| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 120,975 | Δ1 bp | 100% | intergenic (+272/+100) | polA → / ← engB | DNA polymerase I/putative GTP‑binding protein EngB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 120,975 | 0 | T | . | 95.7% | 294.6 / 4.7 | 69 | intergenic (+272/+100) | polA/engB | DNA polymerase I/putative GTP‑binding protein EngB |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base . (51/15); total (53/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
GGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTTCCATCC > ACB122/120857‑121106 | ggCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAAtt > 7:69476/1‑136 (MQ=255) aaGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TGTTTTTTGCCCGGAATTTGCCg > 3:74673/1‑133 (MQ=255) aaGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCAGACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCg > 3:28895/1‑133 (MQ=255) aGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAg < 8:233482/136‑1 (MQ=255) ccTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCGCGGAATTTGCCGGGAGCg > 6:243707/1‑135 (MQ=255) cGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGtt > 2:218349/1‑135 (MQ=255) aTGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGaa > 2:213290/1‑135 (MQ=255) tgttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt < 7:2502/135‑1 (MQ=255) gttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt < 6:306304/134‑1 (MQ=255) gttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt < 1:6094/134‑1 (MQ=255) ttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGc < 8:14013/135‑1 (MQ=255) tgtgTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCg < 6:128793/135‑1 (MQ=255) tgtgTTAAGTGGTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCg < 1:323488/135‑1 (MQ=255) ttAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTc > 7:90072/1‑136 (MQ=255) tatgtgtTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGACGGGAGCGGGTTTTCTCGCGAATGCG‑ATGt > 3:144217/4‑134 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCTGACTTGGACAACGAACTTCCCCCTCACAATACTAAGTCCGGGG‑TTTTTTTTGCCAGGAATTTGAAGGGAGCTGGTTTTCTCGCGAATGCG‑At > 5:130668/1‑132 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGt > 2:149283/1‑134 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGt > 3:209217/1‑134 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGt > 4:153588/1‑134 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGt > 1:27983/1‑134 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGt > 1:268113/1‑134 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTc > 8:127754/1‑135 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTc > 8:227688/1‑135 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGAG‑ATGTc > 1:183256/1‑135 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCAGACGAAGGCCGGGG‑TTTTTTTTGCCCGGAAGTTGCCGGGAGCGGGTTTTCTCGCGCAGGCG‑ATGTc > 3:264628/1‑135 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGACCGGACTTGGACCCCGAACTTCCCCCTCCCAATACGAAGTCCGGGG‑TTTTTTTTGCCCTGAATTTGACGGGAGCGGGTTTTCTCCCGCATGCG‑ATGt > 6:32105/1‑134 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCATAGCGGTCCGGACTTGGACCCCGAACTTCCCCATCCCCATACGAAGTCCGGGGTTTTTTTTT‑CCCCGAATTTTCCGGGAGCGGGTTTTCGCGCGAATGCG‑CTGt > 3:133782/1‑134 (MQ=255) tAAGTGTTGGCAGATATCTGAACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt < 5:132319/127‑1 (MQ=255) tAAGTGTTGGCAGATATCTGAACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt > 6:132319/1‑127 (MQ=255) aGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCTGGAATTTGACGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGa > 3:142214/1‑135 (MQ=255) aGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCg > 1:1704/1‑134 (MQ=255) aGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGa > 3:119600/1‑135 (MQ=255) gtaggCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCAGCGG‑GATTATTTGCCCGGAATTTGCCGGGAGCGGGATTTCAAGCGAAAGCG‑ATCTCGAAt > 5:183551/4‑134 (MQ=255) tGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATaca < 6:184365/135‑1 (MQ=255) cAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACAccgc > 8:296171/1‑136 (MQ=255) gATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACAccgcc > 3:293888/1‑135 (MQ=255) gATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACAccgcc > 4:375892/1‑135 (MQ=255) gATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACAccgcc > 2:373357/1‑135 (MQ=255) cTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCAtt > 1:20753/1‑135 (MQ=255) cTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCAtt > 5:159824/1‑135 (MQ=255) ggACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCATTcg > 4:168380/1‑135 (MQ=255) cccccGCCCTCCCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCATTcg < 5:35091/132‑1 (MQ=255) aCCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑GTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGGGGATTACCCCGCCGCATTcgcg > 3:250465/1‑135 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGCGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCATTCGCGGGTAAAcc > 7:249151/1‑136 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGCATGCG‑ATGTCGAATACACCGCCGCATTCGCGGGTAAAc > 3:166124/1‑135 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGCAGGCG‑ATGTCGAAGACCCCCCAGCATTCGCggctcatc > 8:257930/1‑129 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCATTCGCGGGGAAAc > 3:345586/1‑135 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTATCGCGCATGCG‑ATGTCGCATACACCGCCGCATTCGCGGGTAAAc > 1:344852/1‑135 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGGTTTCTCGCGAATGCG‑ATGTCGGATACCCCGCCGCCTTAGCCGGTaaaa > 5:14376/1‑134 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATAAGAACACCGGGG‑TTTTTTTTGCCCGGAATTCGCCTGGAGCGGGTTTTCTCGCGACAGCG‑ATGTAGAATACACCGCCGCATTCGCGGGTaacc > 2:96923/1‑133 (MQ=255) cGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCATTCGCGGGTAAACCCgg < 6:159824/134‑1 (MQ=255) cGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAgg > 1:125125/1‑135 (MQ=255) cGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgc < 7:296171/136‑1 (MQ=255) ggACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgc < 2:125125/135‑1 (MQ=255) ggACTGGGACCCCGAACTTCCCCCTCCCCATACGAAGGCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgc < 3:308763/135‑1 (MQ=255) aCTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgcg > 6:12152/1‑134 (MQ=255) ccccGAACTTCCCCCTCACCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCaaaacc > 1:305625/1‑131 (MQ=255) aCTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCCCTAAACGa > 7:344625/1‑136 (MQ=255) aCTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGa > 8:186612/1‑136 (MQ=255) aCTTCCCCCTCCCCATAAGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCCGGCGCAAACCACTACACg > 2:278206/1‑135 (MQ=39) ccTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt < 6:263386/66‑1 (MQ=255) ccTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt > 5:263386/1‑66 (MQ=255) ccATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCt > 3:179694/1‑135 (MQ=255) gAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGt > 4:25277/1‑135 (MQ=255) aaGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGtt > 1:259610/1‑135 (MQ=255) aaGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGCTCAGGCGCAAACCACTAAACGACAGGCTTGTCTTCCAGtt > 1:253814/1‑135 (MQ=255) tCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTtcca > 2:64939/1‑135 (MQ=255) cGGGGTTTTTTTTT‑CCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGAATTCGCGGGTAAACACGGGTCAGGCGCAAACCACTAAACGCCCGGCTTGTCTTCAAGTtccag > 4:24345/1‑133 (MQ=255) ggggTTTTTTTTTGCGGTGATTTTGCGGGTAGCGGGTTTGCTCG‑GTATGGGTATGTCTAATACACCGCCGCATTCGCGGGTAAACGCGGGTCAGGCGAAAACCACTAAACGACCGGCTTGTCTTCCAGTtccatc < 4:142214/135‑1 (MQ=255) cggggTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTtccatcc > 7:222720/2‑136 (MQ=255) cggggTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCAATCTCGGGTAAACCCCGGTCAGGCGCAAACCACTAAACGACCCGCTTGTCTTCCAGTtccat > 4:43994/2‑134 (MQ=255) | GGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCG‑ATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTTCCATCC > ACB122/120857‑121106 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |