Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,562,567 A→C 20.0% L132R (CTG→CGG)  nnr ← Bifunctional NAD(P)H‑hydrate repair enzyme Nnr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,562,5670AC20.0% 140.1 / 6.9 66L132R (CTG→CGG) nnrBifunctional NAD(P)H‑hydrate repair enzyme Nnr
Reads supporting (aligned to +/- strand):  ref base A (16/34);  new base C (13/2);  total (29/37)
Fisher's exact test for biased strand distribution p-value = 2.47e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAAGGATGCTGCCACTGGGCAGGGCCAGCGGGGTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCACACCCATCAGTTGGTTGGCCGAACGGACACCCAGGCACATGACCTCGGGCAGGCGCGCCAGGCTGGCCGACACGTGCTCGGGCCGGGTTG  >  ACB122/5562435‑5562696
                                                                                                                                     |                                                                                                                                 
gTAAGGATGCTGCCACTGGGCAGGGCCAGCGGGGTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCCCCGCCGCCAACACGCGACAGCCCCACCCCGCCTGCCCCCgg                                                                                                                                 >  4:57203/1‑135 (MQ=255)
      aTGCTGCCACTGGGCAGGGCCAGCGGGGTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGc                                                                                                                           <  3:295751/135‑1 (MQ=255)
      aTGCTGCCACTGGGCAGGGCCAGCGGGGTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGc                                                                                                                           <  3:176031/135‑1 (MQ=255)
      aTGCTGCCACTGGGCAGGGCCAGCGGGCTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGc                                                                                                                           <  4:128348/135‑1 (MQ=255)
       tgctgcCACTGGGCAGGGCCAGCGGGGTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGc                                                                                                                           <  6:271820/134‑1 (MQ=255)
            ccACTGGGCAGGGCCAGCGGGGTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCCCCGCCGCCCCCACGCTACCGCCCCACCCCGCCGGCCCCCGGCCCCgcccgccc                                                                                                                     >  3:350589/1‑135 (MQ=255)
            ccACTGGGCAGGGCCAGCGGGGTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCCCCGCCGCCCACCGGCTACAGCCCCACGCCGCCAGCCCCCCGCCGGgcccgccc                                                                                                                     >  3:123847/1‑135 (MQ=255)
            ccACTGGGCAGGGCCAGCGGGGTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCCCCGCCGAACACCGGCTACAGCCACAAGCAGCCTGCCCCCAGCCGGgcccgccc                                                                                                                     >  6:324792/1‑135 (MQ=255)
            ccACTGGGCAGGGCCAGCGGGGTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGGTGGCCCCCCCCGCCAACCCGCTACAGCCCCCCCCCCCCGGCCCCCGGCCGGgcccgccc                                                                                                                     >  3:90875/1‑135 (MQ=255)
            ccACTGGGCAGGGCCAGCGGGGTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCGCGGGGGTGGGCCCCCCCGCCCACAACCGACACCCCCCCCCCCCCCGCCCCCCGCCGGgcccgccc                                                                                                                     >  6:2243/1‑135 (MQ=255)
            ccACTGGGCAGGGCCAGCGGGGTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCGAGGCGTTGGGCCCCGCCCACCACAGGCTACACCCCCCCGCCGCCGGCCCCCCGCCCGgcccgccc                                                                                                                     >  4:356944/1‑135 (MQ=255)
            ccACTGGGCAGGGCCAGCGGGGTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCGAGGCGTTGGCCACCCCCCCCCACCCGCTACCGACCCCCCCCCCCAGCCCCCGGGCGGGGCCGacc                                                                                                                     >  2:257082/1‑135 (MQ=255)
            ccACTGGGCAGGGCCAGCGGGGTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCGAGGCGGTGTCCCCCCCCGACAACACGGGACAACCCCCCCCCCCCTGCCCCCCGCCGCGGCCGaccc                                                                                                                    >  8:146212/1‑135 (MQ=255)
              aCTGGGCAGGGCCAGCGGGGTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTAGGCGATGGGCCCCGCCCCCCACACGCTACACACCCCCCCCCCCACCCCCACGCCCCGCCCGcccac                                                                                                                   >  5:157888/1‑135 (MQ=255)
                  ggCAGGGCCAGCGGGGTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAgg                                                                                                               <  7:171407/135‑1 (MQ=255)
                   gCAGGGCCAGCGGGGTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGCCCACCGGCTTCCGCCCCCCCCCGCCAGCCCCCCGCCCGGCCCCcccaccccgcc                                                                                                             >  8:311687/1‑131 (MQ=255)
                       ggCCAGCGGGGTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCGCGGCGGTTGGCCCCCCCGCCACCAGGCGGCAGCCCCACGCCCCCTGCCCCAGGCCGGGCCCGGCCACCCCGACCgc                                                                                                          >  4:78376/1‑134 (MQ=255)
                        gCCAGCGGGGTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAg                                                                                                         <  5:191837/135‑1 (MQ=255)
                        gCCAGCGGGGTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAg                                                                                                         <  6:157332/135‑1 (MQ=255)
                        gCCAGCGGGGTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAg                                                                                                         <  1:289735/135‑1 (MQ=255)
                        gCCAGCGGGGTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACATGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAg                                                                                                         <  4:148424/135‑1 (MQ=255)
                         ccAGCGGGGTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGgc                                                                                                       <  7:187110/136‑1 (MQ=255)
                         ccAGCGGGGTGCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGgc                                                                                                       <  7:120951/136‑1 (MQ=255)
                          cAGCGGGGTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGgc                                                                                                       <  1:88805/135‑1 (MQ=255)
                          cAGCGGGGGTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGgc                                                                                                       <  3:373960/135‑1 (MQ=255)
                               gggTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCt                                                                                                  <  3:78376/135‑1 (MQ=255)
                               gggTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTAGGCGTTGGCCACCCCCGACAACCGGCTACAGCCCCCCGCCGCCTGCCCCCGGCCGGGCCCGACCACCCCGCCCGAGgcgcgcca                                                                                                 >  8:294451/1‑134 (MQ=255)
                                 gTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCa                                                                                               <  7:311687/136‑1 (MQ=255)
                                  ttCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCa                                                                                               <  1:172735/135‑1 (MQ=255)
                                  ttCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCa                                                                                               <  6:104054/135‑1 (MQ=255)
                                   gcgcgCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGc                                                                                             <  8:168327/135‑1 (MQ=255)
                                     gcgcCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGca                                                                                            <  1:345514/135‑1 (MQ=255)
                                      cgcCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGca                                                                                            <  3:185222/134‑1 (MQ=255)
                                       gcCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTTGCCCCCGCCGACCACCCGCTACAGCCCCCCGCCGCCGGCCCCACGCCGGGCCCCCCCCCCCCGGCCGCGGCGCGCGCCCGcaccc                                                                                         >  7:78807/1‑134 (MQ=255)
                                          aGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGGTGGGCCCCCCCGCCACCAAGCGACCGCCCCCCCCCCCCGGCCCCCCGCCGGGCCCCCCCCCCCGGACCCCCGCGCGCGCCACCAcccc                                                                                        >  6:178176/1‑134 (MQ=255)
                                                       catcggcatcCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCACACCCATCAGttggttgg                                                                          <  1:110737/135‑1 (MQ=255)
                                                          cggcatcCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCACACCCATCAGTTGGTTGGCCGa                                                                      <  7:46576/136‑1 (MQ=255)
                                                           ggcagcCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCTGGCCGGGCCCGACCACCAGGACAGAGGCGCGCTCCAGCACACCCATCAGTTGGTTGGCCGa                                                                      <  7:363296/135‑1 (MQ=255)
                                                             catcCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCACACCCATCAGTTGGTTGGCCGAAc                                                                    <  3:251654/135‑1 (MQ=255)
                                                                  cacaCTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACCGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCACACCCATCAGTTGGTTGGCCGAACGGacac                                                              <  8:147527/136‑1 (MQ=255)
                                                                  cacaCTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCACACCCATCAGTTGGTTGGCCGAACGGacac                                                              <  8:28600/136‑1 (MQ=255)
                                                                  cacaCTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCACACCCATCAGTTGGTTGGCCGAACGGaca                                                               <  5:149667/135‑1 (MQ=255)
                                                                         gCGGCCGCTCGGCGTGGGCCACCGCCGACAACAGGCTACATCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCACACCCATCAGTTGGTTGGCCGAACGGACACCCAGGc                                                        <  8:155264/135‑1 (MQ=255)
                                                                          cGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCACACCCATCAGTTGGTTCGCCGAACGGACACCCAGGc                                                        <  5:235342/134‑1 (MQ=255)
                                                                                           gcgaCCGCCGCAAACAGGCTACAGGCCCACGCGTCCTTCAGCCTGCCGGGCCCGACGACCAGGACCGAGGCGCGCTCCTCCACACCCCTCAGTTGGTTGGCCGACCGGACACCCAGGCACATCACCTCGGGCAGgc                                      <  8:285531/133‑1 (MQ=255)
                                                                                              aCCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCCGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCACACCCAGCAGTTGGTTGGCCGAACGGACCCCCAGGCACAAGCCCGCGGGCCGgcgcg                                   >  7:78282/1‑136 (MQ=255)
                                                                                              aCCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCACCCCCATCAGTTGGTTGGCCGAACGGGCACCCAGGCACATGACCTCGGGCAGgcgcg                                   >  7:83709/1‑136 (MQ=255)
                                                                                                 gccgACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCACACCCATCAGTTGGTTGGCCGAACGGACACCCAGGCACATGACCTCGGGCAGGCGCGcc                                 <  5:152460/135‑1 (MQ=255)
                                                                                                           ggCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCACACCCATCAGTTGGTTGGCCGAACGGACACCCAGGCACATGACCTCGGGCAGGCGCGCCAGGCTGGCCg                       <  5:195003/135‑1 (MQ=255)
                                                                                                               aCAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGCCCGAGGCGCGCTCCAGCACACCCATCAGTTGGTTGGCCGAACGGACACCCAGGCACATGACCTCGGGCAGGCGCGCCAGGCTGGCCGACACg                  >  7:393274/1‑136 (MQ=255)
                                                                                                               aCAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCCCACCCATCAGTTGGTTGGTCGAACGGACCCCCAGGCCCATGACCTCGGGCAGGCGCGCCAGGCTGGCCGacac                   >  5:9649/1‑135 (MQ=255)
                                                                                                               aCAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCACACCCATCAGTTGGTTGGCCGAACGGACACCCAGGCACATGACCTCGGGCAGGCGCGCCAGGCTGGCCGACACg                  >  8:82920/1‑136 (MQ=255)
                                                                                                                   ccccACGCCGCCTGCCCCAGGCCGGGCCCGCCCACCAGGACCGAGGCGCGCTCCAGCACACCCATCAGTTGGTTGGCCGAACGGACACCCAGGCACATGACCTCGGGCAGGCGCGCCAGGCTGGCCGACACGTGc               >  2:103206/1‑135 (MQ=255)
                                                                                                                     ccACGCCGCCTGCCCCCGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCTCACCCATCAGTTGGTTGGCCGAACGGACACCCAGGCACATGACCTCGGGCAGGAGCTCCAGGCTGGCCGACACGTGCTc             <  1:133895/135‑1 (MQ=255)
                                                                                                                     ccACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCACACCCATCAGTTGGTTGGCCGAACGGACACCCAGGCACATGACCTCGGGCAGGCGCGCCAGGCTGGCCGACACGTGCTc             <  2:44377/135‑1 (MQ=255)
                                                                                                                        cgccgcCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCACACCCATCAGTTGGTTGGCCGAACGGACACCCAGGCACATGACCTCGGGCAGGCGCGCCAGGCTGGCCGACACGTGCTCggg          <  4:350589/135‑1 (MQ=255)
                                                                                                                         gccgccTGCCCCAGGCCGGGCCCGACCACCAGGCCCGAGGCGCGCTCCAGCACACCCATCAGTTGGTTGGCCGAACGGACACCCAGGCACATGACCTCGGGCAGGCGCGCCAGGCTGGCCGACACGTGCTCGGGc         >  3:184134/1‑135 (MQ=255)
                                                                                                                         gccgccTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCACCCCCATCAGTTGGTTGGCCGAACGGACACCCAGGCACATGACCTCGGGCAGGCGCGCCAGGCTGGCCGACACGTGCTCGGGc         >  4:301051/1‑135 (MQ=255)
                                                                                                                         gccgccTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCACACCCATCAGTTGGTTGGCCGAACGGACACCCAGGCACATGACCTCGGGCAGGCGCGCCAGGCTGGCCGACACGTGCTCGGGc         >  2:145395/1‑135 (MQ=255)
                                                                                                                           cgccTGCCCCAGGCCGGGCCCGCCCACCAGGACCGAGGCGCGCTCCAGCACACCCATCAGTTGGTTGGCCGAACGGACACCCAGGCACATGACCTCGGGCAGGCGCGCCAGGCTGGCCGACACGTGCTCGGGCCgg      >  8:344850/1‑136 (MQ=255)
                                                                                                                             ccTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCACACCCATCAGTTGGTTGGCCGAACGGACACCCAGGCACATGACCTCGGGCAGGCGCGCCAGGCTGGCCGACACGTGCTCGGGCCggg     <  1:207498/135‑1 (MQ=255)
                                                                                                                                gCCCCAGGCCGGGCCCGCCCACCAGGACCGAGGCGCGCTCCAGCACACCCATCAGTTGGTTGGCCGACCGGACACCCAGGCCCATGCCCTCGGGCAGGCGCGCCAGGCTGGCCGCCCCGTGCTCGGGCCGGGTTg  >  5:373422/1‑135 (MQ=255)
                                                                                                                                gCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCACACCCATCAgttggt                                                                             >  4:50777/1‑60 (MQ=255)
                                                                                                                                gCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCACACCCATCAgttggt                                                                             <  3:50777/60‑1 (MQ=255)
                                                                                                                                gCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCACACCCATCAGTTGGTTGGCCGAACGGACACCCAGGCACATGACCTCGGGCAGGCGCGCCAGGCTGGCCGACACGTGCTCGGGCCGGGTTg  >  1:16382/1‑135 (MQ=255)
                                                                                                                                gCCCCAGGCCGGCCCCGACCACCAGGACCCCGGCGCGCTCAAGCACACCCATCAGTTGGTTGGCCCAACGGACACCCAGGCACATGACCTCGGGCAGGCGCGCCAGGCTGGCCGACACGTGCTCGGGACGGGTTg  >  3:171960/1‑135 (MQ=255)
                                                                                                                                     |                                                                                                                                 
GTAAGGATGCTGCCACTGGGCAGGGCCAGCGGGGTTCGCGCCAGCAAATTCAAGGCATCGGCATCCCACACTTGCGGCCGCTCGGCGTTGGCCACCGCCGACAACAGGCTACAGCCCCACGCCGCCTGCCCCAGGCCGGGCCCGACCACCAGGACCGAGGCGCGCTCCAGCACACCCATCAGTTGGTTGGCCGAACGGACACCCAGGCACATGACCTCGGGCAGGCGCGCCAGGCTGGCCGACACGTGCTCGGGCCGGGTTG  >  ACB122/5562435‑5562696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: