Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,773,403 T→G 22.5% F334V (TTC→GTC)  nhaP2_2 → K( )/H( ) antiporter NhaP2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,773,4030TG22.5% 90.6 / 2.7 53F334V (TTC→GTC) nhaP2_2K( )/H( ) antiporter NhaP2
Reads supporting (aligned to +/- strand):  ref base T (15/24);  new base G (11/3);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.35e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCCTGGTGGCGCGGCCACTGTCGGTGGTCGCGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGCCCAGCTGTTCTTCAACCTGGCGTTCTTCATCGTGCTGGTGTCACTGCTGGTGCAG  >  ACB122/5773273‑5773537
                                                                                                                                  |                                                                                                                                      
gccgcACGACGTGCGGCCCATCGCCCTGCCGGCACTGGGCCGGGCGCTGGGGATGATCCTGGTGGCGCGGCCACTGTGGGTGGGCGCGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCAt                                                                                                                                    <  4:368505/135‑1 (MQ=255)
gccgcACGACCGGCTGCCCATCGCCCTGCCGGCACGGGGCCGGGCGCTGTGTATGATCCTGGTGGGGCGGCCACTGGCGGTGGGCGCGGCCCTGCTGCCTTTCACGGCTTTCCACGGCCGTGAAAAGGGCTTCAt                                                                                                                                    <  4:209996/135‑1 (MQ=255)
     aCGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCCTGGTGGCGCGGCCACTGTCGGTGGTCGCGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTg                                                                                                                              >  7:262306/1‑136 (MQ=255)
     aCGACCTGCTCCCCATCGCCCGGCGGGCTGTGGGCGTGGCGGTGTGTTTTTTCGGGGTGGCGCGGCCTGTGGCGGTGGTGGGGGCCGTGGTGCCTTACTTGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGt                                                                                                                               <  6:383081/135‑1 (MQ=255)
     aCGACCTGCGGCGAATCGGCCTGAGGGCACTGGGCGTGGGGCGGTGTATGATCCTGGTGGCGCGCCCACGGGGGGTGGTCGCGGCCCTGCTGCGTTTCATGGGTTTCCACGGCAGTGAAAAGGGCTTCATTTCGt                                                                                                                               <  2:263735/135‑1 (MQ=255)
     aCGACCTGCGGCCCATCGCCCTGCCGGCACGGGGCCTGGCGCGGTGGATGATCCTGGGGGCGCGGCCACTGTCGGTGGGCGCGGCCCTGCTGCCTTTCACGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGt                                                                                                                               <  4:228870/135‑1 (MQ=255)
       gACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCCTGCTGGCGCGGACACTGTCGGTGGTCGCGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGTGCTTCAATTAGTgt                                                                                                                             >  2:34021/1‑134 (MQ=255)
         ccTGCTGCCCACCGCCCGGCCGGCACTGGGCCTGGCGCTGTGGATGATCCTGGTGGCGCGGCCAGTGGCGGTGGGCGCGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTc                                                                                                                          <  8:393929/136‑1 (MQ=255)
               gccccgcGCCCTGCCGGCACTGGGCCGGGCGCGGTGGATGATCCTGGGGGCGCGGCCACTGGCGGTGGGCGCGGCCCTGCGGCCTTTCACGGCTTCCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCt                                                                                                                     <  1:276456/129‑1 (MQ=255)
                 ccATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCCTGGTGGCGCGGCCACTGTCGGTGGTCGCGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCGTCCTTTCGTGGGTCGGCCTgc                                                                                                                   >  3:347085/1‑135 (MQ=255)
                   agcGCCCGGCCGGCACGGGGCCTGGCGCTGGGGATGATCCTGGGGGCGCGGCCACTGGCGGTGGTCGCGGCCCTGCTGCCTTTCACGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTgcgcg                                                                                                                <  7:96661/134‑1 (MQ=255)
                   agcGCCCGGCCGCCACGGGGCCGGGCGCTGTGGATGATCCGGGTGGCGCGGCCAGGGGCGGGGGGCGCGGCCCTGCTGCCTTTCACGGCTTCCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTgcgcg                                                                                                                <  7:170571/134‑1 (MQ=255)
                      gCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCCTGGTGGCGCGGCCACTGTCGGTGGTCGCGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGGGTCATTTCGTGGGTCGGCCTgcgcggc                                                                                                              >  2:291578/1‑135 (MQ=255)
                      gCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCCTGGTGGCGCGGCCACTGTCGGTGGTCGCGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTgcgcggcg                                                                                                             >  8:203583/1‑136 (MQ=255)
                      gCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCCTGGTGGCGCGGCCACTGTCGGTGGTCGCGGCCCGGCTGCCTTTCAAGGCTTTCCACGGCCGTGGAAACGGCGGGCGTTGGTGGGGCGGCCTgcgcggc                                                                                                              >  6:365792/1‑135 (MQ=255)
                             cGGCAGGGGGCCTGGCGCTGTGGATGATCCTGGTGGCGCGGCCACTGTCGGTGGTCGCGGCCCTGCGGCCTTTCACGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTAcc                                                                                                      <  7:109693/136‑1 (MQ=255)
                             cGGCACTGGGCCTGGCGCTGTGGATGATCCTGGTGGCGCGGCCACTGTCGGTGGTCGCGGCCCTGCTGCCTTTCACGGCTTTCCACGGCCGTGGAAACGGGTTGAGTTGGTGGGGGGGGCGGcgcggcgcggggc                                                                                                       >  5:276412/1‑132 (MQ=255)
                              ggCACGGGGCCTGGCGCTGTGGATGATCCTGGGGGCGCGGCCACTGTCGGGGGTCGCGGCCGTGCTGCCTTTCACGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTAcc                                                                                                      <  1:296430/135‑1 (MQ=255)
                                cACTGGGCGTGGCGCGGTGGATGATCCTGGGGGCGCGGCCAGTGTCGGTGGTCGCGGCCCTGCTGCCTTTCACGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGa                                                                                                    <  2:224863/135‑1 (MQ=255)
                                    gggCCTGGCGCTGTGGATGATCCTGGTGGCGCGGCCACTGTCGGTGGTCGCGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGGGTCATTTAGTGGGTCGGCCTGCGCGGCGCGGTACCGATcc                                                                                                 >  6:350749/1‑133 (MQ=255)
                                       ccTGGCGCTGTGGATGATCCTGGTGGCGCGGCCACTGTCGGTGGTCGCGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGGGCATTCt                                                                                             >  2:365971/1‑135 (MQ=255)
                                       ccTGGCGCTGTGGATGATCCTGGTGGCGCGGCCACTGTCGGTGGTCGCGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTg                                                                                            <  7:203583/136‑1 (MQ=255)
                                        cTGGCGCTGTGGATGATCCTGGTGGCGCGGCCACTGTCGGTGGTCGCGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTGGTGGGTCGGCCGGCGCGGCGCGGTACCGCTCATTCTg                                                                                            >  2:364922/1‑135 (MQ=255)
                                        cTGGCGCTGTGGATGATCCTGGTGGCGCGGCCACTGTCGGTGGTCGCGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCGGCGCGGCGCGGGTCCGATCATTATg                                                                                            >  5:280612/1‑135 (MQ=255)
                                        cTGGCGCTGGGGATGATCCTGGGGGCGCGGCCACTGTCGGTGGGCGCGGCCCGGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTg                                                                                            <  5:172293/135‑1 (MQ=255)
                                             gcTGTGGATGATCCTGGTGGCGCGGCCACTGTCGGTGGTCGCGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGGAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGCTCATTCTTGCggg                                                                                       >  2:256455/1‑134 (MQ=255)
                                             gcTGTGGATGATCCTGGTGGCGCGGCCACTGTCGGTGGTCGCGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCGTCATTTCGTGGGTCGGCCTGCGCGGCGCGGGACCGATCATTCTGGCGgtg                                                                                      >  7:173281/1‑136 (MQ=255)
                                                  ggATGATCCTGGTGGCGCGGCCACTGTCGGTGGTCGCGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAATGGGTTAATTTCGTGGGTCGGCCTGCGCGGCGCGGGACCGGTCATTCTGGCGGTGttac                                                                                  >  5:349013/1‑133 (MQ=255)
                                                    atgatCCTGGTGGCGCGGCCACTGTCGGTGGTCGCGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGGAAAAGGCGTGATTTCGTGGGGGGGCCTGCGCGGCGCGGGTCCGCTCATTCTGGCGGGGTTCCCg                                                                                >  1:396682/1‑135 (MQ=255)
                                                    atgatCCTGGTGGCGCGGCCACTGTCGGTGGTCGCGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTAGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCg                                                                                >  1:231111/1‑135 (MQ=255)
                                                         ccTGGTGGCGCGGCCACTGTCGGTGGTCGCGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGGTGGATTTGGTGGGTCGGCCGCGGCGGCGCGGGCCCCATGAATGTGGCGGGGTTGCCGcgggt                                                                           >  5:290148/1‑131 (MQ=255)
                                                              tggCGCGGCCACTGTCGGTGGTCGCGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGGAAAGGGCGTCATTTGGTGGGTCGGCCGGCGCGGCGCGGTTCCGCTCATTCTGGCGGTGTTGCCGCTGATGGCgg                                                                      >  6:392891/1‑135 (MQ=255)
                                                                gcgcGGCCACTGTCGGTGGTCGCGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGGAAAGGGGGTGCTTTAGTGGGGGGGCCGGGGCGGCGGGGGTCCGCTCCTTCTGGGGGGGTTGCCGCTGGTgggggggc                                                                   >  8:343681/1‑134 (MQ=255)
                                                                    ggCCCCGGTCGGGGGGCGCGGCCCGGCGGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGc                                                                <  4:299712/135‑1 (MQ=255)
                                                                      ccACTGTCGGTGGTCGCGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGGAAAATGCGTCATTTAGTGGGTGGGGCGGCGCGGCGCCGGACCGGTAATTAGGGGGGTGTTTCCGGGGATTGCCGGcccggtcg                                                             >  8:339357/1‑130 (MQ=255)
                                                                                       ggCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGGAAAGGGCTTGTTTTCGTGGGTCGGCCTGCGCGGCGCGGGACCGGTCATTCTGGCGGTGGTGCCGCGGGTGGCGGGCCGGCCAGACGCCCCGCGGtgctt                                             >  2:103330/1‑135 (MQ=255)
                                                                                         cccTGGGGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGCCCAGCTGTTCTTCa                                           <  1:239560/135‑1 (MQ=255)
                                                                                         cccTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCGTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCCGACGCCCAGCTGTTCTTCa                                           >  2:103986/1‑135 (MQ=255)
                                                                                         cccTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAAGGCGTCATTTCGTGGGTCGGCCGGCCCGGCGCGGTACCGCTCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCGGCCCGACGCCCCGCGGGTCGTCa                                           >  5:218267/1‑135 (MQ=255)
                                                                                                  cttgaaaGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGCCCAGCTGTTCTTCAACCTGGc                                    <  5:51480/128‑1 (MQ=255)
                                                                                                     tCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGCCCAGCTGTTCTTCAACCTGGCGttct                               <  8:262306/135‑1 (MQ=255)
                                                                                                      ctaGGCTTGCGACGGGCGTGAATAGGGCTTCATTTCGTGGGGCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGCCCAGCTGTTCTTCAACCTGGCGttct                               <  2:195707/132‑1 (MQ=255)
                                                                                                      cAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGCCCAGCTGGTCTTCAACCGGGCGgtct                               >  4:124876/1‑134 (MQ=255)
                                                                                                        tggCTTCCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGCCCAGCTGTTCTTCAACCTGGCGTTCTTCa                            <  2:120086/134‑1 (MQ=255)
                                                                                                            ttcccACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGCCCAGCTGTTCTTCAACCTGGCGTTCTTCATCGt                        <  5:392891/132‑1 (MQ=255)
                                                                                                               ccACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGCCCAGCTGTTCTTCAACCTGGCGTTCTTCATCGTg                       <  1:256455/133‑1 (MQ=255)
                                                                                                                 aCGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTTCGCGGCGCGGTAACGATCATTCTGGCCGTGTTCCCGCTGATGGCGGGCCAGCCAGACGCCCAGCTGTTCTTCAACCTGGCGTTCTTCATCGTGCTGgt                  >  8:64779/1‑136 (MQ=255)
                                                                                                                    gCCGTGAAAAGGGCGTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGCCCAGCTGTTCTTCAACCTGGCGTTCTTCATCGTGCTGgtgt                <  4:8910/135‑1 (MQ=255)
                                                                                                                           aaaGGGCTTCATTTCGTGGGTAGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTACCGCGGATGGCGGGCCTGCCAGACGCCCCGCTGTTATTAACACAGGCGTTGTTGATCGTGCTGGTGTCAcagctt        >  8:11993/1‑135 (MQ=255)
                                                                                                                           aaaGGGCGTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCGGATGGCGGGCGTGCCAGACGCCCAGCTGTTCTTCAACCTGGCGTTCTTCATCGTGCTGGTGTCActgctg        <  7:240710/136‑1 (MQ=255)
                                                                                                                            aaGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGCCCAGCTGTTCTTCAACCTGGCGTTCTTCATCGTGCTGGTGTCActgctg        <  2:11701/135‑1 (MQ=255)
                                                                                                                            aaGGGCTTCATTTCGTGGGGCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGCCCAGCTGTTCTTCAACCTGGCGTTCTTCATCGTGCTGGTGTCActgctg        <  5:273745/135‑1 (MQ=255)
                                                                                                                              gggCGTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGCCCAGCTGTTCTTCAACCTGGCGTTCTTCATCGTGCTGGTGTCACTGCTGGt      <  1:300222/135‑1 (MQ=255)
                                                                                                                                  ttCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGCCCAGCTGTTCTTCAACCTGGCGTTCTTCATCGTGCTGGTGTCACTTCTGGTGCAg  >  1:340422/1‑135 (MQ=255)
                                                                                                                                  |                                                                                                                                      
GCCGCACGACCTGCTGCCCATCGCCCTGCCGGCACTGGGCCTGGCGCTGTGGATGATCCTGGTGGCGCGGCCACTGTCGGTGGTCGCGGCCCTGCTGCCTTTCAAGGCTTTCCACGGCCGTGAAAAGGGCTTCATTTCGTGGGTCGGCCTGCGCGGCGCGGTACCGATCATTCTGGCGGTGTTCCCGCTGATGGCGGGCCTGCCAGACGCCCAGCTGTTCTTCAACCTGGCGTTCTTCATCGTGCTGGTGTCACTGCTGGTGCAG  >  ACB122/5773273‑5773537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑