Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,688,625 A→C 32.0% S232R (AGC→CGC)  pyrC' → Dihydroorotase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,688,6250AC32.0% 29.6 / 2.2 43S232R (AGC→CGC) pyrC'Dihydroorotase‑like protein
Reads supporting (aligned to +/- strand):  ref base A (11/15);  new base C (3/12);  total (14/29)
Fisher's exact test for biased strand distribution p-value = 1.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCC  >  ACB122/5688492‑5688750
                                                                                                                                     |                                                                                                                             
ccAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAg                                                                                                                              <  3:340463/135‑1 (MQ=255)
       ggcCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGACCAGAGCGGCGTGCGTGGGCCCTTGAGACCGAtg                                                                                                                       >  4:340463/1‑134 (MQ=255)
       ggcCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATc                                                                                                                       <  4:124879/135‑1 (MQ=255)
           tGGCCCACGAAGGTGCCATGGCCCGCTTCCTCGGCCTGCCGGGCATCCCGGAGCGCGCCGAAACCGTGGCCCTGGCGCGCACCCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCACGTCAGCCCGCTAACCa                                                                                                                   >  6:145817/1‑135 (MQ=255)
                 aCGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGGGCCCGTCAGCCCCCTCACCACCGccc                                                                                                             >  3:314060/1‑135 (MQ=255)
                     aGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCCCCTCCGCCCGATAACCAGCGCCCGTgg                                                                                                         >  2:126442/1‑135 (MQ=255)
                             tGGCCAGCTTCCTCGGCCGGCCGGGCATCCCGGAGCGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGGGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGc                                                                                                 <  3:382744/135‑1 (MQ=255)
                                  aGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCCCTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCGGATCg                                                                                           >  7:212660/1‑136 (MQ=255)
                                       ccTCGGCCTGCCGGGCATCCAGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGT‑GAACAGAGCGGCGTGGGGGGGCACGTGCGCCATAACCCCCCAGCCCCTGGCGCCCCGCGGATCgccccg                                                                                      >  6:70234/1‑133 (MQ=255)
                                         tCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCGGCGGGTGGAACAGAGCGGCGGGCGTGCGCACTTCCGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCgcccagg                                                                                     <  3:186788/135‑1 (MQ=255)
                                           ggCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCCCGTCCCCCACATCACCCCCGCCCGGGGCGCCCGGGGGGTCgccccggcc                                                                                   >  2:246654/1‑135 (MQ=255)
                                                 ccGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGGGGGCCCTGGAGCCACACCCCCACCGCCCCGGGCGCGCGGGGGCTCgcccccgcccccgcc                                                                             >  3:253619/1‑130 (MQ=255)
                                                            gcagagCGCCTAAACGGGGGCCCGGGCGCGAAACCTGGTGCTGGTGGAACCGAGCGGGGTGCGTGCGCACTTCCGCCAGATCCCCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGcc                                                                  <  3:123163/133‑1 (MQ=255)
                                                                 gcgcCGAACCGGGGGCCGGGGGGCGCACCGTGGGGGTGGTGGAACCGCGGGGGGGGGGGGCGCACTTCCGCCACAGCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                            <  8:364602/136‑1 (MQ=255)
                                                                 gcgcCGAACCGGGGGCCCTGGCGCGCAACCGGCTGCTGGTGTAACCGAGCGGCGTGGGTGCGCACTTCAGCCCTAACCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                            <  7:315396/136‑1 (MQ=255)
                                                                   gcCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTCAGCCAGATCACCCGCCCCCCTGGCGCGCGGCTGCTCGCCCCCGCCCCGGCCGTGGGGCTGCCgggtacc                                                           >  1:32848/1‑130 (MQ=255)
                                                                     cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGGGGGGCCTGGAGCCACCTAACCCGCGCCCGGGGCGCGCGGCGGCTCGCCCCGGCCCCGGCCGTGGGGCGGCCGGGGAccgg                                                         >  5:187535/1‑134 (MQ=255)
                                                                     cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGGGGGGCCGTCAGCCAGATCAACCCCCCCCCGGGCGCGCCGCGGGACGCCCCCGCCCCGGGCGTGGGCCTGCCCGGAAcccc                                                         >  6:359857/1‑133 (MQ=255)
                                                                     cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGGGCACTTCAGCCCGAACACCCCCCCCCCTGGGGCGCGGCGGCTAGCCCCCGCCCACGCCGTGGGCCAGCCCGGAAccgc                                                         >  6:112585/1‑135 (MQ=255)
                                                                       aaaCCGGGGCCCTGGCGCGCAACCTGCTGCTGGGGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGAGCCCCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                       <  3:53954/135‑1 (MQ=255)
                                                                        aaCGGTGGCCGTGGCGGGCAACCGGGTGCGGGTGCACCAGAGCGGCGGGCGGGCGCAGTTCAGCCAGACCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGa                                                      <  4:215227/135‑1 (MQ=255)
                                                                           gggggCCCGGGCGCGAAACCTGCGGGGGGGGCAACAGAGCGGCGGGCGGGCGCACTTCAGCCAGATCCCCAGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                  <  7:257385/133‑1 (MQ=255)
                                                                           cggggCCCTGGCGCGCAACCTGCTGCGGGGGGAACAGAGCGGCGGGCGTGCGCACTTCCGCCACAGCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                  <  8:370936/133‑1 (MQ=255)
                                                                            gTGGCCCTGGCGCGCAACCTGCTGCGGGGGGAACAGCGCGGCGTGCGTGCGCACTTCAGCCAGATCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                  <  2:32848/135‑1 (MQ=255)
                                                                            gTGGCCCTGGCGCGCAACCGGGTGGGGGGGGAACTGAGGGGGGTGCGTGCGCACTGCTGCCAGAGCCCCGGCGCCCGTGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                  <  4:314060/135‑1 (MQ=255)
                                                                                         gcAACCTGGGGCTGGGGAACCAGCGCGGCGGGCGGGCGCACTTCCGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCt                                    <  7:322633/136‑1 (MQ=255)
                                                                                         gaaaCCTGCGGCTGGGGGAACCGGGCGGGGGGCGGGGGCACTTCTGCCAGAGCACCCGGGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGc                                     <  1:246654/133‑1 (MQ=255)
                                                                                             ccTGCTGCTGGTGGAACAGAGCGGCGTGCGAGCGCCCTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGCTCGCCCCGGCCCCGGCCGTGGGCCTGCCCGTAACCGCCGATGTAGCGCTGTACCAGCTGAt                                 >  1:57948/1‑135 (MQ=255)
                                                                                                             cAGAGCGGCGTGCGTGCGCACATCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCCGGCCGTGGGCCTGCCCGTAACCCCCCATGTAGCGCTGTACCAGCTGCTACTCACGGCCGAGTCg                 >  8:319947/1‑135 (MQ=255)
                                                                                                                cgcggcgGGCGTGCGCACTTCCGCCAGAGCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTg              <  1:3372/134‑1 (MQ=255)
                                                                                                                 gcggcgTGCGGGCGCACTCCCCCCAGACCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg            <  7:201757/136‑1 (MQ=255)
                                                                                                                 gcggcgGGCGGGCGCACCCCCGCCACACCCCCGCCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg            <  7:346479/136‑1 (MQ=255)
                                                                                                                  cggcgTGCGTGCGCACTTCAGCCCCAGCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg            <  2:57948/135‑1 (MQ=255)
                                                                                                                      gggcgggcgCACTTCCGCCATATCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt       <  7:319947/134‑1 (MQ=255)
                                                                                                                      gggcgggcgCACTCCAGCCAGATCCCCAGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt       <  8:79315/134‑1 (MQ=255)
                                                                                                                       tgggggcgcACTCCAGCCAGACCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt       <  5:112585/130‑1 (MQ=255)
                                                                                                                       tgcgtgcgAACTGCAGCCAGAGCACCCGGGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt       <  6:125761/135‑1 (MQ=255)
                                                                                                                       ggcgggcgCACTCCCCCCAGAACACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt       <  6:305021/134‑1 (MQ=255)
                                                                                                                        gcgggcgCACTTCCGCCAGAGCACCCGCGCCCGTGCGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGtt      <  4:127569/135‑1 (MQ=255)
                                                                                                                        gcgggcgCACTCCCGCCAGACCACCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTtc     <  8:366354/136‑1 (MQ=255)
                                                                                                                         cgtgccAACTGCAGCCAGAGCCCCCGCGCCCGGGCCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTATCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTtc     <  6:190352/135‑1 (MQ=255)
                                                                                                                         cgggcgcACTTCAGCCACTTCACCCGGGCCGGTGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATGCTCACGGACGAGTCGCTGCGCGAGTtc     <  6:304293/132‑1 (MQ=255)
                                                                                                                           tgcgCAGTTCAGCCAGATCACCAGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc  <  8:84320/136‑1 (MQ=255)
                                                                                                                                     |                                                                                                                             
CCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCC  >  ACB122/5688492‑5688750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑