Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB122	3,065,508	G→A	V53M (GTG→ATG)	rcsC_6 →	Sensor histidine kinase RcsC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	3,065,508	0	G	A	100.0%	202.5 / NA	56	V53M (GTG→ATG)	rcsC_6	Sensor histidine kinase RcsC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/28);  total (28/28)

AGCAGCGGGCGTTGGCCGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCGTGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGA  >  ACB122/3065374‑3065641                                                                                                                                       |                                                                                                                                      agcagcGGGCGTTGGCCGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAACACGCCATg                                                                                                                                     >  7:142683/1‑137 (MQ=255) agcagcGGGCGTTGGCCGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATg                                                                                                                                     >  3:215270/1‑137 (MQ=255)   cagcGGGCGTTGGCCGGGCTGCTGGCGCTGCGTGACCACTCGGCGCGTAAAAGACATTACCCGGAGCTGTCCCCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGATCTTCGAAAACCCCATGcc                                                                                                                                   >  8:387032/1‑136 (MQ=255)      cGGGCGTTGGCCGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAACACGCCATGCACgg                                                                                                                                >  6:354683/1‑137 (MQ=255)             tGGCCGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATT‑CCCGGAGTTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTcc                                                                                                                        >  6:376925/1‑137 (MQ=255)              ggCCGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTcc                                                                                                                        >  5:230372/1‑137 (MQ=255)               gCCGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCa                                                                                                                       <  7:338474/137‑1 (MQ=255)               gCCGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCa                                                                                                                       >  5:275464/1‑137 (MQ=255)               gCCGGGCGGCGGGGGCTGGGGGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACATATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCa                                                                                                                       <  2:132143/137‑1 (MQ=255)                ccGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAg                                                                                                                      >  2:281311/1‑137 (MQ=255)                 cGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAgg                                                                                                                     <  8:170924/137‑1 (MQ=255)                 cGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAgg                                                                                                                     <  6:142273/137‑1 (MQ=255)                   ggCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGcc                                                                                                                   <  6:72739/137‑1 (MQ=255)                      tgctgGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGTCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGc                                                                                                                >  2:295649/1‑137 (MQ=255)                       gctggggctgggTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGcc                                                                                                               >  2:123831/1‑137 (MQ=255)                         tggggctgggTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCtt                                                                                                             <  1:308816/137‑1 (MQ=255)                         tggggctgggTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCAGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCtt                                                                                                             <  4:69857/137‑1 (MQ=255)                                        aCTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGt                                                                                              >  5:68665/1‑137 (MQ=255)                                         cTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTg                                                                                             >  5:389334/1‑137 (MQ=255)                                               gcgTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCa                                                                                       >  1:152135/1‑137 (MQ=255)                                               gcgTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCa                                                                                       >  7:340823/1‑137 (MQ=255)                                               gcgTAAAAGCCATTACCCGGAGCTGTCCCCCCGCCTGGACGAACTCGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACCGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCa                                                                                       >  6:62235/1‑137 (MQ=255)                                                   aaaaGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCATCCTTCAGGACGGCATGCGTGCCGCCAAccc                                                                                   >  7:353818/1‑137 (MQ=255)                                                           ttACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTgg                                                                           <  4:325387/137‑1 (MQ=255)                                                                 ggAGCTGTGCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCa                                                                     <  2:40202/137‑1 (MQ=255)                                                                  taGCTGTCCGCCCGCGTGGACGAACTGGAAGCCGAACGCAACCTTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGAATGCGTGCCGCCAACCCGGCGCTGGCGCGCAt                                                                    <  6:275464/136‑1 (MQ=255)                                                                  gAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCAt                                                                    <  7:294370/137‑1 (MQ=255)                                                                  gAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCAt                                                                    <  4:27947/137‑1 (MQ=255)                                                                  gAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCAt                                                                    <  1:281311/137‑1 (MQ=255)                                                                  gAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCAt                                                                    >  1:303979/1‑137 (MQ=255)                                                                  gAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCAt                                                                    <  4:215270/137‑1 (MQ=255)                                                                     cTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCt                                                                 >  5:338957/1‑137 (MQ=255)                                                                      tGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTc                                                                >  4:84007/1‑137 (MQ=255)                                                                           gcccgccTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCAt                                                                                                  >  8:100246/1‑98 (MQ=255)                                                                           gcccgccTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCAt                                                                                                  <  7:100246/98‑1 (MQ=255)                                                                           gcccgccTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTa                                                           >  4:14316/1‑137 (MQ=255)                                                                           gcccgccTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTa                                                           >  8:143028/1‑137 (MQ=255)                                                                            cccgccTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTAt                                                          <  5:391446/137‑1 (MQ=255)                                                                              cgccTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATga                                                        <  8:191160/137‑1 (MQ=255)                                                                                  tGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATgacgac                                                    >  3:38950/1‑137 (MQ=255)                                                                                      cGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGc                                                <  3:66359/137‑1 (MQ=255)                                                                                            ggAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCgg                                          <  7:97027/137‑1 (MQ=255)                                                                                            ggAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCgg                                          <  5:245881/137‑1 (MQ=255)                                                                                                   gAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGtt                                   <  1:123831/137‑1 (MQ=255)                                                                                                     aCGCAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGtttt                                 >  6:44590/1‑137 (MQ=255)                                                                                                        cAACCGTTACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCAGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTccc                              <  5:196578/137‑1 (MQ=255)                                                                                                               tACAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCctgactga                       <  5:4780/137‑1 (MQ=255)                                                                                                                 cAAATGGCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGAcc                     >  8:281302/1‑137 (MQ=255)                                                                                                                       gCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGgccg               <  5:313293/137‑1 (MQ=255)                                                                                                                       gCTGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGgccg               >  5:273015/1‑137 (MQ=255)                                                                                                                         tGTTCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGgccgcc             <  1:295649/137‑1 (MQ=255)                                                                                                                           ttCGAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCaa           >  2:199559/1‑137 (MQ=255)                                                                                                                              gAAAACGCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCt        >  3:84620/1‑137 (MQ=255)                                                                                                                                    gCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGa  <  7:9510/137‑1 (MQ=255)                                                                                                                                    gCCATGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGa  <  5:354683/137‑1 (MQ=255)                                                                                                                                    gCCATGCAAGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAAACCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGa  <  6:319911/137‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      AGCAGCGGGCGTTGGCCGGGCTGCTGGGGCTGGGTGACCACTCGGCGCGTAAAAGCCATTACCCGGAGCTGTCCGCCCGCCTGGACGAACTGGAAGCCGAACGCAACCGTTACAAATGGCTGTTCGAAAACGCCGTGCACGGTATTTTCCAGGCCAGCCTTCAGGACGGCATGCGTGCCGCCAACCCGGCGCTGGCGCGCATGCTCGGCTATGACGACCCGCAAGCGGTGCTGTTTTCCCTGACTGACCTGGCCGCCAACCTGTTTGA  >  ACB122/3065374‑3065641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑