Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,471,660 C→T 5.2% intergenic (‑139/‑5) cidA_2 ← / → CJ019_05151 Holin‑like protein CidA/putative enoyl‑CoA hydratase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,471,6600CT 5.2% 259.0 / 2.9 76intergenic (‑139/‑5)cidA_2/CJ019_05151Holin‑like protein CidA/putative enoyl‑CoA hydratase 1
Reads supporting (aligned to +/- strand):  ref base C (35/37);  new base T (2/2);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

TCTCCCTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCGGAGTGGTTGAAGATCGACCAGCAGCGCATCAACCTGTTCGCCGAGGCGACCGGCGATTTCCAGTTCATCC  >  ACB122/5471527‑5471794
                                                                                                                                     |                                                                                                                                      
tctcCCTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTcc                                                                                                                                       >  4:371098/1‑135 (MQ=255)
    ccTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCcgc                                                                                                                                     <  7:175315/133‑1 (MQ=255)
    ccTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGAt                                                                                                                                  <  7:51578/136‑1 (MQ=255)
     ctggggCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGAt                                                                                                                                  <  3:217554/132‑1 (MQ=255)
     cTCGGGCCTGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCcgc                                                                                                                                     <  3:10413/132‑1 (MQ=255)
     cTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCcgc                                                                                                                                     <  1:255218/132‑1 (MQ=255)
     cTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGAt                                                                                                                                  >  1:146117/1‑135 (MQ=255)
     cTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGAt                                                                                                                                  >  4:271828/1‑135 (MQ=255)
     cTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGAt                                                                                                                                  >  6:179514/1‑135 (MQ=255)
     cTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGAt                                                                                                                                  >  4:123114/1‑135 (MQ=255)
     cTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGAt                                                                                                                                  >  3:310556/1‑135 (MQ=255)
     cTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATg                                                                                                                                 >  7:38507/1‑136 (MQ=255)
     cTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATg                                                                                                                                 >  7:341191/1‑136 (MQ=255)
     cTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATg                                                                                                                                 >  7:76466/1‑136 (MQ=255)
     cTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACACGGAGAGTCCGCGAt                                                                                                                                  >  1:253450/1‑135 (MQ=255)
        gtgCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGccc                                                                                                                              <  8:211080/134‑1 (MQ=255)
         ggCATGGGCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGc                                                                                                                                <  5:4719/133‑1 (MQ=255)
          gCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGcccc                                                                                                                             >  6:132409/1‑135 (MQ=255)
           cATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCcgc                                                                                                                                     <  6:377076/126‑1 (MQ=255)
           cATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCcgc                                                                                                                                     >  5:377076/1‑126 (MQ=255)
              ggCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCTACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGt                                                                                                                         <  6:368667/135‑1 (MQ=255)
              ggCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGt                                                                                                                         >  6:112204/1‑135 (MQ=255)
                 cgccATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACACCCCGGAGAGTCCGCGATGCCCCATGTACCg                                                                                                                     >  8:27151/1‑136 (MQ=255)
                 cgccATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTAcc                                                                                                                      >  6:129280/1‑135 (MQ=255)
                     aTTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTa                                                                                                                 <  8:341191/136‑1 (MQ=255)
                      ttATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTa                                                                                                                 <  5:49055/135‑1 (MQ=255)
                       tataAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGtt                                                                                                                  >  2:328086/1‑133 (MQ=255)
                          aaGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACag                                                                                                              <  1:112335/134‑1 (MQ=255)
                                 cTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTa                                                                                                                 >  6:88949/1‑124 (MQ=255)
                                 cTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTa                                                                                                                 <  5:88949/124‑1 (MQ=255)
                                    ggCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTc                                                                                                       <  3:18780/131‑1 (MQ=255)
                                    ggCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTc                                                                                                       >  4:18780/1‑131 (MQ=255)
                                    ggCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCa                                                                                                    >  1:248042/1‑134 (MQ=255)
                                    ggCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCa                                                                                                    >  6:81978/1‑134 (MQ=255)
                                          aGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCa                                                                                                                            <  6:113311/104‑1 (MQ=255)
                                          aGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCa                                                                                                                            >  5:113311/1‑104 (MQ=255)
                                            acagATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTg                                                                                            <  6:271721/131‑1 (MQ=255)
                                            aCCGATATTCGCCGAAACGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTg                                                                                            <  2:376094/134‑1 (MQ=255)
                                            aCCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTg                                                                                            <  1:372703/134‑1 (MQ=255)
                                            aCCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTg                                                                                            <  1:214152/134‑1 (MQ=255)
                                            aCCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCAGAGAGTTCGCGATGCCCCATGTACCGGTTACAGAGCttt                                                                                                        <  8:38558/122‑1 (MQ=255)
                                            aCCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCAGAGAGTTCGCGATGCCCCATGTACCGGTTACAGAGCttt                                                                                                        >  7:45069/1‑122 (MQ=255)
                                            aCCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCAGAGAGTTCGCGATGCCCCATGTACCGGTTACAGAGCttt                                                                                                        >  7:38558/1‑122 (MQ=255)
                                            aCCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCAGAGAGTTCGCGATGCCCCATGTACCGGTTACAGAGCttt                                                                                                        <  8:45069/122‑1 (MQ=255)
                                                atatTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAg                                                                                       <  3:371098/135‑1 (MQ=255)
                                                 tatTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTg                                                                                            >  3:385123/1‑129 (MQ=255)
                                                 tatTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTg                                                                                            <  4:385123/129‑1 (MQ=255)
                                                   ttCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACag                                                                                                              <  3:168237/109‑1 (MQ=255)
                                                   ttCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACag                                                                                                              >  4:168237/1‑109 (MQ=255)
                                                   ttCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTg                                                                                            <  5:307982/127‑1 (MQ=255)
                                                   ttCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTg                                                                                            >  6:307982/1‑127 (MQ=255)
                                                   ttCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAg                                                                                    >  5:224514/1‑135 (MQ=255)
                                                   ttCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAg                                                                                    >  2:135436/1‑135 (MQ=255)
                                                   ttCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAg                                                                                    >  6:187833/1‑135 (MQ=255)
                                                   ttCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACAAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAg                                                                                    >  5:343606/1‑135 (MQ=255)
                                                         gAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTggg                                                                               >  4:184879/1‑134 (MQ=255)
                                                          aaaaGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGcc                                                                             <  1:135436/135‑1 (MQ=255)
                                                          aaaaGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGcc                                                                             <  5:112204/135‑1 (MQ=255)
                                                          aaaaGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACAAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGcc                                                                             <  4:217717/135‑1 (MQ=255)
                                                             aGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTgtttcaa                                                                                      >  6:45969/1‑117 (MQ=255)
                                                             aGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTgtttcaa                                                                                      <  5:45969/123‑7 (MQ=255)
                                                               cGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCg                                                                        <  3:90212/135‑1 (MQ=255)
                                                               cGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCg                                                                        <  3:297473/135‑1 (MQ=255)
                                                               cGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCg                                                                        <  3:271828/135‑1 (MQ=255)
                                                               cGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTATCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCg                                                                        <  5:177370/135‑1 (MQ=255)
                                                                          aCTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCGGAGTGGTTGa                                                              <  4:310556/134‑1 (MQ=255)
                                                                                  ggTTCGCCGTGCTTATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGTCAAGGAGCTGGGCCACTCGGAGTGGTTGAAGATCGAcc                                                     <  5:129280/135‑1 (MQ=255)
                                                                                   gTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCGGAGTGGTTGAAGATCGACca                                                    <  3:115877/135‑1 (MQ=255)
                                                                                   gTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCGGAGTGGTTGAAGATCGACca                                                    >  1:251955/1‑135 (MQ=255)
                                                                                                        cTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCGGAGTGGTTGAAGATCGACCAGCAGCGCATCACCCTGTTCGcc                              >  8:386064/1‑136 (MQ=255)
                                                                                                         tttCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCGGAGTGGTTGAAGATCGACCAGCAGCGCATCAACCTGTTCGcc                              <  2:146117/135‑1 (MQ=255)
                                                                                                             gTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCGGAGTGGTTGAAGATCGACCAGCAGCGCATCAACCTGTTCGCCGAgg                          >  5:213629/1‑135 (MQ=255)
                                                                                                             gTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCGGAGTGGTTGAAGATCGACCAGCAGCGCATCAACCTGTTCGCCGAgg                          >  1:369586/1‑135 (MQ=255)
                                                                                                              tACCAACTGACAACCCGGAGAGTCCGCGATGCACAAGGTACCGGTTACAGAGCTTTCGCAGTACGTTGCCAAGCAGCTGGGCCACTCGGAGTGGTTGAAGATCGCCCAGCAGCTCATCAACCTCTTCGCCGAgga                         >  1:281615/1‑134 (MQ=255)
                                                                                                                      gACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCGGAGTGGTTGAAGCTCGACCAGCAGCGCATCAACCTGTTAGCCGCTGCGACCGGCg                 >  2:237414/1‑135 (MQ=255)
                                                                                                                             cGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCGGAGTGGTTGAAGATCGACCAGCAGCGCATCAACCTGTTCGCCGAGGCGACCGGCGATTTCCa          <  2:251955/135‑1 (MQ=255)
                                                                                                                                 gagTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCGGAGTGGTTGAAGATCGACCAGCAGCGCATCAACCTGTTCGCCGAGGCGACCGGCGATTTCCAGTTc      <  5:81978/135‑1 (MQ=255)
                                                                                                                                     ccGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCGGAGTGGTTGAAGATCGACCAGCAGCGCATCAACCTGTTCGCCGAGGCGACCGGCGATTTCCAGTTCATcc  <  1:328086/135‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                      
TCTCCCTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGATATTCGCCGAAAAGCGGGCAACTTGACTGAAACGGTTCGCCGTGCTGATCTTGTACTTTCGTACCAACTGACAACCCGGAGAGTCCGCGATGCCCCATGTACCGGTTACAGAGCTTTCGCAGTACGTTGGCAAGGAGCTGGGCCACTCGGAGTGGTTGAAGATCGACCAGCAGCGCATCAACCTGTTCGCCGAGGCGACCGGCGATTTCCAGTTCATCC  >  ACB122/5471527‑5471794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑