Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 6,020,344 T→G 49.7% S57R (AGC→CGC)  ycaD_4 ← putative MFS‑type transporter YcaD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1226,020,3440TG49.7% 4.1 / 19.4 33S57R (AGC→CGC) ycaD_4putative MFS‑type transporter YcaD
Reads supporting (aligned to +/- strand):  ref base T (7/8);  new base G (5/11);  total (13/20)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.38e-01

GCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCG‑CCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGC  >  ACB122/6020215‑6020474
                                                                                                                                 |                                                                                                                                   
gCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCCCAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGGCCGCCAGGCG‑cc                                                                                                                              >  8:356878/1‑136 (MQ=255)
gCCGCAGCAGCAGCCATAACGGGAACCTCGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGgcg‑c                                                                                                                               <  3:1256/135‑1 (MQ=255)
  cGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCGGCTGGCG‑CCgg                                                                                                                            >  8:308095/1‑136 (MQ=255)
  cGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCG‑CCgg                                                                                                                            <  8:290128/136‑1 (MQ=255)
  cGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCGGCGGGCG‑ccc                                                                                                                             >  6:317535/1‑134 (MQ=255)
       cagcagcCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCCGCGGGCG‑CCGGcccc                                                                                                                        >  5:323649/1‑133 (MQ=255)
                  aCGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCGGCTGGCG‑CCCGCCCGTACGCCCACCGc                                                                                                            >  8:336025/1‑136 (MQ=255)
                    gggTAAGTCGGCAGCAGGGCGAGCAGGCCAAGCGCCAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCAAACCGGCCAGGCGGCTGGCCAGGCTGGCG‑CGGGCCAGAACGCCCAACGCCg                                                                                                          <  7:324705/136‑1 (MQ=255)
                          cTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGCCGCATCGCCGAAGGTACGCCAACCCAACCTGACAGGCGTCTGGCCAGCCTGGCG‑CCCGCCCTTACGCCAAACCCCGGCaatt                                                                                                    >  8:314140/1‑133 (MQ=255)
                                        gAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCGGCTGGCG‑CCGGCCAGTACGCCCACCGCCCGCCTTGCCGCCCTCCCGcc                                                                                       >  1:130829/1‑135 (MQ=255)
                                           cAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCGGCGGGCG‑CCGGCCCGTACGCCCCACGCCCGCATTGCCGCCCTCACGCCGAt                                                                                    >  4:224086/1‑135 (MQ=255)
                                               caactCGACGGCGCCCCGCCCCACAGGCACCGCCGCATCGCGGAAGGAACGCCAACCCACCCTGCCTGGCGGCGGGCCCGGGGGGGG‑CCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCg                                                                                <  4:332245/131‑1 (MQ=255)
                                                cAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGGCCGGCTGGCG‑CCCGGCCCTACGCCCCACCCCCGCATTTCCCCCAACACGCCCAAGGCgca                                                                              >  8:375646/1‑134 (MQ=255)
                                                      ccAGCGCCCCGCCCCACAGCATCAGGCGCATCGCCGAAGTTACGCCAACCCAACCTGCAAGGCGGCTGGCAAGGCGGGGG‑CGGGCAAGAACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGc                                                                         <  6:177923/134‑1 (MQ=255)
                                                           gccccgccccCCCGGCACCGGCGCTGCGCCGAAGGACCCCCACCCCACCCTGCCGGGGGGCGGGCCCGGCGGGCG‑CGGGCCTGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGc                                                                   <  7:300547/136‑1 (MQ=255)
                                                           gccccgccccACAGGCACCGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCG‑CCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGc                                                                   <  8:237085/136‑1 (MQ=255)
                                                            ccccgccccACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCCGCTGGGG‑CCCGCCAGGACGCCCAACGCCGGCCTTGCCGCCCTCACGCCGCTGGCGACGCTGCCGGAGcc                                                                  >  7:46387/1‑136 (MQ=255)
                                                                         gcacaggcGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGCCCCGCGGGCGCCCGGCCGGT‑CGCCCAACCCCGGCAGTTCCGCCAACACCCCGCATGCGGAGCTGCCCTAGGCCCCGgccgcccg                                                      >  5:273713/1‑133 (MQ=255)
                                                                           accggcGCAGCCCCTACGGTCCCCCACCCCACCCTGCCGGGGGGCTGGCCAGGCGGGCG‑CCGGCAAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGgc                                                    <  1:303026/132‑1 (MQ=255)
                                                                           acaggcGCATCGCCGACGGTAGGCCACCCCAACCTGCCAGGCGGCTGGCCAGGCCGGCG‑CCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGgc                                                    <  3:160598/135‑1 (MQ=255)
                                                                            caggcGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCG‑CCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGgcgc                                                  <  7:287181/136‑1 (MQ=255)
                                                                                      gCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCCGGCTGGCG‑CCGGCCCCTACGCCCCACGCCGGCCTTTCCGCCATCACGCCGATGGCGCAGCTGCCGTAGCCCCAGGCCGCCCGGCGCAggggcccc                                         >  6:168467/1‑130 (MQ=255)
                                                                                      gCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCGGGGCCCGCGGGCG‑CCCGCCCGGGCGCCCCCCCCCCGCCATGCCGCCCACACCCCCCGGGCGCAGCTGGCCTAGCCCCCCGCCGCCCCGCGCCgggccccc                                         >  4:284316/1‑130 (MQ=255)
                                                                                              accccaaacccacccGGCCCGGGGGCGGGCCGGGGGGGCG‑CGGGCCAGTACCCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCAt                                 <  3:284316/129‑1 (MQ=255)
                                                                                              aaccccaccccaccCGGCCCGGCGGCGCGCCAGGCGGGCG‑CCGCCCAGTACCCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCAt                                 <  1:116920/131‑1 (MQ=255)
                                                                                                         acccTGCCAGGCGGCTGGCCAGGCTGGCG‑CCGGCCAGTAGGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                     <  8:361228/134‑1 (MQ=255)
                                                                                                         aaccTGCCTGGGGGCGGGCCAGGCTGGCG‑CCGGCCTGTCCGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAg                     <  8:248459/136‑1 (MQ=255)
                                                                                                              gCCAGGCGGCTGGCCAGGCTGGCG‑CCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCa                <  7:92711/136‑1 (MQ=255)
                                                                                                                 cggcggcTGCCCGGGGGGGCG‑CGGCCCATAACCCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGc              <  6:323649/134‑1 (MQ=255)
                                                                                                                   gcggcgGGCCCGGCGGGCG‑CCGGCAAGAACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGccc            <  5:168467/135‑1 (MQ=255)
                                                                                                                    gggcGGGCCAGGGGGGCG‑CCGGCCATTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCg           <  6:280854/134‑1 (MQ=255)
                                                                                                                      gcTGGCCAGGCGGGCG‑CCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGac         <  3:246849/135‑1 (MQ=255)
                                                                                                                             aGGCGGGCG‑CCGGCAATAACGCCAAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGc  <  2:268003/135‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                   
GCCGCAGCAGCAGCCATAACGGGTAACTAGGCAGCAGCGCGAGCAGGCCAATCGACAGCGCCCCGCCCCACAGGCACAGGCGCATCGCCGAAGGTACGCCAACCCAACCTGCCAGGCGGCTGGCCAGGCTGGCG‑CCGGCCAGTACGCCCAACGCCGGCATTGCCGCCATCACGCCGATGGCGAAGCTGCCGTAGCCCCAGGCCTCCAGGCGCAGGGACACCAGCGGCATGCTCACGCCAAGCGCCAGCCCGACACTGAGC  >  ACB122/6020215‑6020474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑