Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 478,881 A→C 22.2% V35G (GTC→GGC)  apaH_1 ← Bis(5'‑nucleosyl)‑tetraphosphatase, symmetrical

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122478,8810AC22.2% 49.4 / 2.2 39V35G (GTC→GGC) apaH_1Bis(5'‑nucleosyl)‑tetraphosphatase, symmetrical
Reads supporting (aligned to +/- strand):  ref base A (14/14);  new base C (6/2);  total (23/16)
Fisher's exact test for biased strand distribution p-value = 2.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGTTGTGCCAGGCGGCCAGCAGGTGCAGGTCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGAC‑GGC‑GGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCACTGCCAGCC  >  ACB122/478755‑479012
                                                                                                                              |                                                                                                                                     
aCGTTGTGCCTGGCGGCCAGCTGGTGCAGGTCATGGGTGCCCAGAACGCAGACCAGTTAATCAGGGATCGAGTAGAGGAAGCGCTGGGTTTCCAGTGACTGGGGGCCGCGGTTGACCAGGTCGCCGACCAGCcaa                                                                                                                               <  4:17358/135‑2 (MQ=255)
                ccAGCAGGTGCAGGTCATGGTTGCCCCGCACGCAGACCAGTGAATGACGGAGCGAGTACAGGAAGCGCAGGGTTTCCAGTGACGCGGGGCCGCGGTTGACCAGGGCGCCGACCAGCCACAGGCGGGCGAc‑ggc‑gg                                                                                                             <  3:47864/135‑1 (MQ=255)
                   gcaggtgcaggtCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGCCCAGGTCGCGGGCCCGCCCCCCGCGGGGGCC‑GGC‑ggggg                                                                                                          >  1:398616/1‑134 (MQ=255)
                    tgcgtgcaggtCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGGTTCCAGTGACTCGGGGCCGCGGTTGCCCAGGTCGCCGCCCAGCCCCAGGCGGGCGAC‑GGC‑gggggt                                                                                                         >  4:237717/4‑133 (MQ=255)
                                    ttGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCAGCCACAGGCGGTCGAC‑GGC‑GGGGTTGAAACGGACGCGATCg                                                                                         >  6:160868/1‑135 (MQ=255)
                                          aGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGGCCACCCACAGGCGGGCGCC‑GGC‑GCGGTGTCAACGGACGCGCTCGACCAgcg                                                                                  >  7:92654/1‑134 (MQ=255)
                                          aGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGAc‑ggc‑gg                                                                                                             >  1:8394/1‑109 (MQ=255)
                                          aGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGAc‑ggc‑gg                                                                                                             <  2:8394/109‑1 (MQ=255)
                                               gCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCCCAGGCGGTCGAC‑GGC‑GGGGGTGAAACTGACGCGATCGAGCAGGCAttt                                                                              >  7:266925/1‑135 (MQ=255)
                                               gCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGAC‑GGC‑GGGGTTGAAACTGACGCGATCGAGCAGGCAtt                                                                               >  5:108486/1‑134 (MQ=255)
                                                     cAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCCCCCACCCGCGCTCGCCGGGC‑GGGGGT‑CAACAGCCCCGCGCGACCAGGCAATTAAGCGGc                                                                       >  7:337929/1‑136 (MQ=255)
                                                                ggATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCCCCCACACGCGGTGGCC‑CGCGGGGGGTCAAAC‑GACGCCCACGAGCACGCCTTTGAGCGGCTGCAcgccgc                                                             >  2:219648/1‑135 (MQ=255)
                                                                     gAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGGCCAGCACCAGGCGGTGGAC‑CGC‑GGGGGTGAAACAGACGCGAACGAGCAAGCCTTTGAGCGGCAGCAGGCAGCCCAGcc                                                       >  7:153524/1‑135 (MQ=255)
                                                                     gAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGCC‑GGC‑GGGGTTGAAAATGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCt                                                          >  1:210482/1‑133 (MQ=255)
                                                                                 cgcCGGGTTTCCCGAGACTCGGGGCCGCGTTGGACCTGGTCGCCGACCAGCAACAGGCGGTCGAC‑GGC‑GGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACgg                                           <  8:90851/136‑1 (MQ=255)
                                                                                  gctgggTGTCCCGTGACTCGGGGCCGCGGTTCACCCGGTCGCCGACCAGCCACTGGCGGGCGAC‑GGC‑GGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACgg                                           <  3:199335/132‑1 (MQ=255)
                                                                                   cAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGGC‑GGC‑GGGGTTGAAACTGACGCGATCGCGCAGGGATTTGAGCGGCTGCAGGCAGCCCTGCCGGTCACCGCCGGCg                                         >  7:369607/1‑136 (MQ=255)
                                                                                   cAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGAC‑GGC‑GGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGACCTGCAGGTCACCGACGGCg                                         >  8:288988/1‑136 (MQ=255)
                                                                                   cAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCCCCCACCCCCAGGCGGGCGCC‑GGC‑CGGGTTGAAACTGACCCGATCGAGCAGGCATTTTAGCGGCAGCAGGCCGCCCTGCACGTCCCCCACCGc                                          >  5:322237/1‑135 (MQ=255)
                                                                                    aGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGCCCCGCCACCGGCGGTCGCC‑GGC‑GGGGGTGCAACTGACGCGATCGAGCAGGCAGTTGAGCGGCTGCCGGCAGCCCGGCAGGTCCCCGCCGGc                                          >  2:237996/1‑134 (MQ=255)
                                                                                    aGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGAC‑GGC‑GGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCg                                         >  2:48886/1‑135 (MQ=255)
                                                                                     gggTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGAC‑GGC‑GGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGc                                          >  5:156243/1‑133 (MQ=255)
                                                                                      ggTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGGCGCCGACCAGCCCCAGGCGGTCGAC‑GGC‑GGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAg                                      <  8:173002/136‑1 (MQ=255)
                                                                                       ttttCCAGTGACGCGGGGCCGCGGTGGACCTGGGCGCCTCACAGCCACCGGGGTTCCAC‑GGC‑GGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAg                                      <  6:8/134‑1 (MQ=255)
                                                                                       gtctCCAGTGACGCGGGGCCGCGGTTGACCAGGCCCCCGACCAGCCACCGGCGGGCGAC‑GGC‑GGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAg                                      <  4:324844/132‑1 (MQ=255)
                                                                                        tttCCAGGGACGCGGGGCCGCGGGTGACCCGGGCGCCGACCCGCCACAGGCGGTCGAC‑GGC‑GGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGt                                    <  8:255477/136‑1 (MQ=255)
                                                                                         ttCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGAC‑GGC‑GGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCCCCGACGGCGTAGGtt                                   >  7:90851/1‑135 (MQ=255)
                                                                                           cccgggACGCGGGGCCGCGGGTGCCCAGGGCGCCGACCAGCCACAGGCGGGCGAC‑GGC‑GGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGc                                 <  7:239859/131‑1 (MQ=255)
                                                                                            cggTGCCTCGGGGCGGGGGTGGACCCGGGCGCGGACCCGCCACGGGGGGGCGAC‑GGC‑GGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGc                                 <  6:322237/133‑1 (MQ=255)
                                                                                                  cgcGGGGCCGCGTGTGACCAGGGCGCCGACCAGCCACAGGCGGTCGAC‑GGC‑GGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGt                          <  8:80168/134‑1 (MQ=255)
                                                                                                   tgggggCGGCGGTGGACCCGGCCGCCGCCCCCCCCCGGGGGGGGCAC‑GGC‑GGGGTTGAAACTGACGCGATCGCGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGt                          <  1:219648/133‑1 (MQ=255)
                                                                                                   tCGGGGCCGCGGTTGACCAGGTCGCCGCCCAGCCACACGCGGTCGCC‑GGC‑GGGGTTGAAACTGACGCGATCGAGCACGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCCCCGACGGCGTAGGTGGCCCTCAGTg                         >  7:160000/1‑136 (MQ=255)
                                                                                                   tCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGAC‑GGC‑GGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGt                          >  4:272496/1‑135 (MQ=255)
                                                                                                                tGACCAGGTCGCAGACCAGCCACAGGCGGTCGAC‑GGC‑GGGGTTTAAACTGACGCGATCGAGCAGGCATTTGAGCCGCTGCAGGCCGCCCAGCCGGTCACCGACGGCGTAGGTGGCCATCAGTTCAGCGCTCCcgg             >  6:125752/1‑135 (MQ=255)
                                                                                                                  aCCAGGGCGCCCACCAGCCACAGGCGGTCGAC‑GGC‑GGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCAc          <  8:26064/136‑1 (MQ=255)
                                                                                                                   gccgggcgccgcCCCGCCACCGGCGGGCGAC‑GGC‑GGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCAc          <  5:93116/129‑1 (MQ=255)
                                                                                                                   ccAGGTCGCCGACCAGCCACAGGCGGTCGAC‑GGC‑GGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCa           <  2:79692/134‑1 (MQ=255)
                                                                                                                     aGGTCGCCGACCAACCACAGGCGGTCGAC‑GGC‑GGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCACTg        >  5:186632/1‑135 (MQ=255)
                                                                                                                       ggcGCCGACCAGCCACAGGCGGTCGAC‑GGC‑GGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCACTGcc      <  4:175485/133‑1 (MQ=255)
                                                                                                                            cGACCAGCCACAGGCGGTCGAC‑GGC‑GGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCACTGCCAGcc  >  2:101039/1‑134 (MQ=255)
                                                                                                                              |                                                                                                                                     
ACGTTGTGCCAGGCGGCCAGCAGGTGCAGGTCATGGTTGCCCAGCACGCAGACCAGTGAATGACGGATCGAGTACAGGAAGCGCAGGGTTTCCAGTGACTCGGGGCCGCGGTTGACCAGGTCGCCGACCAGCCACAGGCGGTCGAC‑GGC‑GGGGTTGAAACTGACGCGATCGAGCAGGCATTTGAGCGGCTGCAGGCAGCCCTGCAGGTCACCGACGGCGTAGGTGGCCATCAGTGCAGCGCTCCGGGCACTGCCAGCC  >  ACB122/478755‑479012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑