Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB122	5,952,038	T→C	6.0%	L929P (CTG→CCG)	CJ019_05572 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB122	5,952,038	0	T	C	6.0%	212.3 / 3.6	66	L929P (CTG→CCG)	CJ019_05572	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (30/32);  new base C (2/2);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

AGCAGCTGTACGCGGTGGTGGCCCGGCAGATCATCGGTCAGCGCCGTGGCGCAGAGCGGTACCGGTGCTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCACAAGACTGCTGTGGAACAGCCTTGTGCTGCAAATGGGTCGCGAAGCGGCCC  >  ACB122/5951906‑5952169                                                                                                                                     |                                                                                                                                    agctgcTGTACGCGGGGGTGGCCCGGCAGATCATCGGTCAGCGCCGTGGCGCAGAGCGGTACCGGTGCTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAg                                                                                                                                  <  7:159856/136‑1 (MQ=255) agcagcGGTACGCGGTGGGGGCCCGGCAGATCATCGGTCAGCGCCGGGGCGCAGAGCGGTACCGGTGCTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAg                                                                                                                                  <  7:159854/136‑1 (MQ=255)  gcagcTGTACGCGGTGGTGGCCCGGCAGATCATCGGTCAGCGCCGTGGCGGAGAGCGGTACCGGTGCTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAg                                                                                                                                  <  1:102870/135‑1 (MQ=255)      cTGTACGCGGTGGTGGCCCGGCAGATCATCGGTCAGCGCCGTGGCGCAGAGCGGTACCGGTGCTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACa                                                                                                                             <  7:166273/136‑1 (MQ=255)         tACGCGGTGGTGGCCCGGCAGATCATCGGTCAGCGCCGTGGCGCAGAGCGGTACCGGTGCTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCACCGCAGGTCTACCTGAGCTACAAGc                                                                                                                          >  7:151941/1‑136 (MQ=255)           cgcgGTGGTGGCCCGGCAGATCATCGGTCAGCGCCGTGGCGCAGAGCGGTACCGGTGCTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCg                                                                                                                         >  6:185825/1‑135 (MQ=255)                 ggtggCCCGGCAGATCATCGGTCAGCGCCGTGGCGCAGAGCGGTACCGGTGCTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCt                                                                                                                   >  2:353100/1‑135 (MQ=255)                 ggtggCCCGGCAGATCATCGGTCAGCGCCGTGGCGCAGAGCGGTACCGGTGCTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTg                                                                                                                  <  7:313404/136‑1 (MQ=255)                   tggCCCGGCAGATCATCGGTCAGCGCCGTGGCGCAGAGCGGTACCGGTGCTATATCACCGCCCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCt                                                                                                                   >  1:27204/1‑133 (MQ=255)                   tggCCCGGCAGATCATCGGTCAGCGCCGTGGCGCAGAGCGGTACCGGTGCTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCt                                                                                                                   >  4:110186/1‑133 (MQ=255)                   tggCCCGGCAGATCATCGGTCAGCGCCGTGGCGCAGAGCGGTACCGGTGCTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTg                                                                                                                  >  8:43851/1‑134 (MQ=255)                    ggCCCGGCAGATCATCGGTCAGCGCCGTGGCGCAGAGCGGTACCGGTGCTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGa                                                                                                                <  2:327841/135‑1 (MQ=255)                    ggCCCGGCAGATCATCGGTCAGCGCCGTGGCGCAGAGCGGTACCGGTGCTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAg                                                                                                               >  8:217835/1‑136 (MQ=255)                        cGGCAGATCATCGGTCAGCGCCGTGGCGCAGAGCGGTACCGGTGCTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAg                                                                                                            <  6:232342/135‑1 (MQ=255)                        cGGCAGATCATCGGTCAGCGCCGTGGCGCAGAGCGGTACCGGTGCTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAg                                                                                                            >  5:232342/1‑135 (MQ=255)                             gATCATCGGTCAGCGCCGTGGCGCAGAGCGGTACCGGTGCTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCttt                                                                                                       >  7:212166/1‑135 (MQ=255)                             gATCATCGGTCAGCGCCGTGGCGCAGAGCGGTACCGGTGCTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCtt                                                                                                        >  6:40276/1‑134 (MQ=255)                                  tcGGTCAGCGCCGTGGCGCAGAGCGGTACCGGTGCTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCCGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGaa                                                                                                    >  2:91238/1‑133 (MQ=255)                                                  cgcAGAGCGGTACCGGTGCTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGt                                                                                  >  5:382418/1‑135 (MQ=255)                                                      gagCGGTACCGGTGCTATATCCCCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCATGCTTTGAATGAGGGGCTGGAACAGGTTTTg                                                                              <  4:307670/135‑1 (MQ=255)                                                          ggTACCGGTGCTATATCACCGACCGGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAATCGGGTGCTGGAGCAGGCTTTGAATGAGGTGCTGGAACAGGTTTTGgc                                                                            >  4:245072/1‑133 (MQ=255)                                                               cGGTGCTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCACCGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGgc                                                                     >  6:325537/1‑135 (MQ=255)                                                               cGGTGCTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAgg                                                                                               <  3:111832/109‑1 (MQ=255)                                                               cGGTGCTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAgg                                                                                               >  4:111832/1‑109 (MQ=255)                                                               cGGTGCTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGgc                                                                     >  1:137235/1‑135 (MQ=255)                                                                    cTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGTGCTGGAACAGGTTTTGGCGCAGGGCGCCt                                                                 >  3:348332/1‑134 (MQ=255)                                                                    cTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCt                                                                 >  3:227052/1‑134 (MQ=255)                                                                    cTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTg                                                                >  8:4021/1‑135 (MQ=255)                                                                        atCACCGTCCTGGAGTTGTCCGAGGTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAAc                                                             <  5:17211/134‑1 (MQ=255)                                                                        atCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAAc                                                             <  5:329661/134‑1 (MQ=255)                                                                         tCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCGGAACTc                                                           >  1:362020/1‑135 (MQ=255)                                                                            ccGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCgca                                                        <  2:287009/135‑1 (MQ=255)                                                                               aCCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGTGGCAACGCAGGTCTACCTGAGCTACAACCGGGTGCTGCAGGAGGATTTGAATGAGGGGCTGGATCAGGTTTTTGCGAAGGGCGCGTGAACTCgcaca                                                      >  4:264770/1‑132 (MQ=255)                                                                               aCCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCgcagca                                                     >  7:320496/1‑135 (MQ=255)                                                                               aCCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCgcagca                                                     >  8:288756/1‑135 (MQ=255)                                                                               aCCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCgcagc                                                      >  4:97942/1‑134 (MQ=255)                                                                                 cTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCACaa                                                  <  7:4021/136‑1 (MQ=255)                                                                                  tGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGcac                                                    >  8:20390/1‑133 (MQ=255)                                                                                  tGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCACaa                                                  <  3:110186/135‑1 (MQ=255)                                                                                      tttGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCACAAGActg                                             <  8:284726/136‑1 (MQ=255)                                                                                       ttGTCCGAGCTTCTGGAAGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCAGGAGCAGGCTTAGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCACAAGActg                                             <  6:153155/135‑1 (MQ=255)                                                                                       ttGTCCGAGCTGCTGGATGCCCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCACAAGActg                                             <  6:101765/135‑1 (MQ=255)                                                                                       ttGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCACAAGActgc                                            >  7:244901/1‑136 (MQ=255)                                                                                       ttGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCACAAGActg                                             <  8:212166/135‑1 (MQ=255)                                                                                           ccGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCACAAGACTGCtgtg                                        <  7:106254/136‑1 (MQ=255)                                                                                           ccGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCACAAGACTGCtgtg                                        <  7:349308/136‑1 (MQ=255)                                                                                            cGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAg                                                                                                            >  8:25926/1‑67 (MQ=255)                                                                                            cGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAg                                                                                                            <  7:25926/67‑1 (MQ=255)                                                                                            cGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCACAAGACTGCtgtg                                        <  1:353100/135‑1 (MQ=255)                                                                                             gAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCACAAGACTGCtgtg                                        <  6:382418/134‑1 (MQ=255)                                                                                              aGCTGCTGGATGACCGGCTGGGGGCAACGCATGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCACAAGACTGCTGTGGa                                      <  6:221657/135‑1 (MQ=255)                                                                                              aGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCACAAGACTGCTGTGGa                                      <  2:99636/135‑1 (MQ=255)                                                                                                 tgctgGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCACAAGACTGCTGTGGAACa                                   <  5:40276/135‑1 (MQ=255)                                                                                                   ctgGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCACAAGACTGCTGTGGAACAGc                                 <  5:325537/135‑1 (MQ=255)                                                                                                      gATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCACaa                                                  >  7:346836/1‑115 (MQ=255)                                                                                                      gATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCACaa                                                  <  8:346836/115‑1 (MQ=255)                                                                                                      gATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCACAAGACTGCTGTGGAACAGCCtt                              <  2:14825/135‑1 (MQ=255)                                                                                                           ccGGCTGGGGGCAACGCAGGTCTACCCGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAAc                                                             >  3:132749/1‑99 (MQ=255)                                                                                                           ccGGCTGGGGGCAACGCAGGTCTACCCGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAAc                                                             >  3:139405/1‑99 (MQ=255)                                                                                                           ccGGCTGGGGGCAACGCAGGTCTACCCGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAAc                                                             <  4:132749/99‑1 (MQ=255)                                                                                                           ccGGCTGGGGGCAACGCAGGTCTACCCGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAAc                                                             <  4:139405/99‑1 (MQ=255)                                                                                                                     gCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCACAAGACTGCTGTGGAACAGCCTTGTGCTGCAAATGGGTc              <  8:315981/136‑1 (MQ=255)                                                                                                                          gCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCACAAGACTGCTGTGGAACAGCCTTGTGCTGCAAATGGGTCGCGaa         <  8:244901/136‑1 (MQ=255)                                                                                                                            aGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCACAAGACTGCTGTGGAACAGCCTTGTGCTGCAAATGGGTCGCGAAg        >  5:171248/1‑135 (MQ=255)                                                                                                                                 tACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCCCAAGACTGCTGTGGAACAGCCTTGTGCTGCAAATGGGTCGCGAAGCGGcc   >  4:391867/1‑135 (MQ=255)                                                                                                                                    cTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCACAAGACTGCTGTGGAACAGCCTTGTGCTGCAAATGGGTCGCGAAGCGGccc  <  1:255751/133‑1 (MQ=255)                                                                                                                                     |                                                                                                                                    AGCAGCTGTACGCGGTGGTGGCCCGGCAGATCATCGGTCAGCGCCGTGGCGCAGAGCGGTACCGGTGCTATATCACCGACCTGGATTTGTCCGAGCTGCTGGATGACCGGCTGGGGGCAACGCAGGTCTACCTGAGCTACAAGCGGGTGCTGGAGCAGGCTTTGAATGAGGGGCTGGAACAGGTTTTGGCGCAGGGCGCCTGAACTCGCAGCACAAGACTGCTGTGGAACAGCCTTGTGCTGCAAATGGGTCGCGAAGCGGCCC  >  ACB122/5951906‑5952169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑