Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	5,646,451	G→A	7.0%	A225V (GCC→GTC)	pgk ←	Phosphoglycerate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	5,646,451	0	G	A	7.0%	184.5 / 2.4	57	A225V (GCC→GTC)	pgk	Phosphoglycerate kinase
Reads supporting (aligned to +/- strand):  ref base G (32/21);  new base A (2/2);  total (34/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCGGCGCTCTCGGCAAACTCCTTGGCAACCACCACGTCGACTGGCAGCGGCACGCTGACCTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGTCCAGCTTGGTGGAAACCTTGGAGCCGGCAACGATGGCCGCCATTGGCTTGGCCG  >  ACB122/5646320‑5646583                                                                                                                                    |                                                                                                                                     cTTCGGCGCTCTCGGCAAACTCCTTGGCAACCACCACGTCGACTGGCAGCGGCACGCTGACCTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAg                                                                                                                                   >  1:6794/1‑135 (MQ=255) cTTCGGCGCTCTCGGCAAACTCCTTGGCAACCACCACGTCGACTGGCAGCGGCACGCTGACCTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGc                                                                                                                                  >  7:384760/1‑136 (MQ=255) cTTCGGCGCTCTCGGCAAACTCCTTGGCAACCACCACGTCGACTGGCAGCGGCACGCTGACCTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGc                                                                                                                                  >  7:395223/1‑136 (MQ=255)  ttCGGCGCTCTCGGCAAACTCCTTGGCAACCACCACGTCGACTGGCAGCGGCACGCTGACCTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGc                                                                                                                                  <  4:267465/135‑1 (MQ=255)  tgcGGCGCTCTCGGCAAACTCCTTGGCAACCACCACGTCGACTGGCAGCGGCACGCTGACCTTGGCGGCAATGGCCTTGGCGGTGGCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGc                                                                                                                                  <  1:29017/133‑1 (MQ=255)   tCGGCGCTCTCGGCAAACTCCTTGGCAACCACCACGTCGACTGGCAGCGGCACGCTGACCTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCg                                                                                                                                 >  6:339064/1‑135 (MQ=255)                       cTTGGCAACCACCACGTCGACTGGCAGCGGCACGCTGACCTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAAt                                                                                                             >  6:352019/1‑135 (MQ=255)                           gCAACCACCACGTCGACTGGCAGCGGCACGCTGACCTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCg                                                                                                         >  6:75891/1‑135 (MQ=255)                           gCAACCACCACGTCGACTGGCAGCGGCACGCTGACCTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGc                                                                                                        >  8:21418/1‑136 (MQ=255)                             aaCCACCACGTCGACTGGCAGCGGCACGCTGACCTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGcc                                                                                                       >  2:350865/1‑135 (MQ=255)                                  ccacGTCGACTGGCAGCGGCACGCTGACCTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACcga                                                                                                  >  1:109712/1‑135 (MQ=255)                                  ccacGTCGACTGGCAGCGGCACGCTGACCTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACcga                                                                                                  >  4:228100/1‑135 (MQ=255)                                  ccacGTCGACTGGCAGCGGCACGCTGACCTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACcga                                                                                                  >  2:387785/1‑135 (MQ=255)                                    acGTCGACTGGCAGCGGCACGCTGACCTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACcgacg                                                                                                >  5:145177/1‑135 (MQ=255)                                      gTCGACTGGCAGCGGCACGCTGACCTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATc                                                                                             <  7:242322/136‑1 (MQ=255)                                        cGACTGGCAGCGGCACGCTGACCTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCa                                                                                            >  4:381661/1‑135 (MQ=255)                                         gACTGGCAGCGGCACGCTGACCTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGGCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAg                                                                                           <  3:249335/135‑1 (MQ=255)                                             ggCAGCGGCACGCTGACCTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGt                                                                                      >  8:383614/1‑136 (MQ=255)                                                 gCGGCACGCTGACCTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCa                                                                                  <  8:395223/136‑1 (MQ=255)                                                 gCGGCACGCTGACCTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCa                                                                                  <  8:44100/136‑1 (MQ=255)                                                 gCGGCACGCTGACCTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGGGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGc                                                                                   <  6:16423/135‑1 (MQ=255)                                                   ggCACGCTGACCTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCaaa                                                                                <  7:617/136‑1 (MQ=255)                                                       cGCTGACCTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAacac                                                                             <  6:199406/135‑1 (MQ=255)                                                             ccTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGa                                                                       <  5:93212/135‑1 (MQ=255)                                                                    ggcAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGt                                                                 >  2:322860/1‑134 (MQ=255)                                                                    ggcAATGGCCTGGGCGGTGTCCACCAGGGCAGGCTCGGACAGCGACTTGCCCCCCGGCTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTCGTCGCAAACACTGCTGCGGcggat                                                                >  4:387315/1‑131 (MQ=255)                                                                          ggCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCAc                                                          <  2:34209/135‑1 (MQ=255)                                                                          ggCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCAc                                                          <  3:381661/135‑1 (MQ=255)                                                                           gCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGt                                                        >  7:349786/1‑136 (MQ=255)                                                                           gCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGACAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGCCGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGt                                                        >  8:163181/1‑136 (MQ=255)                                                                           gCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGACAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGt                                                        >  8:167781/1‑136 (MQ=255)                                                                                     tgtCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGTCCAGCTtggt                                              <  8:357231/136‑1 (MQ=255)                                                                                     tgtCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGTCCAGCTtggt                                              <  7:210251/136‑1 (MQ=255)                                                                                             aGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGTCCAGCTTGGTGGAAAcc                                       >  3:45853/1‑135 (MQ=255)                                                                                                  aGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGTCCAGCTTGGTGGAAACCTTGGAg                                 >  8:68054/1‑136 (MQ=255)                                                                                                    gCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGTCCAGCTTGGTGGAAACCTTGGAGc                                >  1:22993/1‑135 (MQ=255)                                                                                                      tCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGTCCAGCTTGGTGGAAACCTTGGAGCCg                              >  6:153669/1‑135 (MQ=255)                                                                                                            ggCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACActgctg                                                                        <  1:352787/86‑1 (MQ=255)                                                                                                            ggCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACActgctg                                                                        >  2:352787/2‑87 (MQ=255)                                                                                                            aGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAACCACTGCTGAGGCTGTTCAGCACGTCCAGCTTGGTGGAAACCTTGGAGCCGGCAACGa                       >  7:227624/1‑136 (MQ=255)                                                                                                            aGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGTCCAGCTTGGTGGAAACCTTGGAGCCGGCAACg                        >  6:251148/1‑135 (MQ=255)                                                                                                            aGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGTCCAGCTTGGTGGAAACCTTGGAGCCGGCAACg                        >  5:143806/1‑135 (MQ=255)                                                                                                            aGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGTCCAGCTTGGTGGAAACCTTGGAGCCGGCAACg                        >  6:100892/1‑135 (MQ=255)                                                                                                            aGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGTCCAGCTTGGTGGAAACCTTGGAGCCGGCAACGa                       >  7:82486/1‑136 (MQ=255)                                                                                                              cGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGTCCAGCTTGGTGGAAACCTTGGAGCCGGCAACGAt                      <  1:228212/135‑1 (MQ=255)                                                                                                                 cTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCg                                                                                    <  2:50326/70‑1 (MQ=255)                                                                                                                 cTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCg                                                                                    >  1:50326/1‑70 (MQ=255)                                                                                                                 cTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGTCCAGCTTGGTGGAAACCTTGTAGCCGGCAACGCTGgcc                  >  7:273362/1‑136 (MQ=255)                                                                                                                 cTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGTCCAGCTTGGTGGAAACCTTGGAGCCGGCAACGATGgcc                  >  7:377337/1‑136 (MQ=255)                                                                                                                 cTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGTCCAGCTTGGTGGAAACCTTGGAGCCGGCAACGATGgc                   >  6:221471/1‑135 (MQ=255)                                                                                                                   tGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGATCAGCACGTCCAGCTTGGTGGAAACCTTGGAGCCGGCAACGATGgccg                 <  1:350865/135‑1 (MQ=255)                                                                                                                     cccACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGTCCAGCTTGGTGGAAACCTTGGAGCCGGCAACGATGgccg                 <  5:352019/133‑1 (MQ=255)                                                                                                                     cccACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGTCCAGCTTGGTGGAAACCTTGGAGCCGGCAACGATGGCCGCCa              >  7:104564/1‑136 (MQ=255)                                                                                                                                gCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGTCCAGCTTGGTGGAAACCTTGGAGCCGGCAACGATGGCCGCCATTGGCTTGGcc   <  8:384760/136‑1 (MQ=255)                                                                                                                                 ccGACAGCGGCCAGGAACGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGTCCAGCTTGGTGGAAACCTTGGAGCCGGCAACGATGGCCGCCATTGGCTTGGCCg  <  7:167781/136‑1 (MQ=255)                                                                                                                                 ccGACAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGTCCAGCTTGGTGGAAACCTTGGAGCCGGCAACGATGGCCGCCATTGGCTTGGCCg  <  7:163181/136‑1 (MQ=255)                                                                                                                                  cGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGTCCAGCTTGGTGGAAACCTTGGAGCCGGCAACGATGGCCGCCATTGGCTTGGCCg  <  1:387785/135‑1 (MQ=255)                                                                                                                                   ggCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATc                                                                                             >  7:267152/1‑43 (MQ=255)                                                                                                                                   ggCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATc                                                                                             <  8:267152/43‑1 (MQ=255)                                                                                                                                    |                                                                                                                                     CTTCGGCGCTCTCGGCAAACTCCTTGGCAACCACCACGTCGACTGGCAGCGGCACGCTGACCTTGGCGGCAATGGCCTTGGCGGTGTCCACCAGGTCAGGCTCGTACAGCGACTTGCCCACCGGGTGGCCGGCAGCGGCCAGGAAAGTGTTGGCAATACCGCCACCGACGATCAGCTGGTCGCAAACACTGCTGAGGCTGTTCAGCACGTCCAGCTTGGTGGAAACCTTGGAGCCGGCAACGATGGCCGCCATTGGCTTGGCCG  >  ACB122/5646320‑5646583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑