Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,471,653 G→A 5.3% intergenic (‑132/‑12) cidA_2 ← / → CJ019_05151 Holin‑like protein CidA/putative enoyl‑CoA hydratase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,471,6530GA 5.3% 251.3 / 3.1 76intergenic (‑132/‑12)cidA_2/CJ019_05151Holin‑like protein CidA/putative enoyl‑CoA hydratase 1
Reads supporting (aligned to +/- strand):  ref base G (36/36);  new base A (2/2);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01

GCATAAGCATCTCCCTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCGGAGTGGTTGAAGATCGACCAGCAGCGCATCAACCTGTTCGCCGAGGCGACCGGCGATTTCCA  >  ACB122/5471518‑5471786
                                                                                                                                       |                                                                                                                                     
gCATAAGCATCTCCCTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCg                                                                                                                                       <  7:386840/136‑1 (MQ=255)
         tctcCCTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTcc                                                                                                                               >  4:371098/1‑135 (MQ=255)
             ccTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCcgc                                                                                                                             <  7:175315/133‑1 (MQ=255)
             ccTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGAt                                                                                                                          <  7:51578/136‑1 (MQ=255)
              ctggggCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGAt                                                                                                                          <  3:217554/132‑1 (MQ=255)
              cTCGGGCCTGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCcgc                                                                                                                             <  3:10413/132‑1 (MQ=255)
              cTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCcgc                                                                                                                             <  1:255218/132‑1 (MQ=255)
              cTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGAt                                                                                                                          >  4:123114/1‑135 (MQ=255)
              cTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGAt                                                                                                                          >  3:310556/1‑135 (MQ=255)
              cTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGAt                                                                                                                          >  4:271828/1‑135 (MQ=255)
              cTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGAt                                                                                                                          >  6:179514/1‑135 (MQ=255)
              cTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGAt                                                                                                                          >  1:146117/1‑135 (MQ=255)
              cTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATg                                                                                                                         >  7:76466/1‑136 (MQ=255)
              cTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATg                                                                                                                         >  7:341191/1‑136 (MQ=255)
              cTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATg                                                                                                                         >  7:38507/1‑136 (MQ=255)
              cTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACACGGAGAGTCCGCGAt                                                                                                                          >  1:253450/1‑135 (MQ=255)
                 gtgCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGccc                                                                                                                      <  8:211080/134‑1 (MQ=255)
                  ggCATGGGCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGc                                                                                                                        <  5:4719/133‑1 (MQ=255)
                   gCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGcccc                                                                                                                     >  6:132409/1‑135 (MQ=255)
                    cATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCcgc                                                                                                                             <  6:377076/126‑1 (MQ=255)
                    cATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCcgc                                                                                                                             >  5:377076/1‑126 (MQ=255)
                       ggCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCTACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGt                                                                                                                 <  6:368667/135‑1 (MQ=255)
                       ggCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGt                                                                                                                 >  6:112204/1‑135 (MQ=255)
                          cgccATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACACCCCGGAGAGTCCGCGATGCCCCATGTACCg                                                                                                             >  8:27151/1‑136 (MQ=255)
                          cgccATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTAcc                                                                                                              >  6:129280/1‑135 (MQ=255)
                              aTTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTa                                                                                                         <  8:341191/136‑1 (MQ=255)
                               ttATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTa                                                                                                         <  5:49055/135‑1 (MQ=255)
                                tataAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGtt                                                                                                          >  2:328086/1‑133 (MQ=255)
                                   aaGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACag                                                                                                      <  1:112335/134‑1 (MQ=255)
                                          cTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTa                                                                                                         <  5:88949/124‑1 (MQ=255)
                                          cTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTa                                                                                                         >  6:88949/1‑124 (MQ=255)
                                             ggCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTc                                                                                               >  4:18780/1‑131 (MQ=255)
                                             ggCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTc                                                                                               <  3:18780/131‑1 (MQ=255)
                                             ggCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCa                                                                                            >  1:248042/1‑134 (MQ=255)
                                             ggCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCa                                                                                            >  6:81978/1‑134 (MQ=255)
                                                   aGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCa                                                                                                                    >  5:113311/1‑104 (MQ=255)
                                                   aGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCa                                                                                                                    <  6:113311/104‑1 (MQ=255)
                                                     acagATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTg                                                                                    <  6:271721/131‑1 (MQ=255)
                                                     aCCGATATTCGCCGAAACGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTg                                                                                    <  2:376094/134‑1 (MQ=255)
                                                     aCCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTg                                                                                    <  1:372703/134‑1 (MQ=255)
                                                     aCCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTg                                                                                    <  1:214152/134‑1 (MQ=255)
                                                     aCCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCAGAGAGTTCGCGATGCCCCATGTACCGGTTACAGAGCttt                                                                                                <  8:45069/122‑1 (MQ=255)
                                                     aCCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCAGAGAGTTCGCGATGCCCCATGTACCGGTTACAGAGCttt                                                                                                <  8:38558/122‑1 (MQ=255)
                                                     aCCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCAGAGAGTTCGCGATGCCCCATGTACCGGTTACAGAGCttt                                                                                                >  7:45069/1‑122 (MQ=255)
                                                     aCCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCAGAGAGTTCGCGATGCCCCATGTACCGGTTACAGAGCttt                                                                                                >  7:38558/1‑122 (MQ=255)
                                                         atatTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAg                                                                               <  3:371098/135‑1 (MQ=255)
                                                          tatTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTg                                                                                    <  4:385123/129‑1 (MQ=255)
                                                          tatTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTg                                                                                    >  3:385123/1‑129 (MQ=255)
                                                            ttCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACag                                                                                                      <  3:168237/109‑1 (MQ=255)
                                                            ttCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACag                                                                                                      >  4:168237/1‑109 (MQ=255)
                                                            ttCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTg                                                                                    <  5:307982/127‑1 (MQ=255)
                                                            ttCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTg                                                                                    >  6:307982/1‑127 (MQ=255)
                                                            ttCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAg                                                                            >  5:224514/1‑135 (MQ=255)
                                                            ttCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAg                                                                            >  2:135436/1‑135 (MQ=255)
                                                            ttCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAg                                                                            >  6:187833/1‑135 (MQ=255)
                                                            ttCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACAAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAg                                                                            >  5:343606/1‑135 (MQ=255)
                                                                  gAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTggg                                                                       >  4:184879/1‑134 (MQ=255)
                                                                   aaaaGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGcc                                                                     <  1:135436/135‑1 (MQ=255)
                                                                   aaaaGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGcc                                                                     <  5:112204/135‑1 (MQ=255)
                                                                   aaaaGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACAAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGcc                                                                     <  4:217717/135‑1 (MQ=255)
                                                                      aGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTgtttcaa                                                                              <  5:45969/123‑7 (MQ=255)
                                                                      aGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTgtttcaa                                                                              >  6:45969/1‑117 (MQ=255)
                                                                        cGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCg                                                                <  3:90212/135‑1 (MQ=255)
                                                                        cGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCg                                                                <  3:297473/135‑1 (MQ=255)
                                                                        cGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCg                                                                <  3:271828/135‑1 (MQ=255)
                                                                        cGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTATCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCg                                                                <  5:177370/135‑1 (MQ=255)
                                                                                   aCTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCGGAGTGGTTGa                                                      <  4:310556/134‑1 (MQ=255)
                                                                                           ggTTCGCCGTGCTTATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGTCAAGGAGCTGGGCCACTCGGAGTGGTTGAAGATCGAcc                                             <  5:129280/135‑1 (MQ=255)
                                                                                            gTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCGGAGTGGTTGAAGATCGACca                                            <  3:115877/135‑1 (MQ=255)
                                                                                            gTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCGGAGTGGTTGAAGATCGACca                                            >  1:251955/1‑135 (MQ=255)
                                                                                                                 cTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCGGAGTGGTTGAAGATCGACCAGCAGCGCATCACCCTGTTCGcc                      >  8:386064/1‑136 (MQ=255)
                                                                                                                  tttCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCGGAGTGGTTGAAGATCGACCAGCAGCGCATCAACCTGTTCGcc                      <  2:146117/135‑1 (MQ=255)
                                                                                                                      gTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCGGAGTGGTTGAAGATCGACCAGCAGCGCATCAACCTGTTCGCCGAgg                  >  1:369586/1‑135 (MQ=255)
                                                                                                                      gTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCGGAGTGGTTGAAGATCGACCAGCAGCGCATCAACCTGTTCGCCGAgg                  >  5:213629/1‑135 (MQ=255)
                                                                                                                       tACCAACTGACAACCCGGAGAGTCCGCGATGCACAAGGTACCGGTTACAGAGCTTTCGCAGTACGTTGCCAAGCAGCTGGGCCACTCGGAGTGGTTGAAGATCGCCCAGCAGCTCATCAACCTCTTCGCCGAgga                 >  1:281615/1‑134 (MQ=255)
                                                                                                                               gACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCGGAGTGGTTGAAGCTCGACCAGCAGCGCATCAACCTGTTAGCCGCTGCGACCGGCg         >  2:237414/1‑135 (MQ=255)
                                                                                                                                      cGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCGGAGTGGTTGAAGATCGACCAGCAGCGCATCAACCTGTTCGCCGAGGCGACCGGCGATTTCCa  <  2:251955/135‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
GCATAAGCATCTCCCTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCGGAGTGGTTGAAGATCGACCAGCAGCGCATCAACCTGTTCGCCGAGGCGACCGGCGATTTCCA  >  ACB122/5471518‑5471786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑