Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 5,917,508 G→A 6.6% G53G (GGC→GGT)  gcvH_2 ← Glycine cleavage system H protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1225,917,5080GA 6.6% 185.5 / 2.3 61G53G (GGC→GGT) gcvH_2Glycine cleavage system H protein
Reads supporting (aligned to +/- strand):  ref base G (40/16);  new base A (2/2);  total (42/19)
Fisher's exact test for biased strand distribution p-value = 5.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCTCTTCGTTCAGCAGTTCCGGGCTATCGGCCAGCTCTTCGTTGACCGCAATCACTTCGCCACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATACCCACGGTCACACTGCCGTCGGCTTCCAGGCGAGCCCACTCATGGCTTTCGGCAAA  >  ACB122/5917380‑5917639
                                                                                                                                |                                                                                                                                   
ggCTCTTCGTTCAGCAGTTCCGGGCTATCGGCCAGCTCTTCGTTGACCGCAATCACTTCGCCACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAac                                                                                                                               <  1:272086/135‑1 (MQ=255)
ggCTCTTCGTTCAGCAGTTCCGGGCTATCGGCCAGCTCTTCGTTGACCGCAATCACTTCGCCACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAac                                                                                                                               >  4:118999/1‑135 (MQ=255)
ggCTCTTCGTTCAGCAGTTCCGGGCTATCGGCCAGCTCTTCGTTGACCGCAATCACTTCGCCACCGACCGGCGCATAGATGTCCGAAGCGGCCTTGACCGCCTCGCCCACACCCGCAGCGTCGCCAGCGCCaccc                                                                                                                               >  6:346802/1‑132 (MQ=255)
        gTTCAGCAGTTCCGGGCTATCGGCCAGCTCTTCGTTGACCTCAATCACTTCGCCCCCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAACCCCTTTGcc                                                                                                                       >  2:260610/1‑135 (MQ=255)
        gTTCAGCAGTTCCGGGCTATCGGCCAGCTCTTCGTTGACCGCAATCACTTCGCCACCGCCCGGGGCATAGATGTCCGAAGCGGCCTTGACCGCCTCGACCCCACCCGCAGCGTCGCCAGCGCCAAACACTTTGcc                                                                                                                       >  6:302720/1‑135 (MQ=255)
        gTTCAGCAGTTCCGGGCTATCGGCCAGCTCTTCGTTGACCGCAATCACTTCGCCACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGcc                                                                                                                       >  6:94356/1‑135 (MQ=255)
        gTTCAGCAGTTCCGGGCTATCGGCCAGCTCTTCGTTGACCGCAATCACTTCGCCACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGcc                                                                                                                       >  6:236180/1‑135 (MQ=255)
                    cGGGCTATCGGCCAGCTCTTCGTTGACCGCAATCACTTCGCCACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTcagccag                                                                                                           >  5:367164/1‑135 (MQ=255)
                    cGGGCTATCGGCCAGCTCTTCGTTGACCGCAATCACTTCGCCACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTcagccag                                                                                                           >  8:169783/1‑135 (MQ=255)
                    cGGGCTATCGGCCAGCTCTTCGTTGACCGCAATCACTTCGCCACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTcagcca                                                                                                            <  6:367164/134‑1 (MQ=255)
                      ggCTATCGGCCAGCTCTTCGTTGACCGCAATCACTTCGCCACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCt                                                                                                         >  4:142220/1‑135 (MQ=255)
                         tATCGGCCAGCTCTTCGTTGACCGCAATCACTTCGCCACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTcagcca                                                                                                            >  1:216110/1‑129 (MQ=255)
                         tATCGGCCAGCTCTTCGTTGACCGCAATCACTTCGCCACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTcagcca                                                                                                            <  2:216110/129‑1 (MQ=255)
                         tATCGGCCAGCTCTTCGTTGACCGCAATCACTTCGCCACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTcagcc                                                                                                             <  5:155921/128‑1 (MQ=255)
                         tATCGGCCAGCTCTTCGTTGACCGCAATCACTTCGCCACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTcagcc                                                                                                             >  6:155921/1‑128 (MQ=255)
                         tATCGGCCAGCTCTTCGTTGACCGCAATCACTTCGCCACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTcgac                                                                                                     >  7:2592/1‑136 (MQ=255)
                         tATCGGCCAGCTCTTCGTTGACCGCAATCACTTCGCCACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCAAGCGCCAAACACTTTGCCGACTTCAGCCAGCTcga                                                                                                      >  5:338639/1‑135 (MQ=255)
                              gCCAGCTCTTCGTTGCACGCCATCACTTCGCCACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGCCTCGCCCACACCCGCCGCGTCGCCAGCGCCAAACACTTTGCCGACTTCCGACAGCTCGCCGaaga                                                                                                >  7:27116/1‑134 (MQ=255)
                              gCCAGCTCTTCGTTGACCGCAATCACTTCGCCACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGCCCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGCCGAATa                                                                                                >  8:86404/1‑136 (MQ=255)
                                 aGCTCTTCGTTGAACGCAATCACTTCGCCACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGAACACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATAcc                                                                                              <  3:94592/135‑1 (MQ=255)
                                      ttCGTTGACCGCAATCACTTCGCCACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACCCCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATc                                                                                         >  6:391921/1‑135 (MQ=255)
                                           tGACCGCAATCACTTCGCCACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCAcc                                                                                      >  4:146971/1‑133 (MQ=255)
                                                gCAATCACTTCGCCACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCACCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCt                                                                               >  6:182169/1‑135 (MQ=255)
                                                           gCCACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTg                                                                    >  5:50581/1‑135 (MQ=255)
                                                           gCCACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCACCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCACGGCTTCCTGGGCg                                                                      <  5:182169/133‑1 (MQ=255)
                                                            ccACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGa                                                                   >  3:191744/1‑135 (MQ=255)
                                                               ccgaccgGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCCCCCAAGGCTTCCTGGGCGTGCTCg                                                                >  5:353993/1‑135 (MQ=255)
                                                               ccgaccgGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCg                                                                >  2:132059/1‑135 (MQ=255)
                                                               ccgaccgGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGc                                                               >  7:171858/1‑136 (MQ=255)
                                                                    cgGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGAt                                                           <  5:120874/135‑1 (MQ=255)
                                                                     ggggCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATAc                                                         >  7:237269/1‑136 (MQ=255)
                                                                     ggggCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATAc                                                         <  7:169783/136‑1 (MQ=255)
                                                                         cATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACtt                                                                                                                           <  1:200360/66‑1 (MQ=255)
                                                                         cATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACtt                                                                                                                           >  2:200360/1‑66 (MQ=255)
                                                                          aTAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCACCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATAc                                                         >  2:216052/1‑131 (MQ=255)
                                                                          aTAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCACCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATAc                                                         <  1:216052/131‑1 (MQ=255)
                                                                                    gAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATACCCACGGTCACACTGc                                          >  7:56659/1‑136 (MQ=255)
                                                                                    gAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGATCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATACCCACGGTCACACTg                                           >  4:227809/1‑135 (MQ=255)
                                                                                       gCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATACCCACGGTCACACTGCCg                                        <  5:236180/135‑1 (MQ=255)
                                                                                       gCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATACCCACGGTCACACTGCCg                                        <  4:241599/135‑1 (MQ=255)
                                                                                         ggCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGCCTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATACCCACGGTCACACTGCCGTCg                                     >  7:272332/1‑136 (MQ=255)
                                                                                              tGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTACTGGGCGTGATCGCTGATACCCACGGTCACACTGCCGTCGGCTTc                                >  8:14247/1‑136 (MQ=255)
                                                                                                 ccGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATACCCACGGTCACACTGCCGTCGGCTTCCAg                             >  8:377513/1‑136 (MQ=255)
                                                                                                 cagACTCGACCACACCAGCAGCGTCGCCAGATCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATACCCACGGTCACACTGCCGTCGGCTTcc                               <  1:297593/132‑1 (MQ=255)
                                                                                                    aCTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATACCCACGGTCACACTGCCGTCGGCTTCCAGGCg                          <  7:377513/136‑1 (MQ=255)
                                                                                                     cTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATACCCACGGTCACACTGCCGTCGGCTTCCAGGCg                          <  3:142220/135‑1 (MQ=255)
                                                                                                       cGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGTCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATACCCACGGTCACACTGCCGTCGGCTTCCAGGCGAg                        >  5:23901/1‑135 (MQ=255)
                                                                                                        gACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGCTACCCACGGTCACACTGCCGTCGGCTTCCAGgcgcgc                       >  1:292653/1‑135 (MQ=255)
                                                                                                        gACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATAc                                                         <  7:105122/101‑1 (MQ=255)
                                                                                                        gACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATAc                                                         >  8:105122/1‑101 (MQ=255)
                                                                                                        gACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATACCCACGGTCACACTGCCGTCGGCTTCCAGGCGAGcc                      >  7:394770/1‑136 (MQ=255)
                                                                                                        gACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATACCCACGGTCACACTGCCGTCGGCTTCCAGGCGAGcc                      >  8:8691/1‑136 (MQ=255)
                                                                                                        gACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATACCCACGGTCACACTGCCGTCGGCTTCCAGGCGAGc                       >  2:25515/1‑135 (MQ=255)
                                                                                                           cacaCCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACCTCACCCAAGGCTTCCTGGGCGTGATCGCTGATACCCACGGTCACACTGCCGTCGGCTTCCAGGCGAGCCCAc                   <  8:152046/136‑1 (MQ=255)
                                                                                                            acacCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATACCCACGGTCACACTGCCGTCGGCTTCCAGGCGAGCCCAc                   >  6:354083/1‑135 (MQ=255)
                                                                                                                   cAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGAt                                                           >  2:32363/1‑88 (MQ=255)
                                                                                                                   cAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGAt                                                           <  1:32363/88‑1 (MQ=255)
                                                                                                                      cGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATACCCACGGTCACACTGCCGTCGGCTTCCAGGCGAGCCCACTCATGGCttt         >  1:125974/1‑135 (MQ=255)
                                                                                                                        tCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATACCCACGGTCACACTGCCGTCGGCTTCCAGGCGAGCCCACTCATGGCTTTCg       <  3:146971/135‑1 (MQ=255)
                                                                                                                           ccAGCGCCAAACACTTTGCCGCCTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATACCCACGGTCACACTGCCGTCGGCTTCCAGGCGAGCCCACTCATGGCTTTCGGc     >  2:357850/1‑134 (MQ=255)
                                                                                                                             aGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATACCCACGGTCACACTGCCGTCGGCTTCCAGGCGAGCCCACTCATGGCTTTCGGCaaa  >  4:184969/1‑135 (MQ=255)
                                                                                                                                |                                                                                                                                   
GGCTCTTCGTTCAGCAGTTCCGGGCTATCGGCCAGCTCTTCGTTGACCGCAATCACTTCGCCACCGACCGGGGCATAGATGTCCGAAGCGGCCTTGACCGACTCGACCACACCCGCAGCGTCGCCAGCGCCAAACACTTTGCCGACTTCAGCCAGCTCGACGAATACCACATCACCCAAGGCTTCCTGGGCGTGATCGCTGATACCCACGGTCACACTGCCGTCGGCTTCCAGGCGAGCCCACTCATGGCTTTCGGCAAA  >  ACB122/5917380‑5917639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑