 5:253086/1‑136 (MQ=255) cGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCg > 4:364283/1‑137 (MQ=255) tGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGAAAGATCAGTGGCAAGCCGGAAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCgtgt < 2:367429/137‑1 (MQ=255) ccgccGAGCCGGTAAAAAAAATGCCGTGCTGGCGACCCAGCAGCGCTGGCGGGGCGCTGCCCCCAACCCGGGCCGGCAAGAGCCGTGGCAAGCCGGAAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGc < 4:319456/133‑1 (MQ=255) gAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCgagga > 7:326623/1‑137 (MQ=255) gCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGt > 4:241211/1‑137 (MQ=255) gCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGt > 5:392917/1‑137 (MQ=255) cccGGTAAAAACAATGCCGTCCAGGCGAGCCAGCAGCGCTTGCGTGGCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGCCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGt < 5:107706/136‑1 (MQ=255) aaTGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAATATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACct < 6:130133/137‑1 (MQ=255) aaTGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCAATCACGGGCCGGCAAGATCAGTGCCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACct < 3:184560/137‑1 (MQ=255) ccGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACctgctg > 3:208331/1‑137 (MQ=255) cGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGCCCGTGTGCGAGGAGTTCTTCCCTACCTGCTGa > 3:15774/1‑137 (MQ=255) cGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGa > 7:285709/1‑137 (MQ=255) cGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGa > 6:89019/1‑137 (MQ=255) tGCCGGCTAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGAAATATCAGTGGCACGCCGGAAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 8:49402/137‑1 (MQ=255) tGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 6:161599/137‑1 (MQ=255) ggCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 4:182208/136‑1 (MQ=255) ggCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 6:151758/136‑1 (MQ=255) gccAGGCTAGCCAGCCGCGCTTGCGGTGCGCTGCCGCCAACTCGGGCGGCCAAGATAAGTGGCAAGCGGGAAAA‑GCG‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 8:360986/135‑1 (MQ=255) aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGTCATAGTTGTCGCCGCCCGTGTGCGAGGAGGTCTTCCCTACCTTcggccggcc > 1:169303/1‑136 (MQ=255) aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCa > 3:144488/1‑137 (MQ=255) aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCa > 4:105256/1‑137 (MQ=255) aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAACATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGCCATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCAGCTCACGGCa > 3:186324/1‑137 (MQ=255) cagctgCGGTTGCGTGTCGATGCCGCCATCACGGGCCGGAAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGa < 2:285660/137‑1 (MQ=255) cagcagCGGTTGGTGGTCGCTGCCCCCATCAGGGGCCGCAAATATAAGTGGAACCCCGGCAAC‑GCG‑GCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGa < 3:241211/137‑1 (MQ=255) cagcagCGCTGGCGGGTCGCGGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGAAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGa < 4:169141/137‑1 (MQ=255) aagcaggGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGa < 2:29999/136‑1 (MQ=255) aagcagcGCTTGCGTGTCGCTGCCGCCATAAGGGGCCGGCAATATCAGTGGAAAGCCGGAAAA‑GCG‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGa < 5:303204/136‑1 (MQ=255) gcagcGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGaca < 2:24220/137‑1 (MQ=255) ggCGGGTCGCGGCCGCATCCAGGGGCCGGCAAGATCTGTGCCACCCCGGAAAA‑GCG‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCaa < 3:247140/136‑1 (MQ=255) gtgtCGCTGCCGCCATCACGGGCCGGAAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAgg < 3:72640/137‑1 (MQ=255) gccgccATCGCGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCgagg < 4:175547/83‑1 (MQ=255) gccgccATCGCGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCgagg > 3:175547/1‑83 (MQ=255) gccgccATCACGGGCCGGCAAGATAAGTGGCAAGCCGGAAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCAt < 4:151791/137‑1 (MQ=255) cccgccATCACGGGCCGGCAAGATCAGTGGCACGCCGTCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTACCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCAt < 7:380590/136‑1 (MQ=255) gccATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTg > 3:248458/1‑137 (MQ=255) atctggggCGGGAAAGATCAGTGGCAAGCCGGAAAT‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:208104/132‑1 (MQ=255) atccggggCCGGCATGATCTGTTCAACGCCGCAAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 5:6123/132‑1 (MQ=255) ataccGGGCCGGAAATATCAGTGGCAAGCCGGAAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 3:128180/133‑1 (MQ=255) ataaCGGGCGGGAATTATCAGTGGCAAGCGGGATAA‑GCG‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTTCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 4:186324/134‑1 (MQ=255) accccGGGCCGGAATGATCAGTGCCACGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 5:253080/133‑1 (MQ=255) aTCCCGGGCCGGAAAGATCAGTGGCAAGCCGGAAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:322819/137‑1 (MQ=255) aTCAGGGGCCGGAAATATCAGGGGCAAGCCGGCAAT‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:208191/137‑1 (MQ=255) aTCACGGGCGGGCAAGAGCTGTGGCACGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 5:57368/137‑1 (MQ=255) aTCACGGGCCGGCATGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 7:82869/137‑1 (MQ=255) aTCACGGGCCGGCACGATCAGTGGCAAGCCGGAAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 3:127572/137‑1 (MQ=255) aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCT‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 7:29858/137‑1 (MQ=255) aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 8:178605/137‑1 (MQ=255) aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 4:116319/137‑1 (MQ=255) aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 8:285709/137‑1 (MQ=255) aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 1:53185/137‑1 (MQ=255) aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 3:364283/137‑1 (MQ=255) aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:87556/137‑1 (MQ=255) aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:260755/137‑1 (MQ=255) aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 5:110240/137‑1 (MQ=255) aTCACGGGCCGGCAAGATCAGTGGCAAGCCGGAAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 3:265922/137‑1 (MQ=255) aTCACGGGCCGGCAAGATCAGTGGAAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 7:285666/137‑1 (MQ=255) aTCACGGGCCGGAAATATCAGTGGCAAGCCGGAAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 7:86215/137‑1 (MQ=255) ggCAATATCAGTGGCAAGCCGGAAAA‑GCG‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCtt < 5:133879/137‑1 (MQ=255) ggCAAGATCAGTGGCATGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCtt < 5:259321/137‑1 (MQ=255) ggCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCtt < 4:144488/137‑1 (MQ=255) ggCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCtt < 5:89019/137‑1 (MQ=255) ggCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCtt < 3:138924/137‑1 (MQ=255) cAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTac < 2:60825/137‑1 (MQ=255) aaGATCAGTGGCAAGCCGGAAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTaca < 8:67569/137‑1 (MQ=255) cAGTGGCAAGCCGGCAAA‑GCCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgt < 5:19611/137‑1 (MQ=255) cAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgtg < 6:379893/137‑1 (MQ=255) gggccAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGtgtg < 3:42898/133‑1 (MQ=255) ggCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCa < 4:208331/137‑1 (MQ=255) cGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCa > 7:206638/1‑137 (MQ=255) | GGGGGCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCC‑GGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTACCA > ACB122/6038527‑6038791 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |