Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,717,363 A→C 20.6% V753G (GTG→GGG)  opgH ← Glucans biosynthesis glucosyltransferase H

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,717,3630AC20.6% 86.6 / 2.5 54V753G (GTG→GGG) opgHGlucans biosynthesis glucosyltransferase H
Reads supporting (aligned to +/- strand):  ref base A (25/16);  new base C (2/11);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 9.14e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCAGGCCTTTCGGCCCGACTTCAATCGCCTTGGCAACGCGCTCGGCACGCAACGCTTCGATCGGTGCCGCCTGGCCATGGCGGGCAGTGGCCATGGCACAGGCCAGGGCATTCTGCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTTGTGGCGTGGCGTATTCCTCTGGGATAAGGAACAGCTTCTCGTCCTTGGCCGCCAGGC  >  ACB122/5717234‑5717495
                                                                                                                                 |                                                                                                                                     
tCCAGGCCTTTCGGCCCGACTTCAATCGCCTTGGCAACGCGCTCGGCACGCAACGCTTCGATCGGTGCCGCCTGGCCATGGCGGGCAGTGGCCATGGCACAGGCCAGGGCATTCTGCCGCGGGTCGACCACGGcc                                                                                                                                  >  4:359812/1‑135 (MQ=255)
tCCAGGCCTTTCGGCCCGACTTCAATCGCCTTGGCAACGCGCTCGGCACGCAACGCGTCGATCGGTGCCGCCTGGCCATGGCGGGCAGTGGCCATGGCACAGGCCAGGGCATTCTGCCGCGGGTCGCCCACGGccc                                                                                                                                 >  7:138462/1‑136 (MQ=255)
     gCCTTTCGGCCCGACTTCAATCGCCTTGGCAACGCGCTCGGCCCGCAACGCTTCGATCGGTGCCGCCTGGCCATGGCGGGCAGTGGCCATGGCACAGGCCAGGGCATTCTGCCGCGGGTCGACCACGGCCCG‑CAc                                                                                                                            >  1:258384/1‑135 (MQ=255)
     gCCTTTCGGCCCGACTTCAATCGCCTTGGCAACGCGCTCGGCACGCAACGCTTCGATCGGTGCCTCCTGGCCATGGCGGGCAGTGGCCATGGCACCGGCCAGGGCATTCTGCCGCGGGTCGACCACGGCCCG‑CAc                                                                                                                            >  2:222737/1‑135 (MQ=255)
      ccTTTGGGCCCTACTTCAATCGCCTTGGCAACGCGCGCGGCACGCAACGCTTCGAGCGGTGCCGCCTGGCCTTGGCGGGCAGGGGCCATGGCACAGGCCAGGGCATTCTGCCGCGGGTCGACCACGGCCCG‑CACg                                                                                                                           <  6:199388/135‑1 (MQ=255)
       cTTTCGGCCCGACTTCAATCGCCTTGGCAACGCGCTCGGCACGCAACGCTTCGATCGGTGCCGCCTGGCCATGGCGGGCAGTGGCCATGGCACAGGCCAGGGCATTCTGCCGCGGGTCGACCACGGCCCG‑CACg                                                                                                                           >  7:342437/1‑134 (MQ=255)
          tCGCCCCGAATTAAATCGCCTTGCCAACGCGCTCGGCACGAAACGCTTGGATCGGTGCCGCGTGGCCTGGGGGGGAAGGGGCATTGCCAACGGCCTGGGCATTCTGCCGCGGGGCGACCCGGGCCCG‑CACGAAg                                                                                                                        <  8:91237/134‑1 (MQ=255)
             gCCCGACTTCAATCGCTTTGGCAACGCGCTCGGCACGCAACGCTTCGAGCGGTGCCGCCTGGCCATGGCGGGCAGTGGCCATGGCACAGGCCAGGGCATGCTGCCGCGGGTCGACCACGGCCCG‑CACGAAGCcatc                                                                                                                   <  8:138462/136‑1 (MQ=255)
             gCCCGACTTCAATCGCCTTGGCAACGCGCTCGGCACGCAACGCTTCGATCGGTGCCGCCTGGCCATGGCGGGCAGTGGCCATGGCACAGGCCAGGGCATTCTGCCGCGGGTCGCCCCCGGCCCG‑CCCGAAGccct                                                                                                                    >  7:173324/1‑133 (MQ=255)
                    ttCAATCGCCTTGGCAACGCGCTCGGCACGCAACGCTTCGATCGGTGCCGCCTGGCCATGGCGGGCAGTGGCCATGGCACAGGCCAGGGCATTCTGCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAgg                                                                                                            >  8:61007/1‑136 (MQ=255)
                    ttCAATCGCCTTGGCAACGCGCTCGGCACGCAACGCTTCGATCGGTGCCGCCTGGCCATGGCGGGCAGTGGCCATGGCACAGGCCAGGGCATTCTGCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAgg                                                                                                            >  7:14408/1‑136 (MQ=255)
                           gCCTTGGCAACGCGCTCGGCACGCAACGCTTCGATCGGTGCCGCCTGGCCATGGCGGGCAGTGGCCATGGCACAGGCCAGGGCATTCTGCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGcc                                                                                                     >  8:322124/1‑136 (MQ=255)
                            ctttGTCAAGGCGCTCGTAACGAAAGGGTTGTATGGGTGCGGCCTGGCATTGGGGTGCAGTGGCCTTGGCACTGTCCTGGGCATTCTCCCGCGGGTCTACCCCGGCCGG‑CACGAAGCCATCATGCAGGGCATGc                                                                                                      <  6:152458/132‑1 (MQ=255)
                                ggCAACGCGCTCGGCACGCAACGCTTCGATCGGTGCCGCCTGGCCATGGCGGGCAGTGGCCATGGCACAGGCCAGGGCATTCTGCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGc                                                                                                      >  7:76315/1‑130 (MQ=255)
                                ggCAACGCGCTCGGCACGCAACGCTTCGATCGGTGCCGCCTGGCCATGGCGGGCAGTGGCCATGGCACAGGCCAGGGCATTCTGCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGc                                                                                                      <  8:76315/130‑1 (MQ=255)
                                 ccAACGCGCTCGGCCCGAAACGCTTGGATGGGTGCCGCCTGGCATTGGGGGGCTGGGGCCTTGGCACCGGCCAGGGAATTGTGCCGGGGGTCGACCAGGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGt                                                                                               <  8:342437/135‑1 (MQ=255)
                                  cAACGCGATCGCCAGGAAAGGGTTCCATGGTTCCCGCCTGGCAATGGCGGGATTTTGCATTGGAACAGGCACGGGATTTCTGCGGGGGGTCCACCACGGCCCC‑CACGAAGCCATCATGCAGGGCATGCCAGCGGtt                                                                                              <  8:158782/136‑1 (MQ=255)
                                     cgggcgcGGCACCCACCGCTTCGATCGGGGCCGCCTGGCATTGGCGGGCTGTGGCCATGGCACTGGCCAGGGAATTCTGCGGCGGGCCCACCCCGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGtttt                                                                                            <  5:397381/129‑1 (MQ=255)
                                     cgcgcgcgGCACGCAAGGCTTCGATCGGGGCCGCGTGGCCAGGGCGGGCTGGGGCCTTGGCACAGGCCAGGGCATTCTGCGGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGtttt                                                                                            <  6:392184/135‑1 (MQ=255)
                                              cACGCAACGCTTCGATCGGTGCCGCCTGGCCATGGCGGGCAGTGGCCATGGCACAGGCCAGGGCATTCTGCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTa                                                                                  >  7:158782/1‑136 (MQ=255)
                                                 gCAACGCTTGTATCGGTGCCGCGGGGCATTGGGGGGCTGGGGCCAGGGCACTGGCCAGGGAATTCTGCCGCGGGGCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACt                                                                                <  3:171578/135‑1 (MQ=255)
                                                           gagcGGGGCCGCGGGGCCATGGGGGGATGTGGCAATGGAACTGGCCAGGCATTTCTGCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGcc                                                                     <  8:81006/133‑1 (MQ=255)
                                                           gATCGGGGCCGCCGGGCCAGGGCGGGCAGGGGCCAGGGCACAGGCCAGGGCATTCTGCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTa                                                                        <  4:105303/133‑1 (MQ=255)
                                                                        tGGCCTTGGCGGGCAGTGGCCAGGGCACCGGCCAGGGCATGCTGCCGCGGGGCGGCCCCGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTtgt                                                         <  6:68130/135‑1 (MQ=255)
                                                                        tGGCCAGTGGGGGCAGTGGCATGGGCCCAGGCTAGGCTATTGTGCCGCGGGTGCCAAACGGCCCT‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTtgt                                                         <  3:316749/135‑1 (MQ=255)
                                                                        gggCCTTGGCGGGCCGGGGCCTTGGCACAGGCCAGGGCATTCGGCCGGGGGGCGCCCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTtgt                                                         <  4:19442/134‑1 (MQ=255)
                                                                         ggcctgggCGGGCAGTGGCCATGGCACAGGCCAGGGCATTCGGCCGCGGGGCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTtgtg                                                        <  4:167273/129‑1 (MQ=255)
                                                                         ggCCATGGCGGGCAGTGGCCATGGCACAGGCCAGGGCATTCTGCCGCGGGTCGCCCCCGGCCCG‑CACGCAGCCATCATGCAGGGCATGCCAGCGGTGTTCGTGGGTGTACTGGTCGGTAGCCAACCGCTCgtgtt                                                        >  3:19442/1‑134 (MQ=255)
                                                                         ggCCAGGGCGGGCCGGGGCCAGGGCACAGGCCAGGGCATTCGGCCGCGGGGCGCCCCCGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTtgtg                                                        <  6:383/135‑1 (MQ=255)
                                                                           ccttGGCGGGCAGTGGCCATGGCACAGGCCAGGGCATTCTGCCGCGGGGCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTTgtggc                                                      <  2:32239/132‑1 (MQ=255)
                                                                               ggCGGGAAGGGGCGATGGCAAGGGAAAGGCGTTTCTGCCGCGGG‑CGTCCCCGGGCCGTAACGAAGCCATCATGCAGGGCATGCCAGCGGGTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTTgtggcgtggc                                                 <  8:255502/136‑1 (MQ=255)
                                                                                gCGGGCTGGGCCCAGGCCACCGGCCGGGCAAGTCGGCCGGGGGGCGCCCCGGCCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTTgtggcgtggc                                                 <  2:162044/135‑1 (MQ=255)
                                                                                gCGGGCAGTGGCCATGCCACCGGCCTGGGCATCCTGCCGGGGGGCCACCCGGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTTgtggcgtggc                                                 <  3:147580/135‑1 (MQ=255)
                                                                                gCGGGCAGGGGCCATGGCACCGGCCAGGGCATTCTGCCGCGGGGCGACCCCGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTTgtggcgtggc                                                 <  3:248929/135‑1 (MQ=255)
                                                                                       gTGGCCATGGCACAGGCCAGGGCATTCTGCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAGTGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTTGTGGCGTGGCGTATTcc                                          >  3:260401/1‑135 (MQ=255)
                                                                                       gTGGCCATGGAACAGGCCAGGGAATTCTGCCGCGGGGCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTTGTGGCGTGGCGTATTc                                           <  4:334977/134‑1 (MQ=255)
                                                                                                  acaGGCCAGGGCATTCTGCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGgt                                                                                     <  7:2625/81‑1 (MQ=255)
                                                                                                  acaGGCCAGGGCATTCTGCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGgt                                                                                     >  8:2625/1‑81 (MQ=255)
                                                                                                       ccAGGGCATTCTGCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTTGTGGCGTGGCGTATTCCTCTGGGATAAGGAACa                          >  6:170035/1‑135 (MQ=255)
                                                                                                        cAGGGCATTCTGCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTTGTGGCGTGGCGTATTCCTCTGGGATAAGGAACAGc                        <  8:14408/136‑1 (MQ=255)
                                                                                                         aGGGCATTCTGCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTTGTGGCGTGGCGTATTCCTCTGGGATAAGGAACAGc                        >  6:134195/1‑135 (MQ=255)
                                                                                                            gCATTCTGCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTTGTGGCGTGGCGTATTCCTCTGGGATAAGGAACAGCTtc                     >  3:340176/1‑135 (MQ=255)
                                                                                                              aTTCTGCCGCGGGTCGCCCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTTGTGGCGTGGCGTATTCCTCTGGGATAAGGAACAGCTtctc                   >  5:12826/1‑135 (MQ=255)
                                                                                                              aTTCTGCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTTGTGGCGTGGCGTATTCCTCTGGGATAAGGAACAGCTTCTCg                  >  7:239986/1‑136 (MQ=255)
                                                                                                                  ggCCGCGGGTCGACCCCGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTTGTGGCGTGGCGTATTCCTCTGGGATAAGGAACAGCTTCTCGTCCt              <  8:40643/135‑1 (MQ=255)
                                                                                                                   gCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCACCAGCTCTTGTGGCGTGGCGTATTCCTCTGGGGTAAGGAACCGCTTCTCGTCCt              >  1:172701/1‑135 (MQ=255)
                                                                                                                   gCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACCGCTCTTGTGGCGTGGCGTATTCCTCTGGGATAAGGAACAGCTTCTCGTCCt              >  4:74253/1‑135 (MQ=255)
                                                                                                                   gCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTTGTGGCGTGGCGTATTCCTCTGGGATAAGGAACAGCTTCTCGTCCtt             >  7:82240/1‑136 (MQ=255)
                                                                                                                   gCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTTGTGGCGTGGCGTATTCCTCTGGGATAAGGAACAGCTTCTCGTCCt              >  5:181993/1‑135 (MQ=255)
                                                                                                                   gCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTTGTGGCGTGGCGTATTCCTCTGGGATAAGGAACAGCTTCTCGTCCt              >  4:315159/1‑135 (MQ=255)
                                                                                                                   gCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTTGTGGCGTGGCGTATTCCTCTGGGATAAGGAACAGCTTCTCGTCCt              >  2:14559/1‑135 (MQ=255)
                                                                                                                   gCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTTGTGGCGTGGCGTATTCCTCTGGGATAAGGAACAGCTTCTCGTCCt              >  1:5925/1‑135 (MQ=255)
                                                                                                                     cgcgGGTCGACCCCGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTTGTGGCGTGGCGTATTCCTCTGGGATAAGGAACAGCTTCTCGTcc               <  6:144154/132‑1 (MQ=255)
                                                                                                                          gTCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTAGTGGTCGGTAGCCAACAGCTCTTGTGGCGTGGCGTATTGGGGGGGGATAAGGAACAGCTTCTCGTCCTTGgccgc       >  1:146554/1‑135 (MQ=255)
                                                                                                                                 aCGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTTGTGGCGTGGCGTATTCCTCGGGGATAAGGAACAGCTTCTCGTCCTTGGCCGcccggc  >  1:387436/1‑133 (MQ=255)
                                                                                                                                 |                                                                                                                                     
TCCAGGCCTTTCGGCCCGACTTCAATCGCCTTGGCAACGCGCTCGGCACGCAACGCTTCGATCGGTGCCGCCTGGCCATGGCGGGCAGTGGCCATGGCACAGGCCAGGGCATTCTGCCGCGGGTCGACCACGGCCCG‑CACGAAGCCATCATGCAGGGCATGCCAGCGGTTTTCGTGGGTGTACTGGTCGGTAGCCAACAGCTCTTGTGGCGTGGCGTATTCCTCTGGGATAAGGAACAGCTTCTCGTCCTTGGCCGCCAGGC  >  ACB122/5717234‑5717495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑