Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 3,857,773 Δ1 bp 6.9% coding (3051/3282 nt) rcsC_7 → Sensor histidine kinase RcsC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,857,7730A. 6.9% 193.8 / 7.8 58coding (3051/3282 nt)rcsC_7Sensor histidine kinase RcsC
Reads supporting (aligned to +/- strand):  ref base A (23/31);  new base . (2/2);  total (25/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

AGCCTTTCCATAGTGGGTTCGACCTGGCCGAACTGCGTCACCTGACCCAGGACGACGAACAGTTGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGTGCGCACTGTGGTCAAAGACTGTGAAGCTATCGAGCACGCCCACGCAAAAGGGCTGCCGACCGTC  >  ACB122/3857640‑3857908
                                                                                                                                     |                                                                                                                                       
agacTTTCCATATTTGGTTCGACCTGGCCGAACTGCGTCGACTGACACAGGACGACGAACAGTTGACTCGTCATTTGCTGGCGCAATTGTCTATAAGCGTCGGTGAAGATCTGGGCGCCCTGCTTGCCTTGTCAAc                                                                                                                                       <  8:326788/133‑1 (MQ=255)
 gCCTTTCCTTTGTGGGTTCGTCCTGGCCGAACTGCGTCACGTGACCCAGGACGACGAACAGTTTACGCTGATTTTGCTGGAGCATTTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGGGTGCCTTGTCAAc                                                                                                                                       <  2:348468/135‑1 (MQ=255)
 gCCTTTCCATAGTGGGTTCGACCTGGCCGAACTGCGTCACCTGACCCAGGACGACGAACAGTTGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAAc                                                                                                                                       <  1:72958/135‑1 (MQ=255)
        cATAGTGGGTTCGACCTGGCCGAACTGCGTCACCTGACCCAGGACGACGAACAGTTGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGc                                                                                                                                 <  1:22800/134‑1 (MQ=255)
        cATAGGGGGTGCGCCCTGGCCGAACTGCGCCACCTGACCCAGCACGACGAACAGTTGACGCGGCATTTGCTGGACAAATTGGCGATACGCGTCGGTGAAGAGCGGGCCGCCCTGGGGGCGTTGTCAACCCACGc                                                                                                                                 <  2:190773/134‑1 (MQ=255)
         aTAGTGGGTTCGCCCTGGCCGAACTGCGTCACCTGACCCAGGACGCCGAACAGTAGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGCTCTGGCCGCCCTGCGTGCATAGTCAACAGACGcc                                                                                                                                >  5:248332/1‑134 (MQ=255)
              gggTTCGACCTGGCCGAACTGCGTCACGTGACCCAGGACGACGAACAGTTGACGCGGCATTTGCGGGAGCATTTGTCGATAAGCGTCGGTGAATAGCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCtgatg                                                                                                                         <  8:179297/136‑1 (MQ=255)
              gggTTCGACCTGGCCGAACTGCGTCACCTGACCCAGGACGACGAACAGTTGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCtgatg                                                                                                                         <  8:89095/136‑1 (MQ=255)
               ggTTCGACCTGGCCGAACTGCGTCACCTGACCCAGGACGACGAACAGTTGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCtgatga                                                                                                                        <  8:139787/136‑1 (MQ=255)
               ggTTCGACCTGGCCGAACTGCGTCACCTGACCCAGGACGACGAACAGTTGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCtgatg                                                                                                                         >  8:195668/1‑135 (MQ=255)
                  tCGACCTGGCCGAACTGCGTCACCTGACCCAGGACGACGAACAGTTGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGaaa                                                                                                                      >  4:136495/1‑135 (MQ=255)
                  tCGACCTGGCCGAACTGCGTCACCTGACCCAGGACGACGAACAGTTGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTC‑ACAGACGCCCCTGATGAAAc                                                                                                                     >  6:26904/1‑135 (MQ=255)
                  tCGACCTGGCCGAACTGCGTCACCTGACCCAGGACGACGAACAGTTGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGGCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTC‑ACAGACGCCCCTGATGAAAc                                                                                                                     >  6:21721/1‑135 (MQ=255)
                    gACCTGGCCGAACTGCGTCACCTGACCCAGGACGACGAACAGTTGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAAcc                                                                                                                    >  4:93666/1‑135 (MQ=255)
                     aCCTGGCCGAACTGCGTCACCTGACCCAGGACGACGATCAGTTGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGt                                                                                                                  >  8:342057/1‑136 (MQ=255)
                     aCCTGGCCGAACTGCGTCACCTGACCCAGGACGACGAACAGTTGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCg                                                                                                                   >  4:122467/1‑135 (MQ=255)
                     aCCTGGCCGAACTGCGTCACCTGACCCAGGACGACGAACAGTTGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGt                                                                                                                  <  7:195668/136‑1 (MQ=255)
                             gAACTGCGTCACCTGACCCAGGACGACGAACAGTTGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTgcgcgc                                                                                                            >  4:15999/1‑134 (MQ=255)
                             gAACTGCGTCACCTGACCCAGGACGACGAACAGTTGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTgc                                                                                                                <  3:381642/130‑1 (MQ=255)
                             gAACTGCGTCACCTGACCCAGGACGACGAACAGTTGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTgc                                                                                                                >  4:381642/1‑130 (MQ=255)
                                    gTCACCTGACCCAGGACGACGAACAGTTGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGcc                                                                                                     <  5:332651/134‑1 (MQ=255)
                                    gTCACCTGACCCAGGACGACGAACAGTTGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGcc                                                                                                     <  3:93666/134‑1 (MQ=255)
                                        ccTGACCCAGGACGACGAACAGTTGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCg                                                                                                >  2:287069/1‑135 (MQ=255)
                                                     gactaACAGTTGACGCGGCATTTTCTTGAGCAATTGTCGATAAGCGGCGGTGAAGATCTGGCCGCCCTGCGGGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGgc                                                                                   <  6:354065/135‑1 (MQ=255)
                                                     gacgaACAGTTGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGgc                                                                                   >  4:311104/1‑135 (MQ=255)
                                                     gacgaACAGTAGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTCCGTGCCTTCTCAAAAGACGCCCCTGATGAAACCGTGCGCGCCCTAGCCCACCGCCTCAACGGTGgcg                                                                                  >  8:90502/1‑136 (MQ=255)
                                                          aCAGTTGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCa                                                                               >  6:180477/1‑134 (MQ=255)
                                                          aCAGTTGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCa                                                                               >  3:111023/1‑134 (MQ=255)
                                                             gTTGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCTGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATg                                                                          <  8:330693/136‑1 (MQ=255)
                                                              ttGCCGCGGCATTTGCTGTAGCAATTGTCTATACGCGGCGGTGAAGATCTGGCCGCCCGGCGTGCAGTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATg                                                                          <  5:232384/135‑1 (MQ=255)
                                                              ttGACGCGGCATTGGCTGGAGCATTTGTCGATACGCGTCGGTGAAGATCTGGCCGCCCTGCGGGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATg                                                                          <  6:56360/135‑1 (MQ=255)
                                                                   gcgGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCtgatga                                                                                                                        >  7:215418/1‑84 (MQ=255)
                                                                   gcgGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCtgatga                                                                                                                        <  8:215418/84‑1 (MQ=255)
                                                                   gcgGCATTTGCTGGAGCAATGGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAg                                                                     <  6:114277/135‑1 (MQ=255)
                                                                    cgGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAggg                                                                   >  7:292174/1‑136 (MQ=255)
                                                                      gCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGt                                                                  <  3:311104/135‑1 (MQ=255)
                                                                          ttGCTGGAGCAATTGTCGATAAGCGTCGTTGAAGATCTGGCCGCCCTGCGTGCCTTGTC‑ACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGTGCGCa                                                             <  5:21721/135‑1 (MQ=255)
                                                                          ttGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTC‑ACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGTGCGCa                                                             <  5:26904/135‑1 (MQ=255)
                                                                           tGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGTGCGCa                                                             <  8:32332/135‑1 (MQ=255)
                                                                            gCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGTGCGCa                                                             <  6:268776/134‑1 (MQ=255)
                                                                            gCTGCAGCATTTGTCGATAAGCGTCGGTGAAGAGCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGAGGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGTGCGCa                                                             <  2:211484/134‑1 (MQ=255)
                                                                                  gCAATTGTCGATAAGGGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGTGTGGCGCCAAAATGATCAGGGTGCGCACTGTGGt                                                      <  6:248332/135‑1 (MQ=255)
                                                                                   cAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGTGCGCACTGTGGTc                                                     <  6:163882/135‑1 (MQ=255)
                                                                                   cAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGTGCGCACTGTGGTc                                                     <  3:179874/135‑1 (MQ=255)
                                                                                    aaTTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTAGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGTGCGCACTGTGGTc                                                     >  4:360890/1‑134 (MQ=255)
                                                                                         tCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGTGCGCACTGTGGTCAAAGa                                                <  6:130373/134‑1 (MQ=255)
                                                                                          cGATACGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGTGCGCACTGTGGTCAAAGACt                                              <  3:55659/135‑1 (MQ=255)
                                                                                              aaGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGTGCGCACTGTGGTCAAAGACtgt                                            >  5:37724/1‑133 (MQ=255)
                                                                                                 cGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGTGCGCACTGTGGTCAAAGACTGTGAAg                                        >  1:187123/1‑134 (MQ=255)
                                                                                                             gcTGGCCGCCCTGCGTGCCTTGTCAACCGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGTGCGCACTGTGGTCAAAGACTGTGAAGCTATCGAGCACGc                           <  5:273758/134‑1 (MQ=255)
                                                                                                                    gccCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGTGCGCa                                                             >  2:308300/1‑94 (MQ=255)
                                                                                                                    gccCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGTGCGCa                                                             <  1:308300/94‑1 (MQ=255)
                                                                                                                    gccCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGTGCGCACTGTGGTCAAAGACTGTGAAGCTATCGAGCACGCCCACGc                     >  2:296903/1‑134 (MQ=255)
                                                                                                                    gccCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGTGCGCACTGTGGTCAAAGACTGTGAAGCTATCGAGCACGCCCACGc                     >  5:94933/1‑134 (MQ=255)
                                                                                                                    gccCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGTGCGCACTGTGGTCAAAGACTGTGAAGCTATCGAGCACGCCCACGCa                    >  8:275151/1‑135 (MQ=255)
                                                                                                                              ccTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGTGCGCACTGTGGTCAAAGACTGTGAAGCTATCGAGCACGCCCACGCAAAAGGGCTGc          <  6:37724/135‑1 (MQ=255)
                                                                                                                              ccTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGTGCGCACTGTGGTCAAAGACTGTGAAGCTATCGAGCACGCCCACGCAAAAGGGCTGc          >  6:337745/1‑135 (MQ=255)
                                                                                                                               attGTCAACCGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGTGCGCACTGTGGTCAAAGACTGTGAAGCTATCGAGCACGCCCACGCAAAAGGGCTGc          <  3:393307/133‑1 (MQ=255)
                                                                                                                                     aaCAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGTGCGCACTGTGGTCAAAGACTGTGAAGCTATCGAGCACGCCCACGCAAAAGGGCTGCCGACCGTc  >  7:295747/1‑136 (MQ=255)
                                                                                                                                     |                                                                                                                                       
AGCCTTTCCATAGTGGGTTCGACCTGGCCGAACTGCGTCACCTGACCCAGGACGACGAACAGTTGACGCGGCATTTGCTGGAGCAATTGTCGATAAGCGTCGGTGAAGATCTGGCCGCCCTGCGTGCCTTGTCAACAGACGCCCCTGATGAAACCGTGCGCGCCCTTGCCCACCGCATCAAGGGTGGCGCCAAAATGATCAGGGTGCGCACTGTGGTCAAAGACTGTGAAGCTATCGAGCACGCCCACGCAAAAGGGCTGCCGACCGTC  >  ACB122/3857640‑3857908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑