Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,423,488 A→C 13.3% S133A (TCG→GCG)  CJ019_05102 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,423,4880AC13.3% 149.5 / 2.6 75S133A (TCG→GCG) CJ019_05102hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (45/18);  new base C (9/3);  total (54/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGCAGCACACCACCGGCCACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCACCCGAACCAAAGATGCTGGACAAGAAGCCACCCCCGCCATTGCTCGGC  >  ACB122/5423356‑5423618
                                                                                                                                    |                                                                                                                                   
ccAGCAGCACACCACCGGCCACACCGGCGGCGGTCTGCCTGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGCAACCAGGTGCCGACg                                                                                                                                   >  2:132787/1‑135 (MQ=255)
ccAGCAGCACACCACCGGCCACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCCTATCCAGGCTGCGGCGCCTGCGGCTGGGCGAAACCCGGTGCCGACg                                                                                                                                   >  4:230631/1‑135 (MQ=255)
ccAGCAGCACACCACCGGCCACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGCGGCTGGGCGAAACCCGGTGCCGACg                                                                                                                                   >  4:256963/1‑135 (MQ=255)
ccAGCAGCACACCACCGGCCACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGCGGCTGGGCGAAACCAGGTGCCGACgg                                                                                                                                  >  8:164553/1‑136 (MQ=255)
ccAGCAGCACACCACCGGCCACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGccgccg                                                                                                                                   >  5:184844/1‑135 (MQ=255)
ccAGCAGCACACCACCGGCCACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACgg                                                                                                                                  >  8:92939/1‑136 (MQ=255)
ccAGCAGCACACCACCGGCCACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACg                                                                                                                                   >  5:297587/1‑135 (MQ=255)
ccAGCAGCACACCACCGGCCACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACg                                                                                                                                   >  1:30147/1‑135 (MQ=255)
ccAGCAGCACACCACCGGCCACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACg                                                                                                                                   >  6:375093/1‑135 (MQ=255)
ccAGCAGCACACCACCGGCCACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCGGGGCGAAACCCGGTGCCGACg                                                                                                                                   >  3:244906/1‑135 (MQ=255)
ccAGCAGCACACCACCGGCCACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCAGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACgg                                                                                                                                  >  8:132510/1‑136 (MQ=255)
 cagcagCACACCACCGGCCACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCCTAGCCCGGCTGCGGCGCCTGCGGCTGGGCGCACCCCGGTGCCGACgg                                                                                                                                  >  3:19676/1‑135 (MQ=255)
 cagcagCACACCACCGGCCACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGCCgg                                                                                                                                  >  5:274109/1‑135 (MQ=255)
 cagcagCACACCACCGGCCACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGCCgg                                                                                                                                  >  3:36950/1‑135 (MQ=255)
 cagcagCACACCACCGGCCACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACgg                                                                                                                                  >  3:287116/1‑135 (MQ=255)
 cagcagCACACCACCGGCCACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGc                                                                                                                                 >  8:48075/1‑136 (MQ=255)
      gcaCACCACCGGCCACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGGCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAACACAGGTGCCGCCGGCT‑cgc                                                                                                                            >  3:285635/1‑135 (MQ=255)
      gcaCACCACCGGCCACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑cgc                                                                                                                            >  5:263094/1‑135 (MQ=255)
       cacaCCACCGGCCACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGCCGGCT‑cgcg                                                                                                                           >  1:129/1‑135 (MQ=255)
       cacaCCACCGGCCACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑cgcg                                                                                                                           >  6:139632/1‑135 (MQ=255)
       cacaCCACCGGCCACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑cgcg                                                                                                                           >  4:330099/1‑135 (MQ=255)
        acacCACCGGCCACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCAGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCgcc                                                                                                                         <  7:132510/136‑1 (MQ=255)
           cccccGGCCACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCCTAGCCAGGCTGCGGCGCCTGAGGCGGGGCGAACCCAGGTGCCGCCGGCT‑CCCgccag                                                                                                                       >  1:146207/4‑135 (MQ=255)
              ccGGCCCCCCCGGGGGCGGTCTGCATGGCCCCGCCACGGAAGCTGCGGGCCCCGCTGCGCGCCGGCGCGGCGCCATCGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGGGCCGACGGCT‑CGCgccagcca                                                                                                                    <  1:397361/135‑1 (MQ=255)
               cttccACACCGGCGGAGGTCTGCATGGCCAAGCCAAGGAAGCTGCTGGCCCCGCTGCGCGAAGGCGCGGCGCCCTAGCCAGGCTGCGTCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGAT‑CGCGCCAGccac                                                                                                                   >  4:170915/4‑135 (MQ=255)
                 gCCACCCCGGGGGCGGGCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGccacca                                                                                                                 <  4:19676/135‑1 (MQ=255)
                 gCCACACCGGCGGCGGGCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCCGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGACACCAGGTGCCGACGGCT‑CGCGCCCGccacca                                                                                                                 >  1:236127/1‑135 (MQ=255)
                  ccACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCaccacc                                                                                                               >  7:76440/1‑136 (MQ=255)
                    acacCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGCAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCgg                                                                                                             >  7:381988/1‑136 (MQ=255)
                    acacCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCg                                                                                                              <  6:297587/135‑1 (MQ=255)
                         ggcggcggTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCCGCCCCCACCGGCCGc                                                                                                         >  1:365292/1‑135 (MQ=255)
                           cggcggTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGCGGCTGGGCGAAACCCGGTGCCGCCGGCT‑CGCGCCAGCCACCACCGGACGCt                                                                                                        >  3:163280/1‑134 (MQ=255)
                               ggTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAgg                                                                                                   <  4:122392/135‑1 (MQ=255)
                                   tGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCCGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCCGCCCCCACCGGACGCTACAGGGGgcg                                                                                              >  7:243892/1‑136 (MQ=255)
                                   tGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGCGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCCCCGGACGCGACCGGGGgc                                                                                               >  3:221260/1‑135 (MQ=255)
                                          ccccGCCAAGGAAGCGGCGGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCGGGGGCGCCGGAGGCTGGGCGAAACCCGGTGCCGACGGCG‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAg                                                                                        <  1:49597/135‑1 (MQ=255)
                                             cGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGAc                                                                                     >  1:51921/1‑135 (MQ=255)
                                             cGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACg                                                                                    >  8:94124/1‑136 (MQ=255)
                                              gCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGCCGGCT‑CGCGCCAGCCCCCACCGGACGCTACAGGGGGCGCCGAAGGACg                                                                                    >  5:129490/1‑135 (MQ=255)
                                                cAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCCTAGCCAGGCTGCGGCGCCTGAGGCGGGGCGAAACCAGGTGCCGCCGGCT‑CGCGCCAGCCCCCCCCGGCCGCTACAGGGGGCGCCGCAGGACGcgg                                                                                 >  7:143458/1‑134 (MQ=255)
                                                cAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCACGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCt                                                                                  >  5:134451/1‑135 (MQ=255)
                                                  aGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCTGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGCCGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCtgtg                                                                               >  8:366338/1‑136 (MQ=255)
                                                    gAAGCTGCTGGGCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTTTgg                                                                              <  5:190513/135‑1 (MQ=255)
                                                            gggCCCCGCGGCGCGCTGGCGCGGCGCCATAGCCCGGCGGCGGCGCCGGAGGCGGGGCGAAACCCGGGGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCAccg                                                                      <  4:221260/134‑1 (MQ=255)
                                                                 cggCTGCGCGCCGGCGCGGCGCCATAGCCTGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGCCGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCggg                                                                 <  3:246453/133‑1 (MQ=255)
                                                                      gcgcgcAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAACCCAGGTGCCGACGGCT‑CGCGCCAGCCACCCCCGGCCGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGc                                                            >  4:106450/1‑135 (MQ=255)
                                                                      gcgcgcAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCAc                                                                        >  2:390592/1‑123 (MQ=255)
                                                                      gcgcgcAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCAc                                                                        <  1:390592/123‑1 (MQ=255)
                                                                      gcgcgcAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCg                                                           >  7:114066/1‑136 (MQ=255)
                                                                        gcgcAGGCGCGGCGCCATAGCCCGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCCCCGGACGCGACAGGGGGCGCCGACGGACGCTGTGGCTGCACCGCCGGGGCTGCGtt                                                         >  7:70639/1‑136 (MQ=255)
                                                                        gcgcAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGtt                                                         <  7:94124/136‑1 (MQ=255)
                                                                                    cgcCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAACCCAGGTGCCGACGGCT‑CGCGCCAGCCCCCACCGGCCGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCCCCCg                                              >  3:262855/1‑135 (MQ=255)
                                                                                       cATCGCCCGGCGGCGGCGCCGGTGGCTGGGCTAAACCCGGGGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCACCCGAAc                                           <  5:381964/135‑1 (MQ=255)
                                                                                       cATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCACCCGAAc                                           <  2:104236/135‑1 (MQ=255)
                                                                                         tAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑AGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCACCCGAAcc                                          >  3:154772/1‑134 (MQ=255)
                                                                                                 cTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCACCCGAACCAAAGATGCTg                                >  7:149455/1‑136 (MQ=255)
                                                                                                  tGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCACCCGAACCAAAGATGCTgg                               <  7:92939/136‑1 (MQ=255)
                                                                                                   gcggcgCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCCCCACCGGACGCTACACTCGGCGCCGAAGGACGCTGAGGCTGCACCGCCGGGCCTGCGTTGCGCCCACCCGAACCAAAGAAGCTgg                               >  5:106196/1‑135 (MQ=255)
                                                                                                   gcggcgCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGCCGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCACCCGAACCAAAGCTGCTgg                               >  1:3514/1‑135 (MQ=255)
                                                                                                   gcggcgCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCACCCGAACCAAAGATGCTGGa                              >  7:33852/1‑136 (MQ=255)
                                                                                                         ccTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGCCGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCCCCCGAACCAAAGATGCTGGACaa                           >  6:144989/1‑133 (MQ=255)
                                                                                                         ccTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGCCGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCACCCGAACCAAAGATGCTGGACaag                          >  8:4644/1‑134 (MQ=255)
                                                                                                         ccTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCACCCGAACCAAAGATGCTGGACaa                           >  6:144992/1‑133 (MQ=255)
                                                                                                             aGGCGGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCACCCGAACCAAAGATGCTGGACAAGAAGcc                     <  4:244906/135‑1 (MQ=255)
                                                                                                                     cGAAACCAGGGGCCGACGGCTCCGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCACCCGAACCAAAGATGCTGGACAAGAAGCCACCCCCg              <  1:132787/135‑1 (MQ=255)
                                                                                                                      gAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCACCCGAACCAAAGATGCTGGACAAGAAGCCAc                   >  4:232418/1‑128 (MQ=255)
                                                                                                                      gAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCACCCGAACCAAAGATGCTGGACAAGAAGCCAc                   <  3:232418/128‑1 (MQ=255)
                                                                                                                      gAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCACCCGAACCAAAGATGCTGGACAAGAAGCCACCCCCGcc            >  2:25999/1‑135 (MQ=255)
                                                                                                                      gAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCACCCGAACCAAAGATGCTGGACAAGAAGCCACCCCCGCCa           >  7:371311/1‑136 (MQ=255)
                                                                                                                      gAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCACCCGAACCAAAGATGCTGGACAAGAAGCCACCCCCGCCa           >  7:36758/1‑136 (MQ=255)
                                                                                                                      gAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCACCCGAACCAAAGATGCTGGACAAGAAGCCACCCCCGCCa           >  7:260404/1‑136 (MQ=255)
                                                                                                                        aaCCCGGGGCCGCCGGCG‑CGCGCCAGCCACCACCGGCCGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCACCCGAACCAAAGATGCTGGACAAGAAGCCACCCCCGCCAtt         <  7:164553/136‑1 (MQ=255)
                                                                                                                           cAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCACCCGAACCAAAGATGCTGGACAAGAAGCCACCCCCGCCATTGc       >  5:220783/1‑135 (MQ=255)
                                                                                                                              gTGCCGACGGGT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCGGCACCGCCGGGGCTGCGTTGCGCCCACCCGAACCAAAGATGCTGGACAAGAAGCCACCCCCGCCATTGCTCg    <  6:369418/135‑1 (MQ=255)
                                                                                                                               tGCCGCCGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCACCCGAACCAAAGATGCTGGACAAGAAGCCACCCCCGCCATTGCTCg    <  4:139290/134‑1 (MQ=255)
                                                                                                                                gCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCACCCGAACCAAAGATGCTGGACAAGAAGCCACCCCCGCCATTGCTCGgc  <  3:94736/135‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                   
CCAGCAGCACACCACCGGCCACACCGGCGGCGGTCTGCATGGCCCCGCCAAGGAAGCTGCTGGCCCCGCTGCGCGCAGGCGCGGCGCCATAGCCAGGCTGCGGCGCCTGAGGCTGGGCGAAACCAGGTGCCGACGGCT‑CGCGCCAGCCACCACCGGACGCTACAGGGGGCGCCGAAGGACGCTGTGGCTGCACCGCCGGGGCTGCGTTGCGCCCACCCGAACCAAAGATGCTGGACAAGAAGCCACCCCCGCCATTGCTCGGC  >  ACB122/5423356‑5423618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑