Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB122 6,144,061 C→T 6.1% A137V (GCG→GTG)  bpsA → N(4)‑bis(aminopropyl)spermidine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1226,144,0610CT 6.1% 210.8 / 3.5 68A137V (GCG→GTG) bpsAN(4)‑bis(aminopropyl)spermidine synthase
Reads supporting (aligned to +/- strand):  ref base C (33/29);  new base T (2/2);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01

GGCGATTCGGTGAAAAACTAGCGCCCAAGATCGAAATCACTGCCCATCTCTACAACGTGGCTGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTCCATGGACACAAGAGGTGCTCAGAGATAC  >  ACB122/6143935‑6144193
                                                                                                                              |                                                                                                                                    
ggCGATTCGGTGAAAAACTAGCGCCCAAGATCGAAATCACTGCCCATCTCTACAACGTGGCTGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCGGGGGAGCGAGCAAcg                                                                                                                              >  5:220130/1‑135 (MQ=255)
   gATTCGGTGAAAAACTAGCGCCCAAGATCGAAATCACTGCCCATCTCTACAACGTGGCTGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAAcggcgg                                                                                                                          <  8:29990/136‑1 (MQ=255)
   gATTCGGTGAAAAACTAGCGCCCAAGATCGAAATCACTGCCCATCTCTACAACGTGGCTGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAAcggcgg                                                                                                                          <  7:51002/136‑1 (MQ=255)
    aTTCGGTGAAAAACTAGCGCCCAAGATCGAAATCACTGCCCATCTCTACAACGTGGCTGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAAcggcgg                                                                                                                          <  6:367610/135‑1 (MQ=255)
    aTTCGGTGAAAAAATAGCGCCCAAGATCGAAATCACTGCCCATCTCTACAACGTGGCTGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAAcggcgg                                                                                                                          <  5:78602/135‑1 (MQ=255)
     ttCGGTGAAAAACTAGCGCCCAAGATCGAAATCACTGCCCATCTCTACAACGTGGCTGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGTGAGCACcggcgg                                                                                                                          >  2:342714/1‑134 (MQ=255)
     ttCGGTGAAAAACTAGCGCCCAAGATCGAAATCACTGCCCATCTCTACAACGTGGCTGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACggcggc                                                                                                                         >  8:81831/1‑135 (MQ=255)
        ggTGAAAAACTAGCGCCCAAGATCGAAATCACTGCCCATCTCTACAACGTGGCTGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGCGCAACGGCGGcc                                                                                                                        >  4:216792/1‑133 (MQ=255)
        ggTGAAAAACTAGCGCCCAAGATCGAAATCACTGCCCATCTCTACAACGTGGCTGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGcc                                                                                                                        >  6:271214/1‑133 (MQ=255)
                       cccAAGATCGAAATCACTGCCCATCTCTACAACGTGGCTGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTc                                                                                                      >  8:150247/1‑136 (MQ=255)
                       cccAAGATCGAAATCACTGCCCATCTCTACAACGTGGCTGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTc                                                                                                      >  7:263430/1‑136 (MQ=255)
                       cccAAGATCGAAATCACTGACCATCTCTACAACGTGGCTGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCAGGGGAGCGAGCAACGGCGGCCAAAGAGTGACGGCGtta                                                                                                       >  2:214770/1‑134 (MQ=255)
                          aaGATCGAAATCACTGCCCATCTCTACAACGTGGCTGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTg                                                                                                    >  8:203733/1‑135 (MQ=255)
                                     cACTGCCCATCTCTACAACGTGGCTGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAg                                                                                         <  5:305145/135‑1 (MQ=255)
                                         gCCCATCTCTACAACGTGGCTGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAgg                                                                                    >  8:63087/1‑136 (MQ=255)
                                           ccATCTCTACAACGTGGCTGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAgg                                                                                    >  4:291316/1‑134 (MQ=255)
                                                     aaCGTGGCTGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAAcg                                                                           <  3:373467/133‑1 (MQ=255)
                                                       cGTGGCTGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAAcgag                                                                         >  4:352443/1‑133 (MQ=255)
                                                          ggCTGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAgg                                                                    <  1:262653/135‑1 (MQ=255)
                                                            cTGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGTGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTc                                                                  >  6:196306/1‑135 (MQ=255)
                                                            cTGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCt                                                                 >  7:52928/1‑136 (MQ=255)
                                                            cTGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCAGCCCTCGTGAGCGAGCAACGCCGGCCAAAGTGTGACGCCGTTTATGGAACGAGGCAGCCAGTCAACCAACGAGCGAGGTc                                                                  >  6:243961/1‑135 (MQ=255)
                                                             tGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCACCCAACGAGCGAGGTCt                                                                 >  3:104474/1‑135 (MQ=255)
                                                             tGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCt                                                                 >  4:319834/1‑135 (MQ=255)
                                                             tGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCAGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGTAACTAGTCAGCCAGGAAACCAACGAGCGCGGTCt                                                                 >  4:81600/1‑135 (MQ=255)
                                                               aCCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTg                                                               >  2:206974/1‑135 (MQ=255)
                                                               aCCCTCTACCTGCCGAACATTTTGCGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCTAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGCTCATg                                                               >  2:284601/1‑135 (MQ=255)
                                                                    ctACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGCGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCg                                                         >  7:49373/1‑136 (MQ=255)
                                                                       ccTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGtt                                                       >  2:253582/1‑135 (MQ=255)
                                                                        cTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGACCGAGGCCGCCAGGCACCCCACGAGCGCGGTCTTGGCATCGTTg                                                      >  2:362639/1‑135 (MQ=255)
                                                                        cTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGt                                                     <  7:150247/136‑1 (MQ=255)
                                                                             gAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGcc                                                                                       >  1:317602/1‑97 (MQ=255)
                                                                             gAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGcc                                                                                       <  2:317602/97‑1 (MQ=255)
                                                                              aaCATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGc                                               >  8:356178/1‑136 (MQ=255)
                                                                              aaCATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCATGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTg                                                >  6:23517/1‑135 (MQ=255)
                                                                               aCATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGc                                               >  4:276194/1‑135 (MQ=255)
                                                                                     tGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATa                                          <  3:248027/134‑1 (MQ=255)
                                                                                        ggCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGAGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGTCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCg                                     <  8:263430/136‑1 (MQ=255)
                                                                                         gCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCg                                     <  3:370806/135‑1 (MQ=255)
                                                                                         gCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCg                                     <  3:184189/135‑1 (MQ=255)
                                                                                         gCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCg                                     <  3:291316/135‑1 (MQ=255)
                                                                                               gacgGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGATCGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCt                                <  2:216623/134‑1 (MQ=255)
                                                                                               gacgGCGTTCATATCAACCCGCCCTGGGGAGCGAGCATCGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAAACAACGAGCGAGGTATTGGCATCGTTGTCATTGCCGATAACCCGGATCt                                <  1:214770/134‑1 (MQ=255)
                                                                                                 cgGCTTTCATATCAACCCGCCCTGGGGAGTGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTcc                             <  5:196306/135‑1 (MQ=255)
                                                                                                   gCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTCCAt                           <  2:318543/135‑1 (MQ=255)
                                                                                                       tCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATGGCCGATAACCCGGATCTTCCATGGac                       >  5:105071/1‑135 (MQ=255)
                                                                                                        cATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTCCATGGa                        <  3:352443/133‑1 (MQ=255)
                                                                                                          tatCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTCCATGGacac                     <  4:148395/134‑1 (MQ=255)
                                                                                                           atCAACCCGCCCTGGGGAGGGAGCAAGGGCGTCCAAAGTGTGTCGGGGTTTCTGTAACGATGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTAATTGCCGATAACCCGGATCTTCCTTGGACACaa                   <  5:158807/135‑1 (MQ=255)
                                                                                                           atCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTCCATGGACACaa                   <  2:208406/135‑1 (MQ=255)
                                                                                                               aCCCGCGCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTCCATGGACACAagag                <  1:253582/134‑1 (MQ=255)
                                                                                                                cccGGCCTGGGGAGCGAGCCACGGCGGCCAAACTGTGACGGCGTTTCTGGAACGAGGGAGCCACGGAACCAACGAGCGTGGTGGTGGCATCGGTGTCATTGCCGATAACCCGGATCTTCCATGGACACAAGAGGt              <  6:242302/135‑1 (MQ=255)
                                                                                                                 ccGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTCCATGGacac                     >  6:275262/1‑127 (MQ=255)
                                                                                                                 ccGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTCCATGGacac                     <  5:275262/127‑1 (MQ=255)
                                                                                                                 ccGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTCCATGGACACAAGAGGTg             >  3:5660/1‑135 (MQ=255)
                                                                                                                   gCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTCCATGGACACAAGAGGTGCt           <  3:126949/135‑1 (MQ=255)
                                                                                                                     ccTGGTGAGCGAGCAACGGCGGCCAAAGTGTGACGTCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGATGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTCCATGGACACAAGAGGTGCTCa         <  3:23948/135‑1 (MQ=255)
                                                                                                                     ccTGGGGAGTGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTCCATGGACACAAGAGGTGCTCa         <  1:342714/135‑1 (MQ=255)
                                                                                                                      cTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGAGAACCCGGATCTTCCATGGACACAAGAGGTGCTc          >  6:41753/1‑133 (MQ=255)
                                                                                                                       tGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTCCATGGACACTAGAGGTGCTc          >  3:13511/1‑132 (MQ=255)
                                                                                                                       tGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTCCATGGACACAAGAGGt              <  4:101982/128‑1 (MQ=255)
                                                                                                                       tGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTCCATGGACACAAGAGGt              >  3:101982/1‑128 (MQ=255)
                                                                                                                       gggggAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTCCATGGACACAAGAGGTGCTc          >  1:104140/2‑132 (MQ=255)
                                                                                                                         gggAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTCCATGGACACAAGAGGTGCTCAGAGAt    <  7:203733/136‑1 (MQ=255)
                                                                                                                          ggagggagCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTCCATGGACACAAGAGGTGCTCagag      <  5:317757/133‑1 (MQ=255)
                                                                                                                          ggAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTCCATGGACACAAGAGGTGCTCagag      <  2:358343/133‑1 (MQ=255)
                                                                                                                          ggAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTCCATGGACACAAGAGGTGCTCAGAGAt    <  3:216792/135‑1 (MQ=255)
                                                                                                                          ggAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTCCATGGACACAAGAGGTGCTCAGAGAt    <  3:276194/135‑1 (MQ=255)
                                                                                                                          ggAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTCCATGGACACAAGAGGTGCTCAGAGAt    >  5:96802/1‑135 (MQ=255)
                                                                                                                            agcgagcAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTCCATGGACACAAGAGGTGCTCAGAGATac  >  2:230927/1‑135 (MQ=255)
                                                                                                                              |                                                                                                                                    
GGCGATTCGGTGAAAAACTAGCGCCCAAGATCGAAATCACTGCCCATCTCTACAACGTGGCTGACCCTCTACCTGCCGAACATTTTGGGGCCTACGACGGCTTTCATATCAACCCGCCCTGGGGAGCGAGCAACGGCGGCCAAAGTGTGACGGCGTTTCTGGAACGAGGCAGCCAGGCAACCAACGAGCGAGGTCTTGGCATCGTTGTCATTGCCGATAACCCGGATCTTCCATGGACACAAGAGGTGCTCAGAGATAC  >  ACB122/6143935‑6144193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑