Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB122	2,242,308	G→T	S116R (AGC→AGA)	CJ019_02129 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	2,242,308	0	G	T	98.0%	174.6 / NA	51	S116R (AGC→AGA)	CJ019_02129	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (25/25);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGAAGCGCTTTTCCGAATTGATCAGCGCCGTTTCCCGTTCGCGCTGGCGGGTGATCTCGCGGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGGCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTGACCACCGCCTGGAACACCTGGCGCGTGCGGGCGCGATCCTCCTCGGGCAGCAGGTCGAGGTAATCGATAT  >  ACB122/2242174‑2242435                                                                                                                                       |                                                                                                                                gCGAAGCGCTTTTCCGAATTGATCAGCGCCGTTTCCCGTTCGCGCTGGCGGGTGATCTCGCGGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCt                                                                                                                               >  1:110701/1‑137 (MQ=255) gCGAAGCGCTTTTCCGAATTGATCAGCGCCGTTTCCCGTTCGCGCTGGCGGGTGATCTCGCGGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCt                                                                                                                               >  4:272914/1‑137 (MQ=255) gCGAAGCGCTTTTCCGAATTGATCAGCGCCGTTTCCCGTTCGCGCTGGCGGGTGATCTCGCGGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCt                                                                                                                               >  3:70347/1‑137 (MQ=255) gCGAAGCGCTTTTCCGAATTGATCAGCGCCGTTTCCCGTTCGCGCTGGCGGGTGATCTCGCGGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCt                                                                                                                               >  3:31734/1‑137 (MQ=255) gCGAAGCGCTTTTCCGAATTGATCAGCGCCGTTTCCCGTTCGCGCTGGCGGGTGATCTCGCGGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCt                                                                                                                               >  7:6575/1‑137 (MQ=255) gCGAAGCGCTTTTCCGAATTGATCAGCGCCGTTTCCCGTTCGCGCTGGCGGGTGATCTCGCGGATAACGCCGATCATCTGCGGTCGGCAGTGCGGGTCGTGGGTCAGGCTGCCGTTGATATACAGCCAGTGCAGTCt                                                                                                                               >  3:93665/1‑137 (MQ=255)    aaGCGCTTTTCCGAATTGATCAGCGCCGTTTCCCGTTCGCGCTGGCGGGTGATCTCGCGGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGcc                                                                                                                            <  3:232197/137‑1 (MQ=255)         cTTTTCCGAATTGATCAGCGCCGTTTCCCGTTCGCGCTGGCGGGTGATCTCGCGGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCg                                                                                                                       <  4:49328/137‑1 (MQ=255)         cTTTTCCGAATTGATCAGCGCCGTTTCCCGTTCGCGCTGGCGGGTGATCTCGCGGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCg                                                                                                                       <  4:175763/137‑1 (MQ=255)          ttttCCGAATTGATCAGCGCCGTTTCCCGTTCGCGCTGGCGGGTGATCTCGCGGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCgg                                                                                                                      <  5:255029/137‑1 (MQ=255)           tttCCGAATTGATCAGCGCCGTTTCCCGTTCGCGCTGGCGGGTGATCTCGCGGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGc                                                                                                                     <  6:105875/137‑1 (MQ=255)              ccGAATTGATCAGCGCCGTTTCCCGTTCGCGCTGGCGGGTGATCTCGCGGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAg                                                                                                                  <  4:225617/137‑1 (MQ=255)               cGAATTGATCAGCGCCGTTTCCCGTTCGCGCTGGCGGGTGATCTCGCGGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGc                                                                                                                 <  2:110701/137‑1 (MQ=255)               cGAATTGATCAGCGCCGTTTCCCGTTCGCGCTGGCGGGTGATCTCGCGGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGc                                                                                                                 <  5:352607/137‑1 (MQ=255)                 aaTTGATCAGCGCCGTTTCCCGTTCGCGCTGGCGGGTGATCTCGCGGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCgg                                                                                                               <  1:258439/137‑1 (MQ=255)                   ttGATCAGCGCCGTTTCCCGTTCGCGCTGGCGGGTGATCTCGCGGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGAt                                                                                                             <  6:185850/137‑1 (MQ=255)                    tGATCCGCGCCGTTTCCCGTTCGCGCTGGCGGGTGAGCTCGCGGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATa                                                                                                            <  2:361687/137‑1 (MQ=255)                    tGATCAGCGCCGTTTCCCGTTCGCGCTGGCGGGTGATCTCGCGGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATa                                                                                                            <  3:272914/137‑1 (MQ=255)                    tGATCAGCGCCGTTTCCCGTTCGCGCTGGCGGGTGATCTCGCGGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATa                                                                                                            <  6:350361/137‑1 (MQ=255)                          gcgcCCTTTCCCGTTCGCGCTGGCGGGTGATCTCGCGGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTgg                                                                                                      <  4:172051/137‑1 (MQ=255)                                   cccGTTCGCGCTGGCGGGTGATCTCGCGGATCACGCCGATCATCTTCGGTCGGCCGTGCGGGTCGTGGGTTAGGCTGCCGTTGATCTACAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCt                                                                                             >  2:335355/1‑137 (MQ=255)                                             cTGGCGGGTGATCTCGCGGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCg                                                                                   <  2:229964/137‑1 (MQ=255)                                                         ctcGCGGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTgc                                                                       >  1:63358/1‑136 (MQ=255)                                                         ctcGCGGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTGa                                                                       <  3:57527/137‑1 (MQ=255)                                                             cgGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCCGCCAGTGCAGTCTGCCCTCCGGCCAGCGGATACGGTGGCGCAAGGGTTGCTCCAACGGCTCGCCCTTGacccc                                                                   >  6:249770/1‑135 (MQ=255)                                                                aTCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCCTTGACCACCGc                                                                >  7:146245/1‑137 (MQ=255)                                                                aTCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTGACCACCGc                                                                >  2:351830/1‑137 (MQ=255)                                                                 ccACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTGACCACCGcc                                                               <  6:280595/136‑1 (MQ=255)                                                                    cGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTGACCACCGCCTgg                                                            >  4:77418/1‑137 (MQ=255)                                                                             tcTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTGACCACCGCCTGGAACACCTgg                                                   >  5:156682/1‑137 (MQ=255)                                                                             tcTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTGACCACCGCCTGGAACACCTgg                                                   >  5:98811/1‑137 (MQ=255)                                                                             tcTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCCTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGCTACGGTGGCGCATGGCTTGCTCCACCGGCTAGCCATTGACCCCCGCCTGGCACACCAgg                                                   >  6:272010/1‑137 (MQ=255)                                                                                gCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTGACCACCGCCTGGAACACCTGgcgc                                                <  8:349732/137‑1 (MQ=255)                                                                                 cGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTGACCACCGCCTGGAACACCTGGcgcg                                               <  2:22665/137‑1 (MQ=255)                                                                                              gggTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTGACCACCGCCTGGAACACCTGGCGCGTGCGGGCGCGAtc                                  >  6:238197/1‑137 (MQ=255)                                                                                                gTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTGACCACCGCCTGGAACACCTGGCGCGTGCGGGCGCGAtcct                                <  1:105412/137‑1 (MQ=255)                                                                                                     gggTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTGACCACCGCCTGGAACACCTGGCGCGTGCGGGCGCGCTCCTCCTCg                           >  7:286390/1‑137 (MQ=255)                                                                                                     gggTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTGACCACCGCCTGGAACACCTGGCGCGTGCGGGCGCGATCCTCCTCg                           >  4:317563/1‑137 (MQ=255)                                                                                                       gTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTGACCACCGCCTGGAACACCTGGCGCGTGCGGGCGCGATCCTCCTCggg                         <  1:112306/137‑1 (MQ=255)                                                                                                       gTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTGACCACCGCCTGGAACACCTGGCGCGTGCGGGCGCGATCCTCCTCggg                         <  5:308279/137‑1 (MQ=255)                                                                                                        tCAGGCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTGACCACCGCCTGGAACACCTGGCGCGTGCGGGCGCGATCCTCCTCGGgc                        >  7:96337/1‑137 (MQ=255)                                                                                                            gCTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCAGCGGCTCGCCATTGACCACCGCCTGGAACACGTGGCGCGTGCGGGCGCGATCCTCCTCGGgctgca                    <  5:249770/137‑1 (MQ=255)                                                                                                             cTGCCGTTGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGAATGGCTTGCTCCACCGGCTCGCCATTGACCACCGCCTGGAACACCTGGCGCGTGCGGGCGCGATCCTCCTCGTGcagcag                   >  5:120177/1‑137 (MQ=255)                                                                                                             cTGCCGTTGATATCCATCCAGTGCAGTCTGCCGTCCGGCCATCTGATAAGGTTGCGCAAGGCTTTCTGAACCGGCTCGCCATTGACCAACGCATTGAACACCTGGCGCGTGCGGGCGGCTTACTCCTCGGgcagctt                   >  2:137683/1‑135 (MQ=255)                                                                                                                   ttaaTCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTGACCACCGcc                                                               >  7:244245/4‑87 (MQ=255)                                                                                                                   ttaaTCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTGACCACCGcc                                                               <  8:244245/84‑1 (MQ=255)                                                                                                                   ttGATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTGACCACCGCCTGGAACACCTGGCGCGTGCGGGCGCGATCCTCCTCGGGCAGCAGGTCGAg             >  3:358493/1‑137 (MQ=255)                                                                                                                     gATCTCCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTGACCACCGCCTGGAACACCTGGCGCGTGCGGGCGCGATCCTCCTCGGGCAGCAGGTCGAGGt           <  6:156682/137‑1 (MQ=255)                                                                                                                         tcCAGCCAGTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTGACCACCGCCTGGAACACCTGGCGCGTGCGGGCGCGATCCTCCTCGGGCAGCAGGTCGAGGTAATc       >  2:373811/1‑137 (MQ=255)                                                                                                                             gccagTGCAGTCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTGACCACCGCCTGGAACACCTGGCGCGTGCGGGCGCGATCCTCCTCGGGCAGCAGGTCGAGGTAATCGata   >  6:270061/1‑137 (MQ=255)                                                                                                                              ccagTGCAGGCTGCCGTCCGGCCAGAGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTGACCACCGACTGGAACACCTGGCGCGTGCGGGCGCGATCCTCCTCGGGCAGCAGGGCGAGGTAATCGatat  <  1:325343/137‑1 (MQ=255)                                                                                                                                       |                                                                                                                                GCGAAGCGCTTTTCCGAATTGATCAGCGCCGTTTCCCGTTCGCGCTGGCGGGTGATCTCGCGGATCACGCCGATCATCTGCGGTCGGCCGTGCGGGTCGTGGGTCAGGCTGCCGTTGATCTCCAGCCAGTGCAGGCTGCCGTCCGGCCAGCGGATACGGTGGCGCATGGCTTGCTCCACCGGCTCGCCATTGACCACCGCCTGGAACACCTGGCGCGTGCGGGCGCGATCCTCCTCGGGCAGCAGGTCGAGGTAATCGATAT  >  ACB122/2242174‑2242435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑