Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 613,718 C→G 8.7% A8P (GCC→CCC)  ompR_3 ← Transcriptional regulatory protein OmpR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB122613,7180CG 8.7% 179.9 / 2.1 70A8P (GCC→CCC) ompR_3Transcriptional regulatory protein OmpR
Reads supporting (aligned to +/- strand):  ref base C (22/41);  new base G (4/3);  total (26/44)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCAGCACCAGTTCGAACTGAGATTGCTGTATGGCATTCAGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCATGCTGCACCCTGGTTTTCG  >  ACB122/613585‑613850
                                                                                                                                     |                                                                                                                                    
agcagcACCAGTTCGAACTGAGATTGCTGTATGGCATTCAGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGc                                                                                                                                      <  2:273015/134‑1 (MQ=255)
     caCCTGTTCGAACTGAGATTGCTGTATGGCATTCAGGGCCGGGGGGCCGATGGCGCAGGGGGGCTCCATGAAACGGCGGTTGCGGAAATGGCGGTCCTGGGCCTGAAGCAGGCGTGGGTCGTCTTCGGGAAGgaga                                                                                                                               <  7:388981/136‑1 (MQ=255)
     caCCAGTTCGAACTGAGATTGCTGTATGGCATTCAGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGATATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTAGCCaacgcg                                                                                                                                >  7:317155/1‑131 (MQ=255)
     caCCAGTTCGAACTGAGATTGCTGTATGGCATTCAGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGGAAGgaga                                                                                                                               <  7:388983/136‑1 (MQ=255)
     caCCAGTTCGAACTGAGATTGCTGTATGGCATTCAGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGgag                                                                                                                                >  7:301442/1‑135 (MQ=255)
        cAGTTCGAACTGAGATTGCTGTATGGCATTCAGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAg                                                                                                                             >  5:134070/1‑135 (MQ=255)
         aGTTCGAACTGAGATTGCTGTATGGCATTCAGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTg                                                                                                                           <  7:260566/136‑1 (MQ=255)
          gTTCGAACTGAGATTGCTGTATGGCATTCAGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAg                                                                                                                             >  2:281421/1‑133 (MQ=255)
          gTTCGAACTGAGATTGCTGTATGGCATTCAGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTg                                                                                                                           >  3:352685/1‑135 (MQ=255)
                          cTGTATGGCATTCAGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGa                                                                                                            <  4:21548/134‑1 (MQ=255)
                           tGTATGGCATTCAGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGcc                                                                                                         >  8:264353/1‑136 (MQ=255)
                            gTATGGCATTCAGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTAGGGAATGAGAAGTGAGGTGGGCACGGCGAGcc                                                                                                         >  3:137802/1‑135 (MQ=255)
                             tATGGCATTCAGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCt                                                                                                        >  6:82416/1‑135 (MQ=255)
                                 gCATTCAGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTtga                                                                                                     >  3:120036/1‑134 (MQ=255)
                                  cATTCAGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGgaga                                                                                                                               <  5:235128/107‑1 (MQ=255)
                                  cATTCAGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGgaga                                                                                                                               >  6:235128/1‑107 (MQ=255)
                                       aGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAAt                                                                                              >  5:378852/1‑135 (MQ=255)
                                       aGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAAt                                                                                              >  3:252203/1‑135 (MQ=255)
                                       aGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTAGGCAAGGAGAAGTGAGGGGGGCACGGCGAGCCTTTATGAGAAt                                                                                              >  2:80807/1‑135 (MQ=255)
                                          ggcTGGGTGGCGCTGGCGGAGTGGTGCAGGAGTTCAGGGCGGTGGGGGAAATGGCGGTCCAGGTCCTGAAGCAGGGGTGGGTCGTCTTCGTCAATGAGATGTGAGTTGGGCACGGCGAGGCTTGATGAGTAtttt                                                                                           <  2:96070/133‑1 (MQ=255)
                                          gCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTTGGTATTCTTAGTCAATTAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAAtttt                                                                                           <  2:280267/135‑1 (MQ=255)
                                          gCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTGGGGGGGTAGGGGGAGTGAGGTGGGCCCGGGGCGGCGTGATGGGAAtttt                                                                                           >  5:3496/1‑135 (MQ=255)
                                          gCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAAtttt                                                                                           <  1:324635/135‑1 (MQ=255)
                                          gCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAAtttt                                                                                           <  5:82416/135‑1 (MQ=255)
                                          gCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAAttt                                                                                            >  2:90595/1‑134 (MQ=255)
                                          gCCGGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAAtttt                                                                                           <  4:47654/135‑1 (MQ=255)
                                              gggTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCGTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCGTGATGAGAATTTTTa                                                                                         >  4:72330/1‑133 (MQ=255)
                                               ggTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTAGTGGTAGGGAAGGGGGAGAGAGGGTGGCGCGGCGGGCCTTGATGAGAAattttttaa                                                                                      >  1:150845/1‑131 (MQ=255)
                                                  gCCGCTGGCGCAGGCGTGCACCAGGAACCCGCGGCTGCGGAAAAGGCGGTCCAGGGCCTGAAGCAGGCGTGGGGCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGtt                                                                                   <  1:357495/135‑1 (MQ=255)
                                                  gCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGtt                                                                                   <  1:281421/135‑1 (MQ=255)
                                                    cggtGGCGGCGGCGTGCACGTGGAAAGGGCGGTTGTGTAATTGGGGGTGCAGGTCGTGAAGGTGGGGTGGTTCGTTTTGGGCAAGGAGAAGTTTGGTGGGCACGGCGAGCCTTTATTAGAATTTTTTTAAGtttc                                                                                 <  2:150845/132‑3 (MQ=255)
                                                    cGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTTGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGc                                                                                 <  2:95976/135‑1 (MQ=255)
                                                    cGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTg                                                                                  <  6:378852/134‑1 (MQ=255)
                                                     gCTGGCGCAGGCGGGCACCAGGAAACCGCGGTTGCTGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGTGTCGTCTTCGGCATGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTTCGa                                                                               <  8:108216/136‑1 (MQ=255)
                                                      cTGGCGCAGGAGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGa                                                                               <  4:352685/135‑1 (MQ=255)
                                                        ggCGCAGGCGTGCACCAGGAAACCGCGGTTGCGTAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAat                                                                             <  6:49187/135‑1 (MQ=255)
                                                          cgcAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATTGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAatcat                                                                          <  7:245664/136‑1 (MQ=255)
                                                             aGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATccc                                                                       <  8:103599/136‑1 (MQ=255)
                                                             aGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATcc                                                                        <  6:384242/135‑1 (MQ=255)
                                                                cGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCAc                                                                     <  1:220243/135‑1 (MQ=255)
                                                                       cAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGCGCCTTGATGAGAATTTTTTTCAGTTGCGAATCATCCCACTGGCt                                                                >  1:3786/1‑133 (MQ=255)
                                                                        aGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAg                                                            <  7:273187/136‑1 (MQ=255)
                                                                         ggAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAg                                                            <  3:63010/135‑1 (MQ=255)
                                                                            aaCCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCAc                                                                                                                 <  2:135586/79‑1 (MQ=255)
                                                                            aaCCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCAc                                                                                                                 >  1:135586/1‑79 (MQ=255)
                                                                               cgcgGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAgg                                                     <  7:87565/136‑1 (MQ=255)
                                                                                gcgGTTGCGGAAATGGCGGTCCAGGTCCTGAAGGAGGCGTGGGTCGTGTTGGGCAAGGAGAAGTGAGGTGGGCACGGCGAGGCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAgg                                                     <  1:80807/135‑1 (MQ=255)
                                                                                gcgGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAgg                                                     <  1:90595/135‑1 (MQ=255)
                                                                                gcgGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAgg                                                     <  4:120036/135‑1 (MQ=255)
                                                                                gcgGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCGTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAggg                                                    >  7:12982/1‑136 (MQ=255)
                                                                                gcgGGTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTATTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAgg                                                     <  6:1293/135‑1 (MQ=255)
                                                                                gcgGGTGCCGAAATGGCGGGCCAGGTCCTGAAGCAGGCGTGGGGCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAgg                                                     <  6:116024/135‑1 (MQ=255)
                                                                                  ggTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATAAGTTGCGAATACTCCCACTGGCGGCAGGGAAAGGGc                                                   >  2:69079/1‑135 (MQ=255)
                                                                                    ttGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCGTCGGGAAGGAGAAGTGAGGTGGGCACGGCGCGCCTTGAGGAGAATTTTTATCAGTTGCGAATCATGCCACTTGCGGCAGGGTAAGGGcca                                                 >  6:327108/1‑133 (MQ=255)
                                                                                      gCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCGTCGGCAAGGAGAATTGAGGTGGGCACGGCCAGCCATCATGAGAAATTTTATAAGTTGAGAATCATCCCACTGGCTTCACGGAAAGGGCAAct                                               >  4:293602/1‑134 (MQ=255)
                                                                                        ggAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGcc                                             <  4:220167/135‑1 (MQ=255)
                                                                                        ggAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGcc                                             <  2:94571/135‑1 (MQ=255)
                                                                                          taaTGGCGGTCCTGGTCCTGAAGCAGGCGTGGGTCGTCTTCGTGAAGGAGAAGTGAGGTTGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCtt                                           <  2:289262/134‑1 (MQ=255)
                                                                                          aaaTGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCtt                                           <  6:3496/135‑1 (MQ=255)
                                                                                          aaaTGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTg                                          <  7:35738/136‑1 (MQ=255)
                                                                                           aaTGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTg                                          <  1:294881/135‑1 (MQ=255)
                                                                                                  gTCCAGGTCCTTAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGtt                                   <  4:137802/135‑1 (MQ=255)
                                                                                                     cAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTaag                                <  6:62964/135‑1 (MQ=255)
                                                                                                         tCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGt                           <  8:311860/136‑1 (MQ=255)
                                                                                                           cTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGCAGTGAGGTGGTCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTTCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTa                         <  8:362740/136‑1 (MQ=255)
                                                                                                               aGCAGGCGTGGGTCGTCTTCGGCACGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTCTCAGTAGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCa                     <  8:342489/136‑1 (MQ=255)
                                                                                                                      gTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCAtgctgc               >  3:143794/1‑135 (MQ=255)
                                                                                                                      gTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCATGCTGCa              >  7:61772/1‑136 (MQ=255)
                                                                                                                                   ggCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCATGCTGCACCCTGGttt     <  3:293602/132‑1 (MQ=255)
                                                                                                                                   ggCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCATGCTGCACCCTGGttt     <  1:209920/132‑1 (MQ=255)
                                                                                                                                   ggCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGCTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCATGCTGCACCCAGGTTTTCg  >  4:288249/1‑135 (MQ=255)
                                                                                                                                     |                                                                                                                                    
AGCAGCACCAGTTCGAACTGAGATTGCTGTATGGCATTCAGGGCCTGGGTGCCGCTGGCGCAGGCGTGCACCAGGAAACCGCGGTTGCGGAAATGGCGGTCCAGGTCCTGAAGCAGGCGTGGGTCGTCTTCGGCAAGGAGAAGTGAGGTGGGCACGGCGAGCCTTGATGAGAATTTTTATCAGTTGCGAATCATCCCACTGGCTGCAGGGAAAGGGCAACGCCTTGATGTGTTAAGAAGGTTAATCATGCTGCACCCTGGTTTTCG  >  ACB122/613585‑613850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑