| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB122 | 5,656,106 | A→C | 21.4% | K68T (AAG→ACG) | mltA → | Membrane‑bound lytic murein transglycosylase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB122 | 5,656,106 | 0 | A | C | 21.4% | 141.8 / 4.2 | 80 | K68T (AAG→ACG) | mltA | Membrane‑bound lytic murein transglycosylase A |
| Reads supporting (aligned to +/- strand): ref base A (36/23); new base C (3/17); total (39/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.49e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGAGAGCGCCAAGCCCGAGCCCCACGCTGTCGCCACCTATGCCCCGGCCACTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCTGCGCTCTGCAGAGAACAATGCCAAC > ACB122/5655975‑5656236 | cgAGAGCGCCAAGCCCGAGCCCCCCGCTGTCGCCACCTATGCCCCGGCCACTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAgcg > 4:390318/1‑135 (MQ=255) gagtGCGCCAAGCCCGAGCCCCACGCTGTCGCCACCTATGCCCCGGCAACTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACGTGCTGGCTGGCTTTTATGCATGGCGCAACGGCTGCTAAAAACTCACgcgc < 2:136553/135‑1 (MQ=255) agCGCCAAGCCCGAGCCCCACGCTGTCGCCACCTATGCCCCGGCCACTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGAcc > 7:184202/1‑136 (MQ=255) ccAAGCCCGAGCCCCACGCTGTCGCCACCTATGCCCCGGCCACTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCgg < 5:97773/135‑1 (MQ=255) cAAGCCCGAGCCCCACGCTGTCGCCCCCTATGCCCCGGCCACGTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCACGCGCGACCCGgt > 5:217474/1‑135 (MQ=255) ccccACGCTGTCGCCACCTATGCCCCGGCCACTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGcc > 5:397290/1‑135 (MQ=255) cccACGCTGTCGCCACCTATGCCCCGGCCACTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCa > 6:161083/1‑135 (MQ=255) cccACGCTGTCGCCACCTATGCCCCGGCCACTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCa > 4:122673/1‑135 (MQ=255) ccACGCTGGCGCCACCTAGGCCCCGGCCACTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCACCCGCGACCCGGTGTGGGCGGCCAc < 6:66047/135‑1 (MQ=255) cTGTCGCCACCTATGCCCCGGCCACTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCg > 7:360862/1‑135 (MQ=255) cTGTCGCCACCTATGCCCCGGCCACTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCg > 7:201917/1‑135 (MQ=255) cTGTCGCCACCTATGCCCCGGCCACTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGCTGTGGGCGGCCACCTGc > 5:340786/1‑134 (MQ=255) aTGCCCCGGCCACTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCa > 3:32858/1‑135 (MQ=255) cccGGCCACTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAGAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAgcgc > 1:136261/1‑135 (MQ=255) ccACTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCAcagc < 7:43065/136‑1 (MQ=255) aCTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCTGCCACCTGCGAAGCGGCCGGCAGCGCCAcag > 2:232836/1‑133 (MQ=255) aCTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCAcagc > 8:314169/1‑134 (MQ=255) aCTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCAcag > 1:179177/1‑133 (MQ=255) aCTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCAcag > 1:152978/1‑133 (MQ=255) aCTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCAcag > 4:335638/1‑133 (MQ=255) aCTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCAcag > 4:219136/1‑133 (MQ=255) aCTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCAcag > 4:147119/1‑133 (MQ=255) aCTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCAcag > 3:85461/1‑133 (MQ=255) aCTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGAGAAGCGGCCGGCAGCGCCAccgc > 8:56034/1‑134 (MQ=255) aCTTGGAAAGACTTGCCGCCCGGCCGCGAGTACGACCGGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGAGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGTGGCCGGCAGCGCCAcagcca < 8:360862/136‑1 (MQ=255) aCTTGGAAAGACTAGCCGCCCGTCAGCGATGAAGACCTGCTCGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGCTGTGGGCGGCCACCTGCGAAGCGGCCGCCAGCGCCAcagcc > 2:386247/1‑135 (MQ=255) cTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCAcagcca > 6:134041/1‑135 (MQ=255) cTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGccc > 8:213139/1‑134 (MQ=255) ccgccCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGt < 3:122673/134‑1 (MQ=255) gccCGTCCGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAACCGCGACCCGGTGGGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCACAAGTGCg < 2:266270/135‑1 (MQ=255) gccCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGc > 1:122074/1‑134 (MQ=255) gccCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCg > 7:395907/1‑135 (MQ=255) gccCGGCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCGGCCAAAAACACACCCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCg < 3:35343/135‑1 (MQ=255) gTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAAcct < 8:184202/136‑1 (MQ=255) gTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAAcct < 7:213139/136‑1 (MQ=255) tCAGCTTTGAAGACGTGGTGGCTGGTTTTTATGCAGGGCCAAAGGGGTGCGAAAAACTCACGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAAcct < 2:152978/135‑1 (MQ=255) tCAGCGATGAAGACCTGGTGGCAGGCTTTTATGCTTGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAAcct < 3:190760/135‑1 (MQ=255) cAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCACGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAAcctt > 3:377102/1‑135 (MQ=255) ttGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTGACGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGa < 7:250535/135‑1 (MQ=255) aggAAGACCTGCTGGCAGGCTTTTATGCAGGGCGCACCGGCTGGGAAAAACGCACGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGa < 8:332756/134‑1 (MQ=255) aCCTGCGGGCAGGCTTTTATGCAGGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGa < 6:364908/135‑1 (MQ=255) ccTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCTAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAAc < 7:264172/136‑1 (MQ=255) ctgctgGCTGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCACGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAAc < 1:37381/135‑1 (MQ=255) ctgctgGCAGGCTTTTATGCATGGCGCACCGGGGGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAAc < 6:350876/135‑1 (MQ=255) tgGCAGGGTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCt < 6:151568/133‑1 (MQ=255) tgGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCt < 4:279813/133‑1 (MQ=255) gCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCACGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAgg < 7:268834/136‑1 (MQ=255) cAGGCTTTTTTGCTTGGCGCACCGGCGGCGAAAACCCCACCCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAgg < 2:379952/135‑1 (MQ=255) cAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAgg < 3:162328/135‑1 (MQ=255) atggtttttATGCCGGGCGAAAGGGGGGCTAAAAACGCACGCGCGACCCGTTGTGGGCTGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCATTGAACAGAACCTGCAgg < 6:217474/130‑1 (MQ=255) aGGCTTTTATGCAGGGCGCAAGGGCTGCCAAAACCTCACGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGgtg < 7:160786/136‑1 (MQ=255) cTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCg < 4:164302/105‑1 (MQ=255) cTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCg > 3:164302/1‑105 (MQ=255) cTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTa > 2:29370/1‑135 (MQ=255) cTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGAGTAc > 8:310286/1‑136 (MQ=255) ttATGCTTGGCGCACGGGATCCAAAAAAGGCACGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACgg < 8:91404/135‑1 (MQ=255) ttATGCAGGGCGCAACGGCTGCGAAAACCTCAACCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACgg < 8:164148/135‑1 (MQ=255) tGCATGGCGCAACGGGTGCGAAAACCTCACGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCt < 8:41372/135‑1 (MQ=255) tGCATGGCGCAACGGCTGCGAAAAACTCACGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCt < 7:187167/135‑1 (MQ=255) tGCAGGGCGCAACGGCGGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCt < 7:391689/135‑1 (MQ=255) gCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCt < 3:147119/134‑1 (MQ=255) aTGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCTgc < 6:337598/134‑1 (MQ=255) aTGGCCCAACGGCGGCGAAAAACTCACGCGCGACCCGGTGGGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCTgc < 2:122074/134‑1 (MQ=255) cgcACCGGCTGCGAAAAACGCATGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGACACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCTGCGctct < 6:76408/135‑1 (MQ=255) cgcACCGCCTGCCAAAAACTCCCGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCTGCGctct < 1:151736/135‑1 (MQ=255) cgcAAGGGGTGCGAAAACCACACGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCTGCGctct < 5:123584/135‑1 (MQ=255) cgcAACGGCTGCTAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCTGCGctct < 3:354292/135‑1 (MQ=255) cgcAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCTGCGctct < 4:268819/135‑1 (MQ=255) cgcAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCTGCGctct > 5:243757/1‑135 (MQ=255) cgcAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCTGCGctct < 5:161083/135‑1 (MQ=255) cgcAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCTGCGctct > 3:268819/1‑135 (MQ=255) cgcAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCTGCGCTCTg > 7:299572/1‑136 (MQ=255) cgcAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCTGCGCTCTg > 7:394478/1‑136 (MQ=255) gcAACGGCTGCGAAAAACTCACGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCTGCGctct > 4:224767/1‑134 (MQ=255) gcAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCTGCGctct > 4:354292/1‑134 (MQ=255) gcAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCTGCGctct > 5:335695/1‑134 (MQ=255) gcAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCTGCGctct > 1:311885/1‑134 (MQ=255) aaaaaCTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCTGCGCTCTGCAGAGAACAATg > 1:50879/1‑135 (MQ=255) aaaaaCTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCTGCGCTCTGCAGAGAACAATGc > 7:258012/1‑136 (MQ=255) cTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCTGCGCTCTGCAGAGAACAATGCCAAc < 3:219136/135‑1 (MQ=255) | CGAGAGCGCCAAGCCCGAGCCCCACGCTGTCGCCACCTATGCCCCGGCCACTTGGAAAGACTTGCCGCCCGTCAGCGATGAAGACCTGCTGGCAGGCTTTTATGCATGGCGCAACGGCTGCGAAAAACTCAAGCGCGACCCGGTGTGGGCGGCCACCTGCGAAGCGGCCGGCAGCGCCACAGCCAGCGCCGCCCAAGTGCGAACCTTCCTTGAACAGAACCTGCAGGTGTACGGCCTGCGCTCTGCAGAGAACAATGCCAAC > ACB122/5655975‑5656236 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |